Details of the Target

General Information of Target

Target ID LDTP09058
Target Name Formylglycine-generating enzyme (SUMF1)
Gene Name SUMF1
Gene ID 285362
Synonyms
Formylglycine-generating enzyme; FGE; EC 1.8.3.7; C-alpha-formylglycine-generating enzyme 1; Sulfatase-modifying factor 1
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MAAPALGLVCGRCPELGLVLLLLLLSLLCGAAGSQEAGTGAGAGSLAGSCGCGTPQRPGA
HGSSAAAHRYSREANAPGPVPGERQLAHSKMVPIPAGVFTMGTDDPQIKQDGEAPARRVT
IDAFYMDAYEVSNTEFEKFVNSTGYLTEAEKFGDSFVFEGMLSEQVKTNIQQAVAAAPWW
LPVKGANWRHPEGPDSTILHRPDHPVLHVSWNDAVAYCTWAGKRLPTEAEWEYSCRGGLH
NRLFPWGNKLQPKGQHYANIWQGEFPVTNTGEDGFQGTAPVDAFPPNGYGLYNIVGNAWE
WTSDWWTVHHSVEETLNPKGPPSGKDRVKKGGSYMCHRSYCYRYRCAARSQNTPDSSASN
LGFRCAADRLPTMD
Target Bioclass
Enzyme
Family
Sulfatase-modifying factor family
Subcellular location
Endoplasmic reticulum lumen
Function
Oxidase that catalyzes the conversion of cysteine to 3-oxoalanine on target proteins, using molecular oxygen and an unidentified reducing agent . 3-oxoalanine modification, which is also named formylglycine (fGly), occurs in the maturation of arylsulfatases and some alkaline phosphatases that use the hydrated form of 3-oxoalanine as a catalytic nucleophile. Known substrates include GALNS, ARSA, STS and ARSE.
Uniprot ID
Q8NBK3
Ensemble ID
ENST00000272902.10
HGNC ID
HGNC:20376

