Details of the Target

General Information of Target

Target ID LDTP08705
Target Name Mucolipin-2 (MCOLN2)
Gene Name MCOLN2
Gene ID 255231
Synonyms
Mucolipin-2; Transient receptor potential channel mucolipin 2; TRPML2
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MARQPYRFPQARIPERGSGVFRLTVRNAMAHRDSEMKEECLREDLKFYFMSPCEKYRARR
QIPWKLGLQILKIVMVTTQLVRFGLSNQLVVAFKEDNTVAFKHLFLKGYSGTDEDDYSCS
VYTQEDAYESIFFAINQYHQLKDITLGTLGYGENEDNRIGLKVCKQHYKKGTMFPSNETL
NIDNDVELDCVQLDLQDLSKKPPDWKNSSFFRLEFYRLLQVEISFHLKGIDLQTIHSREL
PDCYVFQNTIIFDNKAHSGKIKIYFDSDAKIEECKDLNIFGSTQKNAQYVLVFDAFVIVI
CLASLILCTRSIVLALRLRKRFLNFFLEKYKRPVCDTDQWEFINGWYVLVIISDLMTIIG
SILKMEIKAKNLTNYDLCSIFLGTSTLLVWVGVIRYLGYFQAYNVLILTMQASLPKVLRF
CACAGMIYLGYTFCGWIVLGPYHDKFENLNTVAECLFSLVNGDDMFATFAQIQQKSILVW
LFSRLYLYSFISLFIYMILSLFIALITDSYDTIKKFQQNGFPETDLQEFLKECSSKEEYQ
KESSAFLSCICCRRRKRSDDHLIPIS
Target Bioclass
Transporter and channel
Family
Transient receptor (TC 1.A.4) family, Polycystin subfamily, MCOLN2 sub-subfamily
Subcellular location
Cell membrane
Function
Nonselective cation channel probably playing a role in the regulation of membrane trafficking events. Acts as a Ca(2+)-permeable cation channel with inwardly rectifying activity. May activate ARF6 and be involved in the trafficking of GPI-anchored cargo proteins to the cell surface via the ARF6-regulated recycling pathway. May play a role in immune processes. In adaptive immunity, TRPML2 and TRPML1 may play redundant roles in the function of the specialized lysosomes of B cells. In the innate immune response, may play a role in the regulation of chemokine secretion and macrophage migration. Through a possible and probably tissue-specific heteromerization with MCOLN1 may be at least in part involved in many lysosome-dependent cellular events.
Uniprot ID
Q8IZK6
Ensemble ID
ENST00000284027.5
HGNC ID
HGNC:13357
ChEMBL ID
CHEMBL4879493

