Details of the Target

General Information of Target

Target ID LDTP06095
Target Name Endonuclease G, mitochondrial (ENDOG)
Gene Name ENDOG
Gene ID 2021
Synonyms
Endonuclease G, mitochondrial; Endo G; EC 3.1.30.-
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MRALRAGLTLASGAGLGAVVEGWRRRREDARAAPGLLGRLPVLPVAAAAELPPVPGGPRG
PGELAKYGLPGLAQLKSRESYVLCYDPRTRGALWVVEQLRPERLRGDGDRRECDFREDDS
VHAYHRATNADYRGSGFDRGHLAAAANHRWSQKAMDDTFYLSNVAPQVPHLNQNAWNNLE
KYSRSLTRSYQNVYVCTGPLFLPRTEADGKSYVKYQVIGKNHVAVPTHFFKVLILEAAGG
QIELRTYVMPNAPVDEAIPLERFLVPIESIERASGLLFVPNILARAGSLKAITAGSK
Target Bioclass
Enzyme
Family
DNA/RNA non-specific endonuclease family
Subcellular location
Mitochondrion
Function
Endonuclease that preferentially catalyzes the cleavage of double-stranded 5-hydroxymethylcytosine (5hmC)-modified DNA. The 5hmC-modified nucleotide does not increase the binding affinity, but instead increases the efficiency of cutting and specifies the site of cleavage for the modified DNAs. Shows significantly higher affinity for four-stranded Holliday junction over duplex and single-stranded DNAs. Promotes conservative recombination when the DNA is 5hmC-modified. Promotes autophagy through the suppression of mTOR by its phosphorylation-mediated interaction with YWHAG and its endonuclease activity-mediated DNA damage response. GSK3-beta mediated phosphorylation of ENDOG enhances its interaction with YWHAG, leading to the release of TSC2 and PIK3C3 from YWHAG resulting in mTOR pathway suppression and autophagy initiation. Promotes cleavage of mtDNA in response to oxidative and nitrosative stress, in turn inducing compensatory mtDNA replication.
Uniprot ID
Q14249
Ensemble ID
ENST00000372642.5
HGNC ID
HGNC:3346
ChEMBL ID
CHEMBL3804749

