Details of the Target

General Information of Target

Target ID LDTP13050
Target Name Kelch-like protein 8 (KLHL8)
Gene Name KLHL8
Gene ID 57563
Synonyms
KIAA1378; Kelch-like protein 8
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MAASLSERLFSLELLVDWVRLEARLLPSPAAAVEQEEEEEEKEQGEASSPRGLCPAVAFR
LLDFPTLLVYPPDGPGAPAAEPWPGVIRFGRGKSCLFRLQPATLHCRLLRTPLATLLLQL
PPGRPTPTPQLLGACDISLATAAHRVVGPAASGCSHRHRGRFPLHNRVGERTGDIALAYR
LTDLGSRLLSQLERPLTFTRTGGGAEVSPQTQQERQQLQQPASQPSPKEADKPLGELEIP
EAQKDLKEMVKSKAECDNVGSVENGKTNSVVTCSGAGNGRNVSSLNEEVTELDMETNIFC
PPPLYYTNLTQEKPPPAQAKITIEPQMNAPEEMDDASPEKKRVNPPAHRSCLKHPSSAAH
EHPPMLVNPPHIQNIGATNQTCQTEQNRINTIRQLPLLNALLVELSLLYDQPVTSPAHIH
PHLAWLYRTEDKKSPESSAKSTCRSEAKKDKRSVGGCEKSVSLQYKKNQIENYKEDKYSE
KSSGALHKRVPKGRLLYGLTNTLRLRLKLTNPDMLVVHEKRELYRKRQSQMLGTKFRIPS
SKVKLLSSAEQSQKPQLPEDKYLDSDASFTENSDTSRQISGVFDEPSTSKETKLKYATEK
KTVDCSKNRINNVSLEEVVSPANSIIPERLTPTNILGGNVEMKIQSPCVFQQDAVVDRIV
DKEIDIRQVKTTDNDILMADISDKRTGKNSCYENISELKYSDDLSSPCYSEDFCTSEDTS
RSFKAHDSSSRTENPKHSQYTSKSSDTGVSKKKNSSDRSSILSPPFSAGSPVHSYRKFHI
SKTQDKSLEEASSISASDLSSTHWTEQKENQIDQNSMHNSEITKRAQDISVKTRSSWKSL
EKSQSPQTSQVSSYLPSNVSELNVLDSSTSDHFEEGNDDVGSLNISKQCKDICELVINKL
PGYTM
Target Bioclass
Other
Function Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex required for The BCR(KLHL8) ubiquitin ligase complex mediates ubiquitination and degradation of RAPSN.
Uniprot ID
Q9P2G9
Ensemble ID
ENST00000273963.10
HGNC ID
HGNC:18644

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
HCT116 SNV: p.M399T DBIA    Probe Info 
Ishikawa (Heraklio) 02 ER SNV: p.N569D .
MKN1 SNV: p.H54R .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 3 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
BTD
 Probe Info  		C599(0.63)  LDD2148  [1]
DBIA
 Probe Info  		C590(2.38)  LDD0080  [2]
AOyne
 Probe Info  		14.30  LDD0443  [3]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0214  AC1 HCT 116 C599(1.20); C601(1.20)  LDD0531  [2]
 LDCM0230  AC113 HCT 116 C599(0.89); C601(0.89)  LDD0547  [2]
 LDCM0231  AC114 HCT 116 C599(0.88); C601(0.88)  LDD0548  [2]
 LDCM0232  AC115 HCT 116 C599(0.93); C601(0.93)  LDD0549  [2]
 LDCM0233  AC116 HCT 116 C599(0.91); C601(0.91)  LDD0550  [2]
 LDCM0234  AC117 HCT 116 C599(1.00); C601(1.00)  LDD0551  [2]
 LDCM0235  AC118 HCT 116 C599(1.11); C601(1.11)  LDD0552  [2]
 LDCM0236  AC119 HCT 116 C599(1.03); C601(1.03)  LDD0553  [2]
 LDCM0238  AC120 HCT 116 C599(1.17); C601(1.17)  LDD0555  [2]