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 5 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
STPyne
 Probe Info  		K249(10.00)  LDD0277  [1]
IPM
 Probe Info  		N.A.  LDD0241  [2]
DBIA
 Probe Info  		C336(1.28)  LDD3310  [3]
DA-P3
 Probe Info  		4.66  LDD0179  [4]
IA-alkyne
 Probe Info  		N.A.  LDD0165  [5]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0226  AC11 HEK-293T C336(1.03)  LDD1509  [6]
 LDCM0237  AC12 HEK-293T C336(1.00)  LDD1510  [6]
 LDCM0259  AC14 HEK-293T C336(1.00)  LDD1512  [6]
 LDCM0278  AC19 HEK-293T C336(0.80)  LDD1517  [6]
 LDCM0280  AC20 HEK-293T C336(0.97)  LDD1519  [6]
 LDCM0282  AC22 HEK-293T C336(1.05)  LDD1521  [6]
 LDCM0284  AC24 HEK-293T C336(1.02)  LDD1523  [6]
 LDCM0287  AC27 HEK-293T C336(0.89)  LDD1526  [6]
 LDCM0288  AC28 HEK-293T C336(1.03)  LDD1527  [6]
 LDCM0290  AC3 HEK-293T C336(0.89)  LDD1529  [6]
 LDCM0291  AC30 HEK-293T C336(0.97)  LDD1530  [6]
 LDCM0293  AC32 HEK-293T C336(1.11)  LDD1532  [6]
 LDCM0296  AC35 HEK-293T C336(0.91)  LDD1535  [6]
 LDCM0297  AC36 HEK-293T C336(0.99)  LDD1536  [6]
 LDCM0299  AC38 HEK-293T C336(1.00)  LDD1538  [6]
 LDCM0301  AC4 HEK-293T C336(0.92)  LDD1540  [6]
 LDCM0302  AC40 HEK-293T C336(1.04)  LDD1541  [6]
 LDCM0305  AC43 HEK-293T C336(1.05)  LDD1544  [6]
 LDCM0306  AC44 HEK-293T C336(0.98)  LDD1545  [6]
 LDCM0308  AC46 HEK-293T C336(1.04)  LDD1547  [6]
 LDCM0310  AC48 HEK-293T C336(1.10)  LDD1549  [6]
 LDCM0314  AC51 HEK-293T C336(0.91)  LDD1553  [6]
 LDCM0315  AC52 HEK-293T C336(1.03)  LDD1554  [6]
 LDCM0317  AC54 HEK-293T C336(1.04)  LDD1556  [6]
 LDCM0319  AC56 HEK-293T C336(1.03)  LDD1558  [6]
 LDCM0322  AC59 HEK-293T C336(0.96)  LDD1561  [6]
 LDCM0323  AC6 HEK-293T C336(0.97)  LDD1562  [6]
 LDCM0324  AC60 HEK-293T C336(1.11)  LDD1563  [6]
 LDCM0326  AC62 HEK-293T C336(0.99)  LDD1565  [6]
 LDCM0328  AC64 HEK-293T C336(1.17)  LDD1567  [6]
 LDCM0345  AC8 HEK-293T C336(1.17)  LDD1569  [6]
 LDCM0275  AKOS034007705 HEK-293T C336(1.06)  LDD1514  [6]
 LDCM0368  CL10 HEK-293T C336(0.77)  LDD1572  [6]
 LDCM0369  CL100 HEK-293T C336(1.22)  LDD1573  [6]
 LDCM0372  CL103 HEK-293T C336(1.34)  LDD1576  [6]
 LDCM0373  CL104 HEK-293T C336(0.91)  LDD1577  [6]
 LDCM0376  CL107 HEK-293T C336(1.11)  LDD1580  [6]
 LDCM0377  CL108 HEK-293T C336(0.95)  LDD1581  [6]
 LDCM0381  CL111 HEK-293T C336(1.02)  LDD1585  [6]
 LDCM0382  CL112 HEK-293T C336(1.01)  LDD1586  [6]
 LDCM0385  CL115 HEK-293T C336(1.13)  LDD1589  [6]
 LDCM0386  CL116 HEK-293T C336(1.21)  LDD1590  [6]
 LDCM0389  CL119 HEK-293T C336(1.22)  LDD1593  [6]
 LDCM0390  CL12 HEK-293T C336(1.05)  LDD1594  [6]
 LDCM0391  CL120 HEK-293T C336(1.18)  LDD1595  [6]
 LDCM0394  CL123 HEK-293T C336(0.94)  LDD1598  [6]
 LDCM0395  CL124 HEK-293T C336(0.90)  LDD1599  [6]
 LDCM0398  CL127 HEK-293T C336(0.90)  LDD1602  [6]
 LDCM0399  CL128 HEK-293T C336(1.04)  LDD1603  [6]
 LDCM0402  CL15 HEK-293T C336(1.04)  LDD1606  [6]
 LDCM0403  CL16 HEK-293T C336(0.90)  LDD1607  [6]
 LDCM0406  CL19 HEK-293T C336(0.84)  LDD1610  [6]
 LDCM0408  CL20 HEK-293T C336(1.32)  LDD1612  [6]
 LDCM0410  CL22 HEK-293T C336(0.97)  LDD1614  [6]
 LDCM0412  CL24 HEK-293T C336(1.04)  LDD1616  [6]
 LDCM0415  CL27 HEK-293T C336(1.19)  LDD1619  [6]
