Details of the Target

General Information of Target

Target ID LDTP12751
Target Name DNA damage-inducible transcript 4 protein (DDIT4)
Gene Name DDIT4
Gene ID 54541
Synonyms
REDD1; RTP801; DNA damage-inducible transcript 4 protein; HIF-1 responsive protein RTP801; Protein regulated in development and DNA damage response 1; REDD-1
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MAASLWMGDLEPYMDENFISRAFATMGETVMSVKIIRNRLTGIPAGYCFVEFADLATAEK
CLHKINGKPLPGATPAKRFKLNYATYGKQPDNSPEYSLFVGDLTPDVDDGMLYEFFVKVY
PSCRGGKVVLDQTGVSKGYGFVKFTDELEQKRALTECQGAVGLGSKPVRLSVAIPKASRV
KPVEYSQMYSYSYNQYYQQYQNYYAQWGYDQNTGSYSYSYPQYGYTQSTMQTYEEVGDDA
LEDPMPQLDVTEANKEFMEQSEELYDALMDCHWQPLDTVSSEIPAMM
Target Type
Clinical trial
Target Bioclass
Other
Family
DDIT4 family
Subcellular location
Mitochondrion
Function
Regulates cell growth, proliferation and survival via inhibition of the activity of the mammalian target of rapamycin complex 1 (mTORC1). Inhibition of mTORC1 is mediated by a pathway that involves DDIT4/REDD1, AKT1, the TSC1-TSC2 complex and the GTPase RHEB. Plays an important role in responses to cellular energy levels and cellular stress, including responses to hypoxia and DNA damage. Regulates p53/TP53-mediated apoptosis in response to DNA damage via its effect on mTORC1 activity. Its role in the response to hypoxia depends on the cell type; it mediates mTORC1 inhibition in fibroblasts and thymocytes, but not in hepatocytes. Required for mTORC1-mediated defense against viral protein synthesis and virus replication. Inhibits neuronal differentiation and neurite outgrowth mediated by NGF via its effect on mTORC1 activity. Required for normal neuron migration during embryonic brain development. Plays a role in neuronal cell death.
TTD ID
T37245
Uniprot ID
Q9NX09
DrugMap ID
TT0AWFK
Ensemble ID
ENST00000307365.4
HGNC ID
HGNC:24944

