Details of the Target

General Information of Target

Target ID LDTP15917
Target Name Large ribosomal subunit protein uL4m (MRPL4)
Gene Name MRPL4
Gene ID 51073
Synonyms
Large ribosomal subunit protein uL4m; 39S ribosomal protein L4, mitochondrial; L4mt; MRP-L4
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MAAPVVTAPGRALLRAGAGRLLRGGVQELLRPRHEGNAPDLACNFSLSQNRGTVIVERWW
KVPLAGEGRKPRLHRRHRVYKLVEDTKHRPKENLELILTQSVENVGVRGDLVSVKKSLGR
NRLLPQGLAVYASPENKKLFEEEKLLRQEGKLEKIQTKAGEATVKFLKSCRLEVGMKNNV
KWELNPEIVARHFFKNLGVVVAPHTLKLPEEPITRWGEYWCEVTVNGLDTVRVPMSVVNF
EKPKTKRYKYWLAQQAAKAMAPTSPQI
Target Bioclass
Other
Family
Universal ribosomal protein uL4 family
Subcellular location
Mitochondrion
Uniprot ID
Q9BYD3
Ensemble ID
ENST00000253099.11
HGNC ID
HGNC:14276

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
CAOV3 SNV: p.A233S .
CCK81 SNV: p.Y209C .
LS180 SNV: p.E33Ter .
SKMEL28 SNV: p.P84S .
SNU1 SNV: p.Q302R .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 12 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
m-APA
 Probe Info  		12.89  LDD0402  [1]
N1
 Probe Info  		100.00  LDD0242  [2]
C-Sul
 Probe Info  		5.69  LDD0066  [3]
DBIA
 Probe Info  		C311(1.47)  LDD3439  [4]
BTD
 Probe Info  		C311(0.91)  LDD2094  [5]
Acrolein
 Probe Info  		N.A.  LDD0225  [6]
IA-alkyne
 Probe Info  		N.A.  LDD0162  [7]
IPM
 Probe Info  		N.A.  LDD0147  [8]
TFBX
 Probe Info  		N.A.  LDD0148  [8]
Ox-W18
 Probe Info  		N.A.  LDD2175  [9]
AOyne
 Probe Info  		15.00  LDD0443  [10]
NAIA_5
 Probe Info  		N.A.  LDD2223  [11]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0548  1-(4-(Benzo[d][1,3]dioxol-5-ylmethyl)piperazin-1-yl)-2-nitroethan-1-one MDA-MB-231 C311(0.62)  LDD2142  [5]
 LDCM0215  AC10 HEK-293T C311(1.13)  LDD1508  [12]
 LDCM0259  AC14 HEK-293T C311(1.07)  LDD1512  [12]
 LDCM0270  AC15 HEK-293T C311(1.05)  LDD1513  [12]
 LDCM0277  AC18 HEK-293T C311(1.02)  LDD1516  [12]
 LDCM0279  AC2 HEK-293T C311(1.01)  LDD1518  [12]
 LDCM0281  AC21 HEK-293T C311(0.86)  LDD1520  [12]
 LDCM0282  AC22 HEK-293T C311(1.12)  LDD1521  [12]
 LDCM0283  AC23 HEK-293T C311(1.09)  LDD1522  [12]
 LDCM0284  AC24 HEK-293T C311(0.97)  LDD1523  [12]
 LDCM0286  AC26 HEK-293T C311(1.02)  LDD1525  [12]
 LDCM0289  AC29 HEK-293T C311(1.08)  LDD1528  [12]
 LDCM0291  AC30 HEK-293T C311(0.99)  LDD1530  [12]
 LDCM0292  AC31 HEK-293T C311(0.95)  LDD1531  [12]
 LDCM0293  AC32 HEK-293T C311(1.02)  LDD1532  [12]
 LDCM0295  AC34 HEK-293T C311(1.15)  LDD1534  [12]
 LDCM0298  AC37 HEK-293T C311(0.98)  LDD1537  [12]
 LDCM0299  AC38 HEK-293T C311(1.02)  LDD1538  [12]
 LDCM0300  AC39 HEK-293T C311(1.21)  LDD1539  [12]
 LDCM0302  AC40 HEK-293T C311(1.30)  LDD1541  [12]
