Details of the Target

General Information of Target

Target ID LDTP00603
Target Name Protein phosphatase 1D (PPM1D)
Gene Name PPM1D
Gene ID 8493
Synonyms
WIP1; Protein phosphatase 1D; EC 3.1.3.16; Protein phosphatase 2C isoform delta; PP2C-delta; Protein phosphatase magnesium-dependent 1 delta; p53-induced protein phosphatase 1
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MAGLYSLGVSVFSDQGGRKYMEDVTQIVVEPEPTAEEKPSPRRSLSQPLPPRPSPAALPG
GEVSGKGPAVAAREARDPLPDAGASPAPSRCCRRRSSVAFFAVCDGHGGREAAQFAREHL
WGFIKKQKGFTSSEPAKVCAAIRKGFLACHLAMWKKLAEWPKTMTGLPSTSGTTASVVII
RGMKMYVAHVGDSGVVLGIQDDPKDDFVRAVEVTQDHKPELPKERERIEGLGGSVMNKSG
VNRVVWKRPRLTHNGPVRRSTVIDQIPFLAVARALGDLWSYDFFSGEFVVSPEPDTSVHT
LDPQKHKYIILGSDGLWNMIPPQDAISMCQDQEEKKYLMGEHGQSCAKMLVNRALGRWRQ
RMLRADNTSAIVICISPEVDNQGNFTNEDELYLNLTDSPSYNSQETCVMTPSPCSTPPVK
SLEEDPWPRVNSKDHIPALVRSNAFSENFLEVSAEIARENVQGVVIPSKDPEPLEENCAK
ALTLRIHDSLNNSLPIGLVPTNSTNTVMDQKNLKMSTPGQMKAQEIERTPPTNFKRTLEE
SNSGPLMKKHRRNGLSRSSGAQPASLPTTSQRKNSVKLTMRRRLRGQKKIGNPLLHQHRK
TVCVC
Target Type
Literature-reported
Target Bioclass
Enzyme
Family
PP2C family
Subcellular location
Nucleus
Function
Involved in the negative regulation of p53 expression. Required for the relief of p53-dependent checkpoint mediated cell cycle arrest. Binds to and dephosphorylates 'Ser-15' of TP53 and 'Ser-345' of CHEK1 which contributes to the functional inactivation of these proteins. Mediates MAPK14 dephosphorylation and inactivation. Is also an important regulator of global heterochromatin silencing and critical in maintaining genome integrity.
TTD ID
T50914
Uniprot ID
O15297
DrugMap ID
TTENJAB
Ensemble ID
ENST00000305921.8
HGNC ID
HGNC:9277
ChEMBL ID
CHEMBL1938224

