Details of the Target

General Information of Target

Target ID LDTP12241
Target Name Nck-associated protein 5-like (NCKAP5L)
Gene Name NCKAP5L
Gene ID 57701
Synonyms
CEP169; KIAA1602; Nck-associated protein 5-like; NCKAP5-like; Centrosomal protein of 169 kDa; Cep169
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MKSSVAQIKPSSGHDRRENLNSYQRNSSPEDRYEEQERSPRDRDYFDYSRSDYEHSRRGR
SYDSSMESRNRDREKRRERERDTDRKRSRKSPSPGRRNPETSVTQSSSAQDEPATKKKKD
ELDPLLTRTGGAYIPPAKLRMMQEQITDKNSLAYQRMSWEALKKSINGLINKVNISNISI
IIQELLQENIVRGRGLLSRSVLQAQSASPIFTHVYAALVAIINSKFPQIGELILKRLILN
FRKGYRRNDKQLCLTASKFVAHLINQNVAHEVLCLEMLTLLLERPTDDSVEVAIGFLKEC
GLKLTQVSPRGINAIFERLRNILHESEIDKRVQYMIEVMFAVRKDGFKDHPIILEGLDLV
EEDDQFTHMLPLEDDYNPEDVLNVFKMDPNFMENEEKYKAIKKEILDEGDTDSNTDQDAG
SSEEDEEEEEEEGEEDEEGQKVTIHDKTEINLVSFRRTIYLAIQSSLDFEECAHKLLKME
FPESQTKELCNMILDCCAQQRTYEKFFGLLAGRFCMLKKEYMESFEGIFKEQYDTIHRLE
TNKLRNVAKMFAHLLYTDSLPWSVLECIKLSEETTTSSSRIFVKIFFQELCEYMGLPKLN
ARLKDETLQPFFEGLLPRDNPRNTRFAINFFTSIGLGGLTDELREHLKNTPKVIVAQKPD
VEQNKSSPSSSSSASSSSESDSSDSDSDSSDSSSESSSEESDSSSISSHSSASANDVRKK
GHGKTRSKEVDKLIRNQQTNDRKQKERRQEHGHQETRTERERRSEKHRDQNSSGSNWRDP
ITKYTSDKDVPSERNNYSRVANDRDQEMHIDLENKHGDPKKKRGERRNSFSENEKHTHRI
KDSENFRRKDRSKSKEMNRKHSGSRSDEDRYQNGAERRWEKSSRYSEQSRESKKNQDRRR
EKSPAKQK
Target Bioclass
Other
Subcellular location
Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
Function
Regulates microtubule organization and stabilization. Promotes microtubule growth and bundling formation and stabilizes microtubules by increasing intense acetylation of microtubules. Both tubulin-binding and homodimer formation are required for NCKAP5L-mediated microtubule bundle formation.
Uniprot ID
Q9HCH0
Ensemble ID
ENST00000335999.7
HGNC ID
HGNC:29321

