Details of the Target

General Information of Target

Target ID LDTP12538
Target Name Ras-related protein Rab-6B (RAB6B)
Gene Name RAB6B
Gene ID 51560
Synonyms
Ras-related protein Rab-6B; EC 3.6.5.2
3D Structure
Download
2D Sequence (FASTA)
Download
3D Structure (PDB)
Download
Sequence
MSRQAKDDFLRHYTVSDPRTHPKGYTEYKVTAQFISKKDPEDVKEVVVWKRYSDFRKLHG
DLAYTHRNLFRRLEEFPAFPRAQVFGRFEASVIEERRKGAEDLLRFTVHIPALNNSPQLK
EFFRGGEVTRPLEVSRDLHILPPPLIPTPPPDDPRLSQLLPAERRGLEELEVPVDPPPSS
PAQEALDLLFNCESTEEASGSPARGPLTEAELALFDPFSKEEGAAPSPTHVAELATMEVE
SARLDQEPWEPGGQEEEEDGEGGPTPAYLSQATELITQALRDEKAGAYAAALQGYRDGVH
VLLQGVPSDPLPARQEGVKKKAAEYLKRAEEILRLHLSQLPP
Target Bioclass
Enzyme
Family
Small GTPase superfamily, Rab family
Subcellular location
Golgi apparatus membrane
Function
The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between active GTP-bound and inactive GDP-bound states. In their active state, drive transport of vesicular carriers from donor organelles to acceptor organelles to regulate the membrane traffic that maintains organelle identity and morphology (Probable). Recruits VPS13B to the Golgi membrane. Regulates the compacted morphology of the Golgi. Seems to have a role in retrograde membrane traffic at the level of the Golgi complex. May function in retrograde transport in neuronal cells. Plays a role in neuron projection development.
Uniprot ID
Q9NRW1
Ensemble ID
ENST00000285208.9
HGNC ID
HGNC:14902

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
HT115 SNV: p.R168Ter .
LS180 SNV: p.K195N .
RKO SNV: p.G20R .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 6 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
YN-1
 Probe Info  		100.00  LDD0444  [1]
AZ-9
 Probe Info  		E58(1.06)  LDD2208  [2]
P21
 Probe Info  		6.97  LDD0408  [3]
P26
 Probe Info  		1.96  LDD0409  [3]
Jackson_14
 Probe Info  		2.86  LDD0123  [4]
HPAP
 Probe Info  		3.32  LDD0063  [5]
PAL-AfBPP Probe
Click To Hide/Show 3 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
HM-1
 Probe Info  		1.61  LDD0438  [6]
AR-1
 Probe Info  		1.55  LDD0439  [6]
AR-2
 Probe Info  		2.11  LDD0437  [6]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0174  Arenobufagin Hep-G2 2.11  LDD0437  [6]
 LDCM0173  HM30181 Hep-G2 1.61  LDD0438  [6]
 LDCM0158  P27 BxPC-3 6.97  LDD0408  [3]
 LDCM0159  P28 BxPC-3 1.96  LDD0409  [3]
 LDCM0014  Panhematin K562 3.32  LDD0063  [5]
 LDCM0016  Ranjitkar_cp1 MDA-MB-231 2.86  LDD0123  [4]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Phosphomannomutase 1 (PMM1) Eukaryotic PMM family Q92871
Other
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Conserved oligomeric Golgi complex subunit 6 (COG6) COG6 family Q9Y2V7

References

1 Ynamide Electrophile for the Profiling of Ligandable Carboxyl Residues in Live Cells and the Development of New Covalent Inhibitors. J Med Chem. 2022 Aug 11;65(15):10408-10418. doi: 10.1021/acs.jmedchem.2c00272. Epub 2022 Jul 26.
2 2H-Azirine-Based Reagents for Chemoselective Bioconjugation at Carboxyl Residues Inside Live Cells. J Am Chem Soc. 2020 Apr 1;142(13):6051-6059. doi: 10.1021/jacs.9b12116. Epub 2020 Mar 23.
3 Integrated phenotypic screening and activity-based protein profiling to reveal potential therapy targets of pancreatic cancer. Chem Commun (Camb). 2019 Jan 31;55(11):1596-1599. doi: 10.1039/c8cc08753a.
4 Appendage and Scaffold Diverse Fully Functionalized Small-Molecule Probes via a Minimalist Terminal Alkyne-Aliphatic Diazirine Isocyanide. J Org Chem. 2018 Sep 21;83(18):11245-11253. doi: 10.1021/acs.joc.8b01831. Epub 2018 Aug 31.
5 A Chemical Proteomic Map of Heme-Protein Interactions. J Am Chem Soc. 2022 Aug 24;144(33):15013-15019. doi: 10.1021/jacs.2c06104. Epub 2022 Aug 12.
Mass spectrometry data entry: PXD034651
6 Affinity-Based Protein Profiling Reveals Cellular Targets of Photoreactive Anticancer Inhibitors. ACS Chem Biol. 2019 Dec 20;14(12):2546-2552. doi: 10.1021/acschembio.9b00784. Epub 2019 Nov 26.