Details of the Target

General Information of Target

Target ID LDTP09162
Target Name Microcephalin (MCPH1)
Gene Name MCPH1
Gene ID 79648
Synonyms
Microcephalin
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MAAPILKDVVAYVEVWSSNGTENYSKTFTTQLVDMGAKVSKTFNKQVTHVIFKDGYQSTW
DKAQKRGVKLVSVLWVEKCRTAGAHIDESLFPAANMNEHLSSLIKKKRKCMQPKDFNFKT
PENDKRFQKKFEKMAKELQRQKTNLDDDVPILLFESNGSLIYTPTIEINSRHHSAMEKRL
QEMKEKRENLSPTSSQMIQQSHDNPSNSLCEAPLNISRDTLCSDEYFAGGLHSSFDDLCG
NSGCGNQERKLEGSINDIKSDVCISSLVLKANNIHSSPSFTHLDKSSPQKFLSNLSKEEI
NLQRNIAGKVVTPDQKQAAGMSQETFEEKYRLSPTLSSTKGHLLIHSRPRSSSVKRKRVS
HGSHSPPKEKCKRKRSTRRSIMPRLQLCRSEDRLQHVAGPALEALSCGESSYDDYFSPDN
LKERYSENLPPESQLPSSPAQLSCRSLSKKERTSIFEMSDFSCVGKKTRTVDITNFTAKT
ISSPRKTGNGEGRATSSCVTSAPEEALRCCRQAGKEDACPEGNGFSYTIEDPALPKGHDD
DLTPLEGSLEEMKEAVGLKSTQNKGTTSKISNSSEGEAQSEHEPCFIVDCNMETSTEEKE
NLPGGYSGSVKNRPTRHDVLDDSCDGFKDLIKPHEELKKSGRGKKPTRTLVMTSMPSEKQ
NVVIQVVDKLKGFSIAPDVCETTTHVLSGKPLRTLNVLLGIARGCWVLSYDWVLWSLELG
HWISEEPFELSHHFPAAPLCRSECHLSAGPYRGTLFADQPAMFVSPASSPPVAKLCELVH
LCGGRVSQVPRQASIVIGPYSGKKKATVKYLSEKWVLDSITQHKVCAPENYLLSQ
Target Bioclass
Other
Subcellular location
Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
Function Implicated in chromosome condensation and DNA damage induced cellular responses. May play a role in neurogenesis and regulation of the size of the cerebral cortex.
Uniprot ID
Q8NEM0
Ensemble ID
ENST00000344683.10
HGNC ID
HGNC:6954

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
DEL SNV: p.K340E .
G415 SNV: p.H634R .
HT115 SNV: p.D124Y .
ICC4 SNV: p.V679F .
IGROV1 SNV: p.K553N .
Ishikawa (Heraklio) 02 ER Deletion: p.T468PfsTer32 .
LNCaP clone FGC SNV: p.A518T .
MOLT4 Deletion: p.T468PfsTer32;p.P771QfsTer8
SNV: p.T220A		 .
NCIH1155 SNV: p.T220I .
SKMEL24 SNV: p.V816L .
UACC257 Substitution: p.P544V .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 3 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C776(0.80); C782(0.80)  LDD0531  [1]
CY-1
 Probe Info  		N.A.  LDD0246  [2]
IPM
 Probe Info  		N.A.  LDD2156  [3]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0214  AC1 HCT 116 C776(0.80); C782(0.80)  LDD0531  [1]
 LDCM0215  AC10 PaTu 8988t C776(0.98); C782(0.98)  LDD1094  [1]
 LDCM0226  AC11 PaTu 8988t C776(1.05); C782(1.05)  LDD1105  [1]
 LDCM0230  AC113 HCT 116 C776(0.89); C782(0.89)  LDD0547  [1]
 LDCM0231  AC114 HCT 116 C776(1.02); C782(1.02)  LDD0548  [1]
 LDCM0232  AC115 HCT 116 C776(0.97); C782(0.97)  LDD0549  [1]
 LDCM0233  AC116 HCT 116 C776(1.16); C782(1.16)  LDD0550  [1]
 LDCM0234  AC117 HCT 116 C776(0.97); C782(0.97)  LDD0551  [1]
 LDCM0235  AC118 HCT 116 C776(0.92); C782(0.92)  LDD0552  [1]
 LDCM0236  AC119 HCT 116 C776(1.02); C782(1.02)  LDD0553  [1]
