Details of the Target

General Information of Target

Target ID LDTP13708
Target Name E3 ubiquitin-protein ligase PDZRN3 (PDZRN3)
Gene Name PDZRN3
Gene ID 23024
Synonyms
KIAA1095; LNX3; SEMCAP3; E3 ubiquitin-protein ligase PDZRN3; EC 2.3.2.27; Ligand of Numb protein X 3; PDZ domain-containing RING finger protein 3; RING-type E3 ubiquitin transferase PDZRN3; Semaphorin cytoplasmic domain-associated protein 3; Protein SEMACAP3
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MERSPDVSPGPSRSFKEELLCAVCYDPFRDAVTLRCGHNFCRGCVSRCWEVQVSPTCPVC
KDRASPADLRTNHTLNNLVEKLLREEAEGARWTSYRFSRVCRLHRGQLSLFCLEDKELLC
CSCQADPRHQGHRVQPVKDTAHDFRAKCRNMEHALREKAKAFWAMRRSYEAIAKHNQVEA
AWLEGRIRQEFDKLREFLRVEEQAILDAMAEETRQKQLLADEKMKQLTEETEVLAHEIER
LQMEMKEDDVSFLMKHKSRKRRLFCTMEPEPVQPGMLIDVCKYLGSLQYRVWKKMLASVE
SVPFSFDPNTAAGWLSVSDDLTSVTNHGYRVQVENPERFSSAPCLLGSRVFSQGSHAWEV
ALGGLQSWRVGVVRVRQDSGAEGHSHSCYHDTRSGFWYVCRTQGVEGDHCVTSDPATSPL
VLAIPRRLRVELECEEGELSFYDAERHCHLYTFHARFGEVRPYFYLGGARGAGPPEPLRI
CPLHISVKEELDG
Target Bioclass
Enzyme
Subcellular location
Synapse
Function
E3 ubiquitin-protein ligase. Plays an important role in regulating the surface level of MUSK on myotubes. Mediates the ubiquitination of MUSK, promoting its endocytosis and lysosomal degradation. Might contribute to terminal myogenic differentiation.
Uniprot ID
Q9UPQ7
Ensemble ID
ENST00000263666.9
HGNC ID
HGNC:17704

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
CAL33 SNV: p.G674C .
COLO792 SNV: p.P678S DBIA    Probe Info 
EFO27 SNV: p.R693H .
HCT15 SNV: p.D753N .
HUH1 SNV: p.C436R .
IGROV1 SNV: p.H101P; p.R971C; p.Q988R DBIA    Probe Info 
KMCH1 SNV: p.P639S .
NCIH1944 Deletion: p.S247VfsTer2 .
NCIH1993 SNV: p.H67L .
NCIN87 SNV: p.E195K .
PATU8988T SNV: p.R931C .
REH SNV: p.L809H .
SNU1079 SNV: p.R314I .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 5 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
IPM
 Probe Info  		C161(1.85)  LDD2229  [1]
AHL-Pu-1
 Probe Info  		C764(4.67); C161(2.54)  LDD0170  [2]
HHS-475
 Probe Info  		Y666(1.49)  LDD0264  [3]
IA-alkyne
 Probe Info  		C134(5.37)  LDD0315  [4]
DBIA
 Probe Info  		C142(1.58); C146(1.58)  LDD0080  [5]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0025  4SU-RNA DM93 C764(4.67); C161(2.54)  LDD0170  [2]
 LDCM0026  4SU-RNA+native RNA DM93 C764(4.32)  LDD0171  [2]
 LDCM0214  AC1 HEK-293T C162(1.01)  LDD1507  [6]
 LDCM0215  AC10 HEK-293T C162(1.11)  LDD1508  [6]
 LDCM0226  AC11 HEK-293T C162(1.01)  LDD1509  [6]
 LDCM0237  AC12 HEK-293T C117(1.13); C161(0.99)  LDD1510  [6]
 LDCM0259  AC14 HEK-293T C162(0.90)  LDD1512  [6]
 LDCM0276  AC17 HEK-293T C162(1.09)  LDD1515  [6]
 LDCM0277  AC18 HEK-293T C162(0.96)  LDD1516  [6]
