Details of the Target

General Information of Target

Target ID LDTP16836
Target Name F-box only protein 21 (FBXO21)
Gene Name FBXO21
Gene ID 23014
Synonyms
FBX21; KIAA0875; F-box only protein 21
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MINAQELLTLEDVTVEFTWEEWQLLGPFQKDLYRDVMLEIYSNLLSMGYQVSKPDALSKL
ERGEEPWTMEDERHSRICPENNEVDDHLQDHLENQRMLKSVEQYHEHNAFGNTASQTKSL
CLFRENHDTFELYIKTLKSNLSLVNQNKSCEINNSTKFSGDGKSFLHGNYEELYSAAKFS
VSTKANSTKSQVSKHQRTHEIEKNHVCSECGKAFVKKSQLTDHERVHTGEKPYGCTLCAK
VFSRKSRLNEHQRIHKREKSFICSECGKVFTMKSRLIEHQRTHTGEKPYICNECGKGFPG
KRNLIVHQRNHTGEKSYICSECGKGFTGKSMLIIHQRTHTGEKPYICSECGKGFTTKHYV
IIHQRNHTGEKPYICNECGKGFTMKSRMIEHQRTHTGEKPYICSECGKGFPRKSNLIVHQ
RNHTVEKSYLCSECGKGFTVKSMLIIHQRTHTGEKPYTCSECGKGFPLKSRLIVHQRTHT
GEKPYRCSECGKGFIVNSGLMLHQRTHTGEKPYICNECGKGFAFKSNLVVHQRTHTGEKP
FMCSECGKGFTMKRYLIVHQQIHTEEKSCICSECGRGFAKETELALHKQVHTGEKPYGCN
ECGKGFTMKSRLIVHQRTHTGEKPFVCSECRKAFSSKRNLIVHQRTHNGNKP
Target Bioclass
Other
Function Substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex.
Uniprot ID
O94952
Ensemble ID
ENST00000330622.9
HGNC ID
HGNC:13592

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
AGS SNV: p.D7N .
AN3CA Deletion: p.F144LfsTer9 DBIA    Probe Info 
HT115 Insertion: p.I169NfsTer13 .
KYSE30 Insertion: p.A15_P22dup .
RKO SNV: p.I41F .
SNU869 SNV: p.G35D .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 8 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
STPyne
 Probe Info  		K397(6.69)  LDD0277  [1]
Probe 1
 Probe Info  		Y405(16.95); Y570(13.74)  LDD3495  [2]
BTD
 Probe Info  		C59(0.46)  LDD2089  [3]
IPM
 Probe Info  		C59(13.09)  LDD1701  [3]
DBIA
 Probe Info  		C66(0.81)  LDD0532  [4]
4-Iodoacetamidophenylacetylene
 Probe Info  		C229(0.00); C66(0.00)  LDD0038  [5]
IA-alkyne
 Probe Info  		N.A.  LDD0036  [5]
Lodoacetamide azide
 Probe Info  		N.A.  LDD0037  [5]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0524  2-Cyano-N-(2-morpholin-4-yl-ethyl)-acetamide MDA-MB-231 C59(0.93)  LDD2117  [3]
 LDCM0214  AC1 PaTu 8988t C206(0.94)  LDD1093  [4]
 LDCM0215  AC10 HCT 116 C66(0.81)  LDD0532  [4]
 LDCM0216  AC100 HCT 116 C66(1.15)  LDD0533  [4]
 LDCM0217  AC101 HCT 116 C66(1.17)  LDD0534  [4]
 LDCM0218  AC102 HCT 116 C66(1.17)  LDD0535  [4]
 LDCM0219  AC103 HCT 116 C66(1.08)  LDD0536  [4]
 LDCM0220  AC104 HCT 116 C66(0.89)  LDD0537  [4]
 LDCM0221  AC105 HCT 116 C66(0.92)  LDD0538  [4]
 LDCM0222  AC106 HCT 116 C66(1.27)  LDD0539  [4]
 LDCM0223  AC107 HCT 116 C66(1.02)  LDD0540  [4]
 LDCM0224  AC108 HCT 116 C66(1.16)  LDD0541  [4]
 LDCM0225  AC109 HCT 116 C66(1.33)  LDD0542  [4]