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
C32 SNV: p.P267S .
CAL51 SNV: p.K38Q .
CCK81 Deletion: p.N512IfsTer2 .
HCT116 Deletion: p.L450Ter .
HT115 SNV: p.I320L .
MELJUSO SNV: p.E111G .
NCIH1048 SNV: p.H342Y .
NCIH3122 Insertion: p.N512KfsTer16 .
NCIH661 SNV: p.R557L .
SKNSH SNV: p.S6Ter .
SUPT1 Insertion: p.N512KfsTer16 .
TOV21G SNV: p.G16C; p.E22Ter .
U2OS SNV: p.R458Ter .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 4 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C104(0.91)  LDD0547  [1]
IA-alkyne
 Probe Info  		N.A.  LDD0165  [2]
IPM
 Probe Info  		N.A.  LDD2156  [3]
NAIA_5
 Probe Info  		C104(0.00); C346(0.00)  LDD2223  [4]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0230  AC113 HCT 116 C104(0.91)  LDD0547  [1]
 LDCM0231  AC114 HCT 116 C104(0.92)  LDD0548  [1]
 LDCM0232  AC115 HCT 116 C104(0.92)  LDD0549  [1]
 LDCM0233  AC116 HCT 116 C104(0.86)  LDD0550  [1]
 LDCM0234  AC117 HCT 116 C104(0.89)  LDD0551  [1]
 LDCM0235  AC118 HCT 116 C104(0.90)  LDD0552  [1]
 LDCM0236  AC119 HCT 116 C104(0.86)  LDD0553  [1]
 LDCM0238  AC120 HCT 116 C104(0.80)  LDD0555  [1]
 LDCM0239  AC121 HCT 116 C104(1.06)  LDD0556  [1]
 LDCM0240  AC122 HCT 116 C104(0.91)  LDD0557  [1]
 LDCM0241  AC123 HCT 116 C104(0.99)  LDD0558  [1]
 LDCM0242  AC124 HCT 116 C104(1.13)  LDD0559  [1]
 LDCM0243  AC125 HCT 116 C104(1.10)  LDD0560  [1]
 LDCM0244  AC126 HCT 116 C104(1.06)  LDD0561  [1]
 LDCM0245  AC127 HCT 116 C104(1.03)  LDD0562  [1]
 LDCM0276  AC17 HCT 116 C104(1.02)  LDD0593  [1]
 LDCM0277  AC18 HCT 116 C104(1.02)  LDD0594  [1]
 LDCM0278  AC19 HCT 116 C104(1.11)  LDD0595  [1]
 LDCM0280  AC20 HCT 116 C104(1.13)  LDD0597  [1]
 LDCM0281  AC21 HCT 116 C104(1.04)  LDD0598  [1]
 LDCM0282  AC22 HCT 116 C104(0.94)  LDD0599  [1]
 LDCM0283  AC23 HCT 116 C104(1.01)  LDD0600  [1]
 LDCM0284  AC24 HCT 116 C104(1.15)  LDD0601  [1]
 LDCM0285  AC25 HCT 116 C104(0.93)  LDD0602  [1]
 LDCM0286  AC26 HCT 116 C104(0.95)  LDD0603  [1]
 LDCM0287  AC27 HCT 116 C104(1.03)  LDD0604  [1]
 LDCM0288  AC28 HCT 116 C104(0.90)  LDD0605  [1]
 LDCM0289  AC29 HCT 116 C104(1.08)  LDD0606  [1]
 LDCM0291  AC30 HCT 116 C104(1.03)  LDD0608  [1]
 LDCM0292  AC31 HCT 116 C104(0.98)  LDD0609  [1]
 LDCM0293  AC32 HCT 116 C104(1.03)  LDD0610  [1]
 LDCM0294  AC33 HCT 116 C104(1.03)  LDD0611  [1]
 LDCM0295  AC34 HCT 116 C104(0.99)  LDD0612  [1]
 LDCM0320  AC57 HCT 116 C104(0.92)  LDD0637  [1]
 LDCM0321  AC58 HCT 116 C104(0.90)  LDD0638  [1]
 LDCM0322  AC59 HCT 116 C104(0.97)  LDD0639  [1]
 LDCM0324  AC60 HCT 116 C104(0.81)  LDD0641  [1]
 LDCM0325  AC61 HCT 116 C104(1.09)  LDD0642  [1]
 LDCM0326  AC62 HCT 116 C104(0.86)  LDD0643  [1]
 LDCM0327  AC63 HCT 116 C104(0.96)  LDD0644  [1]
 LDCM0328  AC64 HCT 116 C104(0.97)  LDD0645  [1]
 LDCM0329  AC65 HCT 116 C104(0.98)  LDD0646  [1]
 LDCM0330  AC66 HCT 116 C104(1.06)  LDD0647  [1]
 LDCM0331  AC67 HCT 116 C104(0.88)  LDD0648  [1]
 LDCM0349  AC83 HCT 116 C104(0.88)  LDD0666  [1]
 LDCM0350  AC84 HCT 116 C104(1.02)  LDD0667  [1]
 LDCM0351  AC85 HCT 116 C104(1.06)  LDD0668  [1]
 LDCM0352  AC86 HCT 116 C104(0.96)  LDD0669  [1]
 LDCM0353  AC87 HCT 116 C104(1.10)  LDD0670  [1]
 LDCM0354  AC88 HCT 116 C104(1.13)  LDD0671  [1]
 LDCM0355  AC89 HCT 116 C104(1.00)  LDD0672  [1]
 LDCM0357  AC90 HCT 116 C104(1.11)  LDD0674  [1]
 LDCM0358  AC91 HCT 116 C104(1.01)  LDD0675  [1]
 LDCM0359  AC92 HCT 116 C104(0.95)  LDD0676  [1]
 LDCM0360  AC93 HCT 116 C104(1.03)  LDD0677  [1]
 LDCM0361  AC94 HCT 116 C104(1.05)  LDD0678  [1]
 LDCM0362  AC95 HCT 116 C104(0.88)  LDD0679  [1]
 LDCM0363  AC96 HCT 116 C104(0.97)  LDD0680  [1]
 LDCM0364  AC97 HCT 116 C104(0.99)  LDD0681  [1]
 LDCM0374  CL105 HCT 116 C104(1.06)  LDD0691  [1]
 LDCM0375  CL106 HCT 116 C104(0.98)  LDD0692  [1]
 LDCM0376  CL107 HCT 116 C104(0.94)  LDD0693  [1]
 LDCM0377  CL108 HCT 116 C104(1.02)  LDD0694  [1]
