Details of the Target

General Information of Target

Target ID LDTP11137
Target Name Suppressor of IKBKE 1 (SIKE1)
Gene Name SIKE1
Gene ID 80143
Synonyms
SIKE; Suppressor of IKBKE 1; Suppressor of IKK-epsilon
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MEAGGFLDSLIYGACVVFTLGMFSAGLSDLRHMRMTRSVDNVQFLPFLTTEVNNLGWLSY
GALKGDGILIVVNTVGAALQTLYILAYLHYCPRKRVVLLQTATLLGVLLLGYGYFWLLVP
NPEARLQQLGLFCSVFTISMYLSPLADLAKVIQTKSTQCLSYPLTIATLLTSASWCLYGF
RLRDPYIMVSNFPGIVTSFIRFWLFWKYPQEQDRNYWLLQT
Target Bioclass
Other
Family
SIKE family
Subcellular location
Cytoplasm
Function
Physiological suppressor of IKK-epsilon and TBK1 that plays an inhibitory role in virus- and TLR3-triggered IRF3. Inhibits TLR3-mediated activation of interferon-stimulated response elements (ISRE) and the IFN-beta promoter. May act by disrupting the interactions of IKBKE or TBK1 with TICAM1/TRIF, IRF3 and RIGI. Does not inhibit NF-kappa-B activation pathways.
Uniprot ID
Q9BRV8
Ensemble ID
ENST00000060969.6
HGNC ID
HGNC:26119

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
HLF Insertion: p.S133_A136dup .
HS936T SNV: p.S190F .
HUCCT1 SNV: p.T48A .
JURKAT SNV: p.Q79E .
KYM1 Insertion: p.L115_A136dup .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 9 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
m-APA
 Probe Info  		15.00  LDD0402  [1]
TG42
 Probe Info  		11.58  LDD0043  [2]
Probe 1
 Probe Info  		Y54(37.02)  LDD3495  [3]
DBIA
 Probe Info  		C150(4.22)  LDD3367  [4]
IA-alkyne
 Probe Info  		N.A.  LDD2241  [5]
NAIA_5
 Probe Info  		N.A.  LDD2224  [6]
IPM
 Probe Info  		N.A.  LDD2156  [7]
VSF
 Probe Info  		N.A.  LDD0007  [8]
AOyne
 Probe Info  		15.00  LDD0443  [9]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0214  AC1 HEK-293T C146(1.27)  LDD1507  [10]
 LDCM0215  AC10 HEK-293T C146(0.98)  LDD1508  [10]
 LDCM0226  AC11 HEK-293T C146(1.08)  LDD1509  [10]
 LDCM0237  AC12 HEK-293T C146(1.31)  LDD1510  [10]
 LDCM0259  AC14 HEK-293T C146(1.27)  LDD1512  [10]
 LDCM0270  AC15 HEK-293T C146(1.21)  LDD1513  [10]
 LDCM0276  AC17 HEK-293T C146(1.39)  LDD1515  [10]
 LDCM0277  AC18 HEK-293T C146(0.41)  LDD1516  [10]
 LDCM0278  AC19 HEK-293T C146(1.00)  LDD1517  [10]
 LDCM0279  AC2 HEK-293T C146(0.98)  LDD1518  [10]
 LDCM0280  AC20 HEK-293T C146(1.21)  LDD1519  [10]
 LDCM0281  AC21 HEK-293T C146(1.23)  LDD1520  [10]
 LDCM0282  AC22 HEK-293T C146(1.07)  LDD1521  [10]
 LDCM0283  AC23 HEK-293T C146(1.28)  LDD1522  [10]
 LDCM0284  AC24 HEK-293T C146(1.07)  LDD1523  [10]
 LDCM0285  AC25 HEK-293T C146(1.45)  LDD1524  [10]
 LDCM0286  AC26 HEK-293T C146(1.00)  LDD1525  [10]
 LDCM0287  AC27 HEK-293T C146(0.89)  LDD1526  [10]
 LDCM0288  AC28 HEK-293T C146(1.30)  LDD1527  [10]
 LDCM0289  AC29 HEK-293T C146(0.96)  LDD1528  [10]
