Details of the Target

General Information of Target

Target ID LDTP14998
Target Name Putative hydroxypyruvate isomerase (HYI)
Gene Name HYI
Gene ID 81888
Synonyms
Putative hydroxypyruvate isomerase; EC 5.3.1.22; Endothelial cell apoptosis protein E-CE1
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MQKIVQTDEITNTQAFRKGKRKRTETMDSENANSDMDKGQRDPYSGNAFLPGESSSEDEE
PLAELSKEELCAKIKSLKEKLTNTRKENSRLRQSLVMLQVLPQAVTQFEELVGMAEALLK
GGGTMSTSASTLWRATNNSSPDSFASTCSNSNSNSSSPVSLKPEEEHQTDEKQFQIEKWQ
IARCNKSKPQKFINDLMQVLYTNEYMATHSLTGAKSSTSRDKAVKPAMNQNEVQEIIGVT
KQLFPNTDDVSIRRMIGQKLNNCTKKPNLSKNLNSQDIK
Target Bioclass
Enzyme
Family
Hyi family
Function Catalyzes the reversible isomerization between hydroxypyruvate and 2-hydroxy-3-oxopropanoate (also termed tartronate semialdehyde).
Uniprot ID
Q5T013
Ensemble ID
ENST00000372430.9
HGNC ID
HGNC:26948

