Details of the Target

General Information of Target

Target ID LDTP06223
Target Name AT-rich interactive domain-containing protein 5B (ARID5B)
Gene Name ARID5B
Gene ID 84159
Synonyms
DESRT; MRF2; AT-rich interactive domain-containing protein 5B; ARID domain-containing protein 5B; MRF1-like protein; Modulator recognition factor 2; MRF-2
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MEPNSLQWVGSPCGLHGPYIFYKAFQFHLEGKPRILSLGDFFFVRCTPKDPICIAELQLL
WEERTSRQLLSSSKLYFLPEDTPQGRNSDHGEDEVIAVSEKVIVKLEDLVKWVHSDFSKW
RCGFHAGPVKTEALGRNGQKEALLKYRQSTLNSGLNFKDVLKEKADLGEDEEETNVIVLS
YPQYCRYRSMLKRIQDKPSSILTDQFALALGGIAVVSRNPQILYCRDTFDHPTLIENESI
CDEFAPNLKGRPRKKKPCPQRRDSFSGVKDSNNNSDGKAVAKVKCEARSALTKPKNNHNC
KKVSNEEKPKVAIGEECRADEQAFLVALYKYMKERKTPIERIPYLGFKQINLWTMFQAAQ
KLGGYETITARRQWKHIYDELGGNPGSTSAATCTRRHYERLILPYERFIKGEEDKPLPPI
KPRKQENSSQENENKTKVSGTKRIKHEIPKSKKEKENAPKPQDAAEVSSEQEKEQETLIS
QKSIPEPLPAADMKKKIEGYQEFSAKPLASRVDPEKDNETDQGSNSEKVAEEAGEKGPTP
PLPSAPLAPEKDSALVPGASKQPLTSPSALVDSKQESKLCCFTESPESEPQEASFPSFPT
TQPPLANQNETEDDKLPAMADYIANCTVKVDQLGSDDIHNALKQTPKVLVVQSFDMFKDK
DLTGPMNENHGLNYTPLLYSRGNPGIMSPLAKKKLLSQVSGASLSSSYPYGSPPPLISKK
KLIARDDLCSSLSQTHHGQSTDHMAVSRPSVIQHVQSFRSKPSEERKTINDIFKHEKLSR
SDPHRCSFSKHHLNPLADSYVLKQEIQEGKDKLLEKRALPHSHMPSFLADFYSSPHLHSL
YRHTEHHLHNEQTSKYPSRDMYRESENSSFPSHRHQEKLHVNYLTSLHLQDKKSAAAEAP
TDDQPTDLSLPKNPHKPTGKVLGLAHSTTGPQESKGISQFQVLGSQSRDCHPKACRVSPM
TMSGPKKYPESLSRSGKPHHVRLENFRKMEGMVHPILHRKMSPQNIGAARPIKRSLEDLD
LVIAGKKARAVSPLDPSKEVSGKEKASEQESEGSKAAHGGHSGGGSEGHKLPLSSPIFPG
LYSGSLCNSGLNSRLPAGYSHSLQYLKNQTVLSPLMQPLAFHSLVMQRGIFTSPTNSQQL
YRHLAAATPVGSSYGDLLHNSIYPLAAINPQAAFPSSQLSSVHPSTKL
Target Bioclass
Transcription factor
Family
ARID5B family
Subcellular location
Nucleus
Function
Transcription coactivator that binds to the 5'-AATA[CT]-3' core sequence and plays a key role in adipogenesis and liver development. Acts by forming a complex with phosphorylated PHF2, which mediates demethylation at Lys-336, leading to target the PHF2-ARID5B complex to target promoters, where PHF2 mediates demethylation of dimethylated 'Lys-9' of histone H3 (H3K9me2), followed by transcription activation of target genes. The PHF2-ARID5B complex acts as a coactivator of HNF4A in liver. Required for adipogenesis: regulates triglyceride metabolism in adipocytes by regulating expression of adipogenic genes. Overexpression leads to induction of smooth muscle marker genes, suggesting that it may also act as a regulator of smooth muscle cell differentiation and proliferation. Represses the cytomegalovirus enhancer.
Uniprot ID
Q14865
Ensemble ID
ENST00000279873.12
HGNC ID
HGNC:17362

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
AN3CA SNV: p.A358T .
C32 SNV: p.N770D .
CMK SNV: p.L1016V .
COLO792 SNV: p.S1075F .
CORL23 SNV: p.N683Y .
G402 SNV: p.Q1004H .
