Details of the Target

General Information of Target

Target ID LDTP05580
Target Name Protein spire homolog 1 (SPIRE1)
Gene Name SPIRE1
Gene ID 56907
Synonyms
KIAA1135; SPIR1; Protein spire homolog 1; Spir-1
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MAQAAGPAGGGEPRTEAVGGEGPREPGAAGGAAGGSRDALSLEEILRLYNQPINEEQAWA
VCYQCCGSLRAAARRRQPRHRVRSAAQIRVWRDGAVTLAPAADDAGEPPPVAGKLGYSQC
METEVIESLGIIIYKALDYGLKENEERELSPPLEQLIDHMANTVEADGSNDEGYEAAEEG
LGDEDEKRKISAIRSYRDVMKLCAAHLPTESDAPNHYQAVCRALFAETMELHTFLTKIKS
AKENLKKIQEMEKSDESSTDLEELKNADWARFWVQVMRDLRNGVKLKKVQERQYNPLPIE
YQLTPYEMLMDDIRCKRYTLRKVMVNGDIPPRLKKSAHEIILDFIRSRPPLNPVSARKLK
PTPPRPRSLHERILEEIKAERKLRPVSPEEIRRSRLAMRPLSMSYSFDLSDVTTPESTKN
LVESSMVNGGLTSQTKENGLSTSQQVPAQRKKLLRAPTLAELDSSESEEETLHKSTSSSS
VSPSFPEEPVLEAVSTRKKPPKFLPISSTPQPERRQPPQRRHSIEKETPTNVRQFLPPSR
QSSRSLEEFCYPVECLALTVEEVMHIRQVLVKAELEKYQQYKDIYTALKKGKLCFCCRTR
RFSFFTWSYTCQFCKRPVCSQCCKKMRLPSKPYSTLPIFSLGPSALQRGESSMRSEKPST
AHHRPLRSIARFSSKSKSMDKSDEELQFPKELMEDWSTMEVCVDCKKFISEIISSSRRSL
VLANKRARLKRKTQSFYMSSPGPSEYCPSERTISEI
Target Bioclass
Other
Family
Spire family
Subcellular location
Cytoplasm, cytoskeleton
Function
Acts as an actin nucleation factor, remains associated with the slow-growing pointed end of the new filament. Involved in intracellular vesicle transport along actin fibers, providing a novel link between actin cytoskeleton dynamics and intracellular transport. Required for asymmetric spindle positioning and asymmetric cell division during meiosis. Required for normal formation of the cleavage furrow and for polar body extrusion during female germ cell meiosis. Also acts in the nucleus: together with FMN2, promotes assembly of nuclear actin filaments in response to DNA damage in order to facilitate movement of chromatin and repair factors after DNA damage. In addition, promotes innate immune signaling downstream of dsRNA sensing. Mechanistically, contributes to IRF3 phosphorylation and activation downstream of MAVS and upstream of TBK1.
Uniprot ID
Q08AE8
Ensemble ID
ENST00000409402.9
HGNC ID
HGNC:30622

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
CAL120 SNV: p.S36T DBIA    Probe Info 
CAMA1 SNV: p.K246N; p.E256K .
HCT15 SNV: p.D411Y .
HEC1 SNV: p.R292W .
HEC1B SNV: p.R292W .
JHH7 SNV: p.E124Ter .
KMCH1 SNV: p.W269C .
L428 SNV: p.E554K .
MCAS SNV: p.R194L .