 LDCM0239  AC121 HCT 116 C599(0.93); C601(0.93)  LDD0556  [2]
 LDCM0240  AC122 HCT 116 C599(1.06); C601(1.06)  LDD0557  [2]
 LDCM0241  AC123 HCT 116 C599(0.85); C601(0.85)  LDD0558  [2]
 LDCM0242  AC124 HCT 116 C599(0.88); C601(0.88)  LDD0559  [2]
 LDCM0243  AC125 HCT 116 C599(0.98); C601(0.98)  LDD0560  [2]
 LDCM0244  AC126 HCT 116 C599(0.91); C601(0.91)  LDD0561  [2]
 LDCM0245  AC127 HCT 116 C599(1.07); C601(1.07)  LDD0562  [2]
 LDCM0263  AC143 HCT 116 C599(0.95); C601(0.95)  LDD0580  [2]
 LDCM0264  AC144 HCT 116 C599(1.03); C601(1.03)  LDD0581  [2]
 LDCM0265  AC145 HCT 116 C599(0.96); C601(0.96)  LDD0582  [2]
 LDCM0266  AC146 HCT 116 C599(0.95); C601(0.95)  LDD0583  [2]
 LDCM0267  AC147 HCT 116 C599(0.97); C601(0.97)  LDD0584  [2]
 LDCM0268  AC148 HCT 116 C599(1.02); C601(1.02)  LDD0585  [2]
 LDCM0269  AC149 HCT 116 C599(1.08); C601(1.08)  LDD0586  [2]
 LDCM0271  AC150 HCT 116 C599(1.07); C601(1.07)  LDD0588  [2]
 LDCM0272  AC151 HCT 116 C599(1.00); C601(1.00)  LDD0589  [2]
 LDCM0273  AC152 HCT 116 C599(0.83); C601(0.83)  LDD0590  [2]
 LDCM0274  AC153 HCT 116 C599(1.00); C601(1.00)  LDD0591  [2]
 LDCM0621  AC154 HCT 116 C599(1.01); C601(1.01)  LDD2158  [2]
 LDCM0622  AC155 HCT 116 C599(1.00); C601(1.00)  LDD2159  [2]
 LDCM0623  AC156 HCT 116 C599(1.05); C601(1.05)  LDD2160  [2]
 LDCM0624  AC157 HCT 116 C599(0.98); C601(0.98)  LDD2161  [2]
 LDCM0279  AC2 HCT 116 C599(1.73); C601(1.73)  LDD0596  [2]
 LDCM0290  AC3 HCT 116 C599(1.64); C601(1.64)  LDD0607  [2]
 LDCM0296  AC35 HCT 116 C599(0.82); C601(0.82)  LDD0613  [2]
 LDCM0297  AC36 HCT 116 C599(0.77); C601(0.77)  LDD0614  [2]
 LDCM0298  AC37 HCT 116 C599(0.99); C601(0.99)  LDD0615  [2]
 LDCM0299  AC38 HCT 116 C599(0.97); C601(0.97)  LDD0616  [2]
 LDCM0300  AC39 HCT 116 C599(0.93); C601(0.93)  LDD0617  [2]
 LDCM0301  AC4 HCT 116 C599(1.43); C601(1.43)  LDD0618  [2]
 LDCM0302  AC40 HCT 116 C599(0.93); C601(0.93)  LDD0619  [2]
 LDCM0303  AC41 HCT 116 C599(1.08); C601(1.08)  LDD0620  [2]
 LDCM0304  AC42 HCT 116 C599(0.89); C601(0.89)  LDD0621  [2]
 LDCM0305  AC43 HCT 116 C599(0.98); C601(0.98)  LDD0622  [2]
 LDCM0306  AC44 HCT 116 C599(0.89); C601(0.89)  LDD0623  [2]
 LDCM0307  AC45 HCT 116 C599(0.91); C601(0.91)  LDD0624  [2]
 LDCM0308  AC46 HCT 116 C599(0.98); C601(0.98)  LDD0625  [2]
 LDCM0309  AC47 HCT 116 C599(1.01); C601(1.01)  LDD0626  [2]
 LDCM0310  AC48 HCT 116 C599(1.31); C601(1.31)  LDD0627  [2]
 LDCM0311  AC49 HCT 116 C599(0.89); C601(0.89)  LDD0628  [2]
