Details of the Target

General Information of Target

Target ID LDTP05497
Target Name DNA repair protein RAD51 homolog 1 (RAD51)
Gene Name RAD51
Gene ID 5888
Synonyms
RAD51A; RECA; DNA repair protein RAD51 homolog 1; HsRAD51; hRAD51; RAD51 homolog A
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
Download
Sequence
MAMQMQLEANADTSVEEESFGPQPISRLEQCGINANDVKKLEEAGFHTVEAVAYAPKKEL
INIKGISEAKADKILAEAAKLVPMGFTTATEFHQRRSEIIQITTGSKELDKLLQGGIETG
SITEMFGEFRTGKTQICHTLAVTCQLPIDRGGGEGKAMYIDTEGTFRPERLLAVAERYGL
SGSDVLDNVAYARAFNTDHQTQLLYQASAMMVESRYALLIVDSATALYRTDYSGRGELSA
RQMHLARFLRMLLRLADEFGVAVVITNQVVAQVDGAAMFAADPKKPIGGNIIAHASTTRL
YLRKGRGETRICKIYDSPCLPEAEAMFAINADGVGDAKD
Target Type
Clinical trial
Target Bioclass
Other
Family
RecA family, RAD51 subfamily
Subcellular location
Nucleus
Function
Plays an important role in homologous strand exchange, a key step in DNA repair through homologous recombination (HR) . Binds to single-stranded DNA in an ATP-dependent manner to form nucleoprotein filaments which are essential for the homology search and strand exchange. Catalyzes the recognition of homology and strand exchange between homologous DNA partners to form a joint molecule between a processed DNA break and the repair template. Recruited to resolve stalled replication forks during replication stress. Part of a PALB2-scaffolded HR complex containing BRCA2 and RAD51C and which is thought to play a role in DNA repair by HR. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51C and XRCC3. Also involved in interstrand cross-link repair.
TTD ID
T63083
Uniprot ID
Q06609
DrugMap ID
TTC0G1L
Ensemble ID
ENST00000267868.8
HGNC ID
HGNC:9817
ChEMBL ID
CHEMBL2034807

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
HT SNV: p.E50D .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 6 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C31(1.08)  LDD3339  [1]
Acrolein
 Probe Info  		N.A.  LDD0222  [2]
ATP probe
 Probe Info  		N.A.  LDD0199  [3]
IA-alkyne
 Probe Info  		N.A.  LDD0165  [4]
Lodoacetamide azide
 Probe Info  		N.A.  LDD0037  [5]
AOyne
 Probe Info  		14.50  LDD0443  [6]
PAL-AfBPP Probe
Click To Hide/Show 2 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C135
 Probe Info  		10.78  LDD1817  [7]
VE-P
 Probe Info  		N.A.  LDD0396  [8]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0214  AC1 HEK-293T C319(0.86); C144(0.93)  LDD1507  [9]
 LDCM0215  AC10 HEK-293T C319(1.24); C144(1.19); C137(0.99)  LDD1508  [9]
 LDCM0237  AC12 HEK-293T C319(1.05); C144(1.05)  LDD1510  [9]
 LDCM0259  AC14 HEK-293T C319(0.87)  LDD1512  [9]
 LDCM0270  AC15 HEK-293T C144(1.00)  LDD1513  [9]
 LDCM0276  AC17 HEK-293T C319(1.18); C144(0.91)  LDD1515  [9]
 LDCM0277  AC18 HEK-293T C319(0.96); C144(1.32); C137(0.93)  LDD1516  [9]
 LDCM0279  AC2 HEK-293T C319(1.28); C144(1.06); C137(0.92)  LDD1518  [9]