 LDCM0237  AC12 PaTu 8988t C776(0.81); C782(0.81)  LDD1116  [1]
 LDCM0238  AC120 HCT 116 C776(1.13); C782(1.13)  LDD0555  [1]
 LDCM0239  AC121 HCT 116 C776(1.30); C782(1.30)  LDD0556  [1]
 LDCM0240  AC122 HCT 116 C776(0.94); C782(0.94)  LDD0557  [1]
 LDCM0241  AC123 HCT 116 C776(0.92); C782(0.92)  LDD0558  [1]
 LDCM0242  AC124 HCT 116 C776(1.28); C782(1.28)  LDD0559  [1]
 LDCM0243  AC125 HCT 116 C776(0.90); C782(0.90)  LDD0560  [1]
 LDCM0244  AC126 HCT 116 C776(0.97); C782(0.97)  LDD0561  [1]
 LDCM0245  AC127 HCT 116 C776(1.05); C782(1.05)  LDD0562  [1]
 LDCM0246  AC128 HCT 116 C776(1.08); C782(1.08)  LDD0563  [1]
 LDCM0247  AC129 HCT 116 C776(1.30); C782(1.30)  LDD0564  [1]
 LDCM0249  AC130 HCT 116 C776(1.16); C782(1.16)  LDD0566  [1]
 LDCM0250  AC131 HCT 116 C776(1.08); C782(1.08)  LDD0567  [1]
 LDCM0251  AC132 HCT 116 C776(1.15); C782(1.15)  LDD0568  [1]
 LDCM0252  AC133 HCT 116 C776(1.80); C782(1.80)  LDD0569  [1]
 LDCM0253  AC134 HCT 116 C776(1.16); C782(1.16)  LDD0570  [1]
 LDCM0254  AC135 HCT 116 C776(1.01); C782(1.01)  LDD0571  [1]
 LDCM0255  AC136 HCT 116 C776(1.35); C782(1.35)  LDD0572  [1]
 LDCM0256  AC137 HCT 116 C776(1.11); C782(1.11)  LDD0573  [1]
 LDCM0257  AC138 HCT 116 C776(1.45); C782(1.45)  LDD0574  [1]
 LDCM0258  AC139 HCT 116 C776(1.34); C782(1.34)  LDD0575  [1]
 LDCM0259  AC14 PaTu 8988t C776(0.99); C782(0.99)  LDD1138  [1]
 LDCM0260  AC140 HCT 116 C776(1.30); C782(1.30)  LDD0577  [1]
 LDCM0261  AC141 HCT 116 C776(1.50); C782(1.50)  LDD0578  [1]
 LDCM0262  AC142 HCT 116 C776(1.23); C782(1.23)  LDD0579  [1]
 LDCM0263  AC143 HCT 116 C776(0.91); C782(0.91)  LDD0580  [1]
 LDCM0264  AC144 HCT 116 C776(0.71); C782(0.71)  LDD0581  [1]
 LDCM0265  AC145 HCT 116 C776(0.97); C782(0.97)  LDD0582  [1]
 LDCM0266  AC146 HCT 116 C776(0.89); C782(0.89)  LDD0583  [1]
 LDCM0267  AC147 HCT 116 C776(0.98); C782(0.98)  LDD0584  [1]
 LDCM0268  AC148 HCT 116 C776(0.84); C782(0.84)  LDD0585  [1]
 LDCM0269  AC149 HCT 116 C776(0.78); C782(0.78)  LDD0586  [1]
 LDCM0270  AC15 PaTu 8988t C776(1.11); C782(1.11)  LDD1149  [1]
 LDCM0271  AC150 HCT 116 C776(0.91); C782(0.91)  LDD0588  [1]
 LDCM0272  AC151 HCT 116 C776(0.94); C782(0.94)  LDD0589  [1]
 LDCM0273  AC152 HCT 116 C776(0.89); C782(0.89)  LDD0590  [1]
 LDCM0274  AC153 HCT 116 C776(0.82); C782(0.82)  LDD0591  [1]
 LDCM0621  AC154 HCT 116 C776(1.14); C782(1.14)  LDD2158  [1]
 LDCM0622  AC155 HCT 116 C776(1.00); C782(1.00)  LDD2159  [1]
 LDCM0623  AC156 HCT 116 C776(1.35); C782(1.35)  LDD2160  [1]
 LDCM0624  AC157 HCT 116 C776(0.77); C782(0.77)  LDD2161  [1]
 LDCM0276  AC17 HCT 116 C776(0.86); C782(0.86)  LDD0593  [1]
 LDCM0277  AC18 HCT 116 C776(0.92); C782(0.92)  LDD0594  [1]