HCC1419 SNV: p.E470Q .
HCT15 SNV: p.D242N .
HEC1 Insertion: p.L695AfsTer31
SNV: p.K197N;p.C317Y		 .
HEC1B SNV: p.C317Y .
IGROV1 SNV: p.R1010W .
IM95 SNV: p.A1009G .
JURKAT SNV: p.L887P .
KARPAS422 SNV: p.Y19D; p.S88G .
KPNYN SNV: p.S544I .
LS180 SNV: p.R443H; p.P1003L; p.N1108T .
MDAMB468 SNV: p.G1155V .
MELHO SNV: p.L6F .
MFE296 Insertion: p.Q575NfsTer55 .
MIAPACA2 SNV: p.C1087Y .
NCIH196 SNV: p.S747C .
NCIH23 SNV: p.M332V .
REH Insertion: p.I213HfsTer18 .
TOV21G Deletion: p.G1064AfsTer43 .
YSCCC SNV: p.R407T .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 7 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C74(3.15)  LDD3470  [1]
IA-alkyne
 Probe Info  		N.A.  LDD0036  [2]
Lodoacetamide azide
 Probe Info  		C122(0.00); C300(0.00)  LDD0037  [2]
TFBX
 Probe Info  		N.A.  LDD0027  [3]
IPM
 Probe Info  		C317(0.00); C258(0.00)  LDD0147  [3]
AOyne
 Probe Info  		15.00  LDD0443  [4]
NAIA_5
 Probe Info  		N.A.  LDD2223  [5]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0215  AC10 HEK-293T C122(0.56)  LDD0813  [6]
 LDCM0216  AC100 HEK-293T C122(0.72)  LDD0814  [6]
 LDCM0217  AC101 HEK-293T C122(0.92)  LDD0815  [6]
 LDCM0218  AC102 HEK-293T C122(0.64)  LDD0816  [6]
 LDCM0219  AC103 HEK-293T C122(0.56)  LDD0817  [6]
 LDCM0220  AC104 HEK-293T C122(0.80)  LDD0818  [6]
 LDCM0221  AC105 HEK-293T C122(1.38)  LDD0819  [6]
 LDCM0222  AC106 HEK-293T C122(0.99)  LDD0820  [6]
 LDCM0223  AC107 HEK-293T C122(0.74)  LDD0821  [6]
 LDCM0224  AC108 HEK-293T C122(1.34)  LDD0822  [6]
 LDCM0225  AC109 HEK-293T C122(1.07)  LDD0823  [6]
 LDCM0226  AC11 HEK-293T C122(0.47)  LDD0824  [6]
 LDCM0227  AC110 HEK-293T C122(0.44)  LDD0825  [6]
 LDCM0228  AC111 HEK-293T C122(0.55)  LDD0826  [6]
 LDCM0229  AC112 HEK-293T C122(0.45)  LDD0827  [6]
 LDCM0237  AC12 HEK-293T C122(0.58)  LDD0835  [6]
 LDCM0246  AC128 HEK-293T C122(0.95)  LDD0844  [6]
 LDCM0247  AC129 HEK-293T C122(0.83)  LDD0845  [6]
 LDCM0249  AC130 HEK-293T C122(0.86)  LDD0847  [6]
 LDCM0250  AC131 HEK-293T C122(1.10)  LDD0848  [6]
 LDCM0251  AC132 HEK-293T C122(0.77)  LDD0849  [6]
 LDCM0252  AC133 HEK-293T C122(0.52)  LDD0850  [6]
 LDCM0253  AC134 HEK-293T C122(0.62)  LDD0851  [6]
 LDCM0254  AC135 HEK-293T C122(0.63)  LDD0852  [6]
 LDCM0255  AC136 HEK-293T C122(0.61)  LDD0853  [6]
 LDCM0256  AC137 HEK-293T C122(0.37)  LDD0854  [6]
 LDCM0257  AC138 HEK-293T C122(0.37)  LDD0855  [6]
 LDCM0258  AC139 HEK-293T C122(0.38)  LDD0856  [6]
 LDCM0259  AC14 HEK-293T C122(0.70)  LDD0857  [6]
 LDCM0260  AC140 HEK-293T C122(0.49)  LDD0858  [6]
 LDCM0261  AC141 HEK-293T C122(0.34)  LDD0859  [6]
 LDCM0262  AC142 HEK-293T C122(0.59)  LDD0860  [6]
