Details of the Target

General Information of Target

Target ID LDTP04540
Target Name Bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 1 (NDST1)
Gene Name NDST1
Gene ID 3340
Synonyms
HSST; HSST1; Bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 1; Glucosaminyl N-deacetylase/N-sulfotransferase 1; NDST-1; N-heparan sulfate sulfotransferase 1; N-HSST 1; [Heparan sulfate]-glucosamine N-sulfotransferase 1; HSNST 1) [Includes: Heparan sulfate N-deacetylase 1; EC 3.5.1.-; Heparan sulfate N-sulfotransferase 1; EC 2.8.2.8)]
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MPALACLRRLCRHVSPQAVLFLLFIFCLFSVFISAYYLYGWKRGLEPSADAPEPDCGDPP
PVAPSRLLPLKPVQAATPSRTDPLVLVFVESLYSQLGQEVVAILESSRFKYRTEIAPGKG
DMPTLTDKGRGRFALIIYENILKYVNLDAWNRELLDKYCVAYGVGIIGFFKANENSLLSA
QLKGFPLFLHSNLGLKDCSINPKSPLLYVTRPSEVEKGVLPGEDWTVFQSNHSTYEPVLL
AKTRSSESIPHLGADAGLHAALHATVVQDLGLHDGIQRVLFGNNLNFWLHKLVFVDAVAF
LTGKRLSLPLDRYILVDIDDIFVGKEGTRMKVEDVKALFDTQNELRAHIPNFTFNLGYSG
KFFHTGTNAEDAGDDLLLSYVKEFWWFPHMWSHMQPHLFHNQSVLAEQMALNKKFAVEHG
IPTDMGYAVAPHHSGVYPVHVQLYEAWKQVWSIRVTSTEEYPHLKPARYRRGFIHNGIMV
LPRQTCGLFTHTIFYNEYPGGSSELDKIINGGELFLTVLLNPISIFMTHLSNYGNDRLGL
YTFKHLVRFLHSWTNLRLQTLPPVQLAQKYFQIFSEEKDPLWQDPCEDKRHKDIWSKEKT
CDRFPKLLIIGPQKTGTTALYLFLGMHPDLSSNYPSSETFEEIQFFNGHNYHKGIDWYME
FFPIPSNTTSDFYFEKSANYFDSEVAPRRAAALLPKAKVLTILINPADRAYSWYQHQRAH
DDPVALKYTFHEVITAGSDASSKLRALQNRCLVPGWYATHIERWLSAYHANQILVLDGKL
LRTEPAKVMDMVQKFLGVTNTIDYHKTLAFDPKKGFWCQLLEGGKTKCLGKSKGRKYPEM
DLDSRAFLKDYYRDHNIELSKLLYKMGQTLPTWLREDLQNTR
Target Bioclass
Enzyme
Family
Sulfotransferase 1 family, NDST subfamily
Subcellular location
Golgi apparatus, trans-Golgi network membrane; Golgi apparatus, cis-Golgi network membrane
Function
[Isoform 1]: Essential bifunctional enzyme that catalyzes both the N-deacetylation and the N-sulfation of glucosamine (GlcNAc) of the glycosaminoglycan in heparan sulfate. Modifies the GlcNAc-GlcA disaccharide repeating sugar backbone to make N-sulfated heparosan, a prerequisite substrate for later modifications in heparin biosynthesis. Plays a role in determining the extent and pattern of sulfation of heparan sulfate. Participates in biosynthesis of heparan sulfate that can ultimately serve as L-selectin ligands, thereby playing a role in inflammatory response. Required for the exosomal release of SDCBP, CD63 and syndecan.; [Isoform 3]: Lacks both N-deacetylase and N-sulfotransferase activities. Acts as a dominant negative on isoform 1, likely by changing the composition of enzyme complexes responsible for elongation and modification of heparan sulfates.
Uniprot ID
P52848
Ensemble ID
ENST00000261797.7
HGNC ID
HGNC:7680

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
A204 Deletion: p.G755PfsTer30 .
COLO320 Deletion: p.P83RfsTer96 .
MCC13 SNV: p.E587K .
NCIH1155 SNV: p.H463P .
NCIH2172 SNV: p.V455L .
NCIH358 SNV: p.S307P .