 LDCM0278  AC19 HCT 116 C776(0.91); C782(0.91)  LDD0595  [1]
 LDCM0279  AC2 HCT 116 C776(0.82); C782(0.82)  LDD0596  [1]
 LDCM0280  AC20 HCT 116 C776(0.86); C782(0.86)  LDD0597  [1]
 LDCM0281  AC21 HCT 116 C776(0.79); C782(0.79)  LDD0598  [1]
 LDCM0282  AC22 HCT 116 C776(0.77); C782(0.77)  LDD0599  [1]
 LDCM0283  AC23 HCT 116 C776(0.76); C782(0.76)  LDD0600  [1]
 LDCM0284  AC24 HCT 116 C776(0.79); C782(0.79)  LDD0601  [1]
 LDCM0290  AC3 HCT 116 C776(0.92); C782(0.92)  LDD0607  [1]
 LDCM0296  AC35 HCT 116 C776(0.77); C782(0.77)  LDD0613  [1]
 LDCM0297  AC36 HCT 116 C776(1.08); C782(1.08)  LDD0614  [1]
 LDCM0298  AC37 HCT 116 C776(0.97); C782(0.97)  LDD0615  [1]
 LDCM0299  AC38 HCT 116 C776(0.92); C782(0.92)  LDD0616  [1]
 LDCM0300  AC39 HCT 116 C776(1.08); C782(1.08)  LDD0617  [1]
 LDCM0301  AC4 HCT 116 C776(0.81); C782(0.81)  LDD0618  [1]
 LDCM0302  AC40 HCT 116 C776(1.04); C782(1.04)  LDD0619  [1]
 LDCM0303  AC41 HCT 116 C776(1.03); C782(1.03)  LDD0620  [1]
 LDCM0304  AC42 HCT 116 C776(1.06); C782(1.06)  LDD0621  [1]
 LDCM0305  AC43 HCT 116 C776(1.03); C782(1.03)  LDD0622  [1]
 LDCM0306  AC44 HCT 116 C776(1.23); C782(1.23)  LDD0623  [1]
 LDCM0307  AC45 HCT 116 C776(1.08); C782(1.08)  LDD0624  [1]
 LDCM0312  AC5 HCT 116 C776(0.91); C782(0.91)  LDD0629  [1]
 LDCM0320  AC57 HCT 116 C776(0.84); C782(0.84)  LDD0637  [1]
 LDCM0321  AC58 HCT 116 C776(0.91); C782(0.91)  LDD0638  [1]
 LDCM0322  AC59 HCT 116 C776(0.84); C782(0.84)  LDD0639  [1]
 LDCM0323  AC6 PaTu 8988t C776(0.84); C782(0.84)  LDD1202  [1]
 LDCM0324  AC60 HCT 116 C776(0.78); C782(0.78)  LDD0641  [1]
 LDCM0325  AC61 HCT 116 C776(0.78); C782(0.78)  LDD0642  [1]
 LDCM0326  AC62 HCT 116 C776(0.89); C782(0.89)  LDD0643  [1]
 LDCM0327  AC63 HCT 116 C776(0.81); C782(0.81)  LDD0644  [1]
 LDCM0328  AC64 HCT 116 C776(0.86); C782(0.86)  LDD0645  [1]
 LDCM0329  AC65 HCT 116 C776(0.97); C782(0.97)  LDD0646  [1]
 LDCM0330  AC66 HCT 116 C776(1.01); C782(1.01)  LDD0647  [1]
 LDCM0331  AC67 HCT 116 C776(0.79); C782(0.79)  LDD0648  [1]
 LDCM0332  AC68 HCT 116 C776(1.09); C782(1.09)  LDD0649  [1]
 LDCM0333  AC69 HCT 116 C776(0.86); C782(0.86)  LDD0650  [1]
 LDCM0334  AC7 PaTu 8988t C776(0.75); C782(0.75)  LDD1213  [1]
 LDCM0335  AC70 HCT 116 C776(0.75); C782(0.75)  LDD0652  [1]
 LDCM0336  AC71 HCT 116 C776(0.85); C782(0.85)  LDD0653  [1]
 LDCM0337  AC72 HCT 116 C776(0.89); C782(0.89)  LDD0654  [1]
 LDCM0338  AC73 HCT 116 C776(0.93); C782(0.93)  LDD0655  [1]
 LDCM0339  AC74 HCT 116 C776(0.98); C782(0.98)  LDD0656  [1]
 LDCM0340  AC75 HCT 116 C776(1.12); C782(1.12)  LDD0657  [1]
 LDCM0341  AC76 HCT 116 C776(0.84); C782(0.84)  LDD0658  [1]