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
JURKAT SNV: p.V146M .
LNCaP clone FGC SNV: p.A137D .
LS123 SNV: p.A171G .
MFE319 SNV: p.I201T .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 3 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
IPM
 Probe Info  		C102(0.56)  LDD2229  [1]
BTD
 Probe Info  		C102(1.45)  LDD1700  [2]
DBIA
 Probe Info  		C248(1.04)  LDD1095  [3]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0524  2-Cyano-N-(2-morpholin-4-yl-ethyl)-acetamide MDA-MB-231 C102(0.71)  LDD2117  [2]
 LDCM0216  AC100 PaTu 8988t C248(1.04)  LDD1095  [3]
 LDCM0217  AC101 PaTu 8988t C248(0.83)  LDD1096  [3]
 LDCM0218  AC102 PaTu 8988t C248(1.75)  LDD1097  [3]
 LDCM0219  AC103 PaTu 8988t C248(2.04)  LDD1098  [3]
 LDCM0220  AC104 PaTu 8988t C248(0.90)  LDD1099  [3]
 LDCM0221  AC105 PaTu 8988t C248(0.95)  LDD1100  [3]
 LDCM0222  AC106 PaTu 8988t C248(1.44)  LDD1101  [3]
 LDCM0223  AC107 PaTu 8988t C248(1.13)  LDD1102  [3]
 LDCM0224  AC108 PaTu 8988t C248(1.04)  LDD1103  [3]
 LDCM0225  AC109 PaTu 8988t C248(1.17)  LDD1104  [3]
 LDCM0227  AC110 PaTu 8988t C248(1.68)  LDD1106  [3]
 LDCM0228  AC111 PaTu 8988t C248(2.07)  LDD1107  [3]
 LDCM0229  AC112 PaTu 8988t C248(1.53)  LDD1108  [3]
 LDCM0230  AC113 PaTu 8988t C248(0.98)  LDD1109  [3]
 LDCM0231  AC114 PaTu 8988t C248(1.04)  LDD1110  [3]
 LDCM0232  AC115 PaTu 8988t C248(0.89)  LDD1111  [3]
 LDCM0233  AC116 PaTu 8988t C248(1.11)  LDD1112  [3]
 LDCM0234  AC117 PaTu 8988t C248(1.16)  LDD1113  [3]
 LDCM0235  AC118 PaTu 8988t C248(0.73)  LDD1114  [3]
 LDCM0236  AC119 PaTu 8988t C248(1.26)  LDD1115  [3]
 LDCM0238  AC120 PaTu 8988t C248(0.76)  LDD1117  [3]
 LDCM0239  AC121 PaTu 8988t C248(1.29)  LDD1118  [3]
 LDCM0240  AC122 PaTu 8988t C248(0.71)  LDD1119  [3]
 LDCM0241  AC123 PaTu 8988t C248(0.73)  LDD1120  [3]
 LDCM0242  AC124 PaTu 8988t C248(0.87)  LDD1121  [3]
 LDCM0243  AC125 PaTu 8988t C248(0.70)  LDD1122  [3]
 LDCM0244  AC126 PaTu 8988t C248(0.90)  LDD1123  [3]
 LDCM0245  AC127 PaTu 8988t C248(0.77)  LDD1124  [3]
 LDCM0296  AC35 PaTu 8988t C248(1.04)  LDD1175  [3]
 LDCM0297  AC36 PaTu 8988t C248(1.22)  LDD1176  [3]
 LDCM0298  AC37 PaTu 8988t C248(1.14)  LDD1177  [3]
 LDCM0299  AC38 PaTu 8988t C248(1.33)  LDD1178  [3]
 LDCM0300  AC39 PaTu 8988t C248(1.36)  LDD1179  [3]
 LDCM0302  AC40 PaTu 8988t C248(1.23)  LDD1181  [3]
 LDCM0303  AC41 PaTu 8988t C248(1.16)  LDD1182  [3]
 LDCM0304  AC42 PaTu 8988t C248(1.38)  LDD1183  [3]
 LDCM0305  AC43 PaTu 8988t C248(1.24)  LDD1184  [3]
 LDCM0306  AC44 PaTu 8988t C248(1.13)  LDD1185  [3]
 LDCM0307  AC45 PaTu 8988t C248(1.06)  LDD1186  [3]
 LDCM0365  AC98 PaTu 8988t C248(1.42)  LDD1244  [3]
 LDCM0366  AC99 PaTu 8988t C248(1.23)  LDD1245  [3]
 LDCM0387  CL117 PaTu 8988t C248(1.19)  LDD1266  [3]
 LDCM0388  CL118 PaTu 8988t C248(1.10)  LDD1267  [3]
 LDCM0389  CL119 PaTu 8988t C248(1.20)  LDD1268  [3]
 LDCM0391  CL120 PaTu 8988t C248(1.09)  LDD1270  [3]
 LDCM0420  CL31 PaTu 8988t C248(0.71)  LDD1299  [3]
 LDCM0421  CL32 PaTu 8988t C248(0.97)  LDD1300  [3]
 LDCM0422  CL33 PaTu 8988t C248(3.27)  LDD1301  [3]
 LDCM0423  CL34 PaTu 8988t C248(1.39)  LDD1302  [3]
 LDCM0424  CL35 PaTu 8988t C248(1.11)  LDD1303  [3]
 LDCM0425  CL36 PaTu 8988t C248(1.13)  LDD1304  [3]
 LDCM0426  CL37 PaTu 8988t C248(1.33)  LDD1305  [3]
 LDCM0428  CL39 PaTu 8988t C248(1.24)  LDD1307  [3]
 LDCM0430  CL40 PaTu 8988t C248(1.02)  LDD1309  [3]
 LDCM0431  CL41 PaTu 8988t C248(2.98)  LDD1310  [3]
 LDCM0432  CL42 PaTu 8988t C248(1.35)  LDD1311  [3]
