Details of the Target

General Information of Target

Target ID LDTP10075
Target Name Protein N-terminal asparagine amidohydrolase (NTAN1)
Gene Name NTAN1
Gene ID 123803
Synonyms
Protein N-terminal asparagine amidohydrolase; EC 3.5.1.121; Protein NH2-terminal asparagine amidohydrolase; PNAA; Protein NH2-terminal asparagine deamidase; PNAD; Protein N-terminal Asn amidase; Protein N-terminal asparagine amidase; Protein NTN-amidase
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MSKKGRNKGEKPEALIVALQAANEDLRTKLTDIQIELHQEKSKVSKLEREKTQEAKRIRE
LEQRKHTVLVTELKAKLHEEKMKELQAVRENLIKQHEQEMSRTVKVRDGEIQRLKSALCA
LRDGSSDKVRTALTIEAREEARKLFDTERLKLLQEIADLKTAKKQVDEALSNMIQADKIK
AGDLRSEHQSHQEAISKIKWESERDIRRLMDEIKAKDRIIFSLEKELETQTGYVQKLQLQ
KEALDEQLFLVKEAECNMSSPKREIPGRAGDGSEHCSSPDLRRNQKRIAELNATIRKLED
RNTLLGDERNELLKRVRETEKQCKPLLERNKCLAKRNDELMVSLQRMEEKLKAVTKENSE
MREKITSHPPLKKLKSLNDLDQANEEQETEFLKLQVIEQQNIIDELTRDREKLIRRRKHR
RSSKPIKRPVLDPFIGYDEDSMDSETSSMASFRTDRTPATPDDDLDESLAAEESELRFRQ
LTKEYQALQRAYALLQEQTGGIIDAEREAKAQEQLQAEVLRYKAKIEDLEATLAQKGQDS
HWVEDKQLFIKRNQELLEKIEKQEAENHRLQQELQDARDQNELLEFRNLELEERERRSPP
FNLQIHPFSDGVSALQIYCMKEGVKDVNIPDLIKQLDILGDNGNLRNEEQVAIIQASTVL
SLAEKWIQQIEGAEAALHQKMMELESDMEQFCKIKGYLEEELDYRKQALDQAYMRIQELE
ATLYNALQQETVIKFGELLSEKQQEELRTAVEKLRRQMLRKSREYDCQILQERMELLQQA
HQRIRDLEDKTDIQKRQIKDLEEKSNRKHG
Target Bioclass
Enzyme
Subcellular location
Cytoplasm
Function
N-terminal asparagine deamidase that mediates deamidation of N-terminal asparagine residues to aspartate. Required for the ubiquitin-dependent turnover of intracellular proteins that initiate with Met-Asn. These proteins are acetylated on the retained initiator methionine and can subsequently be modified by the removal of N-acetyl methionine by acylaminoacid hydrolase (AAH). Conversion of the resulting N-terminal asparagine to aspartate by NTAN1/PNAD renders the protein susceptible to arginylation, polyubiquitination and degradation as specified by the N-end rule. This enzyme does not act on substrates with internal or C-terminal asparagines and does not act on glutamine residues in any position, nor on acetylated N-terminal peptidyl Asn.
