Details of the Target

General Information of Target

Target ID LDTP09593
Target Name Non-structural maintenance of chromosomes element 1 homolog (NSMCE1)
Gene Name NSMCE1
Gene ID 197370
Synonyms
Non-structural maintenance of chromosomes element 1 homolog; Non-SMC element 1 homolog; EC 2.3.2.27
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MQGSTRRMGVMTDVHRRFLQLLMTHGVLEEWDVKRLQTHCYKVHDRNATVDKLEDFINNI
NSVLESLYIEIKRGVTEDDGRPIYALVNLATTSISKMATDFAENELDLFRKALELIIDSE
TGFASSTNILNLVDQLKGKKMRKKEAEQVLQKFVQNKWLIEKEGEFTLHGRAILEMEQYI
RETYPDAVKICNICHSLLIQGQSCETCGIRMHLPCVAKYFQSNAEPRCPHCNDYWPHEIP
KVFDPEKERESGVLKSNKKSLRSRQH
Target Bioclass
Enzyme
Family
NSE1 family
Subcellular location
Nucleus
Function
RING-type zinc finger-containing E3 ubiquitin ligase that assembles with melanoma antigen protein (MAGE) to catalyze the direct transfer of ubiquitin from E2 ubiquitin-conjugating enzyme to a specific substrate. Within MAGE-RING ubiquitin ligase complex, MAGE stimulates and specifies ubiquitin ligase activity likely through recruitment and/or stabilization of the E2 ubiquitin-conjugating enzyme at the E3:substrate complex. Involved in maintenance of genome integrity, DNA damage response and DNA repair. NSMCE3/MAGEG1 and NSMCE1 ubiquitin ligase are components of SMC5-SMC6 complex and may positively regulate homologous recombination-mediated DNA repair. MAGEF1-NSMCE1 ubiquitin ligase promotes proteasomal degradation of MMS19, a key component of the cytosolic iron-sulfur protein assembly (CIA) machinery. Down-regulation of MMS19 impairs the activity of several DNA repair and metabolism enzymes such as ERCC2/XPD, FANCJ, RTEL1 and POLD1 that require iron-sulfur clusters as cofactors.
Uniprot ID
Q8WV22
Ensemble ID
ENST00000361439.9
HGNC ID
HGNC:29897

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 7 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C215(1.75)  LDD3332  [1]
BTD
 Probe Info  		C40(2.71)  LDD1700  [2]
4-Iodoacetamidophenylacetylene
 Probe Info  		C40(0.00); C215(0.00)  LDD0038  [3]
IA-alkyne
 Probe Info  		C215(0.00); C40(0.00)  LDD0036  [3]
Lodoacetamide azide
 Probe Info  		N.A.  LDD0037  [3]
AOyne
 Probe Info  		15.00  LDD0443  [4]
NAIA_5
 Probe Info  		N.A.  LDD2223  [5]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0524  2-Cyano-N-(2-morpholin-4-yl-ethyl)-acetamide MDA-MB-231 C40(0.74)  LDD2117  [2]
 LDCM0214  AC1 HEK-293T C40(1.03); C215(0.81)  LDD1507  [6]
 LDCM0215  AC10 HEK-293T C215(0.88)  LDD1508  [6]
 LDCM0226  AC11 HEK-293T C215(1.03)  LDD1509  [6]
 LDCM0237  AC12 HEK-293T C40(1.16)  LDD1510  [6]
 LDCM0259  AC14 HEK-293T C40(1.02); C215(1.06)  LDD1512  [6]