 LDCM0304  AC42 HEK-293T C311(1.17)  LDD1543  [12]
 LDCM0307  AC45 HEK-293T C311(0.94)  LDD1546  [12]
 LDCM0308  AC46 HEK-293T C311(1.03)  LDD1547  [12]
 LDCM0309  AC47 HEK-293T C311(1.13)  LDD1548  [12]
 LDCM0310  AC48 HEK-293T C311(0.99)  LDD1549  [12]
 LDCM0312  AC5 HEK-293T C311(1.00)  LDD1551  [12]
 LDCM0313  AC50 HEK-293T C311(1.15)  LDD1552  [12]
 LDCM0316  AC53 HEK-293T C311(1.01)  LDD1555  [12]
 LDCM0317  AC54 HEK-293T C311(1.06)  LDD1556  [12]
 LDCM0318  AC55 HEK-293T C311(1.00)  LDD1557  [12]
 LDCM0319  AC56 HEK-293T C311(1.01)  LDD1558  [12]
 LDCM0321  AC58 HEK-293T C311(1.06)  LDD1560  [12]
 LDCM0323  AC6 HEK-293T C311(0.88)  LDD1562  [12]
 LDCM0325  AC61 HEK-293T C311(0.94)  LDD1564  [12]
 LDCM0326  AC62 HEK-293T C311(0.92)  LDD1565  [12]
 LDCM0327  AC63 HEK-293T C311(0.97)  LDD1566  [12]
 LDCM0328  AC64 HEK-293T C311(0.89)  LDD1567  [12]
 LDCM0334  AC7 HEK-293T C311(1.17)  LDD1568  [12]
 LDCM0345  AC8 HEK-293T C311(1.04)  LDD1569  [12]
 LDCM0248  AKOS034007472 HEK-293T C311(1.01)  LDD1511  [12]
 LDCM0275  AKOS034007705 HEK-293T C311(0.92)  LDD1514  [12]
 LDCM0156  Aniline NCI-H1299 12.05  LDD0403  [1]
 LDCM0367  CL1 HEK-293T C311(1.18)  LDD1571  [12]
 LDCM0368  CL10 HEK-293T C311(1.11)  LDD1572  [12]
 LDCM0370  CL101 HEK-293T C311(0.97)  LDD1574  [12]
 LDCM0372  CL103 HEK-293T C311(0.97)  LDD1576  [12]
 LDCM0374  CL105 HEK-293T C311(0.95)  LDD1578  [12]
 LDCM0376  CL107 HEK-293T C311(0.94)  LDD1580  [12]
 LDCM0378  CL109 HEK-293T C311(1.01)  LDD1582  [12]
 LDCM0379  CL11 HEK-293T C311(1.22)  LDD1583  [12]
 LDCM0381  CL111 HEK-293T C311(1.00)  LDD1585  [12]
 LDCM0383  CL113 HEK-293T C311(1.04)  LDD1587  [12]
 LDCM0385  CL115 HEK-293T C311(0.93)  LDD1589  [12]
 LDCM0387  CL117 HEK-293T C311(1.02)  LDD1591  [12]
 LDCM0389  CL119 HEK-293T C311(0.98)  LDD1593  [12]
 LDCM0390  CL12 HEK-293T C311(1.23)  LDD1594  [12]
 LDCM0392  CL121 HEK-293T C311(0.99)  LDD1596  [12]
 LDCM0394  CL123 HEK-293T C311(0.91)  LDD1598  [12]
 LDCM0396  CL125 HEK-293T C311(0.80)  LDD1600  [12]
 LDCM0398  CL127 HEK-293T C311(0.90)  LDD1602  [12]
 LDCM0400  CL13 HEK-293T C311(1.06)  LDD1604  [12]
 LDCM0402  CL15 HEK-293T C311(0.98)  LDD1606  [12]
 LDCM0405  CL18 HEK-293T C311(1.24)  LDD1609  [12]
 LDCM0409  CL21 HEK-293T C311(1.31)  LDD1613  [12]
 LDCM0410  CL22 HEK-293T C311(1.18)  LDD1614  [12]
 LDCM0411  CL23 HEK-293T C311(1.37)  LDD1615  [12]
 LDCM0412  CL24 HEK-293T C311(1.32)  LDD1616  [12]
 LDCM0413  CL25 HEK-293T C311(0.84)  LDD1617  [12]
 LDCM0415  CL27 HEK-293T C311(0.91)  LDD1619  [12]
 LDCM0418  CL3 HEK-293T C311(1.05)  LDD1622  [12]
 LDCM0419  CL30 HEK-293T C311(1.15)  LDD1623  [12]
 LDCM0422  CL33 HEK-293T C311(0.97)  LDD1626  [12]
