Details of the Target

General Information of Target

Target ID LDTP09536
Target Name Apoptosis-enhancing nuclease (AEN)
Gene Name AEN
Gene ID 64782
Synonyms
ISG20L1; Apoptosis-enhancing nuclease; EC 3.1.-.-; Interferon-stimulated 20 kDa exonuclease-like 1
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MVPREAPESAQCLCPSLTIPNAKDVLRKRHKRRSRQHQRFMARKALLQEQGLLSMPPEPG
SSPLPTPFGAATATEAASSGKQCLRAGSGSAPCSRRPAPGKASGPLPSKCVAIDCEMVGT
GPRGRVSELARCSIVSYHGNVLYDKYIRPEMPIADYRTRWSGITRQHMRKAVPFQVAQKE
ILKLLKGKVVVGHALHNDFQALKYVHPRSQTRDTTYVPNFLSEPGLHTRARVSLKDLALQ
LLHKKIQVGQHGHSSVEDATTAMELYRLVEVQWEQQEARSLWTCPEDREPDSSTDMEQYM
EDQYWPDDLAHGSRGGAREAQDRRN
Target Bioclass
Enzyme
Subcellular location
Nucleus
Function
Exonuclease with activity against single- and double-stranded DNA and RNA. Mediates p53-induced apoptosis. When induced by p53 following DNA damage, digests double-stranded DNA to form single-stranded DNA and amplifies DNA damage signals, leading to enhancement of apoptosis.
Uniprot ID
Q8WTP8
Ensemble ID
ENST00000332810.4
HGNC ID
HGNC:25722

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
CAL148 SNV: p.E319Ter .
HCT116 SNV: p.P99H .
HCT15 SNV: p.Y137C .
KMRC1 SNV: p.R279H .
LU65 SNV: p.R4L .
MEWO SNV: p.P3L .
YSCCC Insertion: p.Q36DfsTer19 .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 4 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C93(2.83)  LDD3311  [1]
CY4
 Probe Info  		K179(0.00); K183(0.00)  LDD0247  [2]
TFBX
 Probe Info  		N.A.  LDD0027  [3]
IPM
 Probe Info  		N.A.  LDD0147  [3]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0237  AC12 HEK-293T C93(0.77)  LDD1510  [4]
 LDCM0270  AC15 HEK-293T C93(0.50)  LDD1513  [4]
 LDCM0280  AC20 HEK-293T C93(0.76)  LDD1519  [4]
 LDCM0281  AC21 HEK-293T C93(0.87)  LDD1520  [4]
 LDCM0283  AC23 HEK-293T C93(0.61)  LDD1522  [4]
 LDCM0284  AC24 HEK-293T C93(0.80)  LDD1523  [4]
 LDCM0288  AC28 HEK-293T C93(0.89)  LDD1527  [4]
 LDCM0289  AC29 HEK-293T C93(0.83)  LDD1528  [4]
 LDCM0292  AC31 HEK-293T C93(0.65)  LDD1531  [4]
 LDCM0293  AC32 HEK-293T C93(0.96)  LDD1532  [4]
 LDCM0297  AC36 HEK-293T C93(0.81)  LDD1536  [4]
 LDCM0298  AC37 HEK-293T C93(0.99)  LDD1537  [4]
 LDCM0300  AC39 HEK-293T C93(0.53)  LDD1539  [4]
 LDCM0301  AC4 HEK-293T C93(1.00)  LDD1540  [4]
 LDCM0302  AC40 HEK-293T C93(0.91)  LDD1541  [4]
 LDCM0306  AC44 HEK-293T C93(0.72)  LDD1545  [4]
 LDCM0307  AC45 HEK-293T C93(0.83)  LDD1546  [4]
 LDCM0309  AC47 HEK-293T C93(0.53)  LDD1548  [4]
 LDCM0310  AC48 HEK-293T C93(0.67)  LDD1549  [4]
 LDCM0312  AC5 HEK-293T C93(1.02)  LDD1551  [4]
 LDCM0315  AC52 HEK-293T C93(0.80)  LDD1554  [4]
 LDCM0316  AC53 HEK-293T C93(0.77)  LDD1555  [4]
 LDCM0318  AC55 HEK-293T C93(0.41)  LDD1557  [4]
 LDCM0319  AC56 HEK-293T C93(0.73)  LDD1558  [4]
