Details of the Target

General Information of Target

Target ID LDTP13161
Target Name Protein DBF4 homolog A (DBF4)
Gene Name DBF4
Gene ID 10926
Synonyms
ASK; DBF4A; ZDBF1; Protein DBF4 homolog A; Activator of S phase kinase; Chiffon homolog A; DBF4-type zinc finger-containing protein 1
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MPSPGTVCSLLLLGMLWLDLAMAGSSFLSPEHQRVQQRKESKKPPAKLQPRALAGWLRPE
DGGQAEGAEDELEVRFNAPFDVGIKLSGVQYQQHSQALGKFLQDILWEEAKEAPADK
Target Bioclass
Other
Subcellular location
Nucleus
Function
Regulatory subunit for CDC7 which activates its kinase activity thereby playing a central role in DNA replication and cell proliferation. Required for progression of S phase. The complex CDC7-DBF4A selectively phosphorylates MCM2 subunit at 'Ser-40' and 'Ser-53' and then is involved in regulating the initiation of DNA replication during cell cycle.
Uniprot ID
Q9UBU7
Ensemble ID
ENST00000265728.6
HGNC ID
HGNC:17364
ChEMBL ID
CHEMBL4483

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
AN3CA Deletion: p.F663SfsTer15 .
CCK81 Deletion: p.N25TfsTer5 .
GB1 SNV: p.S525Ter .
HS578T SNV: p.K408M .
LOXIMVI SNV: p.Y353N .
MCC26 SNV: p.K516M .
MOLT4 Deletion: p.K408SfsTer16 .
MSTO211H SNV: p.H552R .
SUIT2 SNV: p.I444V .
SW480 SNV: p.E653Ter .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 3 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
IPM
 Probe Info  		C43(3.06)  LDD1702  [1]
DBIA
 Probe Info  		C135(1.40)  LDD0532  [2]
SF
 Probe Info  		N.A.  LDD0028  [3]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0214  AC1 HEK-293T C135(1.07); C250(1.08)  LDD1507  [4]
 LDCM0215  AC10 HCT 116 C135(1.40)  LDD0532  [2]
 LDCM0216  AC100 HCT 116 C135(1.42)  LDD0533  [2]
 LDCM0217  AC101 HCT 116 C135(1.77)  LDD0534  [2]
 LDCM0218  AC102 HCT 116 C135(1.65)  LDD0535  [2]
 LDCM0219  AC103 HCT 116 C135(1.59)  LDD0536  [2]
 LDCM0220  AC104 HCT 116 C135(1.08)  LDD0537  [2]
 LDCM0221  AC105 HCT 116 C135(1.31)  LDD0538  [2]
 LDCM0222  AC106 HCT 116 C135(1.54)  LDD0539  [2]
 LDCM0223  AC107 HCT 116 C135(2.01)  LDD0540  [2]
 LDCM0224  AC108 HCT 116 C135(0.95)  LDD0541  [2]
 LDCM0225  AC109 HCT 116 C135(1.89)  LDD0542  [2]
 LDCM0226  AC11 HCT 116 C135(1.77)  LDD0543  [2]
 LDCM0227  AC110 HCT 116 C135(1.31)  LDD0544  [2]
 LDCM0228  AC111 HCT 116 C135(1.27)  LDD0545  [2]
 LDCM0229  AC112 HCT 116 C135(1.11)  LDD0546  [2]
 LDCM0237  AC12 HCT 116 C135(1.04)  LDD0554  [2]
 LDCM0259  AC14 HCT 116 C135(1.45)  LDD0576  [2]
 LDCM0263  AC143 HCT 116 C135(0.86)  LDD0580  [2]
 LDCM0264  AC144 HCT 116 C135(0.96)  LDD0581  [2]
 LDCM0265  AC145 HCT 116 C135(0.93)  LDD0582  [2]
 LDCM0266  AC146 HCT 116 C135(1.49)  LDD0583  [2]