SISO SNV: p.Q434R .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 4 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
STPyne
 Probe Info  		K725(10.00)  LDD0277  [1]
DBIA
 Probe Info  		C747(1.63)  LDD3406  [2]
AHL-Pu-1
 Probe Info  		C747(2.09)  LDD0169  [3]
NAIA_4
 Probe Info  		N.A.  LDD2226  [4]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0026  4SU-RNA+native RNA HEK-293T C747(2.09)  LDD0169  [3]
 LDCM0214  AC1 PaTu 8988t C619(0.27); C622(0.27); C623(0.27)  LDD1093  [5]
 LDCM0215  AC10 HEK-293T C747(1.27)  LDD1508  [6]
 LDCM0226  AC11 HEK-293T C747(0.79)  LDD1509  [6]
 LDCM0237  AC12 HEK-293T C747(0.91)  LDD1510  [6]
 LDCM0263  AC143 PaTu 8988t C619(0.81); C622(0.81); C623(0.81)  LDD1142  [5]
 LDCM0264  AC144 PaTu 8988t C619(0.72); C622(0.72); C623(0.72)  LDD1143  [5]
 LDCM0265  AC145 PaTu 8988t C619(0.62); C622(0.62); C623(0.62)  LDD1144  [5]
 LDCM0266  AC146 PaTu 8988t C619(0.65); C622(0.65); C623(0.65)  LDD1145  [5]
 LDCM0267  AC147 PaTu 8988t C619(0.66); C622(0.66); C623(0.66)  LDD1146  [5]
 LDCM0268  AC148 PaTu 8988t C619(0.76); C622(0.76); C623(0.76)  LDD1147  [5]
 LDCM0269  AC149 PaTu 8988t C619(0.82); C622(0.82); C623(0.82)  LDD1148  [5]
 LDCM0271  AC150 PaTu 8988t C619(0.92); C622(0.92); C623(0.92)  LDD1150  [5]
 LDCM0272  AC151 PaTu 8988t C619(0.82); C622(0.82); C623(0.82)  LDD1151  [5]
 LDCM0273  AC152 PaTu 8988t C619(1.05); C622(1.05); C623(1.05)  LDD1152  [5]
 LDCM0274  AC153 PaTu 8988t C619(0.92); C622(0.92); C623(0.92)  LDD1153  [5]
 LDCM0621  AC154 PaTu 8988t C619(1.00); C622(1.00); C623(1.00)  LDD2166  [5]
 LDCM0622  AC155 PaTu 8988t C619(0.63); C622(0.63); C623(0.63)  LDD2167  [5]
 LDCM0623  AC156 PaTu 8988t C619(0.78); C622(0.78); C623(0.78)  LDD2168  [5]
 LDCM0624  AC157 PaTu 8988t C619(1.27); C622(1.27); C623(1.27)  LDD2169  [5]
 LDCM0277  AC18 HEK-293T C747(1.47)  LDD1516  [6]
 LDCM0278  AC19 HEK-293T C747(0.93)  LDD1517  [6]
 LDCM0279  AC2 PaTu 8988t C619(0.23); C622(0.23); C623(0.23)  LDD1158  [5]
 LDCM0280  AC20 HEK-293T C747(0.85)  LDD1519  [6]
 LDCM0284  AC24 HEK-293T C747(0.85)  LDD1523  [6]
 LDCM0286  AC26 HEK-293T C747(1.08)  LDD1525  [6]
 LDCM0287  AC27 HEK-293T C747(1.11)  LDD1526  [6]
 LDCM0288  AC28 HEK-293T C747(0.92)  LDD1527  [6]
 LDCM0290  AC3 PaTu 8988t C619(0.27); C622(0.27); C623(0.27)  LDD1169  [5]
 LDCM0293  AC32 HEK-293T C747(0.94)  LDD1532  [6]
 LDCM0295  AC34 HEK-293T C747(0.87)  LDD1534  [6]
 LDCM0296  AC35 PaTu 8988t C619(1.50); C622(1.50); C623(1.50)  LDD1175  [5]
 LDCM0297  AC36 PaTu 8988t C619(1.32); C622(1.32); C623(1.32)  LDD1176  [5]
 LDCM0298  AC37 PaTu 8988t C619(0.91); C622(0.91); C623(0.91)  LDD1177  [5]
 LDCM0299  AC38 PaTu 8988t C619(0.79); C622(0.79); C623(0.79)  LDD1178  [5]
 LDCM0300  AC39 PaTu 8988t C619(0.93); C622(0.93); C623(0.93)  LDD1179  [5]
 LDCM0301  AC4 PaTu 8988t C619(0.32); C622(0.32); C623(0.32)  LDD1180  [5]
 LDCM0302  AC40 PaTu 8988t C619(1.18); C622(1.18); C623(1.18)  LDD1181  [5]
 LDCM0303  AC41 PaTu 8988t C619(0.88); C622(0.88); C623(0.88)  LDD1182  [5]