 LDCM0324  AC60 HEK-293T C93(1.22)  LDD1563  [4]
 LDCM0325  AC61 HEK-293T C93(1.09)  LDD1564  [4]
 LDCM0327  AC63 HEK-293T C93(1.04)  LDD1566  [4]
 LDCM0328  AC64 HEK-293T C93(0.74)  LDD1567  [4]
 LDCM0334  AC7 HEK-293T C93(0.80)  LDD1568  [4]
 LDCM0345  AC8 HEK-293T C93(1.16)  LDD1569  [4]
 LDCM0248  AKOS034007472 HEK-293T C93(0.83)  LDD1511  [4]
 LDCM0275  AKOS034007705 HEK-293T C93(0.55)  LDD1514  [4]
 LDCM0367  CL1 HEK-293T C93(1.30)  LDD1571  [4]
 LDCM0369  CL100 HEK-293T C93(0.73)  LDD1573  [4]
 LDCM0370  CL101 HEK-293T C93(0.80)  LDD1574  [4]
 LDCM0371  CL102 HEK-293T C93(0.61)  LDD1575  [4]
 LDCM0372  CL103 HEK-293T C93(0.59)  LDD1576  [4]
 LDCM0373  CL104 HEK-293T C93(0.68)  LDD1577  [4]
 LDCM0374  CL105 HEK-293T C93(0.81)  LDD1578  [4]
 LDCM0375  CL106 HEK-293T C93(0.55)  LDD1579  [4]
 LDCM0376  CL107 HEK-293T C93(0.60)  LDD1580  [4]
 LDCM0377  CL108 HEK-293T C93(0.62)  LDD1581  [4]
 LDCM0378  CL109 HEK-293T C93(0.68)  LDD1582  [4]
 LDCM0379  CL11 HEK-293T C93(1.21)  LDD1583  [4]
 LDCM0380  CL110 HEK-293T C93(0.56)  LDD1584  [4]
 LDCM0381  CL111 HEK-293T C93(0.80)  LDD1585  [4]
 LDCM0382  CL112 HEK-293T C93(0.67)  LDD1586  [4]
 LDCM0383  CL113 HEK-293T C93(0.91)  LDD1587  [4]
 LDCM0384  CL114 HEK-293T C93(0.73)  LDD1588  [4]
 LDCM0385  CL115 HEK-293T C93(0.71)  LDD1589  [4]
 LDCM0386  CL116 HEK-293T C93(0.68)  LDD1590  [4]
 LDCM0387  CL117 HEK-293T C93(0.71)  LDD1591  [4]
 LDCM0388  CL118 HEK-293T C93(0.53)  LDD1592  [4]
 LDCM0389  CL119 HEK-293T C93(0.63)  LDD1593  [4]
 LDCM0390  CL12 HEK-293T C93(1.24)  LDD1594  [4]
 LDCM0391  CL120 HEK-293T C93(0.73)  LDD1595  [4]
 LDCM0392  CL121 HEK-293T C93(0.65)  LDD1596  [4]
 LDCM0393  CL122 HEK-293T C93(0.44)  LDD1597  [4]
 LDCM0394  CL123 HEK-293T C93(0.48)  LDD1598  [4]
 LDCM0395  CL124 HEK-293T C93(0.42)  LDD1599  [4]
 LDCM0396  CL125 HEK-293T C93(0.69)  LDD1600  [4]
 LDCM0397  CL126 HEK-293T C93(1.28)  LDD1601  [4]
 LDCM0398  CL127 HEK-293T C93(1.07)  LDD1602  [4]
 LDCM0399  CL128 HEK-293T C93(0.85)  LDD1603  [4]
 LDCM0400  CL13 HEK-293T C93(0.94)  LDD1604  [4]
 LDCM0401  CL14 HEK-293T C93(0.69)  LDD1605  [4]
 LDCM0402  CL15 HEK-293T C93(0.62)  LDD1606  [4]
 LDCM0403  CL16 HEK-293T C93(0.91)  LDD1607  [4]
 LDCM0407  CL2 HEK-293T C93(1.28)  LDD1611  [4]
 LDCM0408  CL20 HEK-293T C93(1.02)  LDD1612  [4]
 LDCM0409  CL21 HEK-293T C93(0.90)  LDD1613  [4]
 LDCM0411  CL23 HEK-293T C93(0.73)  LDD1615  [4]
 LDCM0412  CL24 HEK-293T C93(1.06)  LDD1616  [4]
 LDCM0413  CL25 HEK-293T C93(0.88)  LDD1617  [4]
 LDCM0414  CL26 HEK-293T C93(0.71)  LDD1618  [4]
 LDCM0415  CL27 HEK-293T C93(0.74)  LDD1619  [4]
 LDCM0416  CL28 HEK-293T C93(0.65)  LDD1620  [4]
 LDCM0418  CL3 HEK-293T C93(1.00)  LDD1622  [4]
