Details of the Target

General Information of Target

Target ID LDTP09394
Target Name Signal peptide peptidase-like 2B (SPPL2B)
Gene Name SPPL2B
Gene ID 56928
Synonyms
IMP4; KIAA1532; PSL1; Signal peptide peptidase-like 2B; SPP-like 2B; SPPL2b; EC 3.4.23.-; Intramembrane protease 4; IMP-4; Presenilin homologous protein 4; PSH4; Presenilin-like protein 1
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MAAAVAAALARLLAAFLLLAAQVACEYGMVHVVSQAGGPEGKDYCILYNPQWAHLPHDLS
KASFLQLRNWTASLLCSAADLPARGFSNQIPLVARGNCTFYEKVRLAQGSGARGLLIVSR
ERLVPPGGNKTQYDEIGIPVALLSYKDMLDIFTRFGRTVRAALYAPKEPVLDYNMVIIFI
MAVGTVAIGGYWAGSRDVKKRYMKHKRDDGPEKQEDEAVDVTPVMTCVFVVMCCSMLVLL
YYFYDLLVYVVIGIFCLASATGLYSCLAPCVRRLPFGKCRIPNNSLPYFHKRPQARMLLL
ALFCVAVSVVWGVFRNEDQWAWVLQDALGIAFCLYMLKTIRLPTFKACTLLLLVLFLYDI
FFVFITPFLTKSGSSIMVEVATGPSDSATREKLPMVLKVPRLNSSPLALCDRPFSLLGFG
DILVPGLLVAYCHRFDIQVQSSRVYFVACTIAYGVGLLVTFVALALMQRGQPALLYLVPC
TLVTSCAVALWRRELGVFWTGSGFAKVLPPSPWAPAPADGPQPPKDSATPLSPQPPSEEP
ATSPWPAEQSPKSRTSEEMGAGAPMREPGSPAESEGRDQAQPSPVTQPGASA
Target Bioclass
Enzyme
Family
Peptidase A22B family
Subcellular location
Cell membrane
Function
Intramembrane-cleaving aspartic protease (I-CLiP) that cleaves type II membrane signal peptides in the hydrophobic plane of the membrane. Functions in ITM2B and TNF processing. Catalyzes the intramembrane cleavage of the anchored fragment of shed TNF-alpha (TNF), which promotes the release of the intracellular domain (ICD) for signaling to the nucleus. May play a role in the regulation of innate and adaptive immunity. Catalyzes the intramembrane cleavage of the simian foamy virus processed leader peptide gp18 of the envelope glycoprotein gp130 dependently of prior ectodomain shedding by furin or furin-like proprotein convertase (PC)-mediated cleavage proteolysis.
Uniprot ID
Q8TCT7
Ensemble ID
ENST00000610743.4
HGNC ID
HGNC:30627
ChEMBL ID
CHEMBL4105912

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
AN3CA SNV: p.V444A DBIA    Probe Info 
CASKI SNV: p.E567K .
CCK81 Deletion: p.P126RfsTer34
SNV: p.A388T		 .
HCT15 SNV: p.A347T .
MCC13 SNV: p.G501S
Substitution: p.R315C		 .
MFE319 Deletion: p.P126RfsTer34 .
MOLT4 SNV: p.A258T .
RD SNV: p.R154S .