 LDCM0416  CL28 HEK-293T C336(1.10)  LDD1620  [6]
 LDCM0418  CL3 HEK-293T C336(0.99)  LDD1622  [6]
 LDCM0420  CL31 HEK-293T C336(0.90)  LDD1624  [6]
 LDCM0421  CL32 HEK-293T C336(0.99)  LDD1625  [6]
 LDCM0423  CL34 HEK-293T C336(0.95)  LDD1627  [6]
 LDCM0425  CL36 HEK-293T C336(1.02)  LDD1629  [6]
 LDCM0428  CL39 HEK-293T C336(1.18)  LDD1632  [6]
 LDCM0429  CL4 HEK-293T C336(0.80)  LDD1633  [6]
 LDCM0430  CL40 HEK-293T C336(0.91)  LDD1634  [6]
 LDCM0433  CL43 HEK-293T C336(0.93)  LDD1637  [6]
 LDCM0434  CL44 HEK-293T C336(1.00)  LDD1638  [6]
 LDCM0436  CL46 HEK-293T C336(0.96)  LDD1640  [6]
 LDCM0438  CL48 HEK-293T C336(1.19)  LDD1642  [6]
 LDCM0443  CL52 HEK-293T C336(1.15)  LDD1646  [6]
 LDCM0446  CL55 HEK-293T C336(1.00)  LDD1649  [6]
 LDCM0447  CL56 HEK-293T C336(1.05)  LDD1650  [6]
 LDCM0449  CL58 HEK-293T C336(0.98)  LDD1652  [6]
 LDCM0452  CL60 HEK-293T C336(0.98)  LDD1655  [6]
 LDCM0455  CL63 HEK-293T C336(1.14)  LDD1658  [6]
 LDCM0456  CL64 HEK-293T C336(1.12)  LDD1659  [6]
 LDCM0459  CL67 HEK-293T C336(1.01)  LDD1662  [6]
 LDCM0460  CL68 HEK-293T C336(1.04)  LDD1663  [6]
 LDCM0462  CL7 HEK-293T C336(1.00)  LDD1665  [6]
 LDCM0463  CL70 HEK-293T C336(0.94)  LDD1666  [6]
 LDCM0465  CL72 HEK-293T C336(1.10)  LDD1668  [6]
 LDCM0469  CL76 HEK-293T C336(0.87)  LDD1672  [6]
 LDCM0472  CL79 HEK-293T C336(0.83)  LDD1675  [6]
 LDCM0473  CL8 HEK-293T C336(0.63)  LDD1676  [6]
 LDCM0474  CL80 HEK-293T C336(1.12)  LDD1677  [6]
 LDCM0476  CL82 HEK-293T C336(0.99)  LDD1679  [6]
 LDCM0478  CL84 HEK-293T C336(1.07)  LDD1681  [6]
 LDCM0481  CL87 HEK-293T C336(0.86)  LDD1684  [6]
 LDCM0482  CL88 HEK-293T C336(0.72)  LDD1685  [6]
 LDCM0486  CL91 HEK-293T C336(0.95)  LDD1689  [6]
 LDCM0487  CL92 HEK-293T C336(0.83)  LDD1690  [6]
 LDCM0489  CL94 HEK-293T C336(0.98)  LDD1692  [6]
 LDCM0491  CL96 HEK-293T C336(1.15)  LDD1694  [6]
 LDCM0494  CL99 HEK-293T C336(1.07)  LDD1697  [6]
 LDCM0027  Dopamine HEK-293T 4.66  LDD0179  [4]
 LDCM0495  E2913 HEK-293T C336(1.00)  LDD1698  [6]
 LDCM0468  Fragment33 HEK-293T C336(0.87)  LDD1671  [6]
 LDCM0022  KB02 HEK-293T C336(1.00)  LDD1492  [6]
 LDCM0023  KB03 HEK-293T C336(0.95)  LDD1497  [6]
 LDCM0024  KB05 COLO792 C336(1.28)  LDD3310  [3]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 3 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
N-sulphoglucosamine sulphohydrolase (SGSH) Sulfatase family P51688
Formylglycine-generating enzyme (SUMF1) Sulfatase-modifying factor family Q8NBK3
Inactive C-alpha-formylglycine-generating enzyme 2 (SUMF2) Sulfatase-modifying factor family Q8NBJ7

References

1 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
2 Oxidant-Induced Bioconjugation for Protein Labeling in Live Cells. ACS Chem Biol. 2023 Jan 20;18(1):112-122. doi: 10.1021/acschembio.2c00740. Epub 2022 Dec 21.
3 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
4 A chemical probe unravels the reactive proteome of health-associated catechols. Chem Sci. 2023 Jul 22;14(32):8635-8643. doi: 10.1039/d3sc00888f. eCollection 2023 Aug 16.
Mass spectrometry data entry: PXD043348
5 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
6 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402