 LDCM0312  AC5 HCT 116 C599(1.25); C601(1.25)  LDD0629  [2]
 LDCM0313  AC50 HCT 116 C599(1.09); C601(1.09)  LDD0630  [2]
 LDCM0314  AC51 HCT 116 C599(1.21); C601(1.21)  LDD0631  [2]
 LDCM0315  AC52 HCT 116 C599(1.09); C601(1.09)  LDD0632  [2]
 LDCM0316  AC53 HCT 116 C599(0.85); C601(0.85)  LDD0633  [2]
 LDCM0317  AC54 HCT 116 C599(0.88); C601(0.88)  LDD0634  [2]
 LDCM0318  AC55 HCT 116 C599(1.11); C601(1.11)  LDD0635  [2]
 LDCM0319  AC56 HCT 116 C599(1.33); C601(1.33)  LDD0636  [2]
 LDCM0320  AC57 HCT 116 C599(1.03); C601(1.03)  LDD0637  [2]
 LDCM0321  AC58 HCT 116 C599(1.01); C601(1.01)  LDD0638  [2]
 LDCM0322  AC59 HCT 116 C599(0.86); C601(0.86)  LDD0639  [2]
 LDCM0324  AC60 HCT 116 C599(1.06); C601(1.06)  LDD0641  [2]
 LDCM0325  AC61 HCT 116 C599(1.01); C601(1.01)  LDD0642  [2]
 LDCM0326  AC62 HCT 116 C599(0.94); C601(0.94)  LDD0643  [2]
 LDCM0327  AC63 HCT 116 C599(0.92); C601(0.92)  LDD0644  [2]
 LDCM0328  AC64 HCT 116 C599(1.00); C601(1.00)  LDD0645  [2]
 LDCM0329  AC65 HCT 116 C599(0.93); C601(0.93)  LDD0646  [2]
 LDCM0330  AC66 HCT 116 C599(0.88); C601(0.88)  LDD0647  [2]
 LDCM0331  AC67 HCT 116 C599(0.80); C601(0.80)  LDD0648  [2]
 LDCM0332  AC68 HCT 116 C599(0.98); C601(0.98)  LDD0649  [2]
 LDCM0333  AC69 HCT 116 C599(0.99); C601(0.99)  LDD0650  [2]
 LDCM0335  AC70 HCT 116 C599(1.21); C601(1.21)  LDD0652  [2]
 LDCM0336  AC71 HCT 116 C599(0.96); C601(0.96)  LDD0653  [2]
 LDCM0337  AC72 HCT 116 C599(0.93); C601(0.93)  LDD0654  [2]
 LDCM0338  AC73 HCT 116 C599(1.04); C601(1.04)  LDD0655  [2]
 LDCM0339  AC74 HCT 116 C599(1.09); C601(1.09)  LDD0656  [2]
 LDCM0340  AC75 HCT 116 C599(1.24); C601(1.24)  LDD0657  [2]
 LDCM0341  AC76 HCT 116 C599(0.92); C601(0.92)  LDD0658  [2]
 LDCM0342  AC77 HCT 116 C599(0.91); C601(0.91)  LDD0659  [2]
 LDCM0343  AC78 HCT 116 C599(1.05); C601(1.05)  LDD0660  [2]
 LDCM0344  AC79 HCT 116 C599(1.06); C601(1.06)  LDD0661  [2]
 LDCM0346  AC80 HCT 116 C599(0.97); C601(0.97)  LDD0663  [2]
 LDCM0347  AC81 HCT 116 C599(0.96); C601(0.96)  LDD0664  [2]
 LDCM0348  AC82 HCT 116 C599(1.47); C601(1.47)  LDD0665  [2]
 LDCM0349  AC83 HCT 116 C599(1.33); C601(1.33)  LDD0666  [2]
 LDCM0350  AC84 HCT 116 C599(1.13); C601(1.13)  LDD0667  [2]
 LDCM0351  AC85 HCT 116 C599(1.07); C601(1.07)  LDD0668  [2]
 LDCM0352  AC86 HCT 116 C599(0.99); C601(0.99)  LDD0669  [2]
 LDCM0353  AC87 HCT 116 C599(1.12); C601(1.12)  LDD0670  [2]