 LDCM0280  AC20 HEK-293T C319(0.82); C144(0.93)  LDD1519  [9]
 LDCM0281  AC21 HEK-293T C144(1.04)  LDD1520  [9]
 LDCM0282  AC22 HEK-293T C319(0.88)  LDD1521  [9]
 LDCM0283  AC23 HEK-293T C144(0.97)  LDD1522  [9]
 LDCM0284  AC24 HEK-293T C319(1.00)  LDD1523  [9]
 LDCM0285  AC25 HEK-293T C319(1.03); C144(0.92)  LDD1524  [9]
 LDCM0286  AC26 HEK-293T C319(1.11); C144(0.99); C137(0.93)  LDD1525  [9]
 LDCM0288  AC28 HEK-293T C319(0.98); C144(0.94)  LDD1527  [9]
 LDCM0289  AC29 HEK-293T C144(0.92)  LDD1528  [9]
 LDCM0291  AC30 HEK-293T C319(0.98)  LDD1530  [9]
 LDCM0292  AC31 HEK-293T C144(0.97)  LDD1531  [9]
 LDCM0293  AC32 HEK-293T C319(0.91)  LDD1532  [9]
 LDCM0294  AC33 HEK-293T C319(1.01); C144(1.01)  LDD1533  [9]
 LDCM0295  AC34 HEK-293T C319(1.28); C144(1.31); C137(1.06)  LDD1534  [9]
 LDCM0297  AC36 HEK-293T C319(0.87); C144(0.93)  LDD1536  [9]
 LDCM0298  AC37 HEK-293T C144(0.99)  LDD1537  [9]
 LDCM0299  AC38 HEK-293T C319(0.82)  LDD1538  [9]
 LDCM0300  AC39 HEK-293T C144(1.07)  LDD1539  [9]
 LDCM0301  AC4 HEK-293T C319(0.99); C144(0.84)  LDD1540  [9]
 LDCM0302  AC40 HEK-293T C319(0.90)  LDD1541  [9]
 LDCM0303  AC41 HEK-293T C319(1.18); C144(0.95)  LDD1542  [9]
 LDCM0304  AC42 HEK-293T C319(1.19); C144(1.55); C137(1.00)  LDD1543  [9]
 LDCM0306  AC44 HEK-293T C319(1.03); C144(0.98)  LDD1545  [9]
 LDCM0307  AC45 HEK-293T C144(1.04)  LDD1546  [9]
 LDCM0308  AC46 HEK-293T C319(1.25)  LDD1547  [9]
 LDCM0309  AC47 HEK-293T C144(0.89)  LDD1548  [9]
 LDCM0310  AC48 HEK-293T C319(0.95)  LDD1549  [9]
 LDCM0311  AC49 HEK-293T C319(1.21); C144(0.95)  LDD1550  [9]
 LDCM0312  AC5 HEK-293T C144(0.89)  LDD1551  [9]
 LDCM0313  AC50 HEK-293T C319(1.11); C144(1.13); C137(0.96)  LDD1552  [9]
 LDCM0315  AC52 HEK-293T C319(0.93); C144(1.09)  LDD1554  [9]
 LDCM0316  AC53 HEK-293T C144(1.03)  LDD1555  [9]
 LDCM0317  AC54 HEK-293T C319(0.72)  LDD1556  [9]
 LDCM0318  AC55 HEK-293T C144(1.01)  LDD1557  [9]
 LDCM0319  AC56 HEK-293T C319(0.90)  LDD1558  [9]
 LDCM0320  AC57 HEK-293T C319(1.17); C144(1.02)  LDD1559  [9]
 LDCM0321  AC58 HEK-293T C319(1.42); C144(1.34); C137(1.03)  LDD1560  [9]
 LDCM0323  AC6 HEK-293T C319(0.88)  LDD1562  [9]
 LDCM0324  AC60 HEK-293T C319(0.90); C144(0.95)  LDD1563  [9]
 LDCM0325  AC61 HEK-293T C144(1.01)  LDD1564  [9]
 LDCM0326  AC62 HEK-293T C319(0.75)  LDD1565  [9]
 LDCM0327  AC63 HEK-293T C144(0.92)  LDD1566  [9]
 LDCM0328  AC64 HEK-293T C319(1.01)  LDD1567  [9]
 LDCM0334  AC7 HEK-293T C144(1.05)  LDD1568  [9]
 LDCM0345  AC8 HEK-293T C319(0.95)  LDD1569  [9]
 LDCM0248  AKOS034007472 HEK-293T C144(0.99)  LDD1511  [9]
 LDCM0356  AKOS034007680 HEK-293T C319(1.24); C144(0.95)  LDD1570  [9]
 LDCM0275  AKOS034007705 HEK-293T C319(1.01)  LDD1514  [9]
 LDCM0108  Chloroacetamide HeLa N.A.  LDD0222  [2]
 LDCM0367  CL1 HEK-293T C144(1.05)  LDD1571  [9]