 LDCM0226  AC11 HCT 116 C66(1.16)  LDD0543  [4]
 LDCM0227  AC110 HCT 116 C66(0.98)  LDD0544  [4]
 LDCM0228  AC111 HCT 116 C66(0.99)  LDD0545  [4]
 LDCM0229  AC112 HCT 116 C66(0.95)  LDD0546  [4]
 LDCM0237  AC12 HCT 116 C66(0.77)  LDD0554  [4]
 LDCM0259  AC14 HCT 116 C66(0.83)  LDD0576  [4]
 LDCM0270  AC15 HCT 116 C66(0.63)  LDD0587  [4]
 LDCM0276  AC17 HCT 116 C66(1.15)  LDD0593  [4]
 LDCM0277  AC18 HCT 116 C66(1.23)  LDD0594  [4]
 LDCM0278  AC19 HCT 116 C66(1.29)  LDD0595  [4]
 LDCM0279  AC2 PaTu 8988t C206(0.90)  LDD1158  [4]
 LDCM0280  AC20 HCT 116 C66(1.09)  LDD0597  [4]
 LDCM0281  AC21 HCT 116 C66(1.38)  LDD0598  [4]
 LDCM0282  AC22 HCT 116 C66(1.08)  LDD0599  [4]
 LDCM0283  AC23 HCT 116 C66(1.17)  LDD0600  [4]
 LDCM0284  AC24 HCT 116 C66(1.27)  LDD0601  [4]
 LDCM0285  AC25 PaTu 8988t C229(0.95)  LDD1164  [4]
 LDCM0286  AC26 PaTu 8988t C229(1.06)  LDD1165  [4]
 LDCM0287  AC27 PaTu 8988t C229(0.99)  LDD1166  [4]
 LDCM0288  AC28 PaTu 8988t C229(0.93)  LDD1167  [4]
 LDCM0289  AC29 PaTu 8988t C229(0.90)  LDD1168  [4]
 LDCM0290  AC3 PaTu 8988t C206(0.93)  LDD1169  [4]
 LDCM0291  AC30 PaTu 8988t C229(1.09)  LDD1170  [4]
 LDCM0292  AC31 PaTu 8988t C229(1.13)  LDD1171  [4]
 LDCM0293  AC32 PaTu 8988t C229(0.94)  LDD1172  [4]
 LDCM0294  AC33 PaTu 8988t C229(0.97)  LDD1173  [4]
 LDCM0295  AC34 PaTu 8988t C229(1.14)  LDD1174  [4]
 LDCM0296  AC35 PaTu 8988t C206(1.15)  LDD1175  [4]
 LDCM0297  AC36 PaTu 8988t C206(0.67)  LDD1176  [4]
 LDCM0298  AC37 PaTu 8988t C206(0.61)  LDD1177  [4]
 LDCM0299  AC38 PaTu 8988t C206(0.66)  LDD1178  [4]
 LDCM0300  AC39 PaTu 8988t C206(0.68)  LDD1179  [4]
 LDCM0301  AC4 PaTu 8988t C206(1.05)  LDD1180  [4]
 LDCM0302  AC40 PaTu 8988t C206(0.75)  LDD1181  [4]
 LDCM0303  AC41 PaTu 8988t C206(0.81)  LDD1182  [4]
 LDCM0304  AC42 PaTu 8988t C206(0.59)  LDD1183  [4]
 LDCM0305  AC43 PaTu 8988t C206(0.77)  LDD1184  [4]
 LDCM0306  AC44 PaTu 8988t C206(0.64)  LDD1185  [4]
 LDCM0307  AC45 PaTu 8988t C206(0.59)  LDD1186  [4]
 LDCM0308  AC46 PaTu 8988t C229(0.89)  LDD1187  [4]
 LDCM0309  AC47 PaTu 8988t C229(0.86)  LDD1188  [4]
 LDCM0310  AC48 PaTu 8988t C229(0.82)  LDD1189  [4]
 LDCM0311  AC49 PaTu 8988t C229(0.83)  LDD1190  [4]
 LDCM0312  AC5 PaTu 8988t C206(1.01)  LDD1191  [4]
 LDCM0313  AC50 PaTu 8988t C229(0.85)  LDD1192  [4]
 LDCM0314  AC51 PaTu 8988t C229(0.59)  LDD1193  [4]
 LDCM0315  AC52 PaTu 8988t C229(0.76)  LDD1194  [4]
 LDCM0316  AC53 PaTu 8988t C229(0.82)  LDD1195  [4]
 LDCM0317  AC54 PaTu 8988t C229(0.96)  LDD1196  [4]
 LDCM0318  AC55 PaTu 8988t C229(0.78)  LDD1197  [4]