Uniprot ID
Q96AB6
Ensemble ID
ENST00000287706.8
HGNC ID
HGNC:29909

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 5 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C118(1.45)  LDD3335  [1]
BTD
 Probe Info  		C118(0.72)  LDD2091  [2]
IPM
 Probe Info  		C118(0.91)  LDD1702  [2]
IA-alkyne
 Probe Info  		N.A.  LDD0036  [3]
Lodoacetamide azide
 Probe Info  		N.A.  LDD0037  [3]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0026  4SU-RNA+native RNA HEK-293T C118(2.08)  LDD0372  [4]
 LDCM0215  AC10 HEK-293T C118(1.02)  LDD1508  [5]
 LDCM0226  AC11 HEK-293T C118(0.98)  LDD1509  [5]
 LDCM0259  AC14 HEK-293T C118(1.08)  LDD1512  [5]
 LDCM0270  AC15 HEK-293T C118(0.97)  LDD1513  [5]
 LDCM0277  AC18 HEK-293T C118(0.95)  LDD1516  [5]
 LDCM0278  AC19 HEK-293T C118(1.02)  LDD1517  [5]
 LDCM0279  AC2 HEK-293T C118(1.00)  LDD1518  [5]
 LDCM0281  AC21 HEK-293T C118(0.95)  LDD1520  [5]
 LDCM0282  AC22 HEK-293T C118(1.04)  LDD1521  [5]
 LDCM0283  AC23 HEK-293T C118(1.01)  LDD1522  [5]
 LDCM0284  AC24 HEK-293T C118(1.01)  LDD1523  [5]
 LDCM0286  AC26 HEK-293T C118(0.91)  LDD1525  [5]
 LDCM0287  AC27 HEK-293T C118(1.02)  LDD1526  [5]
 LDCM0289  AC29 HEK-293T C118(1.02)  LDD1528  [5]
 LDCM0290  AC3 HEK-293T C118(0.96)  LDD1529  [5]
 LDCM0291  AC30 HEK-293T C118(0.90)  LDD1530  [5]
 LDCM0292  AC31 HEK-293T C118(1.04)  LDD1531  [5]
 LDCM0293  AC32 HEK-293T C118(1.11)  LDD1532  [5]
 LDCM0295  AC34 HEK-293T C118(1.13)  LDD1534  [5]
 LDCM0296  AC35 HEK-293T C118(0.98)  LDD1535  [5]
 LDCM0298  AC37 HEK-293T C118(0.98)  LDD1537  [5]
 LDCM0299  AC38 HEK-293T C118(0.99)  LDD1538  [5]
 LDCM0300  AC39 HEK-293T C118(1.05)  LDD1539  [5]
 LDCM0302  AC40 HEK-293T C118(1.06)  LDD1541  [5]
 LDCM0304  AC42 HEK-293T C118(1.08)  LDD1543  [5]
 LDCM0305  AC43 HEK-293T C118(0.99)  LDD1544  [5]
 LDCM0307  AC45 HEK-293T C118(1.08)  LDD1546  [5]
 LDCM0308  AC46 HEK-293T C118(1.09)  LDD1547  [5]
 LDCM0309  AC47 HEK-293T C118(1.13)  LDD1548  [5]
 LDCM0310  AC48 HEK-293T C118(0.98)  LDD1549  [5]
 LDCM0312  AC5 HEK-293T C118(1.05)  LDD1551  [5]
 LDCM0313  AC50 HEK-293T C118(1.07)  LDD1552  [5]
 LDCM0314  AC51 HEK-293T C118(1.01)  LDD1553  [5]
 LDCM0316  AC53 HEK-293T C118(1.00)  LDD1555  [5]
 LDCM0317  AC54 HEK-293T C118(0.97)  LDD1556  [5]
 LDCM0318  AC55 HEK-293T C118(1.00)  LDD1557  [5]
 LDCM0319  AC56 HEK-293T C118(1.01)  LDD1558  [5]
 LDCM0321  AC58 HEK-293T C118(0.96)  LDD1560  [5]
 LDCM0322  AC59 HEK-293T C118(0.96)  LDD1561  [5]
 LDCM0323  AC6 HEK-293T C118(1.00)  LDD1562  [5]
 LDCM0325  AC61 HEK-293T C118(0.92)  LDD1564  [5]
 LDCM0326  AC62 HEK-293T C118(0.94)  LDD1565  [5]
 LDCM0327  AC63 HEK-293T C118(0.96)  LDD1566  [5]
 LDCM0328  AC64 HEK-293T C118(0.98)  LDD1567  [5]
 LDCM0334  AC7 HEK-293T C118(1.01)  LDD1568  [5]