 LDCM0304  AC42 PaTu 8988t C619(0.75); C622(0.75); C623(0.75)  LDD1183  [5]
 LDCM0305  AC43 PaTu 8988t C619(0.99); C622(0.99); C623(0.99)  LDD1184  [5]
 LDCM0306  AC44 PaTu 8988t C619(1.23); C622(1.23); C623(1.23)  LDD1185  [5]
 LDCM0307  AC45 PaTu 8988t C619(0.93); C622(0.93); C623(0.93)  LDD1186  [5]
 LDCM0308  AC46 PaTu 8988t C619(0.77); C622(0.77); C623(0.77)  LDD1187  [5]
 LDCM0309  AC47 PaTu 8988t C619(0.91); C622(0.91); C623(0.91)  LDD1188  [5]
 LDCM0310  AC48 PaTu 8988t C619(0.59); C622(0.59); C623(0.59)  LDD1189  [5]
 LDCM0311  AC49 PaTu 8988t C619(0.90); C622(0.90); C623(0.90)  LDD1190  [5]
 LDCM0312  AC5 PaTu 8988t C619(0.32); C622(0.32); C623(0.32)  LDD1191  [5]
 LDCM0313  AC50 PaTu 8988t C619(0.86); C622(0.86); C623(0.86)  LDD1192  [5]
 LDCM0314  AC51 PaTu 8988t C619(0.66); C622(0.66); C623(0.66)  LDD1193  [5]
 LDCM0315  AC52 PaTu 8988t C619(1.03); C622(1.03); C623(1.03)  LDD1194  [5]
 LDCM0316  AC53 PaTu 8988t C619(0.72); C622(0.72); C623(0.72)  LDD1195  [5]
 LDCM0317  AC54 PaTu 8988t C619(0.87); C622(0.87); C623(0.87)  LDD1196  [5]
 LDCM0318  AC55 PaTu 8988t C619(0.81); C622(0.81); C623(0.81)  LDD1197  [5]
 LDCM0319  AC56 PaTu 8988t C619(0.84); C622(0.84); C623(0.84)  LDD1198  [5]
 LDCM0321  AC58 HEK-293T C747(0.88)  LDD1560  [6]
 LDCM0322  AC59 HEK-293T C747(0.91)  LDD1561  [6]
 LDCM0324  AC60 HEK-293T C747(0.85)  LDD1563  [6]
 LDCM0328  AC64 HEK-293T C747(1.12)  LDD1567  [6]
 LDCM0332  AC68 PaTu 8988t C619(0.99); C622(0.99); C623(0.99)  LDD1211  [5]
 LDCM0333  AC69 PaTu 8988t C619(0.83); C622(0.83); C623(0.83)  LDD1212  [5]
 LDCM0335  AC70 PaTu 8988t C619(1.26); C622(1.26); C623(1.26)  LDD1214  [5]
 LDCM0336  AC71 PaTu 8988t C619(0.91); C622(0.91); C623(0.91)  LDD1215  [5]
 LDCM0337  AC72 PaTu 8988t C619(0.79); C622(0.79); C623(0.79)  LDD1216  [5]
 LDCM0338  AC73 PaTu 8988t C619(1.15); C622(1.15); C623(1.15)  LDD1217  [5]
 LDCM0339  AC74 PaTu 8988t C619(1.13); C622(1.13); C623(1.13)  LDD1218  [5]
 LDCM0340  AC75 PaTu 8988t C619(0.88); C622(0.88); C623(0.88)  LDD1219  [5]
 LDCM0341  AC76 PaTu 8988t C619(0.74); C622(0.74); C623(0.74)  LDD1220  [5]
 LDCM0342  AC77 PaTu 8988t C619(1.04); C622(1.04); C623(1.04)  LDD1221  [5]
 LDCM0343  AC78 PaTu 8988t C619(1.06); C622(1.06); C623(1.06)  LDD1222  [5]
 LDCM0344  AC79 PaTu 8988t C619(0.96); C622(0.96); C623(0.96)  LDD1223  [5]
 LDCM0345  AC8 HEK-293T C747(0.97)  LDD1569  [6]
 LDCM0346  AC80 PaTu 8988t C619(0.76); C622(0.76); C623(0.76)  LDD1225  [5]
 LDCM0347  AC81 PaTu 8988t C619(0.94); C622(0.94); C623(0.94)  LDD1226  [5]
 LDCM0348  AC82 PaTu 8988t C619(1.02); C622(1.02); C623(1.02)  LDD1227  [5]
 LDCM0275  AKOS034007705 HEK-293T C747(0.98)  LDD1514  [6]
 LDCM0367  CL1 HEK-293T C747(1.15)  LDD1571  [6]
 LDCM0369  CL100 PaTu 8988t C619(0.28); C622(0.28); C623(0.28)  LDD1248  [5]
 LDCM0370  CL101 HEK-293T C747(0.87)  LDD1574  [6]
 LDCM0374  CL105 HEK-293T C747(1.08)  LDD1578  [6]
 LDCM0378  CL109 HEK-293T C747(0.72)  LDD1582  [6]