 LDCM0319  AC56 PaTu 8988t C229(0.80)  LDD1198  [4]
 LDCM0320  AC57 HEK-293T C206(1.30)  LDD1559  [6]
 LDCM0321  AC58 HEK-293T C206(0.88)  LDD1560  [6]
 LDCM0323  AC6 HCT 116 C66(1.06)  LDD0640  [4]
 LDCM0334  AC7 HCT 116 C66(0.74)  LDD0651  [4]
 LDCM0345  AC8 HCT 116 C66(1.02)  LDD0662  [4]
 LDCM0365  AC98 HCT 116 C66(0.98)  LDD0682  [4]
 LDCM0366  AC99 HCT 116 C66(1.05)  LDD0683  [4]
 LDCM0545  Acetamide MDA-MB-231 C59(0.90)  LDD2138  [3]
 LDCM0248  AKOS034007472 HCT 116 C66(0.89)  LDD0565  [4]
 LDCM0356  AKOS034007680 HCT 116 C66(0.73)  LDD0673  [4]
 LDCM0275  AKOS034007705 HCT 116 C66(0.83)  LDD0592  [4]
 LDCM0369  CL100 PaTu 8988t C206(0.70)  LDD1248  [4]
 LDCM0370  CL101 HCT 116 C66(0.93)  LDD0687  [4]
 LDCM0371  CL102 HCT 116 C66(0.99)  LDD0688  [4]
 LDCM0372  CL103 HCT 116 C66(0.85)  LDD0689  [4]
 LDCM0373  CL104 HCT 116 C66(1.12)  LDD0690  [4]
 LDCM0374  CL105 HCT 116 C66(1.16)  LDD0691  [4]
 LDCM0375  CL106 HCT 116 C66(1.38)  LDD0692  [4]
 LDCM0376  CL107 HCT 116 C66(0.99)  LDD0693  [4]
 LDCM0377  CL108 HCT 116 C66(1.09)  LDD0694  [4]
 LDCM0378  CL109 HCT 116 C66(1.33)  LDD0695  [4]
 LDCM0380  CL110 HCT 116 C66(1.10)  LDD0697  [4]
 LDCM0381  CL111 HCT 116 C66(1.29)  LDD0698  [4]
 LDCM0382  CL112 PaTu 8988t C229(0.77)  LDD1261  [4]
 LDCM0383  CL113 PaTu 8988t C229(0.91)  LDD1262  [4]
 LDCM0384  CL114 PaTu 8988t C229(0.90)  LDD1263  [4]
 LDCM0385  CL115 PaTu 8988t C229(1.02)  LDD1264  [4]
 LDCM0386  CL116 PaTu 8988t C229(0.81)  LDD1265  [4]
 LDCM0387  CL117 PaTu 8988t C206(0.73)  LDD1266  [4]
 LDCM0388  CL118 PaTu 8988t C206(0.49)  LDD1267  [4]
 LDCM0389  CL119 PaTu 8988t C206(0.64)  LDD1268  [4]
 LDCM0391  CL120 PaTu 8988t C206(0.67)  LDD1270  [4]
 LDCM0392  CL121 PaTu 8988t C229(0.91)  LDD1271  [4]
 LDCM0393  CL122 PaTu 8988t C229(0.87)  LDD1272  [4]
 LDCM0394  CL123 PaTu 8988t C229(0.98)  LDD1273  [4]
 LDCM0395  CL124 PaTu 8988t C229(0.84)  LDD1274  [4]
 LDCM0399  CL128 HEK-293T C66(1.09)  LDD1603  [6]
 LDCM0403  CL16 HEK-293T C66(0.97)  LDD1001  [4]
 LDCM0404  CL17 HCT 116 C66(1.41)  LDD0721  [4]
 LDCM0405  CL18 HCT 116 C66(1.03)  LDD0722  [4]
 LDCM0406  CL19 HCT 116 C66(1.47)  LDD0723  [4]
 LDCM0408  CL20 HCT 116 C66(0.85)  LDD0725  [4]
 LDCM0409  CL21 HCT 116 C66(1.17)  LDD0726  [4]
 LDCM0410  CL22 HCT 116 C66(0.87)  LDD0727  [4]
 LDCM0411  CL23 HCT 116 C66(0.55)  LDD0728  [4]
 LDCM0412  CL24 HCT 116 C66(0.60)  LDD0729  [4]
 LDCM0413  CL25 HCT 116 C66(0.58)  LDD0730  [4]
 LDCM0414  CL26 HCT 116 C66(0.78)  LDD0731  [4]
 LDCM0415  CL27 HCT 116 C66(0.73)  LDD0732  [4]
 LDCM0416  CL28 HCT 116 C66(0.75)  LDD0733  [4]