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
A3KAW SNV: p.Q517E .
CAL120 Deletion: p.R213LfsTer16 .
CCSW1 SNV: p.Q49Ter .
COLO792 SNV: p.P478S; p.A975V .
HCC15 SNV: p.D1221Y .
HCT116 SNV: p.D663G .
HS936T SNV: p.S642F .
ICC4 SNV: p.M1250L .
IGR1 SNV: p.S299F .
IGROV1 SNV: p.L328M .
JURKAT SNV: p.G1303S .
KMRC3 Deletion: p.S470AfsTer118 .
LOXIMVI SNV: p.P1091A .
MEWO Substitution: p.P819F .
MOLT4 SNV: p.M972K .
NCIH1048 Insertion: p.S606QfsTer204 .
NCIH23 SNV: p.G249C .
NUGC3 Deletion: p.Q789RfsTer46
SNV: p.G1309A		 .
RL SNV: p.D1101G .
SJSA1 SNV: p.P1294L .
SUPT1 Insertion: p.I725DfsTer85 .
TE4 SNV: p.K644N .
TGBC1TKB Deletion: p.Y1019_Q1020delinsTer .
TOV21G SNV: p.G399C .
U118MG SNV: p.A858D .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 6 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
m-APA
 Probe Info  		11.58  LDD0403  [1]
DBIA
 Probe Info  		C787(2.62)  LDD3419  [2]
AHL-Pu-1
 Probe Info  		C783(2.04)  LDD0169  [3]
IA-alkyne
 Probe Info  		N.A.  LDD0165  [4]
IPM
 Probe Info  		N.A.  LDD0147  [5]
TFBX
 Probe Info  		C787(0.00); C61(0.00)  LDD0148  [5]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0025  4SU-RNA DM93 C1210(4.37); C604(5.25)  LDD0170  [3]
 LDCM0026  4SU-RNA+native RNA HEK-293T C783(2.04)  LDD0169  [3]
 LDCM0237  AC12 HEK-293T C853(1.11)  LDD1510  [6]
 LDCM0270  AC15 HEK-293T C61(1.02); C853(0.86)  LDD1513  [6]
 LDCM0280  AC20 HEK-293T C853(1.02)  LDD1519  [6]
 LDCM0281  AC21 HEK-293T C61(1.28); C853(1.01)  LDD1520  [6]
 LDCM0283  AC23 HEK-293T C61(0.90); C853(0.63)  LDD1522  [6]
 LDCM0284  AC24 HEK-293T C61(0.95)  LDD1523  [6]
 LDCM0288  AC28 HEK-293T C853(1.29)  LDD1527  [6]
 LDCM0289  AC29 HEK-293T C61(1.17); C853(1.32)  LDD1528  [6]
 LDCM0292  AC31 HEK-293T C61(1.02); C853(0.72)  LDD1531  [6]
 LDCM0293  AC32 HEK-293T C61(1.06)  LDD1532  [6]
 LDCM0297  AC36 HEK-293T C853(1.13)  LDD1536  [6]
 LDCM0298  AC37 HEK-293T C61(1.25); C853(0.99)  LDD1537  [6]
 LDCM0300  AC39 HEK-293T C61(1.14); C853(0.88)  LDD1539  [6]
 LDCM0301  AC4 HEK-293T C853(1.19)  LDD1540  [6]
 LDCM0302  AC40 HEK-293T C61(1.15)  LDD1541  [6]
 LDCM0306  AC44 HEK-293T C853(0.92)  LDD1545  [6]
 LDCM0307  AC45 HEK-293T C61(1.05); C853(1.17)  LDD1546  [6]
 LDCM0309  AC47 HEK-293T C61(0.97); C853(0.76)  LDD1548  [6]
 LDCM0310  AC48 HEK-293T C61(1.22)  LDD1549  [6]
 LDCM0312  AC5 HEK-293T C61(1.06); C853(0.98)  LDD1551  [6]
 LDCM0315  AC52 HEK-293T C853(0.95)  LDD1554  [6]
 LDCM0316  AC53 HEK-293T C61(1.05); C853(1.30)  LDD1555  [6]
 LDCM0318  AC55 HEK-293T C61(0.97); C853(1.01)  LDD1557  [6]
 LDCM0319  AC56 HEK-293T C61(1.21)  LDD1558  [6]
 LDCM0324  AC60 HEK-293T C853(1.10)  LDD1563  [6]
 LDCM0325  AC61 HEK-293T C61(1.05); C853(1.07)  LDD1564  [6]
 LDCM0327  AC63 HEK-293T C61(1.11); C853(0.80)  LDD1566  [6]
 LDCM0328  AC64 HEK-293T C61(1.04)  LDD1567  [6]
 LDCM0334  AC7 HEK-293T C61(0.97); C853(0.85)  LDD1568  [6]
 LDCM0345  AC8 HEK-293T C61(1.01)  LDD1569  [6]
 LDCM0248  AKOS034007472 HEK-293T C61(1.23); C853(1.00)  LDD1511  [6]
 LDCM0275  AKOS034007705 HEK-293T C61(1.00)  LDD1514  [6]
 LDCM0156  Aniline NCI-H1299 11.58  LDD0403  [1]
 LDCM0369  CL100 HEK-293T C61(0.95)  LDD1573  [6]
 LDCM0371  CL102 HEK-293T C61(0.80)  LDD1575  [6]
 LDCM0373  CL104 HEK-293T C61(0.93)  LDD1577  [6]
 LDCM0375  CL106 HEK-293T C61(0.88)  LDD1579  [6]
 LDCM0377  CL108 HEK-293T C61(0.91)  LDD1581  [6]
 LDCM0379  CL11 HEK-293T C61(0.99); C853(1.18)  LDD1583  [6]
 LDCM0380  CL110 HEK-293T C61(0.89)  LDD1584  [6]
 LDCM0382  CL112 HEK-293T C61(0.88)  LDD1586  [6]
 LDCM0384  CL114 HEK-293T C61(0.88)  LDD1588  [6]
 LDCM0386  CL116 HEK-293T C61(0.94)  LDD1590  [6]