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 3 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C140(0.94)  LDD0531  [1]
IA-alkyne
 Probe Info  		N.A.  LDD0167  [2]
TFBX
 Probe Info  		N.A.  LDD0027  [3]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0214  AC1 HCT 116 C140(0.94)  LDD0531  [1]
 LDCM0279  AC2 HCT 116 C140(1.04)  LDD0596  [1]
 LDCM0290  AC3 HCT 116 C140(0.65)  LDD0607  [1]
 LDCM0301  AC4 HCT 116 C140(0.93)  LDD0618  [1]
 LDCM0308  AC46 HCT 116 C140(1.06)  LDD0625  [1]
 LDCM0309  AC47 HCT 116 C140(0.89)  LDD0626  [1]
 LDCM0310  AC48 HCT 116 C140(0.98)  LDD0627  [1]
 LDCM0311  AC49 HCT 116 C140(1.17)  LDD0628  [1]
 LDCM0312  AC5 HCT 116 C140(0.87)  LDD0629  [1]
 LDCM0313  AC50 HCT 116 C140(1.00)  LDD0630  [1]
 LDCM0314  AC51 HCT 116 C140(0.90)  LDD0631  [1]
 LDCM0315  AC52 HCT 116 C140(1.09)  LDD0632  [1]
 LDCM0316  AC53 HCT 116 C140(0.76)  LDD0633  [1]
 LDCM0317  AC54 HCT 116 C140(0.97)  LDD0634  [1]
 LDCM0318  AC55 HCT 116 C140(1.01)  LDD0635  [1]
 LDCM0319  AC56 HCT 116 C140(0.59)  LDD0636  [1]
 LDCM0367  CL1 HCT 116 C140(0.32)  LDD0684  [1]
 LDCM0368  CL10 HCT 116 C140(0.56)  LDD0685  [1]
 LDCM0369  CL100 HCT 116 C140(1.12)  LDD0686  [1]
 LDCM0379  CL11 HCT 116 C140(0.42)  LDD0696  [1]
 LDCM0390  CL12 HCT 116 C140(1.02)  LDD0707  [1]
 LDCM0392  CL121 HCT 116 C140(0.94)  LDD0709  [1]
 LDCM0393  CL122 HCT 116 C140(0.61)  LDD0710  [1]
 LDCM0394  CL123 HCT 116 C140(0.32)  LDD0711  [1]
 LDCM0395  CL124 HCT 116 C140(0.74)  LDD0712  [1]
 LDCM0400  CL13 HCT 116 C140(1.01)  LDD0717  [1]
 LDCM0401  CL14 HCT 116 C140(0.66)  LDD0718  [1]
 LDCM0402  CL15 HCT 116 C140(0.45)  LDD0719  [1]
 LDCM0407  CL2 HCT 116 C140(0.88)  LDD0724  [1]
 LDCM0418  CL3 HCT 116 C140(1.04)  LDD0735  [1]
 LDCM0420  CL31 HCT 116 C140(0.20)  LDD0737  [1]
 LDCM0421  CL32 HCT 116 C140(0.87)  LDD0738  [1]
 LDCM0422  CL33 HCT 116 C140(0.65)  LDD0739  [1]
 LDCM0423  CL34 HCT 116 C140(0.95)  LDD0740  [1]
 LDCM0424  CL35 HCT 116 C140(1.06)  LDD0741  [1]
 LDCM0425  CL36 HCT 116 C140(0.38)  LDD0742  [1]
 LDCM0426  CL37 HCT 116 C140(1.01)  LDD0743  [1]
 LDCM0428  CL39 HCT 116 C140(0.82)  LDD0745  [1]
 LDCM0429  CL4 HCT 116 C140(0.60)  LDD0746  [1]
 LDCM0430  CL40 HCT 116 C140(0.27)  LDD0747  [1]
 LDCM0431  CL41 HCT 116 C140(0.92)  LDD0748  [1]
 LDCM0432  CL42 HCT 116 C140(0.42)  LDD0749  [1]
 LDCM0433  CL43 HCT 116 C140(1.14)  LDD0750  [1]
 LDCM0434  CL44 HCT 116 C140(0.45)  LDD0751  [1]
 LDCM0435  CL45 HCT 116 C140(0.34)  LDD0752  [1]
 LDCM0440  CL5 HCT 116 C140(0.99)  LDD0757  [1]
 LDCM0451  CL6 HCT 116 C140(0.49)  LDD0768  [1]
 LDCM0453  CL61 HCT 116 C140(1.09)  LDD0770  [1]
 LDCM0454  CL62 HCT 116 C140(0.98)  LDD0771  [1]
 LDCM0455  CL63 HCT 116 C140(0.98)  LDD0772  [1]
 LDCM0456  CL64 HCT 116 C140(1.61)  LDD0773  [1]
 LDCM0457  CL65 HCT 116 C140(0.91)  LDD0774  [1]
 LDCM0458  CL66 HCT 116 C140(1.23)  LDD0775  [1]
 LDCM0459  CL67 HCT 116 C140(0.89)  LDD0776  [1]
 LDCM0460  CL68 HCT 116 C140(0.92)  LDD0777  [1]