 LDCM0417  CL29 HCT 116 C66(0.60)  LDD0734  [4]
 LDCM0419  CL30 HCT 116 C66(0.50)  LDD0736  [4]
 LDCM0420  CL31 HCT 116 C66(0.92)  LDD0737  [4]
 LDCM0421  CL32 HEK-293T C66(0.66)  LDD1019  [4]
 LDCM0422  CL33 HEK-293T C66(0.84)  LDD1020  [4]
 LDCM0423  CL34 HEK-293T C66(0.68)  LDD1021  [4]
 LDCM0424  CL35 HEK-293T C66(0.56)  LDD1022  [4]
 LDCM0425  CL36 HEK-293T C66(0.79)  LDD1023  [4]
 LDCM0426  CL37 HEK-293T C66(0.82)  LDD1024  [4]
 LDCM0428  CL39 HEK-293T C66(0.48)  LDD1026  [4]
 LDCM0429  CL4 HEK-293T C66(1.15)  LDD1633  [6]
 LDCM0430  CL40 HEK-293T C66(0.64)  LDD1028  [4]
 LDCM0431  CL41 HEK-293T C66(0.54)  LDD1029  [4]
 LDCM0432  CL42 HEK-293T C66(0.73)  LDD1030  [4]
 LDCM0433  CL43 HEK-293T C66(0.77)  LDD1031  [4]
 LDCM0434  CL44 HEK-293T C66(0.83)  LDD1032  [4]
 LDCM0435  CL45 HEK-293T C66(0.48)  LDD1033  [4]
 LDCM0436  CL46 HCT 116 C66(1.02)  LDD0753  [4]
 LDCM0437  CL47 HCT 116 C66(0.75)  LDD0754  [4]
 LDCM0438  CL48 HCT 116 C66(0.93)  LDD0755  [4]
 LDCM0439  CL49 HCT 116 C66(1.12)  LDD0756  [4]
 LDCM0440  CL5 HEK-293T C206(1.16)  LDD1644  [6]
 LDCM0441  CL50 HCT 116 C66(1.08)  LDD0758  [4]
 LDCM0442  CL51 HCT 116 C66(1.00)  LDD0759  [4]
 LDCM0443  CL52 HCT 116 C66(1.00)  LDD0760  [4]
 LDCM0444  CL53 HCT 116 C66(0.72)  LDD0761  [4]
 LDCM0445  CL54 HCT 116 C66(0.97)  LDD0762  [4]
 LDCM0446  CL55 HCT 116 C66(0.95)  LDD0763  [4]
 LDCM0447  CL56 HCT 116 C66(0.82)  LDD0764  [4]
 LDCM0448  CL57 HCT 116 C66(0.87)  LDD0765  [4]
 LDCM0449  CL58 HCT 116 C66(1.01)  LDD0766  [4]
 LDCM0450  CL59 HCT 116 C66(1.07)  LDD0767  [4]
 LDCM0451  CL6 HEK-293T C206(0.66)  LDD1654  [6]
 LDCM0452  CL60 HCT 116 C66(1.00)  LDD0769  [4]
 LDCM0453  CL61 HEK-293T C66(1.00)  LDD1051  [4]
 LDCM0454  CL62 HEK-293T C66(0.85)  LDD1052  [4]
 LDCM0455  CL63 HEK-293T C66(0.54)  LDD1053  [4]
 LDCM0456  CL64 HEK-293T C66(1.84)  LDD1054  [4]
 LDCM0457  CL65 HEK-293T C66(0.59)  LDD1055  [4]
 LDCM0458  CL66 HEK-293T C66(0.75)  LDD1056  [4]
 LDCM0459  CL67 HEK-293T C66(1.30)  LDD1057  [4]
 LDCM0460  CL68 HEK-293T C66(1.04)  LDD1058  [4]
 LDCM0461  CL69 HEK-293T C66(0.79)  LDD1059  [4]
 LDCM0463  CL70 HEK-293T C66(0.83)  LDD1061  [4]
 LDCM0464  CL71 HEK-293T C66(0.95)  LDD1062  [4]
 LDCM0465  CL72 HEK-293T C66(1.37)  LDD1063  [4]
 LDCM0466  CL73 HEK-293T C66(0.92)  LDD1064  [4]
 LDCM0467  CL74 HEK-293T C66(1.04)  LDD1065  [4]
 LDCM0469  CL76 PaTu 8988t C229(0.77)  LDD1348  [4]
 LDCM0470  CL77 PaTu 8988t C229(0.77)  LDD1349  [4]
 LDCM0471  CL78 PaTu 8988t C229(0.68)  LDD1350  [4]
 LDCM0472  CL79 PaTu 8988t C229(0.68)  LDD1351  [4]