 LDCM0278  AC19 HEK-293T C162(0.27)  LDD1517  [6]
 LDCM0279  AC2 HEK-293T C162(0.90)  LDD1518  [6]
 LDCM0280  AC20 HEK-293T C117(0.92); C161(0.91)  LDD1519  [6]
 LDCM0281  AC21 HEK-293T C117(0.94); C233(1.03); C162(0.89)  LDD1520  [6]
 LDCM0282  AC22 HEK-293T C162(0.94)  LDD1521  [6]
 LDCM0284  AC24 HEK-293T C117(1.06); C162(1.04)  LDD1523  [6]
 LDCM0285  AC25 HEK-293T C162(1.26)  LDD1524  [6]
 LDCM0286  AC26 HEK-293T C162(0.98)  LDD1525  [6]
 LDCM0287  AC27 HEK-293T C162(0.96)  LDD1526  [6]
 LDCM0288  AC28 HEK-293T C117(0.98); C161(1.08)  LDD1527  [6]
 LDCM0289  AC29 HEK-293T C117(1.03); C233(0.97); C162(0.95)  LDD1528  [6]
 LDCM0290  AC3 HEK-293T C162(1.16)  LDD1529  [6]
 LDCM0291  AC30 HEK-293T C162(1.10)  LDD1530  [6]
 LDCM0293  AC32 HEK-293T C117(0.98); C162(1.30)  LDD1532  [6]
 LDCM0294  AC33 HEK-293T C162(0.83)  LDD1533  [6]
 LDCM0295  AC34 HEK-293T C162(1.06)  LDD1534  [6]
 LDCM0296  AC35 HEK-293T C162(0.89)  LDD1535  [6]
 LDCM0297  AC36 HEK-293T C117(1.11); C161(1.01)  LDD1536  [6]
 LDCM0298  AC37 HEK-293T C117(0.99); C233(0.95); C162(1.07)  LDD1537  [6]
 LDCM0299  AC38 HEK-293T C162(1.05)  LDD1538  [6]
 LDCM0301  AC4 HEK-293T C117(0.93); C161(1.00)  LDD1540  [6]
 LDCM0302  AC40 HEK-293T C117(1.00); C162(1.07)  LDD1541  [6]
 LDCM0303  AC41 HEK-293T C162(0.86)  LDD1542  [6]
 LDCM0304  AC42 HEK-293T C162(1.03)  LDD1543  [6]
 LDCM0305  AC43 HEK-293T C162(0.91)  LDD1544  [6]
 LDCM0306  AC44 HEK-293T C117(1.02); C161(1.07)  LDD1545  [6]
 LDCM0307  AC45 HEK-293T C117(1.00); C233(1.02); C162(0.92)  LDD1546  [6]
 LDCM0308  AC46 HEK-293T C162(1.31)  LDD1547  [6]
 LDCM0310  AC48 HEK-293T C117(1.09); C162(0.97)  LDD1549  [6]
 LDCM0311  AC49 HEK-293T C162(0.99)  LDD1550  [6]
 LDCM0312  AC5 HEK-293T C117(1.00); C233(0.95); C162(0.75)  LDD1551  [6]
 LDCM0313  AC50 HEK-293T C162(1.19)  LDD1552  [6]
 LDCM0314  AC51 HEK-293T C162(1.09)  LDD1553  [6]
 LDCM0315  AC52 HEK-293T C117(0.96); C161(1.04)  LDD1554  [6]
 LDCM0316  AC53 HEK-293T C117(0.93); C233(1.05); C162(1.01)  LDD1555  [6]
 LDCM0317  AC54 HEK-293T C162(0.91)  LDD1556  [6]
 LDCM0319  AC56 HEK-293T C117(0.91); C162(1.64)  LDD1558  [6]
 LDCM0320  AC57 HEK-293T C162(1.13)  LDD1559  [6]
 LDCM0321  AC58 HEK-293T C162(0.95)  LDD1560  [6]
 LDCM0322  AC59 HEK-293T C162(0.96)  LDD1561  [6]
 LDCM0323  AC6 HEK-293T C162(0.91)  LDD1562  [6]
 LDCM0324  AC60 HEK-293T C117(1.05); C161(0.96)  LDD1563  [6]
 LDCM0325  AC61 HEK-293T C117(0.94); C233(1.13); C162(1.04)  LDD1564  [6]
 LDCM0326  AC62 HEK-293T C162(0.82)  LDD1565  [6]
 LDCM0328  AC64 HEK-293T C117(1.05); C162(1.22)  LDD1567  [6]
 LDCM0345  AC8 HEK-293T C117(1.01); C162(1.66)  LDD1569  [6]
 LDCM0248  AKOS034007472 HEK-293T C117(1.02); C233(0.98); C162(0.93)  LDD1511  [6]
