Details of the Target

General Information of Target

Target ID LDTP16261
Target Name PALM2-AKAP2 fusion protein (PALM2AKAP2)
Gene Name PALM2AKAP2
Gene ID 445815
Synonyms
KIAA0920; PAKAP; PALM2; PALM2-AKAP2 fusion protein; A-kinase anchor protein 2; AKAP-2; AKAP-KL; Paralemmin A kinase anchor protein; Paralemmin-2; Protein kinase A-anchoring protein 2; PRKA2
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MVVMNSLRVILQASPGKLLWRKFQIPRFMPARPCSLYTCTYKTRNRALHPLWESVDLVPG
GDRQSPINIRWRDSVYDPGLKPLTISYDPATCLHVWNNGYSFLVEFEDSTDKSVIKGGPL
EHNYRLKQFHFHWGAIDAWGSEHTVDSKCFPAELHLVHWNAVRFENFEDAALEENGLAVI
GVFLKLGKHHKELQKLVDTLPSIKHKDALVEFGSFDPSCLMPTCPDYWTYSGSLTTPPLS
ESVTWIIKKQPVEVDHDQLEQFRTLLFTSEGEKEKRMVDNFRPLQPLMNRTVRSSFRHDY
VLNVQAKPKPATSQATP
Target Bioclass
Other
Function
Binds to regulatory subunit (RII) of protein kinase A. May be involved in establishing polarity in signaling systems or in integrating PKA-RII isoforms with downstream effectors to capture, amplify and focus diffuse, trans-cellular signals carried by cAMP. Binds to and modulates the structure of the actin cytoskeleton.
Uniprot ID
Q9Y2D5
Ensemble ID
ENST00000259318.7
HGNC ID
HGNC:33529

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 17 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
m-APA
 Probe Info  		15.00  LDD0402  [1]
CY-1
 Probe Info  		100.00  LDD0243  [2]
STPyne
 Probe Info  		K174(7.69); K178(8.14); K188(7.58); K189(5.85)  LDD0277  [3]
DBIA
 Probe Info  		C148(1.50)  LDD3401  [4]
BTD
 Probe Info  		C296(1.48)  LDD2118  [5]
Johansson_61
 Probe Info  		_(20.00)  LDD1485  [6]
DA-P3
 Probe Info  		7.68  LDD0180  [7]
HHS-475
 Probe Info  		Y680(1.38)  LDD0264  [8]
Lodoacetamide azide
 Probe Info  		N.A.  LDD0037  [9]
IPM
 Probe Info  		C205(0.00); C296(0.00); C161(0.00)  LDD0025  [10]
TFBX
 Probe Info  		N.A.  LDD0027  [10]
IA-alkyne
 Probe Info  		N.A.  LDD0151  [11]
VSF
 Probe Info  		N.A.  LDD0007  [12]
Phosphinate-6
 Probe Info  		C762(0.00); C205(0.00); C296(0.00)  LDD0018  [13]
Acrolein
 Probe Info  		N.A.  LDD0217  [14]
Methacrolein
 Probe Info  		N.A.  LDD0218  [14]
AOyne
 Probe Info  		12.90  LDD0443  [15]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0156  Aniline NCI-H1299 11.65  LDD0403  [1]
 LDCM0108  Chloroacetamide HeLa N.A.  LDD0222  [14]
 LDCM0028  Dobutamine HEK-293T 7.68  LDD0180  [7]
 LDCM0213  Electrophilic fragment 2 MDA-MB-231 C205(1.30); C296(1.08)  LDD1702  [5]
 LDCM0031  Epigallocatechin gallate HEK-293T 5.57  LDD0183  [7]
 LDCM0625  F8 Ramos C296(1.85); C205(2.19); C161(1.68)  LDD2187  [16]
 LDCM0572  Fragment10 Ramos C296(1.80); C161(2.25)  LDD2189  [16]
 LDCM0573  Fragment11 Ramos C296(0.36); C205(0.90); C161(0.20)  LDD2190  [16]
 LDCM0574  Fragment12 Ramos C296(0.94); C205(0.89); C161(0.52)  LDD2191  [16]
 LDCM0575  Fragment13 Ramos C296(0.77); C161(0.65)  LDD2192  [16]
 LDCM0576  Fragment14 Ramos C296(0.36); C205(0.97); C161(0.54)  LDD2193  [16]
 LDCM0579  Fragment20 Ramos C296(0.69); C205(0.81); C161(1.12)  LDD2194  [16]
 LDCM0580  Fragment21 Ramos C296(0.81); C205(1.06); C161(1.02)  LDD2195  [16]
 LDCM0582  Fragment23 Ramos C296(1.17)  LDD2196  [16]
 LDCM0578  Fragment27 Ramos C296(1.02)  LDD2197  [16]
 LDCM0586  Fragment28 Ramos C296(0.54); C205(0.56); C161(1.05)  LDD2198  [16]
 LDCM0588  Fragment30 Ramos C296(0.95); C205(0.91); C161(1.46)  LDD2199  [16]
 LDCM0589  Fragment31 Ramos C296(1.17); C205(1.22); C161(2.12)  LDD2200  [16]
 LDCM0590  Fragment32 Ramos C296(3.23)  LDD2201  [16]
 LDCM0468  Fragment33 Ramos C296(1.44); C205(2.02); C161(0.92)  LDD2202  [16]
 LDCM0596  Fragment38 Ramos C296(1.23); C161(1.09)  LDD2203  [16]
 LDCM0566  Fragment4 Ramos C296(1.14); C205(0.91); C161(1.05)  LDD2184  [16]
 LDCM0610  Fragment52 Ramos C296(1.01); C161(0.69)  LDD2204  [16]
 LDCM0614  Fragment56 Ramos C296(1.33); C205(1.44); C161(0.89)  LDD2205  [16]
 LDCM0569  Fragment7 Jurkat _(20.00)  LDD1485  [6]
 LDCM0571  Fragment9 Ramos C296(0.83); C205(0.73); C161(1.26)  LDD2188  [16]
 LDCM0116  HHS-0101 DM93 Y680(1.38)  LDD0264  [8]
 LDCM0117  HHS-0201 DM93 Y680(3.79)  LDD0265  [8]
 LDCM0118  HHS-0301 DM93 Y680(4.06)  LDD0266  [8]
 LDCM0119  HHS-0401 DM93 Y680(2.61)  LDD0267  [8]
 LDCM0120  HHS-0701 DM93 Y680(1.67)  LDD0268  [8]
 LDCM0107  IAA HeLa N.A.  LDD0221  [14]
 LDCM0022  KB02 Ramos C296(1.76); C205(1.88)  LDD2182  [16]
 LDCM0023  KB03 MDA-MB-231 C205(1.96)  LDD1701  [5]
 LDCM0024  KB05 RD C148(1.50)  LDD3401  [4]
 LDCM0109  NEM HeLa N.A.  LDD0223  [14]
 LDCM0525  Nucleophilic fragment 25b MDA-MB-231 C296(1.48)  LDD2118  [5]
 LDCM0531  Nucleophilic fragment 28b MDA-MB-231 C296(1.66)  LDD2124  [5]
 LDCM0547  Nucleophilic fragment 41 MDA-MB-231 C296(0.80)  LDD2141  [5]
 LDCM0556  Nucleophilic fragment 8a MDA-MB-231 C296(0.58)  LDD2150  [5]
 LDCM0029  Quercetin HEK-293T 8.74  LDD0181  [7]
 LDCM0170  Structure8 Ramos 6.46  LDD0433  [17]