 LDCM0474  CL80 PaTu 8988t C229(0.74)  LDD1353  [4]
 LDCM0475  CL81 PaTu 8988t C229(0.64)  LDD1354  [4]
 LDCM0476  CL82 PaTu 8988t C229(0.67)  LDD1355  [4]
 LDCM0477  CL83 PaTu 8988t C229(0.59)  LDD1356  [4]
 LDCM0478  CL84 PaTu 8988t C229(0.75)  LDD1357  [4]
 LDCM0479  CL85 PaTu 8988t C229(0.62)  LDD1358  [4]
 LDCM0480  CL86 PaTu 8988t C229(0.54)  LDD1359  [4]
 LDCM0481  CL87 PaTu 8988t C229(0.55)  LDD1360  [4]
 LDCM0482  CL88 PaTu 8988t C229(0.73)  LDD1361  [4]
 LDCM0483  CL89 PaTu 8988t C229(0.88)  LDD1362  [4]
 LDCM0485  CL90 PaTu 8988t C229(0.67)  LDD1364  [4]
 LDCM0486  CL91 PaTu 8988t C206(1.19)  LDD1365  [4]
 LDCM0487  CL92 PaTu 8988t C206(0.89)  LDD1366  [4]
 LDCM0488  CL93 PaTu 8988t C206(0.71)  LDD1367  [4]
 LDCM0489  CL94 PaTu 8988t C206(1.00)  LDD1368  [4]
 LDCM0490  CL95 PaTu 8988t C206(0.97)  LDD1369  [4]
 LDCM0491  CL96 PaTu 8988t C206(0.87)  LDD1370  [4]
 LDCM0492  CL97 PaTu 8988t C206(0.84)  LDD1371  [4]
 LDCM0493  CL98 PaTu 8988t C206(0.81)  LDD1372  [4]
 LDCM0494  CL99 PaTu 8988t C206(0.96)  LDD1373  [4]
 LDCM0213  Electrophilic fragment 2 MDA-MB-231 C59(4.44)  LDD1702  [3]
 LDCM0468  Fragment33 HEK-293T C66(0.99)  LDD1066  [4]
 LDCM0427  Fragment51 HEK-293T C66(0.79)  LDD1025  [4]
 LDCM0022  KB02 A498 C66(3.44)  LDD2259  [7]
 LDCM0023  KB03 MDA-MB-231 C59(13.09)  LDD1701  [3]
 LDCM0024  KB05 G361 C59(5.15)  LDD3311  [7]
 LDCM0496  Nucleophilic fragment 11a MDA-MB-231 C59(0.46)  LDD2089  [3]
 LDCM0506  Nucleophilic fragment 16a MDA-MB-231 C59(0.89)  LDD2099  [3]
 LDCM0514  Nucleophilic fragment 20a MDA-MB-231 C59(0.90)  LDD2107  [3]
 LDCM0530  Nucleophilic fragment 28a MDA-MB-231 C59(0.85)  LDD2123  [3]
 LDCM0532  Nucleophilic fragment 29a MDA-MB-231 C59(1.09)  LDD2125  [3]
 LDCM0534  Nucleophilic fragment 30a MDA-MB-231 C59(2.22)  LDD2127  [3]
 LDCM0543  Nucleophilic fragment 38 MDA-MB-231 C59(0.92)  LDD2136  [3]
 LDCM0544  Nucleophilic fragment 39 MDA-MB-231 C59(0.72)  LDD2137  [3]
 LDCM0546  Nucleophilic fragment 40 MDA-MB-231 C59(0.80)  LDD2140  [3]

References

1 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
2 An Azo Coupling-Based Chemoproteomic Approach to Systematically Profile the Tyrosine Reactivity in the Human Proteome. Anal Chem. 2021 Jul 27;93(29):10334-10342. doi: 10.1021/acs.analchem.1c01935. Epub 2021 Jul 12.
3 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
4 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
5 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
6 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
7 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840