 LDCM0433  CL43 PaTu 8988t C248(1.59)  LDD1312  [3]
 LDCM0434  CL44 PaTu 8988t C248(1.37)  LDD1313  [3]
 LDCM0435  CL45 PaTu 8988t C248(0.89)  LDD1314  [3]
 LDCM0436  CL46 PaTu 8988t C248(1.51)  LDD1315  [3]
 LDCM0437  CL47 PaTu 8988t C248(1.21)  LDD1316  [3]
 LDCM0438  CL48 PaTu 8988t C248(1.38)  LDD1317  [3]
 LDCM0439  CL49 PaTu 8988t C248(1.50)  LDD1318  [3]
 LDCM0441  CL50 PaTu 8988t C248(1.88)  LDD1320  [3]
 LDCM0442  CL51 PaTu 8988t C248(3.04)  LDD1321  [3]
 LDCM0443  CL52 PaTu 8988t C248(1.94)  LDD1322  [3]
 LDCM0444  CL53 PaTu 8988t C248(1.69)  LDD1323  [3]
 LDCM0445  CL54 PaTu 8988t C248(2.79)  LDD1324  [3]
 LDCM0446  CL55 PaTu 8988t C248(1.81)  LDD1325  [3]
 LDCM0447  CL56 PaTu 8988t C248(0.45)  LDD1326  [3]
 LDCM0448  CL57 PaTu 8988t C248(2.28)  LDD1327  [3]
 LDCM0449  CL58 PaTu 8988t C248(2.39)  LDD1328  [3]
 LDCM0450  CL59 PaTu 8988t C248(2.12)  LDD1329  [3]
 LDCM0452  CL60 PaTu 8988t C248(1.99)  LDD1331  [3]
 LDCM0427  Fragment51 PaTu 8988t C248(0.78)  LDD1306  [3]
 LDCM0497  Nucleophilic fragment 11b MDA-MB-231 C102(0.97)  LDD2090  [2]
 LDCM0500  Nucleophilic fragment 13a MDA-MB-231 C102(1.49)  LDD2093  [2]
 LDCM0506  Nucleophilic fragment 16a MDA-MB-231 C102(0.81)  LDD2099  [2]
 LDCM0508  Nucleophilic fragment 17a MDA-MB-231 C102(4.60)  LDD2101  [2]
 LDCM0512  Nucleophilic fragment 19a MDA-MB-231 C102(1.05)  LDD2105  [2]
 LDCM0514  Nucleophilic fragment 20a MDA-MB-231 C102(0.95)  LDD2107  [2]
 LDCM0516  Nucleophilic fragment 21a MDA-MB-231 C102(0.67)  LDD2109  [2]
 LDCM0518  Nucleophilic fragment 22a MDA-MB-231 C102(1.05)  LDD2111  [2]
 LDCM0525  Nucleophilic fragment 25b MDA-MB-231 C102(0.69)  LDD2118  [2]
 LDCM0526  Nucleophilic fragment 26a MDA-MB-231 C102(1.63)  LDD2119  [2]
 LDCM0530  Nucleophilic fragment 28a MDA-MB-231 C102(1.04)  LDD2123  [2]
 LDCM0532  Nucleophilic fragment 29a MDA-MB-231 C102(1.02)  LDD2125  [2]
 LDCM0534  Nucleophilic fragment 30a MDA-MB-231 C102(0.96)  LDD2127  [2]
 LDCM0536  Nucleophilic fragment 31 MDA-MB-231 C102(1.03)  LDD2129  [2]
 LDCM0540  Nucleophilic fragment 35 MDA-MB-231 C102(1.47)  LDD2133  [2]
 LDCM0541  Nucleophilic fragment 36 MDA-MB-231 C102(1.52)  LDD2134  [2]
 LDCM0543  Nucleophilic fragment 38 MDA-MB-231 C102(0.90)  LDD2136  [2]
 LDCM0544  Nucleophilic fragment 39 MDA-MB-231 C102(1.19)  LDD2137  [2]
 LDCM0211  Nucleophilic fragment 3b MDA-MB-231 C102(1.45)  LDD1700  [2]
 LDCM0546  Nucleophilic fragment 40 MDA-MB-231 C102(0.81)  LDD2140  [2]
 LDCM0550  Nucleophilic fragment 5a MDA-MB-231 C102(2.17)  LDD2144  [2]
 LDCM0552  Nucleophilic fragment 6a MDA-MB-231 C102(0.88)  LDD2146  [2]
 LDCM0554  Nucleophilic fragment 7a MDA-MB-231 C102(1.51)  LDD2148  [2]
 LDCM0556  Nucleophilic fragment 8a MDA-MB-231 C102(0.64)  LDD2150  [2]
 LDCM0559  Nucleophilic fragment 9b MDA-MB-231 C102(1.45)  LDD2153  [2]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Other
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Serine/arginine-rich splicing factor 11 (SRSF11) Splicing factor SR family Q05519
Testis-expressed protein 11 (TEX11) SPO22 family Q8IYF3

References

1 A quantitative thiol reactivity profiling platform to analyze redox and electrophile reactive cysteine proteomes. Nat Protoc. 2020 Sep;15(9):2891-2919. doi: 10.1038/s41596-020-0352-2. Epub 2020 Jul 20.
Mass spectrometry data entry: PXD016048
2 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
3 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.