 LDCM0423  CL34 HEK-293T C311(1.02)  LDD1627  [12]
 LDCM0424  CL35 HEK-293T C311(1.22)  LDD1628  [12]
 LDCM0425  CL36 HEK-293T C311(1.11)  LDD1629  [12]
 LDCM0426  CL37 HEK-293T C311(0.96)  LDD1630  [12]
 LDCM0428  CL39 HEK-293T C311(1.03)  LDD1632  [12]
 LDCM0432  CL42 HEK-293T C311(1.18)  LDD1636  [12]
 LDCM0435  CL45 HEK-293T C311(1.09)  LDD1639  [12]
 LDCM0436  CL46 HEK-293T C311(1.14)  LDD1640  [12]
 LDCM0437  CL47 HEK-293T C311(1.26)  LDD1641  [12]
 LDCM0438  CL48 HEK-293T C311(1.07)  LDD1642  [12]
 LDCM0439  CL49 HEK-293T C311(0.86)  LDD1643  [12]
 LDCM0445  CL54 HEK-293T C311(1.23)  LDD1648  [12]
 LDCM0448  CL57 HEK-293T C311(1.14)  LDD1651  [12]
 LDCM0449  CL58 HEK-293T C311(1.20)  LDD1652  [12]
 LDCM0450  CL59 HEK-293T C311(1.31)  LDD1653  [12]
 LDCM0451  CL6 HEK-293T C311(1.03)  LDD1654  [12]
 LDCM0452  CL60 HEK-293T C311(1.13)  LDD1655  [12]
 LDCM0453  CL61 HEK-293T C311(0.88)  LDD1656  [12]
 LDCM0455  CL63 HEK-293T C311(1.07)  LDD1658  [12]
 LDCM0458  CL66 HEK-293T C311(1.18)  LDD1661  [12]
 LDCM0461  CL69 HEK-293T C311(1.01)  LDD1664  [12]
 LDCM0463  CL70 HEK-293T C311(1.20)  LDD1666  [12]
 LDCM0464  CL71 HEK-293T C311(1.25)  LDD1667  [12]
 LDCM0465  CL72 HEK-293T C311(1.19)  LDD1668  [12]
 LDCM0466  CL73 HEK-293T C311(0.87)  LDD1669  [12]
 LDCM0471  CL78 HEK-293T C311(1.12)  LDD1674  [12]
 LDCM0475  CL81 HEK-293T C311(1.12)  LDD1678  [12]
 LDCM0476  CL82 HEK-293T C311(1.20)  LDD1679  [12]
 LDCM0477  CL83 HEK-293T C311(1.11)  LDD1680  [12]
 LDCM0478  CL84 HEK-293T C311(1.09)  LDD1681  [12]
 LDCM0479  CL85 HEK-293T C311(0.83)  LDD1682  [12]
 LDCM0481  CL87 HEK-293T C311(0.78)  LDD1684  [12]
 LDCM0484  CL9 HEK-293T C311(1.04)  LDD1687  [12]
 LDCM0485  CL90 HEK-293T C311(1.12)  LDD1688  [12]
 LDCM0488  CL93 HEK-293T C311(1.02)  LDD1691  [12]
 LDCM0489  CL94 HEK-293T C311(1.07)  LDD1692  [12]
 LDCM0490  CL95 HEK-293T C311(1.14)  LDD1693  [12]
 LDCM0491  CL96 HEK-293T C311(0.79)  LDD1694  [12]
 LDCM0492  CL97 HEK-293T C311(1.03)  LDD1695  [12]
 LDCM0494  CL99 HEK-293T C311(0.96)  LDD1697  [12]
 LDCM0495  E2913 HEK-293T C311(1.20)  LDD1698  [12]
 LDCM0625  F8 Ramos C311(0.26)  LDD2187  [13]
 LDCM0572  Fragment10 Ramos C311(1.13)  LDD2189  [13]
 LDCM0573  Fragment11 Ramos C311(0.65)  LDD2190  [13]
 LDCM0574  Fragment12 Ramos C311(0.62)  LDD2191  [13]
 LDCM0575  Fragment13 Ramos C311(5.12)  LDD2192  [13]
 LDCM0576  Fragment14 Ramos C311(1.01)  LDD2193  [13]
 LDCM0579  Fragment20 Ramos C311(0.79)  LDD2194  [13]
 LDCM0580  Fragment21 Ramos C311(10.67)  LDD2195  [13]
 LDCM0582  Fragment23 Ramos C311(0.80)  LDD2196  [13]
 LDCM0578  Fragment27 Ramos C311(8.44)  LDD2197  [13]