 LDCM0270  AC15 HEK-293T C40(1.04); C215(0.89)  LDD1513  [6]
 LDCM0276  AC17 HEK-293T C40(1.04); C215(0.93)  LDD1515  [6]
 LDCM0277  AC18 HEK-293T C215(0.92)  LDD1516  [6]
 LDCM0278  AC19 HEK-293T C215(0.97)  LDD1517  [6]
 LDCM0279  AC2 HEK-293T C215(0.83)  LDD1518  [6]
 LDCM0280  AC20 HEK-293T C40(1.10)  LDD1519  [6]
 LDCM0282  AC22 HEK-293T C40(1.07); C215(0.89)  LDD1521  [6]
 LDCM0283  AC23 HEK-293T C40(0.98); C215(0.88)  LDD1522  [6]
 LDCM0284  AC24 HEK-293T C215(1.00)  LDD1523  [6]
 LDCM0285  AC25 HEK-293T C40(1.02); C215(0.99)  LDD1524  [6]
 LDCM0286  AC26 HEK-293T C215(0.90)  LDD1525  [6]
 LDCM0287  AC27 HEK-293T C215(0.91)  LDD1526  [6]
 LDCM0288  AC28 HEK-293T C40(0.71)  LDD1527  [6]
 LDCM0290  AC3 HEK-293T C215(0.99)  LDD1529  [6]
 LDCM0291  AC30 HEK-293T C40(1.02); C215(1.03)  LDD1530  [6]
 LDCM0292  AC31 HEK-293T C40(1.10); C215(1.00)  LDD1531  [6]
 LDCM0293  AC32 HEK-293T C215(1.02)  LDD1532  [6]
 LDCM0294  AC33 HEK-293T C40(1.10); C215(0.81)  LDD1533  [6]
 LDCM0295  AC34 HEK-293T C215(0.82)  LDD1534  [6]
 LDCM0296  AC35 HEK-293T C215(0.90)  LDD1535  [6]
 LDCM0297  AC36 HEK-293T C40(0.99)  LDD1536  [6]
 LDCM0299  AC38 HEK-293T C40(1.08); C215(1.06)  LDD1538  [6]
 LDCM0300  AC39 HEK-293T C40(0.99); C215(1.04)  LDD1539  [6]
 LDCM0301  AC4 HEK-293T C40(1.11)  LDD1540  [6]
 LDCM0302  AC40 HEK-293T C215(1.00)  LDD1541  [6]
 LDCM0303  AC41 HEK-293T C40(1.05); C215(0.89)  LDD1542  [6]
 LDCM0304  AC42 HEK-293T C215(0.84)  LDD1543  [6]
 LDCM0305  AC43 HEK-293T C215(0.97)  LDD1544  [6]
 LDCM0306  AC44 HEK-293T C40(0.96)  LDD1545  [6]
 LDCM0308  AC46 HEK-293T C40(1.05); C215(1.04)  LDD1547  [6]
 LDCM0309  AC47 HEK-293T C40(1.03); C215(1.00)  LDD1548  [6]
 LDCM0310  AC48 HEK-293T C215(1.13)  LDD1549  [6]
 LDCM0311  AC49 HEK-293T C40(1.06); C215(0.95)  LDD1550  [6]
 LDCM0313  AC50 HEK-293T C215(0.87)  LDD1552  [6]
 LDCM0314  AC51 HEK-293T C215(0.95)  LDD1553  [6]
 LDCM0315  AC52 HEK-293T C40(1.17)  LDD1554  [6]
 LDCM0317  AC54 HEK-293T C40(1.04); C215(0.92)  LDD1556  [6]
 LDCM0318  AC55 HEK-293T C40(1.04); C215(0.87)  LDD1557  [6]
 LDCM0319  AC56 HEK-293T C215(0.93)  LDD1558  [6]
 LDCM0320  AC57 HEK-293T C40(1.04); C215(0.86)  LDD1559  [6]
 LDCM0321  AC58 HEK-293T C215(0.83)  LDD1560  [6]
 LDCM0322  AC59 HEK-293T C215(1.03)  LDD1561  [6]
 LDCM0323  AC6 HEK-293T C40(1.02); C215(1.09)  LDD1562  [6]
 LDCM0324  AC60 HEK-293T C40(0.88)  LDD1563  [6]
 LDCM0326  AC62 HEK-293T C40(0.98); C215(0.98)  LDD1565  [6]
 LDCM0327  AC63 HEK-293T C40(1.00); C215(0.95)  LDD1566  [6]
 LDCM0328  AC64 HEK-293T C215(1.01)  LDD1567  [6]
 LDCM0334  AC7 HEK-293T C40(1.02); C215(1.04)  LDD1568  [6]