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 8 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
STPyne
 Probe Info  		K214(3.69)  LDD0277  [2]
IPM
 Probe Info  		N.A.  LDD0241  [3]
DBIA
 Probe Info  		C196(1.19)  LDD0531  [4]
m-APA
 Probe Info  		N.A.  LDD2232  [5]
IA-alkyne
 Probe Info  		N.A.  LDD0165  [6]
TFBX
 Probe Info  		N.A.  LDD0027  [7]
Acrolein
 Probe Info  		N.A.  LDD0217  [8]
NAIA_5
 Probe Info  		C113(0.00); C196(0.00)  LDD2223  [9]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0214  AC1 HCT 116 C196(1.19)  LDD0531  [4]
 LDCM0216  AC100 HCT 116 C196(0.53)  LDD0533  [4]
 LDCM0217  AC101 HCT 116 C196(0.68)  LDD0534  [4]
 LDCM0218  AC102 HCT 116 C196(1.08)  LDD0535  [4]
 LDCM0219  AC103 HCT 116 C196(0.52)  LDD0536  [4]
 LDCM0220  AC104 HCT 116 C196(0.83)  LDD0537  [4]
 LDCM0221  AC105 HCT 116 C196(0.60)  LDD0538  [4]
 LDCM0222  AC106 HCT 116 C196(0.47)  LDD0539  [4]
 LDCM0223  AC107 HCT 116 C196(0.57)  LDD0540  [4]
 LDCM0224  AC108 HCT 116 C196(0.56)  LDD0541  [4]
 LDCM0225  AC109 HCT 116 C196(0.82)  LDD0542  [4]
 LDCM0227  AC110 HCT 116 C196(0.59)  LDD0544  [4]
 LDCM0228  AC111 HCT 116 C196(0.58)  LDD0545  [4]
 LDCM0229  AC112 HCT 116 C196(0.63)  LDD0546  [4]
 LDCM0279  AC2 HCT 116 C196(0.84)  LDD0596  [4]
 LDCM0290  AC3 HCT 116 C196(1.05)  LDD0607  [4]
 LDCM0296  AC35 HCT 116 C196(1.25)  LDD0613  [4]
 LDCM0297  AC36 HCT 116 C196(0.86)  LDD0614  [4]
 LDCM0298  AC37 HCT 116 C196(0.71)  LDD0615  [4]
 LDCM0299  AC38 HCT 116 C196(0.95)  LDD0616  [4]
 LDCM0300  AC39 HCT 116 C196(1.37)  LDD0617  [4]
 LDCM0301  AC4 HCT 116 C196(1.25)  LDD0618  [4]
 LDCM0302  AC40 HCT 116 C196(1.08)  LDD0619  [4]
 LDCM0303  AC41 HCT 116 C196(0.56)  LDD0620  [4]
 LDCM0304  AC42 HCT 116 C196(0.58)  LDD0621  [4]
 LDCM0305  AC43 HCT 116 C196(0.68)  LDD0622  [4]
 LDCM0306  AC44 HCT 116 C196(0.70)  LDD0623  [4]
 LDCM0307  AC45 HCT 116 C196(0.69)  LDD0624  [4]
 LDCM0312  AC5 HCT 116 C196(1.06)  LDD0629  [4]
 LDCM0365  AC98 HCT 116 C196(0.50)  LDD0682  [4]
 LDCM0366  AC99 HCT 116 C196(0.60)  LDD0683  [4]
 LDCM0369  CL100 HCT 116 C196(1.15)  LDD0686  [4]
 LDCM0387  CL117 HCT 116 C196(0.77)  LDD0704  [4]
 LDCM0388  CL118 HCT 116 C196(0.79)  LDD0705  [4]
 LDCM0389  CL119 HCT 116 C196(0.76)  LDD0706  [4]
 LDCM0391  CL120 HCT 116 C196(0.81)  LDD0708  [4]
 LDCM0403  CL16 HCT 116 C196(1.01)  LDD0720  [4]
 LDCM0404  CL17 HCT 116 C196(0.68)  LDD0721  [4]
 LDCM0405  CL18 HCT 116 C196(0.63)  LDD0722  [4]
 LDCM0406  CL19 HCT 116 C196(0.85)  LDD0723  [4]
 LDCM0408  CL20 HCT 116 C196(0.97)  LDD0725  [4]
 LDCM0409  CL21 HCT 116 C196(0.77)  LDD0726  [4]
 LDCM0410  CL22 HCT 116 C196(0.43)  LDD0727  [4]
 LDCM0411  CL23 HCT 116 C196(0.74)  LDD0728  [4]
 LDCM0412  CL24 HCT 116 C196(0.75)  LDD0729  [4]
 LDCM0413  CL25 HCT 116 C196(0.85)  LDD0730  [4]
 LDCM0414  CL26 HCT 116 C196(0.70)  LDD0731  [4]
 LDCM0415  CL27 HCT 116 C196(0.93)  LDD0732  [4]
 LDCM0416  CL28 HCT 116 C196(0.72)  LDD0733  [4]
 LDCM0417  CL29 HCT 116 C196(0.92)  LDD0734  [4]
 LDCM0419  CL30 HCT 116 C196(0.83)  LDD0736  [4]
 LDCM0420  CL31 HCT 116 C196(1.00)  LDD0737  [4]
 LDCM0421  CL32 HCT 116 C196(0.88)  LDD0738  [4]
 LDCM0422  CL33 HCT 116 C196(0.88)  LDD0739  [4]
 LDCM0423  CL34 HCT 116 C196(0.98)  LDD0740  [4]
 LDCM0424  CL35 HCT 116 C196(0.86)  LDD0741  [4]
 LDCM0425  CL36 HCT 116 C196(1.26)  LDD0742  [4]