SUPT1 SNV: p.A149T .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 3 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C818(0.91)  LDD1095  [1]
IA-alkyne
 Probe Info  		N.A.  LDD0165  [2]
NAIA_5
 Probe Info  		N.A.  LDD2223  [3]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0215  AC10 HEK-293T C601(1.02)  LDD1508  [4]
 LDCM0216  AC100 PaTu 8988t C818(0.91)  LDD1095  [1]
 LDCM0217  AC101 PaTu 8988t C818(1.20)  LDD1096  [1]
 LDCM0218  AC102 PaTu 8988t C818(0.92)  LDD1097  [1]
 LDCM0219  AC103 PaTu 8988t C818(1.13)  LDD1098  [1]
 LDCM0220  AC104 PaTu 8988t C818(1.07)  LDD1099  [1]
 LDCM0221  AC105 PaTu 8988t C818(1.07)  LDD1100  [1]
 LDCM0222  AC106 PaTu 8988t C818(0.92)  LDD1101  [1]
 LDCM0223  AC107 PaTu 8988t C818(0.82)  LDD1102  [1]
 LDCM0224  AC108 PaTu 8988t C818(0.88)  LDD1103  [1]
 LDCM0225  AC109 PaTu 8988t C818(0.94)  LDD1104  [1]
 LDCM0226  AC11 HEK-293T C601(1.14)  LDD1509  [4]
 LDCM0227  AC110 PaTu 8988t C818(1.09)  LDD1106  [1]
 LDCM0228  AC111 PaTu 8988t C818(0.85)  LDD1107  [1]
 LDCM0229  AC112 PaTu 8988t C818(0.98)  LDD1108  [1]
 LDCM0230  AC113 PaTu 8988t C818(0.86)  LDD1109  [1]
 LDCM0231  AC114 PaTu 8988t C818(0.79)  LDD1110  [1]
 LDCM0232  AC115 PaTu 8988t C818(0.77)  LDD1111  [1]
 LDCM0233  AC116 PaTu 8988t C818(0.88)  LDD1112  [1]
 LDCM0234  AC117 PaTu 8988t C818(0.78)  LDD1113  [1]
 LDCM0235  AC118 PaTu 8988t C818(0.88)  LDD1114  [1]
 LDCM0236  AC119 PaTu 8988t C818(0.95)  LDD1115  [1]
 LDCM0238  AC120 PaTu 8988t C818(0.82)  LDD1117  [1]
 LDCM0239  AC121 PaTu 8988t C818(0.79)  LDD1118  [1]
 LDCM0240  AC122 PaTu 8988t C818(0.80)  LDD1119  [1]
 LDCM0241  AC123 PaTu 8988t C818(0.73)  LDD1120  [1]
 LDCM0242  AC124 PaTu 8988t C818(0.74)  LDD1121  [1]
 LDCM0243  AC125 PaTu 8988t C818(0.85)  LDD1122  [1]
 LDCM0244  AC126 PaTu 8988t C818(0.83)  LDD1123  [1]
 LDCM0245  AC127 PaTu 8988t C818(0.79)  LDD1124  [1]
 LDCM0259  AC14 HEK-293T C601(1.15)  LDD1512  [4]
 LDCM0263  AC143 PaTu 8988t C818(0.91)  LDD1142  [1]
 LDCM0264  AC144 PaTu 8988t C818(1.01)  LDD1143  [1]
 LDCM0265  AC145 PaTu 8988t C818(1.00)  LDD1144  [1]
 LDCM0266  AC146 PaTu 8988t C818(0.91)  LDD1145  [1]
 LDCM0267  AC147 PaTu 8988t C818(1.03)  LDD1146  [1]
 LDCM0268  AC148 PaTu 8988t C818(1.00)  LDD1147  [1]
 LDCM0269  AC149 PaTu 8988t C818(0.88)  LDD1148  [1]
 LDCM0270  AC15 HEK-293T C601(1.23)  LDD1513  [4]
 LDCM0271  AC150 PaTu 8988t C818(1.09)  LDD1150  [1]
 LDCM0272  AC151 PaTu 8988t C818(0.99)  LDD1151  [1]
 LDCM0273  AC152 PaTu 8988t C818(0.93)  LDD1152  [1]
 LDCM0274  AC153 PaTu 8988t C818(0.88)  LDD1153  [1]
