Details of the Target

General Information of Target

Target ID LDTP14305
Target Name Bis(5'-adenosyl)-triphosphatase ENPP4 (ENPP4)
Gene Name ENPP4
Gene ID 22875
Synonyms
KIAA0879; NPP4; Bis(5'-adenosyl)-triphosphatase ENPP4; EC 3.6.1.29; AP3A hydrolase; AP3Aase; Ectonucleotide pyrophosphatase/phosphodiesterase family member 4; E-NPP 4; NPP-4
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MAAQAAAAAQAAAAQAAQAEAADSWYLALLGFAEHFRTSSPPKIRLCVHCLQAVFPFKPP
QRIEARTHLQLGSVLYHHTKNSEQARSHLEKAWLISQQIPQFEDVKFEAASLLSELYCQE
NSVDAAKPLLRKAIQISQQTPYWHCRLLFQLAQLHTLEKDLVSACDLLGVGAEYARVVGS
EYTRALFLLSKGMLLLMERKLQEVHPLLTLCGQIVENWQGNPIQKESLRVFFLVLQVTHY
LDAGQVKSVKPCLKQLQQCIQTISTLHDDEILPSNPADLFHWLPKEHMCVLVYLVTVMHS
MQAGYLEKAQKYTDKALMQLEKLKMLDCSPILSSFQVILLEHIIMCRLVTGHKATALQEI
SQVCQLCQQSPRLFSNHAAQLHTLLGLYCVSVNCMDNAEAQFTTALRLTNHQELWAFIVT
NLASVYIREGNRHQEVLYSLLERINPDHSFPVSSHCLRAAAFYVRGLFSFFQGRYNEAKR
FLRETLKMSNAEDLNRLTACSLVLLGHIFYVLGNHRESNNMVVPAMQLASKIPDMSVQLW
SSALLRDLNKACGNAMDAHEAAQMHQNFSQQLLQDHIEACSLPEHNLITWTDGPPPVQFQ
AQNGPNTSLASLL
Target Bioclass
Enzyme
Family
Nucleotide pyrophosphatase/phosphodiesterase family
Subcellular location
Cell membrane
Function
Hydrolyzes extracellular Ap3A into AMP and ADP, and Ap4A into AMP and ATP. Ap3A and Ap4A are diadenosine polyphosphates thought to induce proliferation of vascular smooth muscle cells. Acts as a procoagulant, mediating platelet aggregation at the site of nascent thrombus via release of ADP from Ap3A and activation of ADP receptors.
Uniprot ID
Q9Y6X5
Ensemble ID
ENST00000321037.5
HGNC ID
HGNC:3359

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 4 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
STPyne
 Probe Info  		K283(10.00)  LDD0277  [1]
4-Iodoacetamidophenylacetylene
 Probe Info  		C254(0.00); C287(0.00)  LDD0038  [2]
IA-alkyne
 Probe Info  		C254(0.00); C287(0.00)  LDD0036  [2]
Lodoacetamide azide
 Probe Info  		C287(0.00); C254(0.00)  LDD0037  [2]
PAL-AfBPP Probe
Click To Hide/Show 4 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C112
 Probe Info  		28.64  LDD1799  [3]
C115
 Probe Info  		6.59  LDD1802  [3]
C170
 Probe Info  		20.82  LDD1850  [3]
C171
 Probe Info  		6.32  LDD1851  [3]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Transporter and channel
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Bax inhibitor 1 (TMBIM6) BI1 family P55061
BOS complex subunit TMEM147 (TMEM147) TMEM147 family Q9BVK8

References

1 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
2 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
3 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587