 LDCM0356  AKOS034007680 HEK-293T C162(1.03)  LDD1570  [6]
 LDCM0275  AKOS034007705 HEK-293T C117(1.14); C162(1.12)  LDD1514  [6]
 LDCM0367  CL1 HEK-293T C117(0.87); C162(1.21)  LDD1571  [6]
 LDCM0368  CL10 HEK-293T C162(1.67)  LDD1572  [6]
 LDCM0369  CL100 HEK-293T C117(1.05)  LDD1573  [6]
 LDCM0370  CL101 HEK-293T C117(0.99); C162(1.05)  LDD1574  [6]
 LDCM0371  CL102 HEK-293T C117(1.04); C162(1.35)  LDD1575  [6]
 LDCM0372  CL103 HEK-293T C117(1.06)  LDD1576  [6]
 LDCM0373  CL104 HEK-293T C117(1.01)  LDD1577  [6]
 LDCM0374  CL105 HEK-293T C117(1.02); C162(1.00)  LDD1578  [6]
 LDCM0375  CL106 HEK-293T C117(0.96); C162(1.19)  LDD1579  [6]
 LDCM0376  CL107 HEK-293T C117(0.98)  LDD1580  [6]
 LDCM0377  CL108 HEK-293T C117(1.10)  LDD1581  [6]
 LDCM0378  CL109 HEK-293T C117(0.91); C162(1.07)  LDD1582  [6]
 LDCM0380  CL110 HEK-293T C117(1.00); C162(1.01)  LDD1584  [6]
 LDCM0381  CL111 HEK-293T C117(0.92)  LDD1585  [6]
 LDCM0382  CL112 HEK-293T C117(1.25)  LDD1586  [6]
 LDCM0383  CL113 HEK-293T C117(1.02); C162(0.87)  LDD1587  [6]
 LDCM0384  CL114 HEK-293T C117(1.05); C162(1.06)  LDD1588  [6]
 LDCM0385  CL115 HEK-293T C117(0.97)  LDD1589  [6]
 LDCM0386  CL116 HEK-293T C117(1.09)  LDD1590  [6]
 LDCM0387  CL117 HEK-293T C117(0.88); C162(0.92)  LDD1591  [6]
 LDCM0388  CL118 HEK-293T C117(1.06); C162(0.89)  LDD1592  [6]
 LDCM0389  CL119 HEK-293T C117(1.05)  LDD1593  [6]
 LDCM0390  CL12 HEK-293T C117(1.03); C162(1.23)  LDD1594  [6]
 LDCM0391  CL120 HEK-293T C117(1.08)  LDD1595  [6]
 LDCM0392  CL121 HEK-293T C117(0.87); C162(0.87)  LDD1596  [6]
 LDCM0393  CL122 HEK-293T C117(1.02); C162(0.95)  LDD1597  [6]
 LDCM0394  CL123 HEK-293T C117(1.05)  LDD1598  [6]
 LDCM0395  CL124 HEK-293T C117(1.04)  LDD1599  [6]
 LDCM0396  CL125 HEK-293T C117(0.96); C162(0.82)  LDD1600  [6]
 LDCM0397  CL126 HEK-293T C117(1.02); C162(1.12)  LDD1601  [6]
 LDCM0398  CL127 HEK-293T C117(0.90)  LDD1602  [6]
 LDCM0399  CL128 HEK-293T C117(1.06)  LDD1603  [6]
 LDCM0400  CL13 HEK-293T C117(0.89); C162(0.56)  LDD1604  [6]
 LDCM0401  CL14 HEK-293T C117(0.99); C162(1.20)  LDD1605  [6]
 LDCM0402  CL15 HEK-293T C117(1.00)  LDD1606  [6]
 LDCM0403  CL16 HEK-293T C117(1.06)  LDD1607  [6]
 LDCM0404  CL17 HEK-293T C162(1.38)  LDD1608  [6]
 LDCM0405  CL18 HEK-293T C162(1.15)  LDD1609  [6]
 LDCM0406  CL19 HEK-293T C162(1.16)  LDD1610  [6]
 LDCM0407  CL2 HEK-293T C117(0.93); C162(1.05)  LDD1611  [6]
 LDCM0408  CL20 HEK-293T C117(1.03); C161(0.90)  LDD1612  [6]
 LDCM0409  CL21 HEK-293T C117(1.05); C233(1.14); C162(1.72)  LDD1613  [6]
 LDCM0410  CL22 HEK-293T C162(1.01)  LDD1614  [6]
 LDCM0412  CL24 HEK-293T C117(1.07); C162(1.27)  LDD1616  [6]
 LDCM0413  CL25 HEK-293T C117(0.86); C162(0.85)  LDD1617  [6]