 LDCM0270  AC15 HEK-293T C122(0.30)  LDD0868  [6]
 LDCM0278  AC19 HEK-293T C122(0.99)  LDD1517  [7]
 LDCM0282  AC22 HEK-293T C122(0.95); C258(0.93)  LDD1521  [7]
 LDCM0283  AC23 HEK-293T C122(1.01); C258(0.99)  LDD1522  [7]
 LDCM0287  AC27 HEK-293T C122(1.06)  LDD1526  [7]
 LDCM0290  AC3 HEK-293T C122(0.98)  LDD1529  [7]
 LDCM0291  AC30 HEK-293T C122(0.99); C258(0.97)  LDD1530  [7]
 LDCM0292  AC31 HEK-293T C122(0.98); C258(1.05)  LDD1531  [7]
 LDCM0296  AC35 HEK-293T C122(1.02)  LDD1535  [7]
 LDCM0299  AC38 HEK-293T C122(1.06); C258(0.99)  LDD1538  [7]
 LDCM0300  AC39 HEK-293T C122(1.11); C258(1.08)  LDD1539  [7]
 LDCM0305  AC43 HEK-293T C122(1.03)  LDD1544  [7]
 LDCM0308  AC46 HEK-293T C122(0.64)  LDD0906  [6]
 LDCM0309  AC47 HEK-293T C122(1.06)  LDD0907  [6]
 LDCM0310  AC48 HEK-293T C122(0.99)  LDD0908  [6]
 LDCM0311  AC49 HEK-293T C122(1.18)  LDD0909  [6]
 LDCM0313  AC50 HEK-293T C122(1.14)  LDD0911  [6]
 LDCM0314  AC51 HEK-293T C122(1.40)  LDD0912  [6]
 LDCM0315  AC52 HEK-293T C122(1.16)  LDD0913  [6]
 LDCM0316  AC53 HEK-293T C122(1.33)  LDD0914  [6]
 LDCM0317  AC54 HEK-293T C122(0.90)  LDD0915  [6]
 LDCM0318  AC55 HEK-293T C122(1.19)  LDD0916  [6]
 LDCM0319  AC56 HEK-293T C122(1.15)  LDD0917  [6]
 LDCM0320  AC57 HEK-293T C122(0.78)  LDD0918  [6]
 LDCM0321  AC58 HEK-293T C122(0.82)  LDD0919  [6]
 LDCM0322  AC59 HEK-293T C122(0.77)  LDD0920  [6]
 LDCM0323  AC6 HEK-293T C122(1.04)  LDD0921  [6]
 LDCM0324  AC60 HEK-293T C122(1.05)  LDD0922  [6]
 LDCM0325  AC61 HEK-293T C122(0.44)  LDD0923  [6]
 LDCM0326  AC62 HEK-293T C122(0.59)  LDD0924  [6]
 LDCM0327  AC63 HEK-293T C122(0.51)  LDD0925  [6]
 LDCM0328  AC64 HEK-293T C122(0.70)  LDD0926  [6]
 LDCM0329  AC65 HEK-293T C122(0.49)  LDD0927  [6]
 LDCM0330  AC66 HEK-293T C122(0.84)  LDD0928  [6]
 LDCM0331  AC67 HEK-293T C122(0.61)  LDD0929  [6]
 LDCM0332  AC68 HEK-293T C122(0.90)  LDD0930  [6]
 LDCM0333  AC69 HEK-293T C122(1.00)  LDD0931  [6]
 LDCM0334  AC7 HEK-293T C122(0.49)  LDD0932  [6]
 LDCM0335  AC70 HEK-293T C122(0.69)  LDD0933  [6]
 LDCM0336  AC71 HEK-293T C122(0.94)  LDD0934  [6]
 LDCM0337  AC72 HEK-293T C122(1.01)  LDD0935  [6]
 LDCM0338  AC73 HEK-293T C122(1.00)  LDD0936  [6]
 LDCM0339  AC74 HEK-293T C122(0.86)  LDD0937  [6]
 LDCM0340  AC75 HEK-293T C122(0.88)  LDD0938  [6]
 LDCM0341  AC76 HEK-293T C122(1.21)  LDD0939  [6]
 LDCM0342  AC77 HEK-293T C122(0.88)  LDD0940  [6]
 LDCM0343  AC78 HEK-293T C122(0.84)  LDD0941  [6]
 LDCM0344  AC79 HEK-293T C122(1.09)  LDD0942  [6]
 LDCM0345  AC8 HEK-293T C122(0.43)  LDD0943  [6]
 LDCM0346  AC80 HEK-293T C122(0.91)  LDD0944  [6]
 LDCM0347  AC81 HEK-293T C122(1.02)  LDD0945  [6]
 LDCM0348  AC82 HEK-293T C122(0.49)  LDD0946  [6]