 LDCM0421  CL32 HEK-293T C93(0.69)  LDD1625  [4]
 LDCM0422  CL33 HEK-293T C93(0.75)  LDD1626  [4]
 LDCM0424  CL35 HEK-293T C93(0.70)  LDD1628  [4]
 LDCM0425  CL36 HEK-293T C93(0.90)  LDD1629  [4]
 LDCM0426  CL37 HEK-293T C93(1.29)  LDD1630  [4]
 LDCM0428  CL39 HEK-293T C93(0.72)  LDD1632  [4]
 LDCM0429  CL4 HEK-293T C93(1.33)  LDD1633  [4]
 LDCM0430  CL40 HEK-293T C93(0.90)  LDD1634  [4]
 LDCM0434  CL44 HEK-293T C93(1.04)  LDD1638  [4]
 LDCM0435  CL45 HEK-293T C93(0.86)  LDD1639  [4]
 LDCM0437  CL47 HEK-293T C93(0.93)  LDD1641  [4]
 LDCM0438  CL48 HEK-293T C93(1.16)  LDD1642  [4]
 LDCM0439  CL49 HEK-293T C93(0.98)  LDD1643  [4]
 LDCM0441  CL50 HEK-293T C93(0.79)  LDD1645  [4]
 LDCM0443  CL52 HEK-293T C93(0.89)  LDD1646  [4]
 LDCM0447  CL56 HEK-293T C93(1.02)  LDD1650  [4]
 LDCM0448  CL57 HEK-293T C93(0.89)  LDD1651  [4]
 LDCM0450  CL59 HEK-293T C93(0.59)  LDD1653  [4]
 LDCM0452  CL60 HEK-293T C93(1.03)  LDD1655  [4]
 LDCM0453  CL61 HEK-293T C93(1.27)  LDD1656  [4]
 LDCM0454  CL62 HEK-293T C93(0.80)  LDD1657  [4]
 LDCM0455  CL63 HEK-293T C93(0.82)  LDD1658  [4]
 LDCM0456  CL64 HEK-293T C93(0.81)  LDD1659  [4]
 LDCM0460  CL68 HEK-293T C93(0.82)  LDD1663  [4]
 LDCM0461  CL69 HEK-293T C93(0.82)  LDD1664  [4]
 LDCM0464  CL71 HEK-293T C93(0.50)  LDD1667  [4]
 LDCM0465  CL72 HEK-293T C93(0.92)  LDD1668  [4]
 LDCM0466  CL73 HEK-293T C93(1.04)  LDD1669  [4]
 LDCM0467  CL74 HEK-293T C93(0.73)  LDD1670  [4]
 LDCM0469  CL76 HEK-293T C93(0.78)  LDD1672  [4]
 LDCM0473  CL8 HEK-293T C93(1.65)  LDD1676  [4]
 LDCM0474  CL80 HEK-293T C93(0.79)  LDD1677  [4]
 LDCM0475  CL81 HEK-293T C93(0.71)  LDD1678  [4]
 LDCM0477  CL83 HEK-293T C93(0.62)  LDD1680  [4]
 LDCM0478  CL84 HEK-293T C93(0.81)  LDD1681  [4]
 LDCM0479  CL85 HEK-293T C93(1.09)  LDD1682  [4]
 LDCM0480  CL86 HEK-293T C93(1.21)  LDD1683  [4]
 LDCM0481  CL87 HEK-293T C93(1.10)  LDD1684  [4]
 LDCM0482  CL88 HEK-293T C93(1.10)  LDD1685  [4]
 LDCM0484  CL9 HEK-293T C93(1.31)  LDD1687  [4]
 LDCM0487  CL92 HEK-293T C93(1.09)  LDD1690  [4]
 LDCM0488  CL93 HEK-293T C93(0.95)  LDD1691  [4]
 LDCM0490  CL95 HEK-293T C93(1.02)  LDD1693  [4]
 LDCM0491  CL96 HEK-293T C93(1.38)  LDD1694  [4]
 LDCM0492  CL97 HEK-293T C93(1.28)  LDD1695  [4]
 LDCM0493  CL98 HEK-293T C93(1.10)  LDD1696  [4]
 LDCM0494  CL99 HEK-293T C93(1.05)  LDD1697  [4]
 LDCM0495  E2913 HEK-293T C93(0.79)  LDD1698  [4]
 LDCM0468  Fragment33 HEK-293T C93(0.71)  LDD1671  [4]
 LDCM0427  Fragment51 HEK-293T C93(0.92)  LDD1631  [4]
 LDCM0022  KB02 HEK-293T C93(1.05)  LDD1492  [4]
 LDCM0023  KB03 HEK-293T C93(1.03)  LDD1497  [4]
 LDCM0024  KB05 G361 C93(2.83)  LDD3311  [1]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 4 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Exosome complex component RRP43 (EXOSC8) RNase PH family Q96B26