 LDCM0290  AC3 HEK-293T C146(0.87)  LDD1529  [10]
 LDCM0291  AC30 HEK-293T C146(1.06)  LDD1530  [10]
 LDCM0292  AC31 HEK-293T C146(1.05)  LDD1531  [10]
 LDCM0293  AC32 HEK-293T C146(1.33)  LDD1532  [10]
 LDCM0294  AC33 HEK-293T C146(1.43)  LDD1533  [10]
 LDCM0295  AC34 HEK-293T C146(0.99)  LDD1534  [10]
 LDCM0296  AC35 HEK-293T C146(1.22)  LDD1535  [10]
 LDCM0297  AC36 HEK-293T C146(1.63)  LDD1536  [10]
 LDCM0298  AC37 HEK-293T C146(1.14)  LDD1537  [10]
 LDCM0299  AC38 HEK-293T C146(1.25)  LDD1538  [10]
 LDCM0300  AC39 HEK-293T C146(1.35)  LDD1539  [10]
 LDCM0301  AC4 HEK-293T C146(1.05)  LDD1540  [10]
 LDCM0302  AC40 HEK-293T C146(1.12)  LDD1541  [10]
 LDCM0303  AC41 HEK-293T C146(1.24)  LDD1542  [10]
 LDCM0304  AC42 HEK-293T C146(1.01)  LDD1543  [10]
 LDCM0305  AC43 HEK-293T C146(1.17)  LDD1544  [10]
 LDCM0306  AC44 HEK-293T C146(1.61)  LDD1545  [10]
 LDCM0307  AC45 HEK-293T C146(1.07)  LDD1546  [10]
 LDCM0308  AC46 HEK-293T C146(1.13)  LDD1547  [10]
 LDCM0309  AC47 HEK-293T C146(1.13)  LDD1548  [10]
 LDCM0310  AC48 HEK-293T C146(1.67)  LDD1549  [10]
 LDCM0311  AC49 HEK-293T C146(1.14)  LDD1550  [10]
 LDCM0312  AC5 HEK-293T C146(1.09)  LDD1551  [10]
 LDCM0313  AC50 HEK-293T C146(1.18)  LDD1552  [10]
 LDCM0314  AC51 HEK-293T C146(1.42)  LDD1553  [10]
 LDCM0315  AC52 HEK-293T C146(1.11)  LDD1554  [10]
 LDCM0316  AC53 HEK-293T C146(1.31)  LDD1555  [10]
 LDCM0317  AC54 HEK-293T C146(1.02)  LDD1556  [10]
 LDCM0318  AC55 HEK-293T C146(1.08)  LDD1557  [10]
 LDCM0319  AC56 HEK-293T C146(1.06)  LDD1558  [10]
 LDCM0320  AC57 HEK-293T C146(1.42)  LDD1559  [10]
 LDCM0321  AC58 HEK-293T C146(0.94)  LDD1560  [10]
 LDCM0322  AC59 HEK-293T C146(1.14)  LDD1561  [10]
 LDCM0323  AC6 HEK-293T C146(1.08)  LDD1562  [10]
 LDCM0324  AC60 HEK-293T C146(1.23)  LDD1563  [10]
 LDCM0325  AC61 HEK-293T C146(1.13)  LDD1564  [10]
 LDCM0326  AC62 HEK-293T C146(1.14)  LDD1565  [10]
 LDCM0327  AC63 HEK-293T C146(1.20)  LDD1566  [10]
 LDCM0328  AC64 HEK-293T C146(0.95)  LDD1567  [10]
 LDCM0334  AC7 HEK-293T C146(0.78)  LDD1568  [10]
 LDCM0345  AC8 HEK-293T C146(0.71)  LDD1569  [10]
 LDCM0248  AKOS034007472 HEK-293T C146(1.41)  LDD1511  [10]
 LDCM0356  AKOS034007680 HEK-293T C146(1.05)  LDD1570  [10]
 LDCM0275  AKOS034007705 HEK-293T C146(1.20)  LDD1514  [10]
 LDCM0156  Aniline NCI-H1299 11.92  LDD0403  [1]
 LDCM0368  CL10 HEK-293T C146(0.62)  LDD1572  [10]
 LDCM0372  CL103 HEK-293T C146(1.16)  LDD1576  [10]
 LDCM0376  CL107 HEK-293T C146(1.43)  LDD1580  [10]
 LDCM0379  CL11 HEK-293T C146(0.87)  LDD1583  [10]
 LDCM0381  CL111 HEK-293T C146(0.93)  LDD1585  [10]
 LDCM0385  CL115 HEK-293T C146(1.25)  LDD1589  [10]
 LDCM0389  CL119 HEK-293T C146(1.14)  LDD1593  [10]
 LDCM0390  CL12 HEK-293T C146(1.04)  LDD1594  [10]
 LDCM0394  CL123 HEK-293T C146(1.13)  LDD1598  [10]