 LDCM0267  AC147 HCT 116 C135(1.11)  LDD0584  [2]
 LDCM0268  AC148 HCT 116 C135(1.83)  LDD0585  [2]
 LDCM0269  AC149 HCT 116 C135(1.69)  LDD0586  [2]
 LDCM0270  AC15 HCT 116 C135(1.40)  LDD0587  [2]
 LDCM0271  AC150 HCT 116 C135(1.24)  LDD0588  [2]
 LDCM0272  AC151 HCT 116 C135(1.12)  LDD0589  [2]
 LDCM0273  AC152 HCT 116 C135(1.40)  LDD0590  [2]
 LDCM0274  AC153 HCT 116 C135(1.34)  LDD0591  [2]
 LDCM0621  AC154 HCT 116 C135(1.08)  LDD2158  [2]
 LDCM0622  AC155 HCT 116 C135(1.13)  LDD2159  [2]
 LDCM0623  AC156 HCT 116 C135(1.02)  LDD2160  [2]
 LDCM0624  AC157 HCT 116 C135(1.06)  LDD2161  [2]
 LDCM0276  AC17 HEK-293T C135(1.07); C250(1.08)  LDD1515  [4]
 LDCM0282  AC22 HEK-293T C250(0.93)  LDD1521  [4]
 LDCM0284  AC24 HEK-293T C250(0.91)  LDD1523  [4]
 LDCM0285  AC25 HEK-293T C135(1.01); C250(1.18)  LDD1524  [4]
 LDCM0291  AC30 HEK-293T C250(1.02)  LDD1530  [4]
 LDCM0293  AC32 HEK-293T C250(1.01)  LDD1532  [4]
 LDCM0294  AC33 HEK-293T C135(1.06); C250(1.04)  LDD1533  [4]
 LDCM0299  AC38 HEK-293T C250(1.05)  LDD1538  [4]
 LDCM0302  AC40 HEK-293T C250(0.99)  LDD1541  [4]
 LDCM0303  AC41 HEK-293T C135(1.09); C250(1.27)  LDD1542  [4]
 LDCM0308  AC46 HEK-293T C250(1.26)  LDD1547  [4]
 LDCM0310  AC48 HEK-293T C250(1.00)  LDD1549  [4]
 LDCM0311  AC49 HEK-293T C135(1.09); C250(1.41)  LDD1550  [4]
 LDCM0317  AC54 HEK-293T C250(1.07)  LDD1556  [4]
 LDCM0319  AC56 HEK-293T C250(1.12)  LDD1558  [4]
 LDCM0320  AC57 HEK-293T C135(1.05); C250(1.30)  LDD1559  [4]
 LDCM0323  AC6 HCT 116 C135(1.45)  LDD0640  [2]
 LDCM0326  AC62 HEK-293T C250(1.08)  LDD1565  [4]
 LDCM0328  AC64 HEK-293T C250(0.83)  LDD1567  [4]
 LDCM0332  AC68 HCT 116 C135(1.77)  LDD0649  [2]
 LDCM0333  AC69 HCT 116 C135(0.79)  LDD0650  [2]
 LDCM0334  AC7 HCT 116 C135(1.64)  LDD0651  [2]
 LDCM0335  AC70 HCT 116 C135(0.68)  LDD0652  [2]
 LDCM0336  AC71 HCT 116 C135(0.84)  LDD0653  [2]
 LDCM0337  AC72 HCT 116 C135(0.90)  LDD0654  [2]
 LDCM0338  AC73 HCT 116 C135(0.95)  LDD0655  [2]
 LDCM0339  AC74 HCT 116 C135(0.79)  LDD0656  [2]
 LDCM0340  AC75 HCT 116 C135(0.81)  LDD0657  [2]
 LDCM0341  AC76 HCT 116 C135(0.70)  LDD0658  [2]
 LDCM0342  AC77 HCT 116 C135(0.87)  LDD0659  [2]
 LDCM0343  AC78 HCT 116 C135(0.72)  LDD0660  [2]
 LDCM0344  AC79 HCT 116 C135(0.93)  LDD0661  [2]
 LDCM0345  AC8 HCT 116 C135(1.51)  LDD0662  [2]
 LDCM0346  AC80 HCT 116 C135(0.66)  LDD0663  [2]
 LDCM0347  AC81 HCT 116 C135(0.70)  LDD0664  [2]
 LDCM0348  AC82 HCT 116 C135(0.68)  LDD0665  [2]
 LDCM0349  AC83 HCT 116 C135(1.56)  LDD0666  [2]
 LDCM0350  AC84 HCT 116 C135(1.47)  LDD0667  [2]
 LDCM0351  AC85 HCT 116 C135(1.28)  LDD0668  [2]
 LDCM0352  AC86 HCT 116 C135(1.12)  LDD0669  [2]