 LDCM0345  AC8 HEK-293T C118(0.96)  LDD1569  [5]
 LDCM0248  AKOS034007472 HEK-293T C118(0.98)  LDD1511  [5]
 LDCM0275  AKOS034007705 HEK-293T C118(1.00)  LDD1514  [5]
 LDCM0498  BS-3668 MDA-MB-231 C118(0.72)  LDD2091  [2]
 LDCM0367  CL1 HEK-293T C118(1.05)  LDD1571  [5]
 LDCM0368  CL10 HEK-293T C118(1.06)  LDD1572  [5]
 LDCM0370  CL101 HEK-293T C118(0.95)  LDD1574  [5]
 LDCM0374  CL105 HEK-293T C118(0.92)  LDD1578  [5]
 LDCM0378  CL109 HEK-293T C118(1.07)  LDD1582  [5]
 LDCM0379  CL11 HEK-293T C118(1.18)  LDD1583  [5]
 LDCM0383  CL113 HEK-293T C118(1.06)  LDD1587  [5]
 LDCM0387  CL117 HEK-293T C118(0.96)  LDD1591  [5]
 LDCM0390  CL12 HEK-293T C118(1.01)  LDD1594  [5]
 LDCM0392  CL121 HEK-293T C118(0.94)  LDD1596  [5]
 LDCM0396  CL125 HEK-293T C118(0.77)  LDD1600  [5]
 LDCM0400  CL13 HEK-293T C118(0.96)  LDD1604  [5]
 LDCM0405  CL18 HEK-293T C118(1.05)  LDD1609  [5]
 LDCM0406  CL19 HEK-293T C118(1.07)  LDD1610  [5]
 LDCM0409  CL21 HEK-293T C118(1.22)  LDD1613  [5]
 LDCM0410  CL22 HEK-293T C118(1.16)  LDD1614  [5]
 LDCM0411  CL23 HEK-293T C118(1.08)  LDD1615  [5]
 LDCM0412  CL24 HEK-293T C118(1.07)  LDD1616  [5]
 LDCM0413  CL25 HEK-293T C118(0.87)  LDD1617  [5]
 LDCM0419  CL30 HEK-293T C118(1.00)  LDD1623  [5]
 LDCM0420  CL31 HEK-293T C118(0.96)  LDD1624  [5]
 LDCM0422  CL33 HEK-293T C118(1.02)  LDD1626  [5]
 LDCM0423  CL34 HEK-293T C118(1.00)  LDD1627  [5]
 LDCM0424  CL35 HEK-293T C118(1.20)  LDD1628  [5]
 LDCM0425  CL36 HEK-293T C118(1.00)  LDD1629  [5]
 LDCM0426  CL37 HEK-293T C118(0.86)  LDD1630  [5]
 LDCM0432  CL42 HEK-293T C118(1.09)  LDD1636  [5]
 LDCM0433  CL43 HEK-293T C118(1.01)  LDD1637  [5]
 LDCM0435  CL45 HEK-293T C118(1.30)  LDD1639  [5]
 LDCM0436  CL46 HEK-293T C118(1.04)  LDD1640  [5]
 LDCM0437  CL47 HEK-293T C118(1.27)  LDD1641  [5]
 LDCM0438  CL48 HEK-293T C118(0.99)  LDD1642  [5]
 LDCM0439  CL49 HEK-293T C118(0.84)  LDD1643  [5]
 LDCM0445  CL54 HEK-293T C118(0.91)  LDD1648  [5]
 LDCM0446  CL55 HEK-293T C118(1.07)  LDD1649  [5]
 LDCM0448  CL57 HEK-293T C118(1.23)  LDD1651  [5]
 LDCM0449  CL58 HEK-293T C118(1.05)  LDD1652  [5]
 LDCM0450  CL59 HEK-293T C118(1.17)  LDD1653  [5]
 LDCM0451  CL6 HEK-293T C118(1.05)  LDD1654  [5]
 LDCM0452  CL60 HEK-293T C118(1.11)  LDD1655  [5]
 LDCM0453  CL61 HEK-293T C118(0.87)  LDD1656  [5]
 LDCM0458  CL66 HEK-293T C118(0.99)  LDD1661  [5]
 LDCM0459  CL67 HEK-293T C118(1.04)  LDD1662  [5]
 LDCM0461  CL69 HEK-293T C118(1.13)  LDD1664  [5]
 LDCM0462  CL7 HEK-293T C118(0.99)  LDD1665  [5]
 LDCM0463  CL70 HEK-293T C118(1.10)  LDD1666  [5]
 LDCM0464  CL71 HEK-293T C118(1.16)  LDD1667  [5]
 LDCM0465  CL72 HEK-293T C118(1.02)  LDD1668  [5]
 LDCM0466  CL73 HEK-293T C118(0.91)  LDD1669  [5]
 LDCM0471  CL78 HEK-293T C118(1.06)  LDD1674  [5]
 LDCM0472  CL79 HEK-293T C118(1.01)  LDD1675  [5]