 LDCM0345  AC8 HEK-293T C215(1.11)  LDD1569  [6]
 LDCM0356  AKOS034007680 HEK-293T C40(1.02); C215(0.96)  LDD1570  [6]
 LDCM0275  AKOS034007705 HEK-293T C215(0.95)  LDD1514  [6]
 LDCM0368  CL10 HEK-293T C40(1.00); C215(0.85)  LDD1572  [6]
 LDCM0369  CL100 HEK-293T C40(1.04); C215(0.99)  LDD1573  [6]
 LDCM0371  CL102 HEK-293T C40(1.04); C215(1.23)  LDD1575  [6]
 LDCM0372  CL103 HEK-293T C215(1.20)  LDD1576  [6]
 LDCM0373  CL104 HEK-293T C40(1.04); C215(0.92)  LDD1577  [6]
 LDCM0375  CL106 HEK-293T C40(1.02); C215(1.07)  LDD1579  [6]
 LDCM0376  CL107 HEK-293T C215(1.01)  LDD1580  [6]
 LDCM0377  CL108 HEK-293T C40(1.03); C215(1.00)  LDD1581  [6]
 LDCM0379  CL11 HEK-293T C40(1.02); C215(1.08)  LDD1583  [6]
 LDCM0380  CL110 HEK-293T C40(1.00); C215(1.20)  LDD1584  [6]
 LDCM0381  CL111 HEK-293T C215(1.11)  LDD1585  [6]
 LDCM0382  CL112 HEK-293T C40(1.00); C215(0.85)  LDD1586  [6]
 LDCM0384  CL114 HEK-293T C40(1.04); C215(1.20)  LDD1588  [6]
 LDCM0385  CL115 HEK-293T C215(1.29)  LDD1589  [6]
 LDCM0386  CL116 HEK-293T C40(1.08); C215(0.97)  LDD1590  [6]
 LDCM0388  CL118 HEK-293T C40(1.00); C215(1.11)  LDD1592  [6]
 LDCM0389  CL119 HEK-293T C215(1.25)  LDD1593  [6]
 LDCM0390  CL12 HEK-293T C215(0.95)  LDD1594  [6]
 LDCM0391  CL120 HEK-293T C40(1.01); C215(1.14)  LDD1595  [6]
 LDCM0393  CL122 HEK-293T C40(1.08); C215(1.03)  LDD1597  [6]
 LDCM0394  CL123 HEK-293T C215(1.33)  LDD1598  [6]
 LDCM0395  CL124 HEK-293T C40(0.97); C215(0.90)  LDD1599  [6]
 LDCM0397  CL126 HEK-293T C40(1.03); C215(1.04)  LDD1601  [6]
 LDCM0398  CL127 HEK-293T C215(1.09)  LDD1602  [6]
 LDCM0399  CL128 HEK-293T C40(0.99); C215(0.92)  LDD1603  [6]
 LDCM0401  CL14 HEK-293T C40(0.94); C215(1.16)  LDD1605  [6]
 LDCM0402  CL15 HEK-293T C215(1.11)  LDD1606  [6]
 LDCM0403  CL16 HEK-293T C40(1.10); C215(1.03)  LDD1607  [6]
 LDCM0404  CL17 HEK-293T C40(0.95); C215(0.96)  LDD1608  [6]
 LDCM0405  CL18 HEK-293T C215(0.83)  LDD1609  [6]
 LDCM0406  CL19 HEK-293T C215(0.93)  LDD1610  [6]
 LDCM0407  CL2 HEK-293T C40(1.00); C215(1.10)  LDD1611  [6]
 LDCM0408  CL20 HEK-293T C40(0.94)  LDD1612  [6]
 LDCM0410  CL22 HEK-293T C40(0.99); C215(0.99)  LDD1614  [6]
 LDCM0411  CL23 HEK-293T C40(1.07); C215(1.11)  LDD1615  [6]
 LDCM0412  CL24 HEK-293T C215(1.15)  LDD1616  [6]
 LDCM0414  CL26 HEK-293T C40(1.06); C215(1.07)  LDD1618  [6]
 LDCM0415  CL27 HEK-293T C215(1.14)  LDD1619  [6]
 LDCM0416  CL28 HEK-293T C40(1.02); C215(0.93)  LDD1620  [6]
 LDCM0417  CL29 HEK-293T C40(1.01); C215(0.97)  LDD1621  [6]
 LDCM0418  CL3 HEK-293T C215(1.21)  LDD1622  [6]
 LDCM0419  CL30 HEK-293T C215(0.88)  LDD1623  [6]
 LDCM0420  CL31 HEK-293T C215(0.95)  LDD1624  [6]
