Details of the Target

General Information of Target

Target ID LDTP01054
Target Name Histone H2B type 1-K (H2BC12)
Gene Name H2BC12
Gene ID 85236
Synonyms
H2BFT; HIRIP1; HIST1H2BK; Histone H2B type 1-K; H2B K; HIRA-interacting protein 1
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MPEPAKSAPAPKKGSKKAVTKAQKKDGKKRKRSRKESYSVYVYKVLKQVHPDTGISSKAM
GIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLPGELAKHAVSEGTKAVT
KYTSAK
Target Bioclass
Other
Family
Histone H2B family
Subcellular location
Nucleus
Function
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.; Has broad antibacterial activity. May contribute to the formation of the functional antimicrobial barrier of the colonic epithelium, and to the bactericidal activity of amniotic fluid.
Uniprot ID
O60814
Ensemble ID
ENST00000356950.2
HGNC ID
HGNC:13954

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 15 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
TH211
 Probe Info  		Y84(16.42); Y38(11.06); Y43(10.74); Y41(6.07)  LDD0257  [1]
TH216
 Probe Info  		Y38(20.00); Y43(20.00); Y84(20.00); Y41(10.90)  LDD0259  [1]
OPA-S-S-alkyne
 Probe Info  		K86(8.96); K109(11.43)  LDD3494  [2]
Probe 1
 Probe Info  		Y41(140.90); Y84(61.09)  LDD3495  [3]
HHS-475
 Probe Info  		Y84(1.13)  LDD0264  [4]
HHS-465
 Probe Info  		Y84(2.95)  LDD2237  [5]
5E-2FA
 Probe Info  		N.A.  LDD2235  [6]
m-APA
 Probe Info  		N.A.  LDD2231  [6]
aONE
 Probe Info  		K109(0.00); K117(0.00)  LDD0002  [7]
OSF
 Probe Info  		Y84(0.00); H83(0.00); Y41(0.00); H110(0.00)  LDD0029  [8]
SF
 Probe Info  		K117(0.00); K12(0.00); Y38(0.00); K121(0.00)  LDD0028  [8]
1c-yne
 Probe Info  		K44(0.00); K58(0.00); K117(0.00)  LDD0228  [9]
Acrolein
 Probe Info  		N.A.  LDD0217  [10]
Crotonaldehyde
 Probe Info  		N.A.  LDD0219  [10]
Methacrolein
 Probe Info  		N.A.  LDD0218  [10]
PAL-AfBPP Probe
Click To Hide/Show 4 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
VE-P
 Probe Info  		N.A.  LDD0396  [11]
Photocelecoxib
 Probe Info  		H50(0.00); S65(0.00); V67(0.00); D69(0.00)  LDD0019  [12]
Photoindomethacin
 Probe Info  		N.A.  LDD0155  [12]
Photonaproxen
 Probe Info  		D69(0.00); F71(0.00); E72(0.00); R73(0.00)  LDD0156  [12]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0108  Chloroacetamide HeLa N.A.  LDD0222  [10]
 LDCM0116  HHS-0101 DM93 Y84(1.13)  LDD0264  [4]
 LDCM0117  HHS-0201 DM93 Y84(1.00)  LDD0265  [4]
 LDCM0118  HHS-0301 DM93 Y84(1.27)  LDD0266  [4]
 LDCM0119  HHS-0401 DM93 Y84(1.26)  LDD0267  [4]
 LDCM0120  HHS-0701 DM93 Y84(1.74)  LDD0268  [4]
 LDCM0107  IAA HeLa N.A.  LDD0221  [10]
 LDCM0109  NEM HeLa N.A.  LDD0223  [10]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Other
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily B member 1 (SMARCB1) SNF5 family Q12824

References

1 Chemoproteomic profiling of kinases in live cells using electrophilic sulfonyl triazole probes. Chem Sci. 2021 Jan 21;12(9):3295-3307. doi: 10.1039/d0sc06623k.
2 A chemical proteomics approach for global mapping of functional lysines on cell surface of living cell. Nat Commun. 2024 Apr 8;15(1):2997. doi: 10.1038/s41467-024-47033-w.
Mass spectrometry data entry: PXD042888
3 An Azo Coupling-Based Chemoproteomic Approach to Systematically Profile the Tyrosine Reactivity in the Human Proteome. Anal Chem. 2021 Jul 27;93(29):10334-10342. doi: 10.1021/acs.analchem.1c01935. Epub 2021 Jul 12.
4 Discovery of a Cell-Active SuTEx Ligand of Prostaglandin Reductase 2. Chembiochem. 2021 Jun 15;22(12):2134-2139. doi: 10.1002/cbic.202000879. Epub 2021 Apr 29.
5 Global targeting of functional tyrosines using sulfur-triazole exchange chemistry. Nat Chem Biol. 2020 Feb;16(2):150-159. doi: 10.1038/s41589-019-0404-5. Epub 2019 Nov 25.
6 Global profiling of functional histidines in live cells using small-molecule photosensitizer and chemical probe relay labelling. Nat Chem. 2024 Jun 4. doi: 10.1038/s41557-024-01545-6. Online ahead of print.
Mass spectrometry data entry: PXD042377
7 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
8 Solid Phase Synthesis of Fluorosulfate Containing Macrocycles for Chemoproteomic Workflows. bioRxiv [Preprint]. 2023 Feb 18:2023.02.17.529022. doi: 10.1101/2023.02.17.529022.
Mass spectrometry data entry: PXD039931
9 Tunable Amine-Reactive Electrophiles for Selective Profiling of Lysine. Angew Chem Int Ed Engl. 2022 Jan 26;61(5):e202112107. doi: 10.1002/anie.202112107. Epub 2021 Dec 16.
10 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
11 Pharmacological Targeting of Vacuolar H(+)-ATPase via Subunit V1G Combats Multidrug-Resistant Cancer. Cell Chem Biol. 2020 Nov 19;27(11):1359-1370.e8. doi: 10.1016/j.chembiol.2020.06.011. Epub 2020 Jul 9.
12 Small Molecule Interactome Mapping by Photoaffinity Labeling Reveals Binding Site Hotspots for the NSAIDs. J Am Chem Soc. 2018 Mar 28;140(12):4259-4268. doi: 10.1021/jacs.7b11639. Epub 2018 Mar 15.
Mass spectrometry data entry: PXD007094