Details of the Target

General Information of Target

Target ID LDTP13570
Target Name Pre-mRNA-splicing factor ISY1 homolog (ISY1)
Gene Name ISY1
Gene ID 100534599
Synonyms
KIAA1160; Pre-mRNA-splicing factor ISY1 homolog
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MAVSLDDDVPLILTLDEGGSAPLAPSNGLGQEELPSKNGGSYAIHDSQAPSLSSGGESSP
SSPAHNWEMNYQEAAIYLQEGENNDKFFTHPKDAKALAAYLFAHNHLFYLMELATALLLL
LLSLCEAPAVPALRLGIYVHATLELFALMVVVFELCMKLRWLGLHTFIRHKRTMVKTSVL
VVQFVEAIVVLVRQMSHVRVTRALRCIFLVDCRYCGGVRRNLRQIFQSLPPFMDILLLLL
FFMIIFAILGFYLFSPNPSDPYFSTLENSIVSLFVLLTTANFPDVMMPSYSRNPWSCVFF
IVYLSIELYFIMNLLLAVVFDTFNDIEKRKFKSLLLHKRTAIQHAYRLLISQRRPAGISY
RQFEGLMRFYKPRMSARERYLTFKALNQNNTPLLSLKDFYDIYEVAALKWKAKKNREHWF
DELPRTALLIFKGINILVKSKAFQYFMYLVVAVNGVWILVETFMLKGGNFFSKHVPWSYL
VFLTIYGVELFLKVAGLGPVEYLSSGWNLFDFSVTVFAFLGLLALALNMEPFYFIVVLRP
LQLLRLFKLKERYRNVLDTMFELLPRMASLGLTLLIFYYSFAIVGMEFFCGIVFPNCCNT
STVADAYRWRNHTVGNRTVVEEGYYYLNNFDNILNSFVTLFELTVVNNWYIIMEGVTSQT
SHWSRLYFMTFYIVTMVVMTIIVAFILEAFVFRMNYSRKNQDSEVDGGITLEKEISKEEL
VAVLELYREARGASSDVTRLLETLSQMERYQQHSMVFLGRRSRTKSDLSLKMYQEEIQEW
YEEHAREQEQQRQLSSSAAPAAQQPPGSRQRSQTVT
Target Bioclass
Other
Family
ISY1 family
Subcellular location
Nucleus
Function
Component of the spliceosome C complex required for the selective processing of microRNAs during embryonic stem cell differentiation. Required for the biogenesis of all miRNAs from the pri-miR-17-92 primary transcript except miR-92a. Only required for the biogenesis of miR-290 and miR-96 from the pri-miR-290-295 and pri-miR-96-183 primary transcripts, respectively. Required during the transition of embryonic stem cells (ESCs) from the naive to primed state. By enhancing miRNA biogenesis, promotes exit of ESCs from the naive state to an intermediate state of poised pluripotency, which precedes transition to the primed state. Involved in pre-mRNA splicing as component of the spliceosome.
Uniprot ID
Q9ULR0
Ensemble ID
ENST00000273541.12
HGNC ID
HGNC:29201

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 13 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
STPyne
 Probe Info  		K84(10.00)  LDD0277  [1]
Probe 1
 Probe Info  		Y267(25.83)  LDD3495  [2]
DBIA
 Probe Info  		C36(1.80)  LDD3319  [3]
BTD
 Probe Info  		C36(1.43)  LDD2090  [4]
5E-2FA
 Probe Info  		H242(0.00); H109(0.00)  LDD2235  [5]
ATP probe
 Probe Info  		K79(0.00); K127(0.00)  LDD0199  [6]
m-APA
 Probe Info  		H242(0.00); H109(0.00)  LDD2231  [5]
4-Iodoacetamidophenylacetylene
 Probe Info  		N.A.  LDD0038  [7]
IA-alkyne
 Probe Info  		N.A.  LDD0036  [7]
Lodoacetamide azide
 Probe Info  		N.A.  LDD0037  [7]
IPM
 Probe Info  		N.A.  LDD0025  [8]
Phosphinate-6
 Probe Info  		N.A.  LDD0018  [9]
Acrolein
 Probe Info  		N.A.  LDD0217  [10]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0519  1-(6-methoxy-3,4-dihydroquinolin-1(2H)-yl)-2-nitroethan-1-one MDA-MB-231 C36(0.47)  LDD2112  [4]