 LDCM0586  Fragment28 Ramos C311(0.41)  LDD2198  [13]
 LDCM0589  Fragment31 Ramos C311(5.91)  LDD2200  [13]
 LDCM0590  Fragment32 Ramos C311(0.83)  LDD2201  [13]
 LDCM0468  Fragment33 HEK-293T C311(0.96)  LDD1671  [12]
 LDCM0596  Fragment38 Ramos C311(4.09)  LDD2203  [13]
 LDCM0566  Fragment4 Ramos C311(0.33)  LDD2184  [13]
 LDCM0610  Fragment52 Ramos C311(7.10)  LDD2204  [13]
 LDCM0614  Fragment56 Ramos C311(7.16)  LDD2205  [13]
 LDCM0569  Fragment7 Ramos C311(0.31)  LDD2186  [13]
 LDCM0571  Fragment9 Ramos C311(0.94)  LDD2188  [13]
 LDCM0022  KB02 HEK-293T C311(1.11)  LDD1492  [12]
 LDCM0023  KB03 HEK-293T C311(1.02)  LDD1497  [12]
 LDCM0024  KB05 SNU-1196 C311(1.47)  LDD3439  [4]
 LDCM0109  NEM HeLa N.A.  LDD0225  [6]
 LDCM0501  Nucleophilic fragment 13b MDA-MB-231 C311(0.91)  LDD2094  [5]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Dual specificity protein kinase CLK2 (CLK2) CMGC Ser/Thr protein kinase family P49760
E3 ubiquitin-protein ligase TRIM23 (TRIM23) Arf family P36406
Other
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Lipopolysaccharide-induced tumor necrosis factor-alpha factor (LITAF) CDIP1/LITAF family Q99732

References

1 Quantitative and Site-Specific Chemoproteomic Profiling of Targets of Acrolein. Chem Res Toxicol. 2019 Mar 18;32(3):467-473. doi: 10.1021/acs.chemrestox.8b00343. Epub 2019 Jan 15.
2 Cyclopropenone, Cyclopropeniminium Ion, and Cyclopropenethione as Novel Electrophilic Warheads for Potential Target Discovery of Triple-Negative Breast Cancer. J Med Chem. 2023 Feb 23;66(4):2851-2864. doi: 10.1021/acs.jmedchem.2c01889. Epub 2023 Feb 10.
3 Low-Toxicity Sulfonium-Based Probes for Cysteine-Specific Profiling in Live Cells. Anal Chem. 2022 Mar 15;94(10):4366-4372. doi: 10.1021/acs.analchem.1c05129. Epub 2022 Mar 4.
4 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
5 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
6 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
7 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
8 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
9 Oxidative cyclization reagents reveal tryptophan cation- interactions. Nature. 2024 Mar;627(8004):680-687. doi: 10.1038/s41586-024-07140-6. Epub 2024 Mar 6.
Mass spectrometry data entry: PXD001377 , PXD005252
10 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.
11 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
12 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
13 Site-specific quantitative cysteine profiling with data-independent acquisition-based mass spectrometry. Methods Enzymol. 2023;679:295-322. doi: 10.1016/bs.mie.2022.07.037. Epub 2022 Sep 7.
Mass spectrometry data entry: PXD027578