 LDCM0342  AC77 HCT 116 C776(0.92); C782(0.92)  LDD0659  [1]
 LDCM0343  AC78 HCT 116 C776(0.82); C782(0.82)  LDD0660  [1]
 LDCM0344  AC79 HCT 116 C776(0.96); C782(0.96)  LDD0661  [1]
 LDCM0345  AC8 PaTu 8988t C776(0.95); C782(0.95)  LDD1224  [1]
 LDCM0346  AC80 HCT 116 C776(0.93); C782(0.93)  LDD0663  [1]
 LDCM0347  AC81 HCT 116 C776(1.08); C782(1.08)  LDD0664  [1]
 LDCM0348  AC82 HCT 116 C776(1.09); C782(1.09)  LDD0665  [1]
 LDCM0248  AKOS034007472 PaTu 8988t C776(0.77); C782(0.77)  LDD1127  [1]
 LDCM0356  AKOS034007680 PaTu 8988t C776(0.93); C782(0.93)  LDD1235  [1]
 LDCM0275  AKOS034007705 PaTu 8988t C776(0.99); C782(0.99)  LDD1154  [1]
 LDCM0367  CL1 HCT 116 C776(0.89); C782(0.89)  LDD0684  [1]
 LDCM0368  CL10 HCT 116 C776(0.85); C782(0.85)  LDD0685  [1]
 LDCM0369  CL100 HCT 116 C776(0.86); C782(0.86)  LDD0686  [1]
 LDCM0370  CL101 PaTu 8988t C776(0.91); C782(0.91)  LDD1249  [1]
 LDCM0371  CL102 PaTu 8988t C776(0.90); C782(0.90)  LDD1250  [1]
 LDCM0372  CL103 PaTu 8988t C776(1.01); C782(1.01)  LDD1251  [1]
 LDCM0373  CL104 PaTu 8988t C776(0.89); C782(0.89)  LDD1252  [1]
 LDCM0374  CL105 HCT 116 C776(0.74); C782(0.74)  LDD0691  [1]
 LDCM0375  CL106 HCT 116 C776(0.68); C782(0.68)  LDD0692  [1]
 LDCM0376  CL107 HCT 116 C776(0.79); C782(0.79)  LDD0693  [1]
 LDCM0377  CL108 HCT 116 C776(0.73); C782(0.73)  LDD0694  [1]
 LDCM0378  CL109 HCT 116 C776(0.76); C782(0.76)  LDD0695  [1]
 LDCM0379  CL11 HCT 116 C776(0.75); C782(0.75)  LDD0696  [1]
 LDCM0380  CL110 HCT 116 C776(0.85); C782(0.85)  LDD0697  [1]
 LDCM0381  CL111 HCT 116 C776(0.91); C782(0.91)  LDD0698  [1]
 LDCM0387  CL117 HCT 116 C776(0.98); C782(0.98)  LDD0704  [1]
 LDCM0388  CL118 HCT 116 C776(0.92); C782(0.92)  LDD0705  [1]
 LDCM0389  CL119 HCT 116 C776(0.96); C782(0.96)  LDD0706  [1]
 LDCM0390  CL12 HCT 116 C776(0.86); C782(0.86)  LDD0707  [1]
 LDCM0391  CL120 HCT 116 C776(1.07); C782(1.07)  LDD0708  [1]
 LDCM0396  CL125 HCT 116 C776(1.12); C782(1.12)  LDD0713  [1]
 LDCM0397  CL126 HCT 116 C776(0.95); C782(0.95)  LDD0714  [1]
 LDCM0398  CL127 HCT 116 C776(0.87); C782(0.87)  LDD0715  [1]
 LDCM0399  CL128 HCT 116 C776(0.77); C782(0.77)  LDD0716  [1]
 LDCM0400  CL13 HCT 116 C776(0.82); C782(0.82)  LDD0717  [1]
 LDCM0401  CL14 HCT 116 C776(0.85); C782(0.85)  LDD0718  [1]
 LDCM0402  CL15 HCT 116 C776(0.88); C782(0.88)  LDD0719  [1]
 LDCM0403  CL16 HCT 116 C776(1.13); C782(1.13)  LDD0720  [1]
 LDCM0404  CL17 HCT 116 C776(0.78); C782(0.78)  LDD0721  [1]
 LDCM0405  CL18 HCT 116 C776(0.77); C782(0.77)  LDD0722  [1]
 LDCM0406  CL19 HCT 116 C776(1.17); C782(1.17)  LDD0723  [1]
 LDCM0407  CL2 HCT 116 C776(1.01); C782(1.01)  LDD0724  [1]
 LDCM0408  CL20 HCT 116 C776(1.08); C782(1.08)  LDD0725  [1]