N-acetylglucosamine-1-phosphotransferase subunits alpha/beta (GNPTAB) Stealth family Q3T906
E3 ubiquitin-protein ligase TRIM41 (TRIM41) TRIM/RBCC family Q8WV44
Zinc finger protein RFP (TRIM27) TRIM/RBCC family P14373
Transcription factor
Click To Hide/Show 7 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
DNA-binding protein Ikaros (IKZF1) Ikaros C2H2-type zinc-finger protein family Q13422
Zinc finger and BTB domain-containing protein 43 (ZBTB43) Krueppel C2H2-type zinc-finger protein family O43298
Zinc finger protein 317 (ZNF317) Krueppel C2H2-type zinc-finger protein family Q96PQ6
Zinc finger protein 473 (ZNF473) Krueppel C2H2-type zinc-finger protein family Q8WTR7
Zinc finger protein 64 (ZFP64) Krueppel C2H2-type zinc-finger protein family Q9NTW7
Zinc finger and BTB domain-containing protein 8A (ZBTB8A) . Q96BR9
Zinc finger protein 408 (ZNF408) . Q9H9D4
Other
Click To Hide/Show 19 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Afadin- and alpha-actinin-binding protein (SSX2IP) ADIP family Q9Y2D8
Keratin, type I cytoskeletal 40 (KRT40) Intermediate filament family Q6A162
KH domain-containing, RNA-binding, signal transduction-associated protein 2 (KHDRBS2) KHDRBS family Q5VWX1
Keratin-associated protein 10-5 (KRTAP10-5) KRTAP type 10 family P60370
Keratin-associated protein 10-7 (KRTAP10-7) KRTAP type 10 family P60409
Keratin-associated protein 10-8 (KRTAP10-8) KRTAP type 10 family P60410
Leucine zipper putative tumor suppressor 2 (LZTS2) LZTS2 family Q9BRK4
Microtubule-associated tumor suppressor candidate 2 (MTUS2) MTUS1 family Q5JR59
X-linked retinitis pigmentosa GTPase regulator-interacting protein 1 (RPGRIP1) RPGRIP1 family Q96KN7
RNA-binding Raly-like protein (RALYL) RRM HNRPC family Q86SE5
TLE family member 5 (TLE5) WD repeat Groucho/TLE family Q08117
Angio-associated migratory cell protein (AAMP) . Q13685
Emerin (EMD) . P50402
Huntingtin-associated protein 1 (HAP1) . P54257
KN motif and ankyrin repeat domain-containing protein 2 (KANK2) . Q63ZY3
Myomegalin (PDE4DIP) . Q5VU43
Polyhomeotic-like protein 2 (PHC2) . Q8IXK0
RNA-binding motif protein, Y chromosome, family 1 member A1 (RBMY1A1) . P0DJD3
RNA-binding motif protein, Y chromosome, family 1 member F/J (RBMY1F; RBMY1J) . Q15415

References

1 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
2 Cyclopropenone, Cyclopropeniminium Ion, and Cyclopropenethione as Novel Electrophilic Warheads for Potential Target Discovery of Triple-Negative Breast Cancer. J Med Chem. 2023 Feb 23;66(4):2851-2864. doi: 10.1021/acs.jmedchem.2c01889. Epub 2023 Feb 10.
3 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
4 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402