 LDCM0398  CL127 HEK-293T C146(1.39)  LDD1602  [10]
 LDCM0402  CL15 HEK-293T C146(1.24)  LDD1606  [10]
 LDCM0404  CL17 HEK-293T C146(1.22)  LDD1608  [10]
 LDCM0405  CL18 HEK-293T C146(1.17)  LDD1609  [10]
 LDCM0406  CL19 HEK-293T C146(1.04)  LDD1610  [10]
 LDCM0408  CL20 HEK-293T C146(1.25)  LDD1612  [10]
 LDCM0409  CL21 HEK-293T C146(1.30)  LDD1613  [10]
 LDCM0410  CL22 HEK-293T C146(1.00)  LDD1614  [10]
 LDCM0411  CL23 HEK-293T C146(1.12)  LDD1615  [10]
 LDCM0412  CL24 HEK-293T C146(0.99)  LDD1616  [10]
 LDCM0415  CL27 HEK-293T C146(1.17)  LDD1619  [10]
 LDCM0417  CL29 HEK-293T C146(1.44)  LDD1621  [10]
 LDCM0418  CL3 HEK-293T C146(0.88)  LDD1622  [10]
 LDCM0419  CL30 HEK-293T C146(1.03)  LDD1623  [10]
 LDCM0420  CL31 HEK-293T C146(0.87)  LDD1624  [10]
 LDCM0421  CL32 HEK-293T C146(1.31)  LDD1625  [10]
 LDCM0422  CL33 HEK-293T C146(1.30)  LDD1626  [10]
 LDCM0423  CL34 HEK-293T C146(0.81)  LDD1627  [10]
 LDCM0424  CL35 HEK-293T C146(1.42)  LDD1628  [10]
 LDCM0425  CL36 HEK-293T C146(1.37)  LDD1629  [10]
 LDCM0428  CL39 HEK-293T C146(1.22)  LDD1632  [10]
 LDCM0431  CL41 HEK-293T C146(1.31)  LDD1635  [10]
 LDCM0432  CL42 HEK-293T C146(1.04)  LDD1636  [10]
 LDCM0433  CL43 HEK-293T C146(1.05)  LDD1637  [10]
 LDCM0434  CL44 HEK-293T C146(1.30)  LDD1638  [10]
 LDCM0435  CL45 HEK-293T C146(1.59)  LDD1639  [10]
 LDCM0436  CL46 HEK-293T C146(1.26)  LDD1640  [10]
 LDCM0437  CL47 HEK-293T C146(1.00)  LDD1641  [10]
 LDCM0438  CL48 HEK-293T C146(1.31)  LDD1642  [10]
 LDCM0440  CL5 HEK-293T C146(1.35)  LDD1644  [10]
 LDCM0444  CL53 HEK-293T C146(1.30)  LDD1647  [10]
 LDCM0445  CL54 HEK-293T C146(1.46)  LDD1648  [10]
 LDCM0446  CL55 HEK-293T C146(1.31)  LDD1649  [10]
 LDCM0447  CL56 HEK-293T C146(1.22)  LDD1650  [10]
 LDCM0448  CL57 HEK-293T C146(1.40)  LDD1651  [10]
 LDCM0449  CL58 HEK-293T C146(0.90)  LDD1652  [10]
 LDCM0450  CL59 HEK-293T C146(1.27)  LDD1653  [10]
 LDCM0451  CL6 HEK-293T C146(1.21)  LDD1654  [10]
 LDCM0452  CL60 HEK-293T C146(1.50)  LDD1655  [10]
 LDCM0455  CL63 HEK-293T C146(1.10)  LDD1658  [10]
 LDCM0457  CL65 HEK-293T C146(1.54)  LDD1660  [10]
 LDCM0458  CL66 HEK-293T C146(1.21)  LDD1661  [10]
 LDCM0459  CL67 HEK-293T C146(0.94)  LDD1662  [10]
 LDCM0460  CL68 HEK-293T C146(1.23)  LDD1663  [10]
 LDCM0461  CL69 HEK-293T C146(1.31)  LDD1664  [10]
 LDCM0462  CL7 HEK-293T C146(1.13)  LDD1665  [10]
 LDCM0463  CL70 HEK-293T C146(0.93)  LDD1666  [10]
 LDCM0464  CL71 HEK-293T C146(1.25)  LDD1667  [10]
 LDCM0465  CL72 HEK-293T C146(1.51)  LDD1668  [10]
 LDCM0470  CL77 HEK-293T C146(0.99)  LDD1673  [10]
 LDCM0471  CL78 HEK-293T C146(1.05)  LDD1674  [10]
 LDCM0472  CL79 HEK-293T C146(0.98)  LDD1675  [10]
 LDCM0473  CL8 HEK-293T C146(0.72)  LDD1676  [10]
 LDCM0474  CL80 HEK-293T C146(0.87)  LDD1677  [10]