 LDCM0558  2-Cyano-N-phenylacetamide MDA-MB-231 C36(0.69)  LDD2152  [4]
 LDCM0510  3-(4-(Hydroxydiphenylmethyl)piperidin-1-yl)-3-oxopropanenitrile MDA-MB-231 C36(1.05)  LDD2103  [4]
 LDCM0215  AC10 HEK-293T C36(1.00)  LDD1508  [11]
 LDCM0237  AC12 HEK-293T C36(0.99)  LDD1510  [11]
 LDCM0277  AC18 HEK-293T C36(0.94)  LDD1516  [11]
 LDCM0279  AC2 HEK-293T C36(1.01)  LDD1518  [11]
 LDCM0280  AC20 HEK-293T C36(0.91)  LDD1519  [11]
 LDCM0281  AC21 HEK-293T C36(1.11)  LDD1520  [11]
 LDCM0286  AC26 HEK-293T C36(0.86)  LDD1525  [11]
 LDCM0288  AC28 HEK-293T C36(0.95)  LDD1527  [11]
 LDCM0289  AC29 HEK-293T C36(1.04)  LDD1528  [11]
 LDCM0295  AC34 HEK-293T C36(1.04)  LDD1534  [11]
 LDCM0297  AC36 HEK-293T C36(0.96)  LDD1536  [11]
 LDCM0298  AC37 HEK-293T C36(1.09)  LDD1537  [11]
 LDCM0301  AC4 HEK-293T C36(0.91)  LDD1540  [11]
 LDCM0304  AC42 HEK-293T C36(0.88)  LDD1543  [11]
 LDCM0306  AC44 HEK-293T C36(0.97)  LDD1545  [11]
 LDCM0307  AC45 HEK-293T C36(1.03)  LDD1546  [11]
 LDCM0312  AC5 HEK-293T C36(1.11)  LDD1551  [11]
 LDCM0313  AC50 HEK-293T C36(1.12)  LDD1552  [11]
 LDCM0315  AC52 HEK-293T C36(0.99)  LDD1554  [11]
 LDCM0316  AC53 HEK-293T C36(1.16)  LDD1555  [11]
 LDCM0321  AC58 HEK-293T C36(0.94)  LDD1560  [11]
 LDCM0324  AC60 HEK-293T C36(0.89)  LDD1563  [11]
 LDCM0325  AC61 HEK-293T C36(1.05)  LDD1564  [11]
 LDCM0520  AKOS000195272 MDA-MB-231 C36(0.91)  LDD2113  [4]
 LDCM0248  AKOS034007472 HEK-293T C36(1.07)  LDD1511  [11]
 LDCM0369  CL100 HEK-293T C36(0.98)  LDD1573  [11]
 LDCM0373  CL104 HEK-293T C36(0.98)  LDD1577  [11]
 LDCM0377  CL108 HEK-293T C36(1.03)  LDD1581  [11]
 LDCM0382  CL112 HEK-293T C36(1.00)  LDD1586  [11]
 LDCM0386  CL116 HEK-293T C36(1.04)  LDD1590  [11]
 LDCM0391  CL120 HEK-293T C36(1.02)  LDD1595  [11]
 LDCM0395  CL124 HEK-293T C36(1.10)  LDD1599  [11]
 LDCM0399  CL128 HEK-293T C36(0.98)  LDD1603  [11]
 LDCM0403  CL16 HEK-293T C36(0.99)  LDD1607  [11]
 LDCM0405  CL18 HEK-293T C36(0.85)  LDD1609  [11]
 LDCM0408  CL20 HEK-293T C36(0.70)  LDD1612  [11]
 LDCM0409  CL21 HEK-293T C36(0.82)  LDD1613  [11]
 LDCM0416  CL28 HEK-293T C36(1.09)  LDD1620  [11]
 LDCM0419  CL30 HEK-293T C36(0.95)  LDD1623  [11]
 LDCM0421  CL32 HEK-293T C36(0.76)  LDD1625  [11]
 LDCM0422  CL33 HEK-293T C36(0.78)  LDD1626  [11]
 LDCM0429  CL4 HEK-293T C36(1.04)  LDD1633  [11]
 LDCM0430  CL40 HEK-293T C36(1.01)  LDD1634  [11]
 LDCM0432  CL42 HEK-293T C36(0.91)  LDD1636  [11]
 LDCM0434  CL44 HEK-293T C36(0.82)  LDD1638  [11]
 LDCM0435  CL45 HEK-293T C36(0.80)  LDD1639  [11]
 LDCM0443  CL52 HEK-293T C36(1.03)  LDD1646  [11]
 LDCM0445  CL54 HEK-293T C36(0.90)  LDD1648  [11]
 LDCM0447  CL56 HEK-293T C36(0.73)  LDD1650  [11]
 LDCM0448  CL57 HEK-293T C36(0.81)  LDD1651  [11]
 LDCM0451  CL6 HEK-293T C36(0.82)  LDD1654  [11]
 LDCM0456  CL64 HEK-293T C36(0.99)  LDD1659  [11]
 LDCM0458  CL66 HEK-293T C36(0.87)  LDD1661  [11]
 LDCM0460  CL68 HEK-293T C36(0.79)  LDD1663  [11]