 LDCM0409  CL21 HCT 116 C776(0.86); C782(0.86)  LDD0726  [1]
 LDCM0410  CL22 HCT 116 C776(1.07); C782(1.07)  LDD0727  [1]
 LDCM0411  CL23 HCT 116 C776(0.90); C782(0.90)  LDD0728  [1]
 LDCM0412  CL24 HCT 116 C776(1.05); C782(1.05)  LDD0729  [1]
 LDCM0413  CL25 HCT 116 C776(0.89); C782(0.89)  LDD0730  [1]
 LDCM0414  CL26 HCT 116 C776(0.98); C782(0.98)  LDD0731  [1]
 LDCM0415  CL27 HCT 116 C776(0.92); C782(0.92)  LDD0732  [1]
 LDCM0416  CL28 HCT 116 C776(0.83); C782(0.83)  LDD0733  [1]
 LDCM0417  CL29 HCT 116 C776(0.93); C782(0.93)  LDD0734  [1]
 LDCM0418  CL3 HCT 116 C776(0.97); C782(0.97)  LDD0735  [1]
 LDCM0419  CL30 HCT 116 C776(0.99); C782(0.99)  LDD0736  [1]
 LDCM0429  CL4 HCT 116 C776(0.88); C782(0.88)  LDD0746  [1]
 LDCM0440  CL5 HCT 116 C776(0.88); C782(0.88)  LDD0757  [1]
 LDCM0451  CL6 HCT 116 C776(0.78); C782(0.78)  LDD0768  [1]
 LDCM0462  CL7 HCT 116 C776(0.98); C782(0.98)  LDD0779  [1]
 LDCM0473  CL8 HCT 116 C776(0.95); C782(0.95)  LDD0790  [1]
 LDCM0484  CL9 HCT 116 C776(0.87); C782(0.87)  LDD0801  [1]
 LDCM0486  CL91 HCT 116 C776(0.93); C782(0.93)  LDD0803  [1]
 LDCM0487  CL92 HCT 116 C776(0.85); C782(0.85)  LDD0804  [1]
 LDCM0488  CL93 HCT 116 C776(0.77); C782(0.77)  LDD0805  [1]
 LDCM0489  CL94 HCT 116 C776(0.86); C782(0.86)  LDD0806  [1]
 LDCM0490  CL95 HCT 116 C776(1.03); C782(1.03)  LDD0807  [1]
 LDCM0491  CL96 HCT 116 C776(1.05); C782(1.05)  LDD0808  [1]
 LDCM0492  CL97 HCT 116 C776(0.88); C782(0.88)  LDD0809  [1]
 LDCM0493  CL98 HCT 116 C776(0.76); C782(0.76)  LDD0810  [1]
 LDCM0494  CL99 HCT 116 C776(0.81); C782(0.81)  LDD0811  [1]
 LDCM0022  KB02 HEK-293T C498(0.99)  LDD1492  [4]
 LDCM0023  KB03 HEK-293T C498(1.02)  LDD1497  [4]
 LDCM0024  KB05 HEK-293T C498(0.83)  LDD1502  [4]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Transcription factor
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Transcription factor E2F1 (E2F1) E2F/DP family Q01094
Other
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Microcephalin (MCPH1) . Q8NEM0
Telomeric repeat-binding factor 2 (TERF2) . Q15554

References

1 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
2 Cyclopropenone, Cyclopropeniminium Ion, and Cyclopropenethione as Novel Electrophilic Warheads for Potential Target Discovery of Triple-Negative Breast Cancer. J Med Chem. 2023 Feb 23;66(4):2851-2864. doi: 10.1021/acs.jmedchem.2c01889. Epub 2023 Feb 10.
3 Benchmarking Cleavable Biotin Tags for Peptide-Centric Chemoproteomics. J Proteome Res. 2022 May 6;21(5):1349-1358. doi: 10.1021/acs.jproteome.2c00174. Epub 2022 Apr 25.
Mass spectrometry data entry: PXD031019
4 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402