References

1 Quantitative and Site-Specific Chemoproteomic Profiling of Targets of Acrolein. Chem Res Toxicol. 2019 Mar 18;32(3):467-473. doi: 10.1021/acs.chemrestox.8b00343. Epub 2019 Jan 15.
2 Cyclopropenone, Cyclopropeniminium Ion, and Cyclopropenethione as Novel Electrophilic Warheads for Potential Target Discovery of Triple-Negative Breast Cancer. J Med Chem. 2023 Feb 23;66(4):2851-2864. doi: 10.1021/acs.jmedchem.2c01889. Epub 2023 Feb 10.
3 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
4 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
5 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
6 Proteome-wide covalent ligand discovery in native biological systems. Nature. 2016 Jun 23;534(7608):570-4. doi: 10.1038/nature18002. Epub 2016 Jun 15.
7 A chemical probe unravels the reactive proteome of health-associated catechols. Chem Sci. 2023 Jul 22;14(32):8635-8643. doi: 10.1039/d3sc00888f. eCollection 2023 Aug 16.
Mass spectrometry data entry: PXD043348
8 Discovery of a Cell-Active SuTEx Ligand of Prostaglandin Reductase 2. Chembiochem. 2021 Jun 15;22(12):2134-2139. doi: 10.1002/cbic.202000879. Epub 2021 Apr 29.
9 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
10 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
11 Sequence-Based Prediction of Cysteine Reactivity Using Machine Learning. Biochemistry. 2018 Jan 30;57(4):451-460. doi: 10.1021/acs.biochem.7b00897. Epub 2017 Oct 26.
12 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
13 DFT-Guided Discovery of Ethynyl-Triazolyl-Phosphinates as Modular Electrophiles for Chemoselective Cysteine Bioconjugation and Profiling. Angew Chem Int Ed Engl. 2022 Oct 10;61(41):e202205348. doi: 10.1002/anie.202205348. Epub 2022 Aug 22.
Mass spectrometry data entry: PXD033004
14 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
15 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.
16 Site-specific quantitative cysteine profiling with data-independent acquisition-based mass spectrometry. Methods Enzymol. 2023;679:295-322. doi: 10.1016/bs.mie.2022.07.037. Epub 2022 Sep 7.
Mass spectrometry data entry: PXD027578
17 2-Sulfonylpyridines as Tunable, Cysteine-Reactive Electrophiles. J Am Chem Soc. 2020 May 13;142(19):8972-8979. doi: 10.1021/jacs.0c02721. Epub 2020 Apr 29.