 LDCM0621  AC154 PaTu 8988t C818(0.97)  LDD2166  [1]
 LDCM0622  AC155 PaTu 8988t C818(0.99)  LDD2167  [1]
 LDCM0623  AC156 PaTu 8988t C818(0.92)  LDD2168  [1]
 LDCM0624  AC157 PaTu 8988t C818(0.89)  LDD2169  [1]
 LDCM0277  AC18 HEK-293T C601(0.94)  LDD1516  [4]
 LDCM0278  AC19 HEK-293T C601(0.98)  LDD1517  [4]
 LDCM0279  AC2 HEK-293T C601(1.02)  LDD1518  [4]
 LDCM0282  AC22 HEK-293T C601(1.06)  LDD1521  [4]
 LDCM0283  AC23 HEK-293T C601(1.14)  LDD1522  [4]
 LDCM0284  AC24 HEK-293T C601(1.16); C818(1.05)  LDD1523  [4]
 LDCM0286  AC26 HEK-293T C601(1.01)  LDD1525  [4]
 LDCM0287  AC27 HEK-293T C601(1.00)  LDD1526  [4]
 LDCM0290  AC3 HEK-293T C601(0.93)  LDD1529  [4]
 LDCM0291  AC30 HEK-293T C601(1.01)  LDD1530  [4]
 LDCM0292  AC31 HEK-293T C601(1.08)  LDD1531  [4]
 LDCM0293  AC32 HEK-293T C601(1.04); C818(1.16)  LDD1532  [4]
 LDCM0295  AC34 HEK-293T C601(1.02)  LDD1534  [4]
 LDCM0296  AC35 HEK-293T C601(1.01)  LDD1535  [4]
 LDCM0299  AC38 HEK-293T C601(1.18)  LDD1538  [4]
 LDCM0300  AC39 HEK-293T C601(1.18)  LDD1539  [4]
 LDCM0302  AC40 HEK-293T C601(1.16); C818(1.02)  LDD1541  [4]
 LDCM0304  AC42 HEK-293T C601(0.94)  LDD1543  [4]
 LDCM0305  AC43 HEK-293T C601(1.20)  LDD1544  [4]
 LDCM0308  AC46 PaTu 8988t C818(1.03)  LDD1187  [1]
 LDCM0309  AC47 PaTu 8988t C818(1.00)  LDD1188  [1]
 LDCM0310  AC48 PaTu 8988t C818(1.03)  LDD1189  [1]
 LDCM0311  AC49 PaTu 8988t C818(1.04)  LDD1190  [1]
 LDCM0313  AC50 PaTu 8988t C818(1.04)  LDD1192  [1]
 LDCM0314  AC51 PaTu 8988t C818(0.67)  LDD1193  [1]
 LDCM0315  AC52 PaTu 8988t C818(1.05)  LDD1194  [1]
 LDCM0316  AC53 PaTu 8988t C818(1.01)  LDD1195  [1]
 LDCM0317  AC54 PaTu 8988t C818(0.86)  LDD1196  [1]
 LDCM0318  AC55 PaTu 8988t C818(0.99)  LDD1197  [1]
 LDCM0319  AC56 PaTu 8988t C818(1.14)  LDD1198  [1]
 LDCM0321  AC58 HEK-293T C601(0.98)  LDD1560  [4]
 LDCM0322  AC59 HEK-293T C601(1.03)  LDD1561  [4]
 LDCM0323  AC6 HEK-293T C601(0.93)  LDD1562  [4]
 LDCM0326  AC62 HEK-293T C601(0.96)  LDD1565  [4]
 LDCM0327  AC63 HEK-293T C601(1.03)  LDD1566  [4]
 LDCM0328  AC64 HEK-293T C601(1.05); C818(1.03)  LDD1567  [4]
 LDCM0334  AC7 HEK-293T C601(1.14)  LDD1568  [4]
 LDCM0345  AC8 HEK-293T C601(1.01); C818(1.06)  LDD1569  [4]
 LDCM0365  AC98 PaTu 8988t C818(0.88)  LDD1244  [1]
 LDCM0366  AC99 PaTu 8988t C818(0.93)  LDD1245  [1]
 LDCM0275  AKOS034007705 HEK-293T C601(1.27); C818(1.04)  LDD1514  [4]
 LDCM0368  CL10 HEK-293T C601(1.96)  LDD1572  [4]
 LDCM0369  CL100 HEK-293T C601(0.91)  LDD1573  [4]
 LDCM0371  CL102 HEK-293T C601(0.97)  LDD1575  [4]