 LDCM0353  AC87 HCT 116 C135(1.19)  LDD0670  [2]
 LDCM0354  AC88 HCT 116 C135(1.13)  LDD0671  [2]
 LDCM0355  AC89 HCT 116 C135(1.51)  LDD0672  [2]
 LDCM0357  AC90 HCT 116 C135(1.03)  LDD0674  [2]
 LDCM0358  AC91 HCT 116 C135(1.37)  LDD0675  [2]
 LDCM0359  AC92 HCT 116 C135(1.88)  LDD0676  [2]
 LDCM0360  AC93 HCT 116 C135(1.46)  LDD0677  [2]
 LDCM0361  AC94 HCT 116 C135(1.22)  LDD0678  [2]
 LDCM0362  AC95 HCT 116 C135(1.27)  LDD0679  [2]
 LDCM0363  AC96 HCT 116 C135(1.23)  LDD0680  [2]
 LDCM0364  AC97 HCT 116 C135(1.38)  LDD0681  [2]
 LDCM0365  AC98 HCT 116 C135(1.18)  LDD0682  [2]
 LDCM0366  AC99 HCT 116 C135(1.70)  LDD0683  [2]
 LDCM0248  AKOS034007472 HCT 116 C135(1.05)  LDD0565  [2]
 LDCM0356  AKOS034007680 HCT 116 C135(1.05)  LDD0673  [2]
 LDCM0275  AKOS034007705 HCT 116 C135(1.63)  LDD0592  [2]
 LDCM0367  CL1 HCT 116 C135(1.21)  LDD0684  [2]
 LDCM0368  CL10 HCT 116 C135(0.78)  LDD0685  [2]
 LDCM0369  CL100 HEK-293T C250(1.03)  LDD1573  [4]
 LDCM0370  CL101 HCT 116 C135(0.91)  LDD0687  [2]
 LDCM0371  CL102 HCT 116 C135(0.98)  LDD0688  [2]
 LDCM0372  CL103 HCT 116 C135(1.00)  LDD0689  [2]
 LDCM0373  CL104 HCT 116 C135(1.16)  LDD0690  [2]
 LDCM0374  CL105 HEK-293T C135(1.18)  LDD1578  [4]
 LDCM0377  CL108 HEK-293T C250(1.08)  LDD1581  [4]
 LDCM0378  CL109 HEK-293T C135(1.18)  LDD1582  [4]
 LDCM0379  CL11 HCT 116 C135(0.80)  LDD0696  [2]
 LDCM0382  CL112 HEK-293T C250(1.10)  LDD1586  [4]
 LDCM0383  CL113 HEK-293T C135(1.04)  LDD1587  [4]
 LDCM0386  CL116 HEK-293T C250(1.02)  LDD1590  [4]
 LDCM0387  CL117 HEK-293T C135(1.10)  LDD1591  [4]
 LDCM0390  CL12 HCT 116 C135(0.86)  LDD0707  [2]
 LDCM0391  CL120 HEK-293T C250(1.07)  LDD1595  [4]
 LDCM0392  CL121 HEK-293T C135(0.94)  LDD1596  [4]
 LDCM0395  CL124 HEK-293T C250(1.24)  LDD1599  [4]
 LDCM0396  CL125 HEK-293T C135(1.13)  LDD1600  [4]
 LDCM0399  CL128 HEK-293T C250(1.06)  LDD1603  [4]
 LDCM0400  CL13 HCT 116 C135(1.03)  LDD0717  [2]
 LDCM0401  CL14 HCT 116 C135(1.08)  LDD0718  [2]
 LDCM0402  CL15 HCT 116 C135(1.05)  LDD0719  [2]
 LDCM0403  CL16 HEK-293T C250(1.28)  LDD1607  [4]
 LDCM0404  CL17 HEK-293T C135(1.01); C250(1.19)  LDD1608  [4]
 LDCM0407  CL2 HCT 116 C135(1.11)  LDD0724  [2]
 LDCM0410  CL22 HEK-293T C250(1.29)  LDD1614  [4]
 LDCM0412  CL24 HEK-293T C250(1.21)  LDD1616  [4]
 LDCM0413  CL25 HEK-293T C135(1.12)  LDD1617  [4]
 LDCM0416  CL28 HEK-293T C250(1.16)  LDD1620  [4]
 LDCM0417  CL29 HEK-293T C135(1.05); C250(1.08)  LDD1621  [4]
 LDCM0418  CL3 HCT 116 C135(1.36)  LDD0735  [2]
 LDCM0423  CL34 HEK-293T C250(1.17)  LDD1627  [4]
 LDCM0425  CL36 HEK-293T C250(0.91)  LDD1629  [4]
 LDCM0426  CL37 HEK-293T C135(1.28)  LDD1630  [4]
 LDCM0429  CL4 HCT 116 C135(0.94)  LDD0746  [2]