 LDCM0388  CL118 HEK-293T C61(0.97)  LDD1592  [6]
 LDCM0390  CL12 HEK-293T C61(1.16)  LDD1594  [6]
 LDCM0391  CL120 HEK-293T C61(1.00)  LDD1595  [6]
 LDCM0393  CL122 HEK-293T C61(0.90)  LDD1597  [6]
 LDCM0395  CL124 HEK-293T C61(0.96)  LDD1599  [6]
 LDCM0397  CL126 HEK-293T C61(0.96)  LDD1601  [6]
 LDCM0399  CL128 HEK-293T C61(0.95)  LDD1603  [6]
 LDCM0401  CL14 HEK-293T C61(0.96)  LDD1605  [6]
 LDCM0403  CL16 HEK-293T C61(1.07)  LDD1607  [6]
 LDCM0407  CL2 HEK-293T C61(0.95)  LDD1611  [6]
 LDCM0408  CL20 HEK-293T C853(1.08)  LDD1612  [6]
 LDCM0409  CL21 HEK-293T C61(0.27); C853(0.92)  LDD1613  [6]
 LDCM0411  CL23 HEK-293T C61(1.01); C853(0.91)  LDD1615  [6]
 LDCM0412  CL24 HEK-293T C61(1.22)  LDD1616  [6]
 LDCM0414  CL26 HEK-293T C61(1.03)  LDD1618  [6]
 LDCM0416  CL28 HEK-293T C61(0.93)  LDD1620  [6]
 LDCM0421  CL32 HEK-293T C853(1.20)  LDD1625  [6]
 LDCM0422  CL33 HEK-293T C61(1.04); C853(1.17)  LDD1626  [6]
 LDCM0424  CL35 HEK-293T C61(0.90); C853(1.06)  LDD1628  [6]
 LDCM0425  CL36 HEK-293T C61(0.95)  LDD1629  [6]
 LDCM0429  CL4 HEK-293T C61(0.89)  LDD1633  [6]
 LDCM0430  CL40 HEK-293T C61(0.92)  LDD1634  [6]
 LDCM0434  CL44 HEK-293T C853(1.11)  LDD1638  [6]
 LDCM0435  CL45 HEK-293T C61(0.97); C853(0.87)  LDD1639  [6]
 LDCM0437  CL47 HEK-293T C61(0.92); C853(0.82)  LDD1641  [6]
 LDCM0438  CL48 HEK-293T C61(1.03)  LDD1642  [6]
 LDCM0441  CL50 HEK-293T C61(0.94)  LDD1645  [6]
 LDCM0443  CL52 HEK-293T C61(0.96)  LDD1646  [6]
 LDCM0447  CL56 HEK-293T C853(0.85)  LDD1650  [6]
 LDCM0448  CL57 HEK-293T C61(1.20); C853(1.23)  LDD1651  [6]
 LDCM0450  CL59 HEK-293T C61(0.95); C853(0.78)  LDD1653  [6]
 LDCM0452  CL60 HEK-293T C61(1.14)  LDD1655  [6]
 LDCM0454  CL62 HEK-293T C61(1.04)  LDD1657  [6]
 LDCM0456  CL64 HEK-293T C61(1.02)  LDD1659  [6]
 LDCM0460  CL68 HEK-293T C853(1.26)  LDD1663  [6]
 LDCM0461  CL69 HEK-293T C61(1.15); C853(1.03)  LDD1664  [6]
 LDCM0464  CL71 HEK-293T C61(0.97); C853(1.19)  LDD1667  [6]
 LDCM0465  CL72 HEK-293T C61(1.08)  LDD1668  [6]
 LDCM0467  CL74 HEK-293T C61(0.91)  LDD1670  [6]
 LDCM0469  CL76 HEK-293T C61(0.94)  LDD1672  [6]
 LDCM0473  CL8 HEK-293T C853(1.19)  LDD1676  [6]
 LDCM0474  CL80 HEK-293T C853(1.03)  LDD1677  [6]
 LDCM0475  CL81 HEK-293T C61(1.03); C853(1.24)  LDD1678  [6]
 LDCM0477  CL83 HEK-293T C61(0.87); C853(0.73)  LDD1680  [6]
 LDCM0478  CL84 HEK-293T C61(1.03)  LDD1681  [6]
 LDCM0480  CL86 HEK-293T C61(1.04)  LDD1683  [6]
 LDCM0482  CL88 HEK-293T C61(1.09)  LDD1685  [6]
 LDCM0484  CL9 HEK-293T C61(1.18); C853(1.02)  LDD1687  [6]
 LDCM0487  CL92 HEK-293T C853(0.99)  LDD1690  [6]
 LDCM0488  CL93 HEK-293T C61(1.14); C853(0.88)  LDD1691  [6]
 LDCM0490  CL95 HEK-293T C61(0.98); C853(1.08)  LDD1693  [6]
 LDCM0491  CL96 HEK-293T C61(1.03)  LDD1694  [6]
 LDCM0493  CL98 HEK-293T C61(0.96)  LDD1696  [6]
 LDCM0427  Fragment51 HEK-293T C61(1.04)  LDD1631  [6]
 LDCM0022  KB02 G-361 C787(2.79)  LDD2329  [2]
 LDCM0023  KB03 G-361 C787(5.58)  LDD2746  [2]
 LDCM0024  KB05 SH-4 C787(2.62)  LDD3419  [2]

References

1 Quantitative and Site-Specific Chemoproteomic Profiling of Targets of Acrolein. Chem Res Toxicol. 2019 Mar 18;32(3):467-473. doi: 10.1021/acs.chemrestox.8b00343. Epub 2019 Jan 15.
2 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
3 Chemoproteomic capture of RNA binding activity in living cells. Nat Commun. 2023 Oct 7;14(1):6282. doi: 10.1038/s41467-023-41844-z.
Mass spectrometry data entry: PXD044625
4 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
5 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
6 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402