SUPT1 SNV: p.S543P .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 3 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
STPyne
 Probe Info  		K278(5.56)  LDD0277  [1]
DBIA
 Probe Info  		C165(1.12)  LDD3337  [2]
N1
 Probe Info  		N.A.  LDD0245  [3]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0214  AC1 HEK-293T C45(0.98)  LDD1507  [4]
 LDCM0215  AC10 HEK-293T C45(1.04)  LDD1508  [4]
 LDCM0237  AC12 HEK-293T C45(1.07)  LDD1510  [4]
 LDCM0259  AC14 HEK-293T C45(0.97)  LDD1512  [4]
 LDCM0270  AC15 HEK-293T C45(1.01)  LDD1513  [4]
 LDCM0276  AC17 HEK-293T C45(0.99)  LDD1515  [4]
 LDCM0277  AC18 HEK-293T C45(0.92)  LDD1516  [4]
 LDCM0279  AC2 HEK-293T C45(0.91)  LDD1518  [4]
 LDCM0280  AC20 HEK-293T C45(1.00)  LDD1519  [4]
 LDCM0281  AC21 HEK-293T C45(1.05)  LDD1520  [4]
 LDCM0282  AC22 HEK-293T C45(0.89)  LDD1521  [4]
 LDCM0283  AC23 HEK-293T C45(1.01)  LDD1522  [4]
 LDCM0284  AC24 HEK-293T C45(1.01)  LDD1523  [4]
 LDCM0285  AC25 HEK-293T C45(1.08)  LDD1524  [4]
 LDCM0286  AC26 HEK-293T C45(0.93)  LDD1525  [4]
 LDCM0288  AC28 HEK-293T C45(1.10)  LDD1527  [4]
 LDCM0289  AC29 HEK-293T C45(0.96)  LDD1528  [4]
 LDCM0291  AC30 HEK-293T C45(0.95)  LDD1530  [4]
 LDCM0292  AC31 HEK-293T C45(1.18)  LDD1531  [4]
 LDCM0293  AC32 HEK-293T C45(1.10)  LDD1532  [4]
 LDCM0294  AC33 HEK-293T C45(1.19)  LDD1533  [4]
 LDCM0295  AC34 HEK-293T C45(0.84)  LDD1534  [4]
 LDCM0297  AC36 HEK-293T C45(0.94)  LDD1536  [4]
 LDCM0298  AC37 HEK-293T C45(1.04)  LDD1537  [4]
 LDCM0299  AC38 HEK-293T C45(0.99)  LDD1538  [4]
 LDCM0300  AC39 HEK-293T C45(1.13)  LDD1539  [4]
 LDCM0301  AC4 HEK-293T C45(1.08)  LDD1540  [4]
 LDCM0302  AC40 HEK-293T C45(1.20)  LDD1541  [4]
 LDCM0303  AC41 HEK-293T C45(1.14)  LDD1542  [4]
 LDCM0304  AC42 HEK-293T C45(0.99)  LDD1543  [4]
 LDCM0306  AC44 HEK-293T C45(1.17)  LDD1545  [4]
 LDCM0307  AC45 HEK-293T C45(0.98)  LDD1546  [4]
 LDCM0308  AC46 HEK-293T C45(0.97)  LDD1547  [4]
 LDCM0309  AC47 HEK-293T C45(1.03)  LDD1548  [4]
 LDCM0310  AC48 HEK-293T C45(1.04)  LDD1549  [4]
 LDCM0311  AC49 HEK-293T C45(0.95)  LDD1550  [4]
 LDCM0312  AC5 HEK-293T C45(1.04)  LDD1551  [4]
 LDCM0313  AC50 HEK-293T C45(0.87)  LDD1552  [4]
 LDCM0315  AC52 HEK-293T C45(1.08)  LDD1554  [4]
 LDCM0316  AC53 HEK-293T C45(1.02)  LDD1555  [4]
 LDCM0317  AC54 HEK-293T C45(1.02)  LDD1556  [4]
 LDCM0318  AC55 HEK-293T C45(1.08)  LDD1557  [4]
 LDCM0319  AC56 HEK-293T C45(1.06)  LDD1558  [4]
 LDCM0320  AC57 HEK-293T C45(1.20)  LDD1559  [4]
 LDCM0321  AC58 HEK-293T C45(1.09)  LDD1560  [4]
 LDCM0323  AC6 HEK-293T C45(0.98)  LDD1562  [4]