 LDCM0421  CL32 HEK-293T C40(0.96)  LDD1625  [6]
 LDCM0423  CL34 HEK-293T C40(0.95); C215(1.10)  LDD1627  [6]
 LDCM0424  CL35 HEK-293T C40(0.99); C215(0.94)  LDD1628  [6]
 LDCM0425  CL36 HEK-293T C215(0.96)  LDD1629  [6]
 LDCM0428  CL39 HEK-293T C215(0.98)  LDD1632  [6]
 LDCM0429  CL4 HEK-293T C40(1.06); C215(0.89)  LDD1633  [6]
 LDCM0430  CL40 HEK-293T C40(1.00); C215(0.90)  LDD1634  [6]
 LDCM0431  CL41 HEK-293T C40(1.03); C215(0.99)  LDD1635  [6]
 LDCM0432  CL42 HEK-293T C215(0.82)  LDD1636  [6]
 LDCM0433  CL43 HEK-293T C215(1.02)  LDD1637  [6]
 LDCM0434  CL44 HEK-293T C40(0.97)  LDD1638  [6]
 LDCM0436  CL46 HEK-293T C40(1.00); C215(1.01)  LDD1640  [6]
 LDCM0437  CL47 HEK-293T C40(0.95); C215(1.02)  LDD1641  [6]
 LDCM0438  CL48 HEK-293T C215(0.98)  LDD1642  [6]
 LDCM0440  CL5 HEK-293T C40(1.01); C215(0.87)  LDD1644  [6]
 LDCM0441  CL50 HEK-293T C40(1.00); C215(1.02)  LDD1645  [6]
 LDCM0443  CL52 HEK-293T C40(1.05); C215(1.10)  LDD1646  [6]
 LDCM0444  CL53 HEK-293T C40(1.12); C215(1.07)  LDD1647  [6]
 LDCM0445  CL54 HEK-293T C215(0.83)  LDD1648  [6]
 LDCM0446  CL55 HEK-293T C215(0.97)  LDD1649  [6]
 LDCM0447  CL56 HEK-293T C40(1.02)  LDD1650  [6]
 LDCM0449  CL58 HEK-293T C40(0.98); C215(0.89)  LDD1652  [6]
 LDCM0450  CL59 HEK-293T C40(1.01); C215(1.13)  LDD1653  [6]
 LDCM0451  CL6 HEK-293T C215(0.93)  LDD1654  [6]
 LDCM0452  CL60 HEK-293T C215(0.97)  LDD1655  [6]
 LDCM0454  CL62 HEK-293T C40(1.03); C215(1.25)  LDD1657  [6]
 LDCM0455  CL63 HEK-293T C215(1.14)  LDD1658  [6]
 LDCM0456  CL64 HEK-293T C40(1.00); C215(0.96)  LDD1659  [6]
 LDCM0457  CL65 HEK-293T C40(1.00); C215(0.87)  LDD1660  [6]
 LDCM0458  CL66 HEK-293T C215(0.79)  LDD1661  [6]
 LDCM0459  CL67 HEK-293T C215(0.95)  LDD1662  [6]
 LDCM0460  CL68 HEK-293T C40(0.95)  LDD1663  [6]
 LDCM0462  CL7 HEK-293T C215(1.03)  LDD1665  [6]
 LDCM0463  CL70 HEK-293T C40(0.96); C215(1.04)  LDD1666  [6]
 LDCM0464  CL71 HEK-293T C40(0.96); C215(1.07)  LDD1667  [6]
 LDCM0465  CL72 HEK-293T C215(0.99)  LDD1668  [6]
 LDCM0467  CL74 HEK-293T C40(0.96); C215(1.03)  LDD1670  [6]
 LDCM0469  CL76 HEK-293T C40(1.02); C215(0.92)  LDD1672  [6]
 LDCM0470  CL77 HEK-293T C40(0.99); C215(0.94)  LDD1673  [6]
 LDCM0471  CL78 HEK-293T C215(0.84)  LDD1674  [6]
 LDCM0472  CL79 HEK-293T C215(1.12)  LDD1675  [6]
 LDCM0473  CL8 HEK-293T C40(1.08)  LDD1676  [6]
 LDCM0474  CL80 HEK-293T C40(0.76)  LDD1677  [6]
 LDCM0476  CL82 HEK-293T C40(0.98); C215(1.08)  LDD1679  [6]
 LDCM0477  CL83 HEK-293T C40(0.96); C215(0.98)  LDD1680  [6]
 LDCM0478  CL84 HEK-293T C215(1.12)  LDD1681  [6]
 LDCM0480  CL86 HEK-293T C40(1.06); C215(1.16)  LDD1683  [6]