 LDCM0414  CL26 HEK-293T C117(1.10); C162(1.36)  LDD1618  [6]
 LDCM0415  CL27 HEK-293T C117(0.96)  LDD1619  [6]
 LDCM0416  CL28 HEK-293T C117(0.97)  LDD1620  [6]
 LDCM0417  CL29 HEK-293T C162(1.11)  LDD1621  [6]
 LDCM0418  CL3 HEK-293T C117(1.08)  LDD1622  [6]
 LDCM0419  CL30 HEK-293T C162(1.04)  LDD1623  [6]
 LDCM0420  CL31 HEK-293T C162(1.05)  LDD1624  [6]
 LDCM0421  CL32 HEK-293T C117(1.02); C161(0.98)  LDD1625  [6]
 LDCM0422  CL33 HEK-293T C117(1.03); C233(1.06); C162(1.28)  LDD1626  [6]
 LDCM0423  CL34 HEK-293T C162(1.12)  LDD1627  [6]
 LDCM0425  CL36 HEK-293T C117(1.23); C162(1.10)  LDD1629  [6]
 LDCM0426  CL37 HEK-293T C117(0.98); C162(1.14)  LDD1630  [6]
 LDCM0428  CL39 HEK-293T C117(0.99)  LDD1632  [6]
 LDCM0429  CL4 HEK-293T C117(1.08)  LDD1633  [6]
 LDCM0430  CL40 HEK-293T C117(1.01)  LDD1634  [6]
 LDCM0431  CL41 HEK-293T C162(1.05)  LDD1635  [6]
 LDCM0432  CL42 HEK-293T C162(0.96)  LDD1636  [6]
 LDCM0433  CL43 HEK-293T C162(0.91)  LDD1637  [6]
 LDCM0434  CL44 HEK-293T C117(1.05); C161(0.96)  LDD1638  [6]
 LDCM0435  CL45 HEK-293T C117(0.94); C233(1.06); C162(1.14)  LDD1639  [6]
 LDCM0436  CL46 HEK-293T C162(1.20)  LDD1640  [6]
 LDCM0438  CL48 HEK-293T C117(1.24); C162(0.99)  LDD1642  [6]
 LDCM0439  CL49 HEK-293T C117(0.92); C162(0.95)  LDD1643  [6]
 LDCM0440  CL5 HEK-293T C162(1.06)  LDD1644  [6]
 LDCM0441  CL50 HEK-293T C117(0.96); C162(1.22)  LDD1645  [6]
 LDCM0443  CL52 HEK-293T C117(1.03)  LDD1646  [6]
 LDCM0444  CL53 HEK-293T C162(0.94)  LDD1647  [6]
 LDCM0445  CL54 HEK-293T C162(1.25)  LDD1648  [6]
 LDCM0446  CL55 HEK-293T C162(1.08)  LDD1649  [6]
 LDCM0447  CL56 HEK-293T C117(1.00); C161(1.39)  LDD1650  [6]
 LDCM0448  CL57 HEK-293T C117(1.11); C233(1.10); C162(1.00)  LDD1651  [6]
 LDCM0449  CL58 HEK-293T C162(1.29)  LDD1652  [6]
 LDCM0451  CL6 HEK-293T C162(1.27)  LDD1654  [6]
 LDCM0452  CL60 HEK-293T C117(1.05); C162(1.28)  LDD1655  [6]
 LDCM0453  CL61 HEK-293T C117(1.14); C162(0.87)  LDD1656  [6]
 LDCM0454  CL62 HEK-293T C117(1.02); C162(0.99)  LDD1657  [6]
 LDCM0455  CL63 HEK-293T C117(1.14)  LDD1658  [6]
 LDCM0456  CL64 HEK-293T C117(1.05)  LDD1659  [6]
 LDCM0457  CL65 HEK-293T C162(0.88)  LDD1660  [6]
 LDCM0458  CL66 HEK-293T C162(1.52)  LDD1661  [6]
 LDCM0459  CL67 HEK-293T C162(1.03)  LDD1662  [6]
 LDCM0460  CL68 HEK-293T C117(0.94); C161(1.35)  LDD1663  [6]
 LDCM0461  CL69 HEK-293T C117(1.15); C233(1.01); C162(1.28)  LDD1664  [6]
 LDCM0462  CL7 HEK-293T C162(0.92)  LDD1665  [6]
 LDCM0463  CL70 HEK-293T C162(0.82)  LDD1666  [6]
 LDCM0465  CL72 HEK-293T C117(0.99); C162(0.96)  LDD1668  [6]
 LDCM0466  CL73 HEK-293T C117(1.05); C162(0.79)  LDD1669  [6]