 LDCM0324  AC60 HEK-293T C45(1.07)  LDD1563  [4]
 LDCM0325  AC61 HEK-293T C45(0.98)  LDD1564  [4]
 LDCM0326  AC62 HEK-293T C45(1.01)  LDD1565  [4]
 LDCM0327  AC63 HEK-293T C45(1.06)  LDD1566  [4]
 LDCM0328  AC64 HEK-293T C45(1.02)  LDD1567  [4]
 LDCM0334  AC7 HEK-293T C45(1.11)  LDD1568  [4]
 LDCM0345  AC8 HEK-293T C45(1.02)  LDD1569  [4]
 LDCM0248  AKOS034007472 HEK-293T C45(1.11)  LDD1511  [4]
 LDCM0356  AKOS034007680 HEK-293T C45(1.02)  LDD1570  [4]
 LDCM0275  AKOS034007705 HEK-293T C45(1.05)  LDD1514  [4]
 LDCM0367  CL1 HEK-293T C45(0.96)  LDD1571  [4]
 LDCM0368  CL10 HEK-293T C45(0.91)  LDD1572  [4]
 LDCM0369  CL100 HEK-293T C45(1.06)  LDD1573  [4]
 LDCM0370  CL101 HEK-293T C45(0.88)  LDD1574  [4]
 LDCM0371  CL102 HEK-293T C45(1.08)  LDD1575  [4]
 LDCM0372  CL103 HEK-293T C45(0.97)  LDD1576  [4]
 LDCM0373  CL104 HEK-293T C45(1.04)  LDD1577  [4]
 LDCM0374  CL105 HEK-293T C45(1.03)  LDD1578  [4]
 LDCM0375  CL106 HEK-293T C45(1.16)  LDD1579  [4]
 LDCM0376  CL107 HEK-293T C45(0.91)  LDD1580  [4]
 LDCM0377  CL108 HEK-293T C45(1.10)  LDD1581  [4]
 LDCM0378  CL109 HEK-293T C45(0.91)  LDD1582  [4]
 LDCM0379  CL11 HEK-293T C45(1.03)  LDD1583  [4]
 LDCM0380  CL110 HEK-293T C45(1.31)  LDD1584  [4]
 LDCM0381  CL111 HEK-293T C45(0.97)  LDD1585  [4]
 LDCM0382  CL112 HEK-293T C45(1.11)  LDD1586  [4]
 LDCM0383  CL113 HEK-293T C45(1.10)  LDD1587  [4]
 LDCM0384  CL114 HEK-293T C45(1.19)  LDD1588  [4]
 LDCM0385  CL115 HEK-293T C45(0.87)  LDD1589  [4]
 LDCM0386  CL116 HEK-293T C45(1.11)  LDD1590  [4]
 LDCM0387  CL117 HEK-293T C45(0.98)  LDD1591  [4]
 LDCM0388  CL118 HEK-293T C45(1.28)  LDD1592  [4]
 LDCM0389  CL119 HEK-293T C45(0.94)  LDD1593  [4]
 LDCM0390  CL12 HEK-293T C45(1.18)  LDD1594  [4]
 LDCM0391  CL120 HEK-293T C45(1.12)  LDD1595  [4]
 LDCM0392  CL121 HEK-293T C45(0.89)  LDD1596  [4]
 LDCM0393  CL122 HEK-293T C45(1.27)  LDD1597  [4]
 LDCM0394  CL123 HEK-293T C45(0.92)  LDD1598  [4]
 LDCM0395  CL124 HEK-293T C45(1.16)  LDD1599  [4]
 LDCM0396  CL125 HEK-293T C45(1.00)  LDD1600  [4]
 LDCM0397  CL126 HEK-293T C45(1.32)  LDD1601  [4]
 LDCM0398  CL127 HEK-293T C45(0.93)  LDD1602  [4]
 LDCM0399  CL128 HEK-293T C45(1.03)  LDD1603  [4]
 LDCM0400  CL13 HEK-293T C45(1.03)  LDD1604  [4]
 LDCM0401  CL14 HEK-293T C45(1.18)  LDD1605  [4]
 LDCM0402  CL15 HEK-293T C45(0.92)  LDD1606  [4]
 LDCM0403  CL16 HEK-293T C45(1.11)  LDD1607  [4]
 LDCM0404  CL17 HEK-293T C45(1.15)  LDD1608  [4]
 LDCM0405  CL18 HEK-293T C45(0.89)  LDD1609  [4]
 LDCM0407  CL2 HEK-293T C45(1.17)  LDD1611  [4]
 LDCM0408  CL20 HEK-293T C45(0.99)  LDD1612  [4]