 LDCM0354  AC88 HCT 116 C599(1.18); C601(1.18)  LDD0671  [2]
 LDCM0355  AC89 HCT 116 C599(1.08); C601(1.08)  LDD0672  [2]
 LDCM0357  AC90 HCT 116 C599(1.12); C601(1.12)  LDD0674  [2]
 LDCM0358  AC91 HCT 116 C599(1.18); C601(1.18)  LDD0675  [2]
 LDCM0359  AC92 HCT 116 C599(1.01); C601(1.01)  LDD0676  [2]
 LDCM0360  AC93 HCT 116 C599(1.23); C601(1.23)  LDD0677  [2]
 LDCM0361  AC94 HCT 116 C599(1.22); C601(1.22)  LDD0678  [2]
 LDCM0362  AC95 HCT 116 C599(1.09); C601(1.09)  LDD0679  [2]
 LDCM0363  AC96 HCT 116 C599(1.29); C601(1.29)  LDD0680  [2]
 LDCM0364  AC97 HCT 116 C599(1.15); C601(1.15)  LDD0681  [2]
 LDCM0369  CL100 HCT 116 C599(1.11); C601(1.11)  LDD0686  [2]
 LDCM0387  CL117 HCT 116 C599(0.99); C601(0.99)  LDD0704  [2]
 LDCM0388  CL118 HCT 116 C599(0.86); C601(0.86)  LDD0705  [2]
 LDCM0389  CL119 HCT 116 C599(0.85); C601(0.85)  LDD0706  [2]
 LDCM0391  CL120 HCT 116 C599(1.01); C601(1.01)  LDD0708  [2]
 LDCM0392  CL121 HCT 116 C599(1.10); C601(1.10)  LDD0709  [2]
 LDCM0393  CL122 HCT 116 C599(0.89); C601(0.89)  LDD0710  [2]
 LDCM0394  CL123 HCT 116 C599(1.14); C601(1.14)  LDD0711  [2]
 LDCM0395  CL124 HCT 116 C599(1.09); C601(1.09)  LDD0712  [2]
 LDCM0396  CL125 HCT 116 C599(0.90); C601(0.90)  LDD0713  [2]
 LDCM0397  CL126 HCT 116 C599(1.06); C601(1.06)  LDD0714  [2]
 LDCM0398  CL127 HCT 116 C599(0.85); C601(0.85)  LDD0715  [2]
 LDCM0399  CL128 HCT 116 C599(0.94); C601(0.94)  LDD0716  [2]
 LDCM0403  CL16 HCT 116 C599(1.00); C601(1.00)  LDD0720  [2]
 LDCM0404  CL17 HCT 116 C599(1.07); C601(1.07)  LDD0721  [2]
 LDCM0405  CL18 HCT 116 C599(1.03); C601(1.03)  LDD0722  [2]
 LDCM0406  CL19 HCT 116 C599(0.88); C601(0.88)  LDD0723  [2]
 LDCM0408  CL20 HCT 116 C599(0.96); C601(0.96)  LDD0725  [2]
 LDCM0409  CL21 HCT 116 C599(1.20); C601(1.20)  LDD0726  [2]
 LDCM0410  CL22 HCT 116 C599(1.18); C601(1.18)  LDD0727  [2]
 LDCM0411  CL23 HCT 116 C599(0.89); C601(0.89)  LDD0728  [2]
 LDCM0412  CL24 HCT 116 C599(0.98); C601(0.98)  LDD0729  [2]
 LDCM0413  CL25 HCT 116 C599(1.24); C601(1.24)  LDD0730  [2]
 LDCM0414  CL26 HCT 116 C599(0.90); C601(0.90)  LDD0731  [2]
 LDCM0415  CL27 HCT 116 C599(0.91); C601(0.91)  LDD0732  [2]
 LDCM0416  CL28 HCT 116 C599(0.85); C601(0.85)  LDD0733  [2]
 LDCM0417  CL29 HCT 116 C599(0.96); C601(0.96)  LDD0734  [2]
 LDCM0419  CL30 HCT 116 C599(0.99); C601(0.99)  LDD0736  [2]
 LDCM0469  CL76 HCT 116 C599(0.99); C601(0.99)  LDD0786  [2]
 LDCM0470  CL77 HCT 116 C599(0.98); C601(0.98)  LDD0787  [2]