 LDCM0372  CL103 HEK-293T C601(1.16)  LDD1576  [4]
 LDCM0373  CL104 HEK-293T C601(1.07)  LDD1577  [4]
 LDCM0375  CL106 HEK-293T C601(1.04)  LDD1579  [4]
 LDCM0376  CL107 HEK-293T C601(1.11)  LDD1580  [4]
 LDCM0377  CL108 HEK-293T C601(1.05)  LDD1581  [4]
 LDCM0379  CL11 HEK-293T C601(2.44)  LDD1583  [4]
 LDCM0380  CL110 HEK-293T C601(1.07)  LDD1584  [4]
 LDCM0381  CL111 HEK-293T C601(1.04)  LDD1585  [4]
 LDCM0382  CL112 HEK-293T C601(0.94)  LDD1586  [4]
 LDCM0384  CL114 HEK-293T C601(0.91)  LDD1588  [4]
 LDCM0385  CL115 HEK-293T C601(1.10)  LDD1589  [4]
 LDCM0386  CL116 HEK-293T C601(1.08)  LDD1590  [4]
 LDCM0388  CL118 HEK-293T C601(1.12)  LDD1592  [4]
 LDCM0389  CL119 HEK-293T C601(1.19)  LDD1593  [4]
 LDCM0390  CL12 HEK-293T C601(2.38); C818(1.07)  LDD1594  [4]
 LDCM0391  CL120 HEK-293T C601(1.07)  LDD1595  [4]
 LDCM0392  CL121 PaTu 8988t C818(1.19)  LDD1271  [1]
 LDCM0393  CL122 PaTu 8988t C818(1.31)  LDD1272  [1]
 LDCM0394  CL123 PaTu 8988t C818(1.06)  LDD1273  [1]
 LDCM0395  CL124 PaTu 8988t C818(1.17)  LDD1274  [1]
 LDCM0397  CL126 HEK-293T C601(0.92)  LDD1601  [4]
 LDCM0398  CL127 HEK-293T C601(0.98)  LDD1602  [4]
 LDCM0399  CL128 HEK-293T C601(0.93)  LDD1603  [4]
 LDCM0401  CL14 HEK-293T C601(1.13)  LDD1605  [4]
 LDCM0402  CL15 HEK-293T C601(1.20)  LDD1606  [4]
 LDCM0403  CL16 HEK-293T C601(1.33)  LDD1607  [4]
 LDCM0405  CL18 HEK-293T C601(1.50)  LDD1609  [4]
 LDCM0406  CL19 HEK-293T C601(1.85)  LDD1610  [4]
 LDCM0407  CL2 HEK-293T C601(1.16)  LDD1611  [4]
 LDCM0410  CL22 HEK-293T C601(2.66)  LDD1614  [4]
 LDCM0411  CL23 HEK-293T C601(2.85)  LDD1615  [4]
 LDCM0412  CL24 HEK-293T C601(2.73); C818(1.03)  LDD1616  [4]
 LDCM0414  CL26 HEK-293T C601(1.17)  LDD1618  [4]
 LDCM0415  CL27 HEK-293T C601(1.30)  LDD1619  [4]
 LDCM0416  CL28 HEK-293T C601(1.28)  LDD1620  [4]
 LDCM0418  CL3 HEK-293T C601(1.28)  LDD1622  [4]
 LDCM0419  CL30 HEK-293T C601(1.53)  LDD1623  [4]
 LDCM0420  CL31 HEK-293T C601(1.79)  LDD1624  [4]
 LDCM0423  CL34 HEK-293T C601(2.45)  LDD1627  [4]
 LDCM0424  CL35 HEK-293T C601(3.17)  LDD1628  [4]
 LDCM0425  CL36 HEK-293T C601(2.64); C818(0.71)  LDD1629  [4]
 LDCM0428  CL39 HEK-293T C601(1.12)  LDD1632  [4]
 LDCM0429  CL4 HEK-293T C601(1.09)  LDD1633  [4]
 LDCM0430  CL40 HEK-293T C601(1.19)  LDD1634  [4]
 LDCM0432  CL42 HEK-293T C601(1.28)  LDD1636  [4]
 LDCM0433  CL43 HEK-293T C601(1.86)  LDD1637  [4]
 LDCM0436  CL46 HEK-293T C601(2.25)  LDD1640  [4]
 LDCM0437  CL47 HEK-293T C601(2.73)  LDD1641  [4]
 LDCM0438  CL48 HEK-293T C601(2.47); C818(1.01)  LDD1642  [4]
 LDCM0441  CL50 HEK-293T C601(1.15)  LDD1645  [4]