 LDCM0383  CL113 HEK-293T C747(1.00)  LDD1587  [6]
 LDCM0387  CL117 PaTu 8988t C619(1.03); C622(1.03); C623(1.03)  LDD1266  [5]
 LDCM0388  CL118 PaTu 8988t C619(0.90); C622(0.90); C623(0.90)  LDD1267  [5]
 LDCM0389  CL119 PaTu 8988t C619(2.02); C622(2.02); C623(2.02)  LDD1268  [5]
 LDCM0390  CL12 HEK-293T C747(1.04)  LDD1594  [6]
 LDCM0391  CL120 PaTu 8988t C619(1.06); C622(1.06); C623(1.06)  LDD1270  [5]
 LDCM0392  CL121 PaTu 8988t C619(0.64); C622(0.64); C623(0.64)  LDD1271  [5]
 LDCM0393  CL122 PaTu 8988t C619(0.96); C622(0.96); C623(0.96)  LDD1272  [5]
 LDCM0394  CL123 PaTu 8988t C619(0.99); C622(0.99); C623(0.99)  LDD1273  [5]
 LDCM0395  CL124 PaTu 8988t C619(0.92); C622(0.92); C623(0.92)  LDD1274  [5]
 LDCM0396  CL125 HEK-293T C747(0.72)  LDD1600  [6]
 LDCM0400  CL13 HEK-293T C747(0.97)  LDD1604  [6]
 LDCM0405  CL18 HEK-293T C747(1.25)  LDD1609  [6]
 LDCM0406  CL19 HEK-293T C747(0.94)  LDD1610  [6]
 LDCM0408  CL20 HEK-293T C747(1.18)  LDD1612  [6]
 LDCM0412  CL24 HEK-293T C747(1.10)  LDD1616  [6]
 LDCM0413  CL25 HEK-293T C747(0.90)  LDD1617  [6]
 LDCM0419  CL30 HEK-293T C747(1.04)  LDD1623  [6]
 LDCM0420  CL31 PaTu 8988t C619(1.43); C622(1.43); C623(1.43)  LDD1299  [5]
 LDCM0421  CL32 PaTu 8988t C619(1.09); C622(1.09); C623(1.09)  LDD1300  [5]
 LDCM0422  CL33 PaTu 8988t C619(0.90); C622(0.90); C623(0.90)  LDD1301  [5]
 LDCM0423  CL34 PaTu 8988t C619(1.20); C622(1.20); C623(1.20)  LDD1302  [5]
 LDCM0424  CL35 PaTu 8988t C619(0.74); C622(0.74); C623(0.74)  LDD1303  [5]
 LDCM0425  CL36 PaTu 8988t C619(0.36); C622(0.36); C623(0.36)  LDD1304  [5]
 LDCM0426  CL37 PaTu 8988t C619(0.99); C622(0.99); C623(0.99)  LDD1305  [5]
 LDCM0428  CL39 PaTu 8988t C619(0.91); C622(0.91); C623(0.91)  LDD1307  [5]
 LDCM0430  CL40 PaTu 8988t C619(1.03); C622(1.03); C623(1.03)  LDD1309  [5]
 LDCM0431  CL41 PaTu 8988t C619(0.94); C622(0.94); C623(0.94)  LDD1310  [5]
 LDCM0432  CL42 PaTu 8988t C619(1.33); C622(1.33); C623(1.33)  LDD1311  [5]
 LDCM0433  CL43 PaTu 8988t C619(1.33); C622(1.33); C623(1.33)  LDD1312  [5]
 LDCM0434  CL44 PaTu 8988t C619(0.82); C622(0.82); C623(0.82)  LDD1313  [5]
 LDCM0435  CL45 PaTu 8988t C619(0.97); C622(0.97); C623(0.97)  LDD1314  [5]
 LDCM0438  CL48 HEK-293T C747(0.99)  LDD1642  [6]
 LDCM0439  CL49 HEK-293T C747(0.91)  LDD1643  [6]
 LDCM0445  CL54 HEK-293T C747(1.11)  LDD1648  [6]
 LDCM0446  CL55 HEK-293T C747(1.11)  LDD1649  [6]
 LDCM0447  CL56 HEK-293T C747(0.85)  LDD1650  [6]
 LDCM0451  CL6 HEK-293T C747(0.89)  LDD1654  [6]
 LDCM0452  CL60 HEK-293T C747(1.07)  LDD1655  [6]
 LDCM0453  CL61 HEK-293T C747(1.03)  LDD1656  [6]
 LDCM0458  CL66 HEK-293T C747(0.99)  LDD1661  [6]
 LDCM0459  CL67 HEK-293T C747(0.86)  LDD1662  [6]
 LDCM0460  CL68 HEK-293T C747(1.02)  LDD1663  [6]
 LDCM0462  CL7 HEK-293T C747(0.72)  LDD1665  [6]
 LDCM0465  CL72 HEK-293T C747(1.41)  LDD1668  [6]
 LDCM0466  CL73 HEK-293T C747(1.16)  LDD1669  [6]
 LDCM0469  CL76 PaTu 8988t C619(1.22); C622(1.22); C623(1.22)  LDD1348  [5]