 LDCM0467  CL74 HEK-293T C117(1.10); C162(0.98)  LDD1670  [6]
 LDCM0469  CL76 HEK-293T C117(1.05)  LDD1672  [6]
 LDCM0470  CL77 HEK-293T C162(1.56)  LDD1673  [6]
 LDCM0471  CL78 HEK-293T C162(1.04)  LDD1674  [6]
 LDCM0472  CL79 HEK-293T C162(0.99)  LDD1675  [6]
 LDCM0473  CL8 HEK-293T C117(0.87); C161(1.90)  LDD1676  [6]
 LDCM0474  CL80 HEK-293T C117(0.98); C161(0.96)  LDD1677  [6]
 LDCM0475  CL81 HEK-293T C117(1.06); C233(1.00); C162(1.15)  LDD1678  [6]
 LDCM0476  CL82 HEK-293T C162(1.05)  LDD1679  [6]
 LDCM0478  CL84 HEK-293T C117(0.97); C162(0.98)  LDD1681  [6]
 LDCM0479  CL85 HEK-293T C117(0.92); C162(0.86)  LDD1682  [6]
 LDCM0480  CL86 HEK-293T C117(0.96); C162(1.18)  LDD1683  [6]
 LDCM0481  CL87 HEK-293T C117(1.05)  LDD1684  [6]
 LDCM0482  CL88 HEK-293T C117(1.14)  LDD1685  [6]
 LDCM0483  CL89 HEK-293T C162(1.46)  LDD1686  [6]
 LDCM0484  CL9 HEK-293T C117(1.23); C233(1.03); C162(1.12)  LDD1687  [6]
 LDCM0485  CL90 HEK-293T C162(1.99)  LDD1688  [6]
 LDCM0486  CL91 HEK-293T C162(0.84)  LDD1689  [6]
 LDCM0487  CL92 HEK-293T C117(0.99); C161(1.97)  LDD1690  [6]
 LDCM0488  CL93 HEK-293T C117(0.98); C233(1.07); C162(1.02)  LDD1691  [6]
 LDCM0489  CL94 HEK-293T C162(1.02)  LDD1692  [6]
 LDCM0491  CL96 HEK-293T C117(0.86); C162(1.50)  LDD1694  [6]
 LDCM0492  CL97 HEK-293T C117(0.84); C162(1.12)  LDD1695  [6]
 LDCM0493  CL98 HEK-293T C117(1.02); C162(1.21)  LDD1696  [6]
 LDCM0494  CL99 HEK-293T C117(0.97)  LDD1697  [6]
 LDCM0495  E2913 HEK-293T C117(1.02)  LDD1698  [6]
 LDCM0468  Fragment33 HEK-293T C117(1.00)  LDD1671  [6]
 LDCM0427  Fragment51 HEK-293T C117(1.03); C162(1.06)  LDD1631  [6]
 LDCM0116  HHS-0101 DM93 Y666(1.49)  LDD0264  [3]
 LDCM0117  HHS-0201 DM93 Y666(0.37)  LDD0265  [3]
 LDCM0119  HHS-0401 DM93 Y666(1.66)  LDD0267  [3]
 LDCM0022  KB02 HCT 116 C142(1.58); C146(1.58)  LDD0080  [5]
 LDCM0023  KB03 HCT 116 C142(1.76); C146(1.76)  LDD0081  [5]
 LDCM0024  KB05 HCT 116 C142(1.57); C146(1.57)  LDD0082  [5]

References

1 A quantitative thiol reactivity profiling platform to analyze redox and electrophile reactive cysteine proteomes. Nat Protoc. 2020 Sep;15(9):2891-2919. doi: 10.1038/s41596-020-0352-2. Epub 2020 Jul 20.
Mass spectrometry data entry: PXD016048
2 Chemoproteomic capture of RNA binding activity in living cells. Nat Commun. 2023 Oct 7;14(1):6282. doi: 10.1038/s41467-023-41844-z.
Mass spectrometry data entry: PXD044625
3 Discovery of a Cell-Active SuTEx Ligand of Prostaglandin Reductase 2. Chembiochem. 2021 Jun 15;22(12):2134-2139. doi: 10.1002/cbic.202000879. Epub 2021 Apr 29.
4 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
5 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
6 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402