 LDCM0461  CL69 HEK-293T C36(0.92)  LDD1664  [11]
 LDCM0469  CL76 HEK-293T C36(1.09)  LDD1672  [11]
 LDCM0471  CL78 HEK-293T C36(0.94)  LDD1674  [11]
 LDCM0473  CL8 HEK-293T C36(0.50)  LDD1676  [11]
 LDCM0474  CL80 HEK-293T C36(0.91)  LDD1677  [11]
 LDCM0475  CL81 HEK-293T C36(0.91)  LDD1678  [11]
 LDCM0482  CL88 HEK-293T C36(1.11)  LDD1685  [11]
 LDCM0484  CL9 HEK-293T C36(0.80)  LDD1687  [11]
 LDCM0485  CL90 HEK-293T C36(0.82)  LDD1688  [11]
 LDCM0487  CL92 HEK-293T C36(0.89)  LDD1690  [11]
 LDCM0488  CL93 HEK-293T C36(0.91)  LDD1691  [11]
 LDCM0213  Electrophilic fragment 2 MDA-MB-231 C36(0.88)  LDD1702  [4]
 LDCM0107  IAA HeLa N.A.  LDD0221  [10]
 LDCM0022  KB02 HEK-293T C36(1.10)  LDD1492  [11]
 LDCM0023  KB03 HEK-293T C36(1.04)  LDD1497  [11]
 LDCM0024  KB05 MEWO C36(1.80)  LDD3319  [3]
 LDCM0109  NEM HeLa N.A.  LDD0227  [10]
 LDCM0497  Nucleophilic fragment 11b MDA-MB-231 C36(1.43)  LDD2090  [4]
 LDCM0501  Nucleophilic fragment 13b MDA-MB-231 C36(1.10)  LDD2094  [4]
 LDCM0504  Nucleophilic fragment 15a MDA-MB-231 C36(0.64)  LDD2097  [4]
 LDCM0505  Nucleophilic fragment 15b MDA-MB-231 C36(0.89)  LDD2098  [4]
 LDCM0506  Nucleophilic fragment 16a MDA-MB-231 C36(1.02)  LDD2099  [4]
 LDCM0511  Nucleophilic fragment 18b MDA-MB-231 C36(0.65)  LDD2104  [4]
 LDCM0514  Nucleophilic fragment 20a MDA-MB-231 C36(1.16)  LDD2107  [4]
 LDCM0515  Nucleophilic fragment 20b MDA-MB-231 C36(0.40)  LDD2108  [4]
 LDCM0516  Nucleophilic fragment 21a MDA-MB-231 C36(0.66)  LDD2109  [4]
 LDCM0521  Nucleophilic fragment 23b MDA-MB-231 C36(0.56)  LDD2114  [4]
 LDCM0523  Nucleophilic fragment 24b MDA-MB-231 C36(0.99)  LDD2116  [4]
 LDCM0527  Nucleophilic fragment 26b MDA-MB-231 C36(0.98)  LDD2120  [4]
 LDCM0529  Nucleophilic fragment 27b MDA-MB-231 C36(0.76)  LDD2122  [4]
 LDCM0534  Nucleophilic fragment 30a MDA-MB-231 C36(0.91)  LDD2127  [4]
 LDCM0536  Nucleophilic fragment 31 MDA-MB-231 C36(1.31)  LDD2129  [4]
 LDCM0541  Nucleophilic fragment 36 MDA-MB-231 C36(0.53)  LDD2134  [4]
 LDCM0543  Nucleophilic fragment 38 MDA-MB-231 C36(1.17)  LDD2136  [4]
 LDCM0544  Nucleophilic fragment 39 MDA-MB-231 C36(0.97)  LDD2137  [4]
 LDCM0546  Nucleophilic fragment 40 MDA-MB-231 C36(0.85)  LDD2140  [4]
 LDCM0547  Nucleophilic fragment 41 MDA-MB-231 C36(0.34)  LDD2141  [4]
 LDCM0549  Nucleophilic fragment 43 MDA-MB-231 C36(0.99)  LDD2143  [4]
 LDCM0550  Nucleophilic fragment 5a MDA-MB-231 C36(1.97)  LDD2144  [4]
 LDCM0551  Nucleophilic fragment 5b MDA-MB-231 C36(1.75)  LDD2145  [4]
 LDCM0552  Nucleophilic fragment 6a MDA-MB-231 C36(0.79)  LDD2146  [4]
 LDCM0553  Nucleophilic fragment 6b MDA-MB-231 C36(2.51)  LDD2147  [4]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 8 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex, mitochondrial (DLST) 2-oxoacid dehydrogenase family P36957
Phenylalanine--tRNA ligase, mitochondrial (FARS2) Class-II aminoacyl-tRNA synthetase family O95363