 LDCM0470  CL77 PaTu 8988t C619(0.89); C622(0.89); C623(0.89)  LDD1349  [5]
 LDCM0471  CL78 PaTu 8988t C619(1.41); C622(1.41); C623(1.41)  LDD1350  [5]
 LDCM0472  CL79 PaTu 8988t C619(0.94); C622(0.94); C623(0.94)  LDD1351  [5]
 LDCM0473  CL8 HEK-293T C747(0.85)  LDD1676  [6]
 LDCM0474  CL80 PaTu 8988t C619(1.07); C622(1.07); C623(1.07)  LDD1353  [5]
 LDCM0475  CL81 PaTu 8988t C619(1.31); C622(1.31); C623(1.31)  LDD1354  [5]
 LDCM0476  CL82 PaTu 8988t C619(1.28); C622(1.28); C623(1.28)  LDD1355  [5]
 LDCM0477  CL83 PaTu 8988t C619(1.14); C622(1.14); C623(1.14)  LDD1356  [5]
 LDCM0478  CL84 PaTu 8988t C619(1.18); C622(1.18); C623(1.18)  LDD1357  [5]
 LDCM0479  CL85 PaTu 8988t C619(1.27); C622(1.27); C623(1.27)  LDD1358  [5]
 LDCM0480  CL86 PaTu 8988t C619(1.15); C622(1.15); C623(1.15)  LDD1359  [5]
 LDCM0481  CL87 PaTu 8988t C619(0.90); C622(0.90); C623(0.90)  LDD1360  [5]
 LDCM0482  CL88 PaTu 8988t C619(1.64); C622(1.64); C623(1.64)  LDD1361  [5]
 LDCM0483  CL89 PaTu 8988t C619(1.12); C622(1.12); C623(1.12)  LDD1362  [5]
 LDCM0485  CL90 PaTu 8988t C619(1.03); C622(1.03); C623(1.03)  LDD1364  [5]
 LDCM0486  CL91 PaTu 8988t C619(0.33); C622(0.33); C623(0.33)  LDD1365  [5]
 LDCM0487  CL92 PaTu 8988t C619(0.28); C622(0.28); C623(0.28)  LDD1366  [5]
 LDCM0488  CL93 PaTu 8988t C619(0.24); C622(0.24); C623(0.24)  LDD1367  [5]
 LDCM0489  CL94 PaTu 8988t C619(0.29); C622(0.29); C623(0.29)  LDD1368  [5]
 LDCM0490  CL95 PaTu 8988t C619(0.25); C622(0.25); C623(0.25)  LDD1369  [5]
 LDCM0491  CL96 PaTu 8988t C619(0.25); C622(0.25); C623(0.25)  LDD1370  [5]
 LDCM0492  CL97 PaTu 8988t C619(0.32); C622(0.32); C623(0.32)  LDD1371  [5]
 LDCM0493  CL98 PaTu 8988t C619(0.23); C622(0.23); C623(0.23)  LDD1372  [5]
 LDCM0494  CL99 PaTu 8988t C619(0.35); C622(0.35); C623(0.35)  LDD1373  [5]
 LDCM0427  Fragment51 PaTu 8988t C619(1.76); C622(1.76); C623(1.76)  LDD1306  [5]
 LDCM0022  KB02 CAL-120 C747(1.53)  LDD2284  [2]
 LDCM0023  KB03 CAL-120 C747(2.48)  LDD2701  [2]
 LDCM0024  KB05 RKN C747(1.63)  LDD3406  [2]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Sodium/potassium-transporting ATPase subunit alpha-3 (ATP1A3) Cation transport ATPase (P-type) (TC 3.A.3) family P13637
Phospholipase D1 (PLD1) Phospholipase D family Q13393
Transporter and channel
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Bardet-Biedl syndrome 4 protein (BBS4) BBS4 family Q96RK4
Immunoglobulin
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Platelet endothelial cell adhesion molecule (PECAM1) . P16284

References

1 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
2 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
3 Chemoproteomic capture of RNA binding activity in living cells. Nat Commun. 2023 Oct 7;14(1):6282. doi: 10.1038/s41467-023-41844-z.
Mass spectrometry data entry: PXD044625
4 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
5 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
6 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402