 LDCM0430  CL40 HEK-293T C250(1.03)  LDD1634  [4]
 LDCM0431  CL41 HEK-293T C135(0.88); C250(1.09)  LDD1635  [4]
 LDCM0436  CL46 HEK-293T C250(0.98)  LDD1640  [4]
 LDCM0438  CL48 HEK-293T C250(0.92)  LDD1642  [4]
 LDCM0439  CL49 HEK-293T C135(0.85)  LDD1643  [4]
 LDCM0440  CL5 HCT 116 C135(1.11)  LDD0757  [2]
 LDCM0443  CL52 HEK-293T C250(1.02)  LDD1646  [4]
 LDCM0444  CL53 HEK-293T C135(0.92); C250(1.36)  LDD1647  [4]
 LDCM0449  CL58 HEK-293T C250(1.12)  LDD1652  [4]
 LDCM0451  CL6 HCT 116 C135(1.17)  LDD0768  [2]
 LDCM0452  CL60 HEK-293T C250(0.90)  LDD1655  [4]
 LDCM0453  CL61 HEK-293T C135(0.78)  LDD1656  [4]
 LDCM0456  CL64 HEK-293T C250(1.28)  LDD1659  [4]
 LDCM0457  CL65 HEK-293T C135(1.11); C250(1.37)  LDD1660  [4]
 LDCM0462  CL7 HCT 116 C135(1.10)  LDD0779  [2]
 LDCM0463  CL70 HEK-293T C250(1.13)  LDD1666  [4]
 LDCM0465  CL72 HEK-293T C250(1.04)  LDD1668  [4]
 LDCM0466  CL73 HEK-293T C135(1.26)  LDD1669  [4]
 LDCM0469  CL76 HEK-293T C250(1.08)  LDD1672  [4]
 LDCM0470  CL77 HEK-293T C135(0.98); C250(1.57)  LDD1673  [4]
 LDCM0473  CL8 HCT 116 C135(1.25)  LDD0790  [2]
 LDCM0476  CL82 HEK-293T C250(1.07)  LDD1679  [4]
 LDCM0478  CL84 HEK-293T C250(0.95)  LDD1681  [4]
 LDCM0479  CL85 HEK-293T C135(0.85)  LDD1682  [4]
 LDCM0482  CL88 HEK-293T C250(1.15)  LDD1685  [4]
 LDCM0483  CL89 HEK-293T C135(1.10); C250(1.63)  LDD1686  [4]
 LDCM0484  CL9 HCT 116 C135(0.98)  LDD0801  [2]
 LDCM0489  CL94 HEK-293T C250(1.07)  LDD1692  [4]
 LDCM0491  CL96 HEK-293T C250(1.05)  LDD1694  [4]
 LDCM0492  CL97 HEK-293T C135(0.88)  LDD1695  [4]
 LDCM0213  Electrophilic fragment 2 MDA-MB-231 C43(3.06)  LDD1702  [1]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 3 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
60 kDa heat shock protein, mitochondrial (HSPD1) Chaperonin (HSP60) family P10809
Bleomycin hydrolase (BLMH) Peptidase C1 family Q13867
Cell division cycle 7-related protein kinase (CDC7) Ser/Thr protein kinase family O00311
Transporter and channel
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Neuronal acetylcholine receptor subunit alpha-7 (CHRNA7) Ligand-gated ion channel family P36544
Other
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Glial fibrillary acidic protein (GFAP) Intermediate filament family P14136

References

1 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
2 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
3 Solid Phase Synthesis of Fluorosulfate Containing Macrocycles for Chemoproteomic Workflows. bioRxiv [Preprint]. 2023 Feb 18:2023.02.17.529022. doi: 10.1101/2023.02.17.529022.
Mass spectrometry data entry: PXD039931
4 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402