RNA helicase aquarius (AQR) CWF11 family O60306
ATP-dependent RNA helicase DHX8 (DHX8) DEAD box helicase family Q14562
Nucleoside diphosphate kinase homolog 7 (NME7) NDK family Q9Y5B8
Serine/threonine-protein kinase Nek7 (NEK7) NEK Ser/Thr protein kinase family Q8TDX7
TGF-beta receptor type-2 (TGFBR2) TKL Ser/Thr protein kinase family P37173
Thiosulfate sulfurtransferase/rhodanese-like domain-containing protein 2 (TSTD2) . Q5T7W7
Transporter and channel
Click To Hide/Show 3 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Calreticulin (CALR) Calreticulin family P27797
Exocyst complex component 8 (EXOC8) EXO84 family Q8IYI6
Nuclear RNA export factor 1 (NXF1) NXF family Q9UBU9
Transcription factor
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Zinc finger protein 587 (ZNF587) Krueppel C2H2-type zinc-finger protein family Q96SQ5
Zinc finger protein 629 (ZNF629) Krueppel C2H2-type zinc-finger protein family Q9UEG4
Other
Click To Hide/Show 10 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Porimin (TMEM123) CD164 family Q8N131
Pre-mRNA-splicing factor SYF1 (XAB2) Crooked-neck family Q9HCS7
Cyclin-C (CCNC) Cyclin family P24863
DNA mismatch repair protein Mlh1 (MLH1) DNA mismatch repair MutL/HexB family P40692
Protein FAM161B (FAM161B) FAM161 family Q96MY7
Protein FAM90A1 (FAM90A1) FAM90 family Q86YD7
Coiled-coil domain-containing protein 185 (CCDC185) . Q8N715
Fibroblast growth factor receptor substrate 3 (FRS3) . O43559
Leukocyte receptor cluster member 1 (LENG1) . Q96BZ8
Rhombotin-1 (LMO1) . P25800

References

1 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
2 An Azo Coupling-Based Chemoproteomic Approach to Systematically Profile the Tyrosine Reactivity in the Human Proteome. Anal Chem. 2021 Jul 27;93(29):10334-10342. doi: 10.1021/acs.analchem.1c01935. Epub 2021 Jul 12.
3 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
4 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
5 Global profiling of functional histidines in live cells using small-molecule photosensitizer and chemical probe relay labelling. Nat Chem. 2024 Jun 4. doi: 10.1038/s41557-024-01545-6. Online ahead of print.
Mass spectrometry data entry: PXD042377
6 Targeted Proteomic Approaches for Proteome-Wide Characterizations of the AMP-Binding Capacities of Kinases. J Proteome Res. 2022 Aug 5;21(8):2063-2070. doi: 10.1021/acs.jproteome.2c00225. Epub 2022 Jul 12.
7 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
8 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
9 DFT-Guided Discovery of Ethynyl-Triazolyl-Phosphinates as Modular Electrophiles for Chemoselective Cysteine Bioconjugation and Profiling. Angew Chem Int Ed Engl. 2022 Oct 10;61(41):e202205348. doi: 10.1002/anie.202205348. Epub 2022 Aug 22.
Mass spectrometry data entry: PXD033004
10 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
11 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402