 LDCM0475  CL81 HEK-293T C146(1.11)  LDD1678  [10]
 LDCM0476  CL82 HEK-293T C146(0.94)  LDD1679  [10]
 LDCM0477  CL83 HEK-293T C146(0.90)  LDD1680  [10]
 LDCM0478  CL84 HEK-293T C146(0.94)  LDD1681  [10]
 LDCM0481  CL87 HEK-293T C146(1.31)  LDD1684  [10]
 LDCM0483  CL89 HEK-293T C146(1.29)  LDD1686  [10]
 LDCM0484  CL9 HEK-293T C146(0.75)  LDD1687  [10]
 LDCM0485  CL90 HEK-293T C146(1.32)  LDD1688  [10]
 LDCM0486  CL91 HEK-293T C146(1.14)  LDD1689  [10]
 LDCM0487  CL92 HEK-293T C146(1.23)  LDD1690  [10]
 LDCM0488  CL93 HEK-293T C146(1.29)  LDD1691  [10]
 LDCM0489  CL94 HEK-293T C146(1.07)  LDD1692  [10]
 LDCM0490  CL95 HEK-293T C146(1.11)  LDD1693  [10]
 LDCM0491  CL96 HEK-293T C146(1.05)  LDD1694  [10]
 LDCM0494  CL99 HEK-293T C146(0.91)  LDD1697  [10]
 LDCM0634  CY-0357 Hep-G2 C164(2.56); C164(1.30)  LDD2228  [6]
 LDCM0495  E2913 HEK-293T C146(1.26)  LDD1698  [10]
 LDCM0468  Fragment33 HEK-293T C146(1.06)  LDD1671  [10]
 LDCM0022  KB02 HEK-293T C164(1.03)  LDD1492  [10]
 LDCM0023  KB03 HEK-293T C164(1.04)  LDD1497  [10]
 LDCM0024  KB05 NUGC-3 C150(4.22)  LDD3367  [4]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Protein N-terminal glutamine amidohydrolase (NTAQ1) NTAQ1 family Q96HA8
Other
Click To Hide/Show 6 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Centromere protein Q (CENPQ) CENP-Q/OKP1 family Q7L2Z9
Cancer/testis antigen 1 (CTAG1A; CTAG1B) CTAG/PCC1 family P78358
Syntaxin-11 (STX11) Syntaxin family O75558
Alpha-taxilin (TXLNA) Taxilin family P40222
Beta-taxilin (TXLNB) Taxilin family Q8N3L3
TRAF3-interacting JNK-activating modulator (TRAF3IP3) . Q9Y228

References

1 Quantitative and Site-Specific Chemoproteomic Profiling of Targets of Acrolein. Chem Res Toxicol. 2019 Mar 18;32(3):467-473. doi: 10.1021/acs.chemrestox.8b00343. Epub 2019 Jan 15.
2 Design and synthesis of tailored human caseinolytic protease P inhibitors. Chem Commun (Camb). 2018 Aug 28;54(70):9833-9836. doi: 10.1039/c8cc05265d.
Mass spectrometry data entry: PXD010277
3 An Azo Coupling-Based Chemoproteomic Approach to Systematically Profile the Tyrosine Reactivity in the Human Proteome. Anal Chem. 2021 Jul 27;93(29):10334-10342. doi: 10.1021/acs.analchem.1c01935. Epub 2021 Jul 12.
4 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
5 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
6 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
7 Benchmarking Cleavable Biotin Tags for Peptide-Centric Chemoproteomics. J Proteome Res. 2022 May 6;21(5):1349-1358. doi: 10.1021/acs.jproteome.2c00174. Epub 2022 Apr 25.
Mass spectrometry data entry: PXD031019
8 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
9 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.
10 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402