 LDCM0443  CL52 HEK-293T C601(1.29)  LDD1646  [4]
 LDCM0445  CL54 HEK-293T C601(1.37)  LDD1648  [4]
 LDCM0446  CL55 HEK-293T C601(1.97)  LDD1649  [4]
 LDCM0449  CL58 HEK-293T C601(2.70)  LDD1652  [4]
 LDCM0450  CL59 HEK-293T C601(2.76)  LDD1653  [4]
 LDCM0451  CL6 HEK-293T C601(1.21)  LDD1654  [4]
 LDCM0452  CL60 HEK-293T C601(2.70); C818(0.97)  LDD1655  [4]
 LDCM0454  CL62 HEK-293T C601(1.09)  LDD1657  [4]
 LDCM0455  CL63 HEK-293T C601(1.21)  LDD1658  [4]
 LDCM0456  CL64 HEK-293T C601(1.12)  LDD1659  [4]
 LDCM0458  CL66 HEK-293T C601(1.29)  LDD1661  [4]
 LDCM0459  CL67 HEK-293T C601(1.55)  LDD1662  [4]
 LDCM0462  CL7 HEK-293T C601(1.44)  LDD1665  [4]
 LDCM0463  CL70 HEK-293T C601(2.17)  LDD1666  [4]
 LDCM0464  CL71 HEK-293T C601(2.44)  LDD1667  [4]
 LDCM0465  CL72 HEK-293T C601(2.57); C818(0.84)  LDD1668  [4]
 LDCM0467  CL74 HEK-293T C601(1.17)  LDD1670  [4]
 LDCM0469  CL76 HEK-293T C601(1.16)  LDD1672  [4]
 LDCM0471  CL78 HEK-293T C601(1.35)  LDD1674  [4]
 LDCM0472  CL79 HEK-293T C601(1.60)  LDD1675  [4]
 LDCM0476  CL82 HEK-293T C601(2.03)  LDD1679  [4]
 LDCM0477  CL83 HEK-293T C601(1.91)  LDD1680  [4]
 LDCM0478  CL84 HEK-293T C601(1.71); C818(1.16)  LDD1681  [4]
 LDCM0480  CL86 HEK-293T C601(0.96)  LDD1683  [4]
 LDCM0481  CL87 HEK-293T C601(1.00)  LDD1684  [4]
 LDCM0482  CL88 HEK-293T C601(1.12)  LDD1685  [4]
 LDCM0485  CL90 HEK-293T C601(0.98)  LDD1688  [4]
 LDCM0486  CL91 HEK-293T C601(1.38)  LDD1689  [4]
 LDCM0489  CL94 HEK-293T C601(1.69)  LDD1692  [4]
 LDCM0490  CL95 HEK-293T C601(1.38)  LDD1693  [4]
 LDCM0491  CL96 HEK-293T C601(1.43); C818(1.11)  LDD1694  [4]
 LDCM0493  CL98 HEK-293T C601(0.95)  LDD1696  [4]
 LDCM0494  CL99 HEK-293T C601(1.06)  LDD1697  [4]
 LDCM0495  E2913 HEK-293T C601(1.21)  LDD1698  [4]
 LDCM0468  Fragment33 HEK-293T C601(1.16)  LDD1671  [4]
 LDCM0427  Fragment51 HEK-293T C601(1.09)  LDD1631  [4]
 LDCM0022  KB02 BRX29 C198(2.37)  LDD2270  [5]
 LDCM0023  KB03 BRX29 C198(2.98)  LDD2687  [5]
 LDCM0024  KB05 BRX29 C198(2.66)  LDD3104  [5]

The Interaction Atlas With This Target

The Drug(s) Related To This Target

Investigative
Click To Hide/Show 1 Drug(s) Interacting with This Target
Drug Name Drug Type External ID
Adenosine 3'5'-diphosphate Small molecular drug DB01812

References

1 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
2 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
3 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
4 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
5 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840