 LDCM0409  CL21 HEK-293T C45(1.07)  LDD1613  [4]
 LDCM0410  CL22 HEK-293T C45(1.01)  LDD1614  [4]
 LDCM0411  CL23 HEK-293T C45(0.97)  LDD1615  [4]
 LDCM0412  CL24 HEK-293T C45(1.01)  LDD1616  [4]
 LDCM0413  CL25 HEK-293T C45(1.02)  LDD1617  [4]
 LDCM0414  CL26 HEK-293T C45(1.28)  LDD1618  [4]
 LDCM0415  CL27 HEK-293T C45(0.91)  LDD1619  [4]
 LDCM0416  CL28 HEK-293T C45(1.25)  LDD1620  [4]
 LDCM0417  CL29 HEK-293T C45(1.10)  LDD1621  [4]
 LDCM0418  CL3 HEK-293T C45(0.88)  LDD1622  [4]
 LDCM0419  CL30 HEK-293T C45(0.99)  LDD1623  [4]
 LDCM0421  CL32 HEK-293T C45(0.93)  LDD1625  [4]
 LDCM0422  CL33 HEK-293T C45(1.13)  LDD1626  [4]
 LDCM0423  CL34 HEK-293T C45(0.93)  LDD1627  [4]
 LDCM0424  CL35 HEK-293T C45(1.25)  LDD1628  [4]
 LDCM0425  CL36 HEK-293T C45(0.91)  LDD1629  [4]
 LDCM0426  CL37 HEK-293T C45(0.92)  LDD1630  [4]
 LDCM0428  CL39 HEK-293T C45(0.91)  LDD1632  [4]
 LDCM0429  CL4 HEK-293T C45(1.25)  LDD1633  [4]
 LDCM0430  CL40 HEK-293T C45(0.97)  LDD1634  [4]
 LDCM0431  CL41 HEK-293T C45(0.98)  LDD1635  [4]
 LDCM0432  CL42 HEK-293T C45(0.82)  LDD1636  [4]
 LDCM0434  CL44 HEK-293T C45(1.00)  LDD1638  [4]
 LDCM0435  CL45 HEK-293T C45(0.97)  LDD1639  [4]
 LDCM0436  CL46 HEK-293T C45(0.98)  LDD1640  [4]
 LDCM0437  CL47 HEK-293T C45(1.21)  LDD1641  [4]
 LDCM0438  CL48 HEK-293T C45(0.88)  LDD1642  [4]
 LDCM0439  CL49 HEK-293T C45(0.84)  LDD1643  [4]
 LDCM0440  CL5 HEK-293T C45(1.13)  LDD1644  [4]
 LDCM0441  CL50 HEK-293T C45(1.24)  LDD1645  [4]
 LDCM0443  CL52 HEK-293T C45(1.17)  LDD1646  [4]
 LDCM0444  CL53 HEK-293T C45(1.12)  LDD1647  [4]
 LDCM0445  CL54 HEK-293T C45(1.14)  LDD1648  [4]
 LDCM0447  CL56 HEK-293T C45(0.94)  LDD1650  [4]
 LDCM0448  CL57 HEK-293T C45(1.06)  LDD1651  [4]
 LDCM0449  CL58 HEK-293T C45(1.01)  LDD1652  [4]
 LDCM0450  CL59 HEK-293T C45(1.04)  LDD1653  [4]
 LDCM0451  CL6 HEK-293T C45(1.11)  LDD1654  [4]
 LDCM0452  CL60 HEK-293T C45(0.98)  LDD1655  [4]
 LDCM0453  CL61 HEK-293T C45(0.92)  LDD1656  [4]
 LDCM0454  CL62 HEK-293T C45(1.10)  LDD1657  [4]
 LDCM0455  CL63 HEK-293T C45(0.90)  LDD1658  [4]
 LDCM0456  CL64 HEK-293T C45(0.88)  LDD1659  [4]
 LDCM0457  CL65 HEK-293T C45(1.12)  LDD1660  [4]
 LDCM0458  CL66 HEK-293T C45(0.88)  LDD1661  [4]
 LDCM0460  CL68 HEK-293T C45(0.84)  LDD1663  [4]
 LDCM0461  CL69 HEK-293T C45(1.16)  LDD1664  [4]
 LDCM0463  CL70 HEK-293T C45(0.97)  LDD1666  [4]
 LDCM0464  CL71 HEK-293T C45(1.04)  LDD1667  [4]
 LDCM0465  CL72 HEK-293T C45(1.01)  LDD1668  [4]
 LDCM0466  CL73 HEK-293T C45(0.90)  LDD1669  [4]
 LDCM0467  CL74 HEK-293T C45(1.08)  LDD1670  [4]
 LDCM0469  CL76 HEK-293T C45(1.07)  LDD1672  [4]