 LDCM0475  CL81 HEK-293T C118(1.03)  LDD1678  [5]
 LDCM0476  CL82 HEK-293T C118(1.03)  LDD1679  [5]
 LDCM0477  CL83 HEK-293T C118(1.07)  LDD1680  [5]
 LDCM0478  CL84 HEK-293T C118(0.93)  LDD1681  [5]
 LDCM0479  CL85 HEK-293T C118(0.75)  LDD1682  [5]
 LDCM0484  CL9 HEK-293T C118(1.19)  LDD1687  [5]
 LDCM0485  CL90 HEK-293T C118(0.93)  LDD1688  [5]
 LDCM0486  CL91 HEK-293T C118(0.88)  LDD1689  [5]
 LDCM0488  CL93 HEK-293T C118(1.08)  LDD1691  [5]
 LDCM0489  CL94 HEK-293T C118(1.10)  LDD1692  [5]
 LDCM0490  CL95 HEK-293T C118(0.94)  LDD1693  [5]
 LDCM0491  CL96 HEK-293T C118(0.84)  LDD1694  [5]
 LDCM0492  CL97 HEK-293T C118(0.97)  LDD1695  [5]
 LDCM0213  Electrophilic fragment 2 MDA-MB-231 C118(0.91)  LDD1702  [2]
 LDCM0625  F8 Ramos C118(1.12)  LDD2187  [6]
 LDCM0572  Fragment10 Ramos C118(1.68)  LDD2189  [6]
 LDCM0573  Fragment11 Ramos C118(0.20)  LDD2190  [6]
 LDCM0574  Fragment12 Ramos C118(0.75)  LDD2191  [6]
 LDCM0575  Fragment13 Ramos C118(0.86)  LDD2192  [6]
 LDCM0576  Fragment14 Ramos C118(0.89)  LDD2193  [6]
 LDCM0579  Fragment20 Ramos C118(1.05)  LDD2194  [6]
 LDCM0580  Fragment21 Ramos C118(0.98)  LDD2195  [6]
 LDCM0582  Fragment23 Ramos C118(1.02)  LDD2196  [6]
 LDCM0578  Fragment27 Ramos C118(0.71)  LDD2197  [6]
 LDCM0586  Fragment28 Ramos C118(0.82)  LDD2198  [6]
 LDCM0588  Fragment30 Ramos C118(1.13)  LDD2199  [6]
 LDCM0589  Fragment31 Ramos C118(0.87)  LDD2200  [6]
 LDCM0590  Fragment32 Ramos C118(0.80)  LDD2201  [6]
 LDCM0468  Fragment33 Ramos C118(0.95)  LDD2202  [6]
 LDCM0596  Fragment38 Ramos C118(1.09)  LDD2203  [6]
 LDCM0566  Fragment4 Ramos C118(0.78)  LDD2184  [6]
 LDCM0610  Fragment52 Ramos C118(1.09)  LDD2204  [6]
 LDCM0614  Fragment56 Ramos C118(0.81)  LDD2205  [6]
 LDCM0569  Fragment7 Ramos C118(1.17)  LDD2186  [6]
 LDCM0571  Fragment9 Ramos C118(0.96)  LDD2188  [6]
 LDCM0022  KB02 HEK-293T C118(1.03)  LDD1492  [5]
 LDCM0023  KB03 HEK-293T C118(1.03)  LDD1497  [5]
 LDCM0024  KB05 MONO-MAC-6 C118(1.45)  LDD3335  [1]
 LDCM0528  N-(4-bromophenyl)-2-cyano-N-phenylacetamide MDA-MB-231 C118(0.51)  LDD2121  [2]
 LDCM0556  Nucleophilic fragment 8a MDA-MB-231 C118(0.48)  LDD2150  [2]

References

1 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
2 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
3 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
4 Chemoproteomic capture of RNA binding activity in living cells. Nat Commun. 2023 Oct 7;14(1):6282. doi: 10.1038/s41467-023-41844-z.
Mass spectrometry data entry: PXD044625
5 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
6 Site-specific quantitative cysteine profiling with data-independent acquisition-based mass spectrometry. Methods Enzymol. 2023;679:295-322. doi: 10.1016/bs.mie.2022.07.037. Epub 2022 Sep 7.
Mass spectrometry data entry: PXD027578