 LDCM0481  CL87 HEK-293T C215(1.14)  LDD1684  [6]
 LDCM0482  CL88 HEK-293T C40(0.99); C215(0.81)  LDD1685  [6]
 LDCM0483  CL89 HEK-293T C40(0.99); C215(0.92)  LDD1686  [6]
 LDCM0485  CL90 HEK-293T C215(0.72)  LDD1688  [6]
 LDCM0486  CL91 HEK-293T C215(1.13)  LDD1689  [6]
 LDCM0487  CL92 HEK-293T C40(0.74)  LDD1690  [6]
 LDCM0489  CL94 HEK-293T C40(0.98); C215(0.94)  LDD1692  [6]
 LDCM0490  CL95 HEK-293T C40(1.02); C215(0.98)  LDD1693  [6]
 LDCM0491  CL96 HEK-293T C215(1.04)  LDD1694  [6]
 LDCM0493  CL98 HEK-293T C40(1.01); C215(1.10)  LDD1696  [6]
 LDCM0494  CL99 HEK-293T C215(1.13)  LDD1697  [6]
 LDCM0495  E2913 HEK-293T C215(1.18)  LDD1698  [6]
 LDCM0468  Fragment33 HEK-293T C215(1.10)  LDD1671  [6]
 LDCM0427  Fragment51 HEK-293T C40(0.97); C215(0.95)  LDD1631  [6]
 LDCM0022  KB02 HEK-293T C40(0.96)  LDD1492  [6]
 LDCM0023  KB03 HEK-293T C40(0.96)  LDD1497  [6]
 LDCM0024  KB05 MKN45 C215(1.75)  LDD3332  [1]
 LDCM0506  Nucleophilic fragment 16a MDA-MB-231 C40(1.25)  LDD2099  [2]
 LDCM0514  Nucleophilic fragment 20a MDA-MB-231 C40(0.81)  LDD2107  [2]
 LDCM0516  Nucleophilic fragment 21a MDA-MB-231 C40(0.70)  LDD2109  [2]
 LDCM0518  Nucleophilic fragment 22a MDA-MB-231 C40(1.02)  LDD2111  [2]
 LDCM0526  Nucleophilic fragment 26a MDA-MB-231 C215(2.19)  LDD2119  [2]
 LDCM0530  Nucleophilic fragment 28a MDA-MB-231 C40(0.84)  LDD2123  [2]
 LDCM0532  Nucleophilic fragment 29a MDA-MB-231 C40(0.91)  LDD2125  [2]
 LDCM0534  Nucleophilic fragment 30a MDA-MB-231 C40(0.88)  LDD2127  [2]
 LDCM0536  Nucleophilic fragment 31 MDA-MB-231 C40(1.12)  LDD2129  [2]
 LDCM0542  Nucleophilic fragment 37 MDA-MB-231 C40(1.01)  LDD2135  [2]
 LDCM0543  Nucleophilic fragment 38 MDA-MB-231 C40(1.03)  LDD2136  [2]
 LDCM0544  Nucleophilic fragment 39 MDA-MB-231 C40(0.92)  LDD2137  [2]
 LDCM0211  Nucleophilic fragment 3b MDA-MB-231 C40(2.71)  LDD1700  [2]
 LDCM0546  Nucleophilic fragment 40 MDA-MB-231 C40(0.76)  LDD2140  [2]
 LDCM0556  Nucleophilic fragment 8a MDA-MB-231 C40(0.42)  LDD2150  [2]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Other
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Structural maintenance of chromosomes protein 6 (SMC6) SMC family Q96SB8
Non-structural maintenance of chromosomes element 3 homolog (NSMCE3) . Q96MG7

References

1 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
2 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
3 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
4 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.
5 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
6 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402