 LDCM0368  CL10 HEK-293T C319(1.00)  LDD1572  [9]
 LDCM0370  CL101 HEK-293T C144(1.05)  LDD1574  [9]
 LDCM0371  CL102 HEK-293T C319(1.05); C144(0.87)  LDD1575  [9]
 LDCM0372  CL103 HEK-293T C144(1.01)  LDD1576  [9]
 LDCM0374  CL105 HEK-293T C144(0.98)  LDD1578  [9]
 LDCM0375  CL106 HEK-293T C319(0.87); C144(0.96)  LDD1579  [9]
 LDCM0376  CL107 HEK-293T C144(1.02)  LDD1580  [9]
 LDCM0378  CL109 HEK-293T C144(0.93)  LDD1582  [9]
 LDCM0379  CL11 HEK-293T C144(1.78)  LDD1583  [9]
 LDCM0380  CL110 HEK-293T C319(0.70); C144(0.96)  LDD1584  [9]
 LDCM0381  CL111 HEK-293T C144(0.92)  LDD1585  [9]
 LDCM0383  CL113 HEK-293T C144(1.02)  LDD1587  [9]
 LDCM0384  CL114 HEK-293T C319(0.83); C144(0.97)  LDD1588  [9]
 LDCM0385  CL115 HEK-293T C144(0.94)  LDD1589  [9]
 LDCM0387  CL117 HEK-293T C144(0.98)  LDD1591  [9]
 LDCM0388  CL118 HEK-293T C319(0.77); C144(0.93)  LDD1592  [9]
 LDCM0389  CL119 HEK-293T C144(1.03)  LDD1593  [9]
 LDCM0390  CL12 HEK-293T C319(1.00)  LDD1594  [9]
 LDCM0392  CL121 HEK-293T C144(1.04)  LDD1596  [9]
 LDCM0393  CL122 HEK-293T C319(0.95); C144(1.02)  LDD1597  [9]
 LDCM0394  CL123 HEK-293T C144(0.89)  LDD1598  [9]
 LDCM0396  CL125 HEK-293T C144(1.39)  LDD1600  [9]
 LDCM0397  CL126 HEK-293T C319(1.35); C144(0.98)  LDD1601  [9]
 LDCM0398  CL127 HEK-293T C144(1.02)  LDD1602  [9]
 LDCM0400  CL13 HEK-293T C144(1.09)  LDD1604  [9]
 LDCM0401  CL14 HEK-293T C319(0.98); C144(0.94)  LDD1605  [9]
 LDCM0402  CL15 HEK-293T C144(1.06)  LDD1606  [9]
 LDCM0404  CL17 HEK-293T C319(1.07); C144(1.17)  LDD1608  [9]
 LDCM0405  CL18 HEK-293T C319(1.02); C144(1.46); C137(1.27)  LDD1609  [9]
 LDCM0407  CL2 HEK-293T C319(0.89); C144(1.24)  LDD1611  [9]
 LDCM0408  CL20 HEK-293T C319(1.21); C144(1.83)  LDD1612  [9]
 LDCM0409  CL21 HEK-293T C144(2.01)  LDD1613  [9]
 LDCM0410  CL22 HEK-293T C319(0.69)  LDD1614  [9]
 LDCM0411  CL23 HEK-293T C144(1.96)  LDD1615  [9]
 LDCM0412  CL24 HEK-293T C319(0.96)  LDD1616  [9]
 LDCM0413  CL25 HEK-293T C144(1.09)  LDD1617  [9]
 LDCM0414  CL26 HEK-293T C319(0.78); C144(1.08)  LDD1618  [9]
 LDCM0415  CL27 HEK-293T C144(1.07)  LDD1619  [9]
 LDCM0417  CL29 HEK-293T C319(1.20); C144(1.24)  LDD1621  [9]
 LDCM0418  CL3 HEK-293T C144(1.26)  LDD1622  [9]
 LDCM0419  CL30 HEK-293T C319(1.12); C144(1.06); C137(1.47)  LDD1623  [9]
 LDCM0421  CL32 HEK-293T C319(0.91); C144(1.76)  LDD1625  [9]
 LDCM0422  CL33 HEK-293T C144(1.73)  LDD1626  [9]
 LDCM0423  CL34 HEK-293T C319(1.45)  LDD1627  [9]
 LDCM0424  CL35 HEK-293T C144(2.01)  LDD1628  [9]
 LDCM0425  CL36 HEK-293T C319(0.96)  LDD1629  [9]
 LDCM0426  CL37 HEK-293T C144(1.01)  LDD1630  [9]
 LDCM0428  CL39 HEK-293T C144(0.93)  LDD1632  [9]
 LDCM0431  CL41 HEK-293T C319(1.36); C144(1.13)  LDD1635  [9]
 LDCM0432  CL42 HEK-293T C319(1.18); C144(0.88); C137(1.38)  LDD1636  [9]