 LDCM0470  CL77 HEK-293T C45(0.96)  LDD1673  [4]
 LDCM0471  CL78 HEK-293T C45(0.85)  LDD1674  [4]
 LDCM0473  CL8 HEK-293T C45(1.10)  LDD1676  [4]
 LDCM0474  CL80 HEK-293T C45(0.90)  LDD1677  [4]
 LDCM0475  CL81 HEK-293T C45(0.99)  LDD1678  [4]
 LDCM0476  CL82 HEK-293T C45(0.99)  LDD1679  [4]
 LDCM0477  CL83 HEK-293T C45(0.99)  LDD1680  [4]
 LDCM0478  CL84 HEK-293T C45(1.00)  LDD1681  [4]
 LDCM0479  CL85 HEK-293T C45(0.96)  LDD1682  [4]
 LDCM0480  CL86 HEK-293T C45(1.46)  LDD1683  [4]
 LDCM0481  CL87 HEK-293T C45(1.03)  LDD1684  [4]
 LDCM0482  CL88 HEK-293T C45(1.18)  LDD1685  [4]
 LDCM0483  CL89 HEK-293T C45(0.96)  LDD1686  [4]
 LDCM0484  CL9 HEK-293T C45(0.99)  LDD1687  [4]
 LDCM0485  CL90 HEK-293T C45(1.10)  LDD1688  [4]
 LDCM0487  CL92 HEK-293T C45(1.19)  LDD1690  [4]
 LDCM0488  CL93 HEK-293T C45(1.13)  LDD1691  [4]
 LDCM0489  CL94 HEK-293T C45(1.05)  LDD1692  [4]
 LDCM0490  CL95 HEK-293T C45(1.23)  LDD1693  [4]
 LDCM0491  CL96 HEK-293T C45(1.10)  LDD1694  [4]
 LDCM0492  CL97 HEK-293T C45(1.00)  LDD1695  [4]
 LDCM0493  CL98 HEK-293T C45(1.28)  LDD1696  [4]
 LDCM0494  CL99 HEK-293T C45(1.05)  LDD1697  [4]
 LDCM0495  E2913 HEK-293T C45(0.92)  LDD1698  [4]
 LDCM0468  Fragment33 HEK-293T C45(0.88)  LDD1671  [4]
 LDCM0427  Fragment51 HEK-293T C45(1.25)  LDD1631  [4]
 LDCM0022  KB02 A204 C165(1.38)  LDD2252  [2]
 LDCM0023  KB03 A204 C165(1.29)  LDD2669  [2]
 LDCM0024  KB05 MSTO-211H C165(1.12)  LDD3337  [2]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Ribose-phosphate pyrophosphokinase 1 (PRPS1) Ribose-phosphate pyrophosphokinase family P60891
Transcription initiation factor IIB (GTF2B) TFIIB family Q00403
Transporter and channel
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Alpha-synuclein (SNCA) Synuclein family P37840
Other
Click To Hide/Show 5 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Protein BRICK1 (BRK1) BRK1 family Q8WUW1
Protein Dr1 (DR1) NC2 beta/DR1 family Q01658
Heat shock protein beta-1 (HSPB1) Small heat shock protein (HSP20) family P04792
General transcription factor 3C polypeptide 3 (GTF3C3) . Q9Y5Q9
RING finger protein 11 (RNF11) . Q9Y3C5

References

1 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
2 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
3 Cyclopropenone, Cyclopropeniminium Ion, and Cyclopropenethione as Novel Electrophilic Warheads for Potential Target Discovery of Triple-Negative Breast Cancer. J Med Chem. 2023 Feb 23;66(4):2851-2864. doi: 10.1021/acs.jmedchem.2c01889. Epub 2023 Feb 10.
4 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402