 LDCM0461  CL69 HCT 116 C140(1.33)  LDD0778  [1]
 LDCM0462  CL7 HCT 116 C140(1.13)  LDD0779  [1]
 LDCM0463  CL70 HCT 116 C140(1.23)  LDD0780  [1]
 LDCM0464  CL71 HCT 116 C140(1.05)  LDD0781  [1]
 LDCM0465  CL72 HCT 116 C140(1.85)  LDD0782  [1]
 LDCM0466  CL73 HCT 116 C140(1.08)  LDD0783  [1]
 LDCM0467  CL74 HCT 116 C140(1.20)  LDD0784  [1]
 LDCM0469  CL76 HCT 116 C140(1.16)  LDD0786  [1]
 LDCM0470  CL77 HCT 116 C140(0.51)  LDD0787  [1]
 LDCM0471  CL78 HCT 116 C140(1.01)  LDD0788  [1]
 LDCM0472  CL79 HCT 116 C140(1.13)  LDD0789  [1]
 LDCM0473  CL8 HCT 116 C140(1.12)  LDD0790  [1]
 LDCM0474  CL80 HCT 116 C140(1.16)  LDD0791  [1]
 LDCM0475  CL81 HCT 116 C140(1.01)  LDD0792  [1]
 LDCM0476  CL82 HCT 116 C140(1.16)  LDD0793  [1]
 LDCM0477  CL83 HCT 116 C140(0.70)  LDD0794  [1]
 LDCM0478  CL84 HCT 116 C140(0.77)  LDD0795  [1]
 LDCM0479  CL85 HCT 116 C140(0.92)  LDD0796  [1]
 LDCM0480  CL86 HCT 116 C140(0.99)  LDD0797  [1]
 LDCM0481  CL87 HCT 116 C140(0.53)  LDD0798  [1]
 LDCM0482  CL88 HCT 116 C140(1.24)  LDD0799  [1]
 LDCM0483  CL89 HCT 116 C140(1.09)  LDD0800  [1]
 LDCM0484  CL9 HCT 116 C140(0.87)  LDD0801  [1]
 LDCM0485  CL90 HCT 116 C140(0.11)  LDD0802  [1]
 LDCM0486  CL91 HCT 116 C140(0.62)  LDD0803  [1]
 LDCM0487  CL92 HCT 116 C140(0.13)  LDD0804  [1]
 LDCM0488  CL93 HCT 116 C140(0.21)  LDD0805  [1]
 LDCM0489  CL94 HCT 116 C140(0.24)  LDD0806  [1]
 LDCM0490  CL95 HCT 116 C140(0.28)  LDD0807  [1]
 LDCM0491  CL96 HCT 116 C140(0.86)  LDD0808  [1]
 LDCM0492  CL97 HCT 116 C140(0.52)  LDD0809  [1]
 LDCM0493  CL98 HCT 116 C140(0.42)  LDD0810  [1]
 LDCM0494  CL99 HCT 116 C140(0.79)  LDD0811  [1]
 LDCM0468  Fragment33 HCT 116 C140(1.12)  LDD0785  [1]
 LDCM0427  Fragment51 HCT 116 C140(0.73)  LDD0744  [1]
 LDCM0022  KB02 UACC257 C140(3.44)  LDD2491  [4]
 LDCM0023  KB03 WM115 C140(3.55)  LDD2910  [4]
 LDCM0024  KB05 UACC257 C140(3.39)  LDD3325  [4]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Ubiquitin-conjugating enzyme E2 K (UBE2K) Ubiquitin-conjugating enzyme family P61086
Transcription factor
Click To Hide/Show 3 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Krueppel-like factor 3 (KLF3) Krueppel C2H2-type zinc-finger protein family P57682
Visual system homeobox 2 (VSX2) Paired homeobox family P58304
Forkhead box protein D4-like 6 (FOXD4L6) . Q3SYB3
Immunoglobulin
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Platelet endothelial cell adhesion molecule (PECAM1) . P16284
Other
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Kelch-like ECH-associated protein 1 (KEAP1) KEAP1 family Q14145
Polyamine-modulated factor 1 (PMF1) . Q6P1K2

The Drug(s) Related To This Target

Phase 2
Click To Hide/Show 1 Drug(s) Interacting with This Target
Drug Name Drug Type External ID
Pf-04523655 siRNA drug D01VVL

References

1 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
2 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
3 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
4 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840