 LDCM0434  CL44 HEK-293T C319(1.22); C144(1.73)  LDD1638  [9]
 LDCM0435  CL45 HEK-293T C144(1.86)  LDD1639  [9]
 LDCM0436  CL46 HEK-293T C319(0.55)  LDD1640  [9]
 LDCM0437  CL47 HEK-293T C144(1.80)  LDD1641  [9]
 LDCM0438  CL48 HEK-293T C319(1.02)  LDD1642  [9]
 LDCM0439  CL49 HEK-293T C144(1.07)  LDD1643  [9]
 LDCM0440  CL5 HEK-293T C319(1.18); C144(1.42)  LDD1644  [9]
 LDCM0441  CL50 HEK-293T C319(0.93); C144(0.97)  LDD1645  [9]
 LDCM0444  CL53 HEK-293T C319(1.25); C144(0.99)  LDD1647  [9]
 LDCM0445  CL54 HEK-293T C319(1.21); C144(1.01); C137(1.15)  LDD1648  [9]
 LDCM0447  CL56 HEK-293T C319(1.46); C144(1.62)  LDD1650  [9]
 LDCM0448  CL57 HEK-293T C144(1.95)  LDD1651  [9]
 LDCM0449  CL58 HEK-293T C319(0.76)  LDD1652  [9]
 LDCM0450  CL59 HEK-293T C144(1.95)  LDD1653  [9]
 LDCM0451  CL6 HEK-293T C319(0.84); C144(0.94); C137(1.23)  LDD1654  [9]
 LDCM0452  CL60 HEK-293T C319(0.99)  LDD1655  [9]
 LDCM0453  CL61 HEK-293T C144(1.02)  LDD1656  [9]
 LDCM0454  CL62 HEK-293T C319(1.11); C144(1.06)  LDD1657  [9]
 LDCM0455  CL63 HEK-293T C144(0.97)  LDD1658  [9]
 LDCM0457  CL65 HEK-293T C319(1.42); C144(1.17)  LDD1660  [9]
 LDCM0458  CL66 HEK-293T C319(1.10); C144(1.03); C137(1.23)  LDD1661  [9]
 LDCM0460  CL68 HEK-293T C319(0.94); C144(1.48)  LDD1663  [9]
 LDCM0461  CL69 HEK-293T C144(1.75)  LDD1664  [9]
 LDCM0463  CL70 HEK-293T C319(0.85)  LDD1666  [9]
 LDCM0464  CL71 HEK-293T C144(1.59)  LDD1667  [9]
 LDCM0465  CL72 HEK-293T C319(1.21)  LDD1668  [9]
 LDCM0466  CL73 HEK-293T C144(0.96)  LDD1669  [9]
 LDCM0467  CL74 HEK-293T C319(0.99); C144(0.89)  LDD1670  [9]
 LDCM0470  CL77 HEK-293T C319(0.89); C144(0.99)  LDD1673  [9]
 LDCM0471  CL78 HEK-293T C319(0.98); C144(1.58); C137(1.29)  LDD1674  [9]
 LDCM0473  CL8 HEK-293T C319(0.84); C144(1.24)  LDD1676  [9]
 LDCM0474  CL80 HEK-293T C319(0.96); C144(1.40)  LDD1677  [9]
 LDCM0475  CL81 HEK-293T C144(1.75)  LDD1678  [9]
 LDCM0476  CL82 HEK-293T C319(1.00)  LDD1679  [9]
 LDCM0477  CL83 HEK-293T C144(1.53)  LDD1680  [9]
 LDCM0478  CL84 HEK-293T C319(0.88)  LDD1681  [9]
 LDCM0479  CL85 HEK-293T C144(1.14)  LDD1682  [9]
 LDCM0480  CL86 HEK-293T C319(1.01); C144(1.01)  LDD1683  [9]
 LDCM0481  CL87 HEK-293T C144(1.03)  LDD1684  [9]
 LDCM0483  CL89 HEK-293T C319(1.53); C144(1.13)  LDD1686  [9]
 LDCM0484  CL9 HEK-293T C144(2.03)  LDD1687  [9]
 LDCM0485  CL90 HEK-293T C319(1.11); C144(1.08); C137(1.11)  LDD1688  [9]
 LDCM0487  CL92 HEK-293T C319(0.82); C144(1.36)  LDD1690  [9]
 LDCM0488  CL93 HEK-293T C144(1.59)  LDD1691  [9]
 LDCM0489  CL94 HEK-293T C319(0.77)  LDD1692  [9]
 LDCM0490  CL95 HEK-293T C144(1.22)  LDD1693  [9]
 LDCM0491  CL96 HEK-293T C319(1.02)  LDD1694  [9]
 LDCM0492  CL97 HEK-293T C144(0.99)  LDD1695  [9]
 LDCM0493  CL98 HEK-293T C319(0.83); C144(0.93)  LDD1696  [9]
 LDCM0494  CL99 HEK-293T C144(0.86)  LDD1697  [9]