 LDCM0365  AC98 HEK-293T C122(1.10)  LDD0963  [6]
 LDCM0366  AC99 HEK-293T C122(1.03)  LDD0964  [6]
 LDCM0248  AKOS034007472 HEK-293T C122(0.67)  LDD0846  [6]
 LDCM0356  AKOS034007680 HEK-293T C122(0.40)  LDD0954  [6]
 LDCM0275  AKOS034007705 HEK-293T C122(0.34)  LDD0873  [6]
 LDCM0367  CL1 HEK-293T C122(0.91)  LDD1571  [7]
 LDCM0368  CL10 HEK-293T C122(1.01); C258(1.13)  LDD1572  [7]
 LDCM0370  CL101 HEK-293T C122(0.41)  LDD0968  [6]
 LDCM0371  CL102 HEK-293T C122(0.47)  LDD0969  [6]
 LDCM0372  CL103 HEK-293T C122(0.49)  LDD0970  [6]
 LDCM0373  CL104 HEK-293T C122(0.63)  LDD0971  [6]
 LDCM0374  CL105 HEK-293T C122(1.04)  LDD1578  [7]
 LDCM0375  CL106 HEK-293T C258(0.97)  LDD1579  [7]
 LDCM0376  CL107 HEK-293T C122(1.11)  LDD1580  [7]
 LDCM0378  CL109 HEK-293T C122(1.03)  LDD1582  [7]
 LDCM0379  CL11 HEK-293T C122(1.68); C258(0.98)  LDD1583  [7]
 LDCM0380  CL110 HEK-293T C258(0.89)  LDD1584  [7]
 LDCM0381  CL111 HEK-293T C122(0.99)  LDD1585  [7]
 LDCM0383  CL113 HEK-293T C122(1.06)  LDD1587  [7]
 LDCM0384  CL114 HEK-293T C258(1.04)  LDD1588  [7]
 LDCM0385  CL115 HEK-293T C122(0.99)  LDD1589  [7]
 LDCM0387  CL117 HEK-293T C122(1.01)  LDD1591  [7]
 LDCM0388  CL118 HEK-293T C258(1.14)  LDD1592  [7]
 LDCM0389  CL119 HEK-293T C122(1.02)  LDD1593  [7]
 LDCM0392  CL121 HEK-293T C122(1.04)  LDD0990  [6]
 LDCM0393  CL122 HEK-293T C122(0.83)  LDD0991  [6]
 LDCM0394  CL123 HEK-293T C122(1.08)  LDD0992  [6]
 LDCM0395  CL124 HEK-293T C122(1.44)  LDD0993  [6]
 LDCM0396  CL125 HEK-293T C122(0.62)  LDD0994  [6]
 LDCM0397  CL126 HEK-293T C122(0.54)  LDD0995  [6]
 LDCM0398  CL127 HEK-293T C122(0.68)  LDD0996  [6]
 LDCM0399  CL128 HEK-293T C122(1.12)  LDD0997  [6]
 LDCM0400  CL13 HEK-293T C122(0.98)  LDD1604  [7]
 LDCM0401  CL14 HEK-293T C258(0.91)  LDD1605  [7]
 LDCM0402  CL15 HEK-293T C122(1.09)  LDD1606  [7]
 LDCM0406  CL19 HEK-293T C122(1.23)  LDD1610  [7]
 LDCM0407  CL2 HEK-293T C258(0.88)  LDD1611  [7]
 LDCM0410  CL22 HEK-293T C122(1.06); C258(1.17)  LDD1614  [7]
 LDCM0411  CL23 HEK-293T C122(1.86); C258(1.08)  LDD1615  [7]
 LDCM0413  CL25 HEK-293T C122(1.01)  LDD1617  [7]
 LDCM0414  CL26 HEK-293T C258(0.91)  LDD1618  [7]
 LDCM0415  CL27 HEK-293T C122(1.00)  LDD1619  [7]
 LDCM0418  CL3 HEK-293T C122(1.04)  LDD1622  [7]
 LDCM0420  CL31 HEK-293T C122(1.15)  LDD1624  [7]
 LDCM0423  CL34 HEK-293T C122(0.97); C258(1.10)  LDD1627  [7]
 LDCM0424  CL35 HEK-293T C122(2.19); C258(1.20)  LDD1628  [7]
 LDCM0426  CL37 HEK-293T C122(1.12)  LDD1630  [7]
 LDCM0428  CL39 HEK-293T C122(1.01)  LDD1632  [7]
 LDCM0433  CL43 HEK-293T C122(1.04)  LDD1637  [7]
 LDCM0436  CL46 HEK-293T C122(0.66)  LDD1034  [6]