 LDCM0471  CL78 HCT 116 C599(0.88); C601(0.88)  LDD0788  [2]
 LDCM0472  CL79 HCT 116 C599(1.00); C601(1.00)  LDD0789  [2]
 LDCM0474  CL80 HCT 116 C599(1.17); C601(1.17)  LDD0791  [2]
 LDCM0475  CL81 HCT 116 C599(1.13); C601(1.13)  LDD0792  [2]
 LDCM0476  CL82 HCT 116 C599(1.07); C601(1.07)  LDD0793  [2]
 LDCM0477  CL83 HCT 116 C599(1.09); C601(1.09)  LDD0794  [2]
 LDCM0478  CL84 HCT 116 C599(1.21); C601(1.21)  LDD0795  [2]
 LDCM0479  CL85 HCT 116 C599(0.98); C601(0.98)  LDD0796  [2]
 LDCM0480  CL86 HCT 116 C599(0.97); C601(0.97)  LDD0797  [2]
 LDCM0481  CL87 HCT 116 C599(1.09); C601(1.09)  LDD0798  [2]
 LDCM0482  CL88 HCT 116 C599(1.00); C601(1.00)  LDD0799  [2]
 LDCM0483  CL89 HCT 116 C599(0.90); C601(0.90)  LDD0800  [2]
 LDCM0485  CL90 HCT 116 C599(1.29); C601(1.29)  LDD0802  [2]
 LDCM0486  CL91 HCT 116 C599(1.18); C601(1.18)  LDD0803  [2]
 LDCM0487  CL92 HCT 116 C599(1.15); C601(1.15)  LDD0804  [2]
 LDCM0488  CL93 HCT 116 C599(1.52); C601(1.52)  LDD0805  [2]
 LDCM0489  CL94 HCT 116 C599(1.58); C601(1.58)  LDD0806  [2]
 LDCM0490  CL95 HCT 116 C599(1.41); C601(1.41)  LDD0807  [2]
 LDCM0491  CL96 HCT 116 C599(1.26); C601(1.26)  LDD0808  [2]
 LDCM0492  CL97 HCT 116 C599(1.69); C601(1.69)  LDD0809  [2]
 LDCM0493  CL98 HCT 116 C599(1.48); C601(1.48)  LDD0810  [2]
 LDCM0494  CL99 HCT 116 C599(1.37); C601(1.37)  LDD0811  [2]
 LDCM0022  KB02 HCT 116 C590(2.38)  LDD0080  [2]
 LDCM0023  KB03 HCT 116 C590(1.12)  LDD0081  [2]
 LDCM0024  KB05 HCT 116 C590(1.59)  LDD0082  [2]
 LDCM0554  Nucleophilic fragment 7a MDA-MB-231 C599(0.63)  LDD2148  [1]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 4 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Carbonic anhydrase-related protein (CA8) Alpha-carbonic anhydrase family P35219
Phenylethanolamine N-methyltransferase (PNMT) NNMT/PNMT/TEMT family P11086
Mitogen-activated protein kinase 9 (MAPK9) CMGC Ser/Thr protein kinase family P45984
Sulfotransferase 2B1 (SULT2B1) Sulfotransferase 1 family O00204
Other
Click To Hide/Show 3 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Death domain-associated protein 6 (DAXX) DAXX family Q9UER7
Retinaldehyde-binding protein 1 (RLBP1) . P12271
Tumor protein p53-inducible protein 13 (TP53I13) . Q8NBR0

References

1 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
2 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
3 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.