 LDCM0495  E2913 HEK-293T C144(0.96)  LDD1698  [9]
 LDCM0468  Fragment33 HEK-293T C144(0.91)  LDD1671  [9]
 LDCM0427  Fragment51 HEK-293T C319(0.93); C144(0.92)  LDD1631  [9]
 LDCM0022  KB02 697 C31(1.05)  LDD2245  [1]
 LDCM0023  KB03 697 C31(1.22)  LDD2662  [1]
 LDCM0024  KB05 NALM-6 C31(1.08)  LDD3339  [1]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 3 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Arylamine N-acetyltransferase 2 (NAT2) Arylamine N-acetyltransferase family P11245
Histone-lysine N-methyltransferase NSD3 (NSD3) Histone-lysine methyltransferase family Q9BZ95
Serine/threonine-protein kinase Chk1 (CHEK1) CAMK Ser/Thr protein kinase family O14757
Transporter and channel
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Cellular tumor antigen p53 (TP53) P53 family P04637
Cytokine and receptor
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Interleukin-24 (IL24) IL-10 family Q13007
Other
Click To Hide/Show 11 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Protein LRATD2 (LRATD2) LRATD family Q96KN1
DNA repair protein RAD52 homolog (RAD52) RAD52 family P43351
DNA repair protein RAD51 homolog 1 (RAD51) RecA family Q06609
DNA repair protein RAD51 homolog 3 (RAD51C) RecA family O43502
DNA repair protein XRCC3 (XRCC3) RecA family O43542
ATPase SWSAP1 (SWSAP1) . Q6NVH7
Breast cancer type 2 susceptibility protein (BRCA2) . P51587
DNA repair-scaffolding protein (SPIDR) . Q14159
Mediator of DNA damage checkpoint protein 1 (MDC1) . Q14676
Partner and localizer of BRCA2 (PALB2) . Q86YC2
Vigilin (HDLBP) . Q00341

The Drug(s) Related To This Target

Phase 2
Click To Hide/Show 1 Drug(s) Interacting with This Target
Drug Name Drug Type External ID
Cyt-0851 Small molecular drug DR5VQ1
Investigative
Click To Hide/Show 3 Drug(s) Interacting with This Target
Drug Name Drug Type External ID
Amp-pnp Small molecular drug D00ICA
Amuvatinib Small molecular drug DB12742
Phosphoaminophosphonic Acid-adenylate Ester . DB04395

References

1 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
2 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
3 Targeted Proteomic Approaches for Proteome-Wide Characterizations of the AMP-Binding Capacities of Kinases. J Proteome Res. 2022 Aug 5;21(8):2063-2070. doi: 10.1021/acs.jproteome.2c00225. Epub 2022 Jul 12.
4 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
5 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
6 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.
7 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587
8 Pharmacological Targeting of Vacuolar H(+)-ATPase via Subunit V1G Combats Multidrug-Resistant Cancer. Cell Chem Biol. 2020 Nov 19;27(11):1359-1370.e8. doi: 10.1016/j.chembiol.2020.06.011. Epub 2020 Jul 9.
9 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402