 LDCM0426  CL37 HCT 116 C196(0.85)  LDD0743  [4]
 LDCM0428  CL39 HCT 116 C196(1.03)  LDD0745  [4]
 LDCM0430  CL40 HCT 116 C196(0.94)  LDD0747  [4]
 LDCM0431  CL41 HCT 116 C196(0.90)  LDD0748  [4]
 LDCM0432  CL42 HCT 116 C196(0.54)  LDD0749  [4]
 LDCM0433  CL43 HCT 116 C196(0.75)  LDD0750  [4]
 LDCM0434  CL44 HCT 116 C196(0.88)  LDD0751  [4]
 LDCM0435  CL45 HCT 116 C196(0.65)  LDD0752  [4]
 LDCM0486  CL91 HCT 116 C196(0.64)  LDD0803  [4]
 LDCM0487  CL92 HCT 116 C196(0.93)  LDD0804  [4]
 LDCM0488  CL93 HCT 116 C196(1.08)  LDD0805  [4]
 LDCM0489  CL94 HCT 116 C196(1.53)  LDD0806  [4]
 LDCM0490  CL95 HCT 116 C196(0.88)  LDD0807  [4]
 LDCM0491  CL96 HCT 116 C196(0.93)  LDD0808  [4]
 LDCM0492  CL97 HCT 116 C196(0.74)  LDD0809  [4]
 LDCM0493  CL98 HCT 116 C196(0.93)  LDD0810  [4]
 LDCM0494  CL99 HCT 116 C196(0.98)  LDD0811  [4]
 LDCM0572  Fragment10 Ramos C196(1.07)  LDD2189  [10]
 LDCM0573  Fragment11 Ramos C196(0.43)  LDD2190  [10]
 LDCM0574  Fragment12 Ramos C196(0.93)  LDD2191  [10]
 LDCM0575  Fragment13 Ramos C196(1.01)  LDD2192  [10]
 LDCM0576  Fragment14 Ramos C196(2.24)  LDD2193  [10]
 LDCM0579  Fragment20 Ramos C196(0.66)  LDD2194  [10]
 LDCM0580  Fragment21 Ramos C196(0.72)  LDD2195  [10]
 LDCM0582  Fragment23 Ramos C196(1.57)  LDD2196  [10]
 LDCM0578  Fragment27 Ramos C196(0.92)  LDD2197  [10]
 LDCM0588  Fragment30 Ramos C196(0.66)  LDD2199  [10]
 LDCM0589  Fragment31 Ramos C196(1.38)  LDD2200  [10]
 LDCM0590  Fragment32 Ramos C196(0.75)  LDD2201  [10]
 LDCM0468  Fragment33 Ramos C196(0.48)  LDD2202  [10]
 LDCM0596  Fragment38 Ramos C196(1.32)  LDD2203  [10]
 LDCM0427  Fragment51 HCT 116 C196(0.62)  LDD0744  [4]
 LDCM0610  Fragment52 Ramos C196(0.87)  LDD2204  [10]
 LDCM0614  Fragment56 Ramos C196(0.68)  LDD2205  [10]
 LDCM0571  Fragment9 Ramos C196(1.35)  LDD2188  [10]
 LDCM0022  KB02 786-O C84(1.44)  LDD2247  [11]
 LDCM0023  KB03 786-O C84(1.79)  LDD2664  [11]
 LDCM0024  KB05 HMCB C84(1.28)  LDD3312  [11]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Other
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
DnaJ homolog subfamily A member 4 (DNAJA4) . Q8WW22

References

1 Labeling Preferences of Diazirines with Protein Biomolecules. J Am Chem Soc. 2021 May 5;143(17):6691-6700. doi: 10.1021/jacs.1c02509. Epub 2021 Apr 20.
Mass spectrometry data entry: PXD025140
2 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
3 Oxidant-Induced Bioconjugation for Protein Labeling in Live Cells. ACS Chem Biol. 2023 Jan 20;18(1):112-122. doi: 10.1021/acschembio.2c00740. Epub 2022 Dec 21.
4 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
5 Global profiling of functional histidines in live cells using small-molecule photosensitizer and chemical probe relay labelling. Nat Chem. 2024 Jun 4. doi: 10.1038/s41557-024-01545-6. Online ahead of print.
Mass spectrometry data entry: PXD042377
6 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
7 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
8 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
9 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
10 Site-specific quantitative cysteine profiling with data-independent acquisition-based mass spectrometry. Methods Enzymol. 2023;679:295-322. doi: 10.1016/bs.mie.2022.07.037. Epub 2022 Sep 7.
Mass spectrometry data entry: PXD027578
11 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840