 LDCM0378  CL109 HCT 116 C104(1.09)  LDD0695  [1]
 LDCM0380  CL110 HCT 116 C104(1.26)  LDD0697  [1]
 LDCM0381  CL111 HCT 116 C104(1.23)  LDD0698  [1]
 LDCM0382  CL112 HCT 116 C104(1.05)  LDD0699  [1]
 LDCM0383  CL113 HCT 116 C104(1.01)  LDD0700  [1]
 LDCM0384  CL114 HCT 116 C104(1.03)  LDD0701  [1]
 LDCM0385  CL115 HCT 116 C104(0.97)  LDD0702  [1]
 LDCM0386  CL116 HCT 116 C104(0.98)  LDD0703  [1]
 LDCM0396  CL125 HCT 116 C104(0.89)  LDD0713  [1]
 LDCM0397  CL126 HCT 116 C104(0.91)  LDD0714  [1]
 LDCM0398  CL127 HCT 116 C104(0.90)  LDD0715  [1]
 LDCM0399  CL128 HCT 116 C104(1.06)  LDD0716  [1]
 LDCM0403  CL16 HCT 116 C104(0.73)  LDD0720  [1]
 LDCM0404  CL17 HCT 116 C104(0.85)  LDD0721  [1]
 LDCM0405  CL18 HCT 116 C104(0.99)  LDD0722  [1]
 LDCM0406  CL19 HCT 116 C104(0.99)  LDD0723  [1]
 LDCM0408  CL20 HCT 116 C104(0.87)  LDD0725  [1]
 LDCM0409  CL21 HCT 116 C104(0.86)  LDD0726  [1]
 LDCM0410  CL22 HCT 116 C104(0.93)  LDD0727  [1]
 LDCM0411  CL23 HCT 116 C104(0.89)  LDD0728  [1]
 LDCM0412  CL24 HCT 116 C104(0.88)  LDD0729  [1]
 LDCM0413  CL25 HCT 116 C104(0.88)  LDD0730  [1]
 LDCM0414  CL26 HCT 116 C104(0.97)  LDD0731  [1]
 LDCM0415  CL27 HCT 116 C104(0.90)  LDD0732  [1]
 LDCM0416  CL28 HCT 116 C104(0.93)  LDD0733  [1]
 LDCM0417  CL29 HCT 116 C104(0.88)  LDD0734  [1]
 LDCM0419  CL30 HCT 116 C104(1.07)  LDD0736  [1]
 LDCM0420  CL31 HCT 116 C104(1.05)  LDD0737  [1]
 LDCM0436  CL46 HCT 116 C104(2.17)  LDD0753  [1]
 LDCM0437  CL47 HCT 116 C104(1.39)  LDD0754  [1]
 LDCM0438  CL48 HCT 116 C104(1.30)  LDD0755  [1]
 LDCM0439  CL49 HCT 116 C104(1.74)  LDD0756  [1]
 LDCM0441  CL50 HCT 116 C104(0.95)  LDD0758  [1]
 LDCM0442  CL51 HCT 116 C104(1.51)  LDD0759  [1]
 LDCM0443  CL52 HCT 116 C104(1.80)  LDD0760  [1]
 LDCM0444  CL53 HCT 116 C104(0.61)  LDD0761  [1]
 LDCM0445  CL54 HCT 116 C104(1.47)  LDD0762  [1]
 LDCM0446  CL55 HCT 116 C104(1.26)  LDD0763  [1]
 LDCM0447  CL56 HCT 116 C104(0.98)  LDD0764  [1]
 LDCM0448  CL57 HCT 116 C104(0.89)  LDD0765  [1]
 LDCM0449  CL58 HCT 116 C104(1.43)  LDD0766  [1]
 LDCM0450  CL59 HCT 116 C104(1.27)  LDD0767  [1]
 LDCM0452  CL60 HCT 116 C104(1.60)  LDD0769  [1]
 LDCM0469  CL76 HCT 116 C104(0.58)  LDD0786  [1]
 LDCM0470  CL77 HCT 116 C104(0.51)  LDD0787  [1]
 LDCM0471  CL78 HCT 116 C104(0.73)  LDD0788  [1]
 LDCM0472  CL79 HCT 116 C104(0.76)  LDD0789  [1]
 LDCM0474  CL80 HCT 116 C104(0.71)  LDD0791  [1]
 LDCM0475  CL81 HCT 116 C104(0.75)  LDD0792  [1]
 LDCM0476  CL82 HCT 116 C104(0.86)  LDD0793  [1]
 LDCM0477  CL83 HCT 116 C104(0.72)  LDD0794  [1]
 LDCM0478  CL84 HCT 116 C104(0.79)  LDD0795  [1]
 LDCM0479  CL85 HCT 116 C104(0.73)  LDD0796  [1]
 LDCM0480  CL86 HCT 116 C104(0.63)  LDD0797  [1]
 LDCM0481  CL87 HCT 116 C104(0.70)  LDD0798  [1]
 LDCM0482  CL88 HCT 116 C104(0.51)  LDD0799  [1]
 LDCM0483  CL89 HCT 116 C104(0.71)  LDD0800  [1]
 LDCM0485  CL90 HCT 116 C104(0.74)  LDD0802  [1]
 LDCM0022  KB02 HEK-293T C478(0.77)  LDD1492  [5]
 LDCM0023  KB03 HEK-293T C478(0.98)  LDD1497  [5]
 LDCM0024  KB05 HEK-293T C478(0.79)  LDD1502  [5]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
E3 ubiquitin-protein ligase Mdm2 (MDM2) MDM2/MDM4 family Q00987
Transcription factor
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Runt-related transcription factor 2 (RUNX2) . Q13950
Other
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Histone H2AX (H2AX) Histone H2A family P16104

References

1 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
2 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
3 Benchmarking Cleavable Biotin Tags for Peptide-Centric Chemoproteomics. J Proteome Res. 2022 May 6;21(5):1349-1358. doi: 10.1021/acs.jproteome.2c00174. Epub 2022 Apr 25.
Mass spectrometry data entry: PXD031019
4 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
5 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402