 LDCM0437  CL47 HEK-293T C122(0.87)  LDD1035  [6]
 LDCM0438  CL48 HEK-293T C122(0.94)  LDD1036  [6]
 LDCM0439  CL49 HEK-293T C122(0.79)  LDD1037  [6]
 LDCM0441  CL50 HEK-293T C122(0.33)  LDD1039  [6]
 LDCM0442  CL51 HEK-293T C122(0.69)  LDD1040  [6]
 LDCM0443  CL52 HEK-293T C122(1.46)  LDD1041  [6]
 LDCM0444  CL53 HEK-293T C122(0.93)  LDD1042  [6]
 LDCM0445  CL54 HEK-293T C122(0.66)  LDD1043  [6]
 LDCM0446  CL55 HEK-293T C122(0.69)  LDD1044  [6]
 LDCM0447  CL56 HEK-293T C122(1.10)  LDD1045  [6]
 LDCM0448  CL57 HEK-293T C122(0.83)  LDD1046  [6]
 LDCM0449  CL58 HEK-293T C122(0.68)  LDD1047  [6]
 LDCM0450  CL59 HEK-293T C122(0.52)  LDD1048  [6]
 LDCM0452  CL60 HEK-293T C122(0.34)  LDD1050  [6]
 LDCM0453  CL61 HEK-293T C122(0.96)  LDD1656  [7]
 LDCM0454  CL62 HEK-293T C258(1.01)  LDD1657  [7]
 LDCM0455  CL63 HEK-293T C122(1.15)  LDD1658  [7]
 LDCM0459  CL67 HEK-293T C122(1.11)  LDD1662  [7]
 LDCM0462  CL7 HEK-293T C122(1.10)  LDD1665  [7]
 LDCM0463  CL70 HEK-293T C122(1.01); C258(1.08)  LDD1666  [7]
 LDCM0464  CL71 HEK-293T C122(1.83); C258(1.20)  LDD1667  [7]
 LDCM0466  CL73 HEK-293T C122(1.08)  LDD1669  [7]
 LDCM0467  CL74 HEK-293T C258(0.95)  LDD1670  [7]
 LDCM0472  CL79 HEK-293T C122(1.13)  LDD1675  [7]
 LDCM0476  CL82 HEK-293T C122(1.08); C258(1.07)  LDD1679  [7]
 LDCM0477  CL83 HEK-293T C122(1.88); C258(1.12)  LDD1680  [7]
 LDCM0479  CL85 HEK-293T C122(0.97)  LDD1682  [7]
 LDCM0480  CL86 HEK-293T C258(0.99)  LDD1683  [7]
 LDCM0481  CL87 HEK-293T C122(0.90)  LDD1684  [7]
 LDCM0486  CL91 HEK-293T C122(1.11)  LDD1689  [7]
 LDCM0489  CL94 HEK-293T C122(1.03); C258(1.11)  LDD1692  [7]
 LDCM0490  CL95 HEK-293T C122(1.72); C258(1.09)  LDD1693  [7]
 LDCM0492  CL97 HEK-293T C122(1.04)  LDD1695  [7]
 LDCM0493  CL98 HEK-293T C258(1.03)  LDD1696  [7]
 LDCM0494  CL99 HEK-293T C122(1.05)  LDD1697  [7]
 LDCM0495  E2913 HEK-293T C122(0.97)  LDD1698  [7]
 LDCM0468  Fragment33 HEK-293T C122(1.08)  LDD1671  [7]
 LDCM0427  Fragment51 HEK-293T C258(0.95)  LDD1631  [7]
 LDCM0022  KB02 A2058 C57(2.02)  LDD2253  [1]
 LDCM0023  KB03 A2058 C57(2.15)  LDD2670  [1]
 LDCM0024  KB05 TE-8 C74(3.15)  LDD3470  [1]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Histone deacetylase 1 (HDAC1) Histone deacetylase family Q13547

References

1 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
2 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
3 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
4 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.
5 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
6 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
7 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402