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
22RV1 SNV: p.S258I .
LN18 SNV: p.D126H .
NCIH1155 SNV: p.V299M .
NCIH2286 SNV: p.V389L .
OVCAR4 SNV: p.D183E .
PF382 Deletion: p.M365WfsTer42
SNV: p.A30T		 .
SAOS2 SNV: p.T145I .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 1 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C53(3.02)  LDD3427  [1]
PAL-AfBPP Probe
Click To Hide/Show 2 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
STS-1
 Probe Info  		N.A.  LDD0136  [2]
STS-2
 Probe Info  		N.A.  LDD0138  [2]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0214  AC1 HEK-293T C53(0.78)  LDD1507  [3]
 LDCM0215  AC10 HEK-293T C53(1.24)  LDD1508  [3]
 LDCM0226  AC11 HEK-293T C53(0.99)  LDD1509  [3]
 LDCM0237  AC12 HEK-293T C53(1.17)  LDD1510  [3]
 LDCM0259  AC14 HEK-293T C53(1.05)  LDD1512  [3]
 LDCM0270  AC15 HEK-293T C53(0.98); C40(0.77)  LDD1513  [3]
 LDCM0276  AC17 HEK-293T C53(1.10)  LDD1515  [3]
 LDCM0277  AC18 HEK-293T C53(1.59)  LDD1516  [3]
 LDCM0278  AC19 HEK-293T C53(1.21)  LDD1517  [3]
 LDCM0279  AC2 HEK-293T C53(0.89)  LDD1518  [3]
 LDCM0280  AC20 HEK-293T C53(1.00)  LDD1519  [3]
 LDCM0281  AC21 HEK-293T C53(0.99); C40(0.86)  LDD1520  [3]
 LDCM0282  AC22 HEK-293T C53(1.04)  LDD1521  [3]
 LDCM0283  AC23 HEK-293T C53(0.84); C40(0.79)  LDD1522  [3]
 LDCM0284  AC24 HEK-293T C53(0.85)  LDD1523  [3]
 LDCM0285  AC25 HEK-293T C53(0.95)  LDD1524  [3]
 LDCM0286  AC26 HEK-293T C53(1.04)  LDD1525  [3]
 LDCM0287  AC27 HEK-293T C53(0.85)  LDD1526  [3]
 LDCM0288  AC28 HEK-293T C53(0.71)  LDD1527  [3]
 LDCM0289  AC29 HEK-293T C53(0.99); C40(0.85)  LDD1528  [3]
 LDCM0290  AC3 HEK-293T C53(0.77)  LDD1529  [3]
 LDCM0291  AC30 HEK-293T C53(1.15)  LDD1530  [3]
 LDCM0292  AC31 HEK-293T C53(1.00); C40(0.84)  LDD1531  [3]
 LDCM0293  AC32 HEK-293T C53(0.94)  LDD1532  [3]
 LDCM0294  AC33 HEK-293T C53(0.82)  LDD1533  [3]
 LDCM0295  AC34 HEK-293T C53(0.78)  LDD1534  [3]
 LDCM0296  AC35 HEK-293T C53(0.86)  LDD1535  [3]
 LDCM0297  AC36 HEK-293T C53(0.81)  LDD1536  [3]
 LDCM0298  AC37 HEK-293T C53(1.07); C40(0.84)  LDD1537  [3]
 LDCM0299  AC38 HEK-293T C53(1.12)  LDD1538  [3]
 LDCM0300  AC39 HEK-293T C53(0.85); C40(0.82)  LDD1539  [3]
 LDCM0301  AC4 HEK-293T C53(0.80)  LDD1540  [3]
 LDCM0302  AC40 HEK-293T C53(0.92)  LDD1541  [3]
 LDCM0303  AC41 HEK-293T C53(0.86)  LDD1542  [3]
 LDCM0304  AC42 HEK-293T C53(0.92)  LDD1543  [3]
 LDCM0305  AC43 HEK-293T C53(0.75)  LDD1544  [3]
 LDCM0306  AC44 HEK-293T C53(0.94)  LDD1545  [3]
 LDCM0307  AC45 HEK-293T C53(1.40); C40(0.90)  LDD1546  [3]
 LDCM0308  AC46 HEK-293T C53(1.39)  LDD1547  [3]
 LDCM0309  AC47 HEK-293T C53(0.87); C40(1.13)  LDD1548  [3]
 LDCM0310  AC48 HEK-293T C53(0.92)  LDD1549  [3]
 LDCM0311  AC49 HEK-293T C53(1.04)  LDD1550  [3]
 LDCM0312  AC5 HEK-293T C53(1.13); C40(1.07)  LDD1551  [3]
 LDCM0313  AC50 HEK-293T C53(1.30)  LDD1552  [3]
 LDCM0314  AC51 HEK-293T C53(1.01)  LDD1553  [3]
 LDCM0315  AC52 HEK-293T C53(0.87)  LDD1554  [3]
 LDCM0316  AC53 HEK-293T C53(1.07); C40(0.95)  LDD1555  [3]
 LDCM0317  AC54 HEK-293T C53(1.31)  LDD1556  [3]
 LDCM0318  AC55 HEK-293T C53(0.70); C40(0.88)  LDD1557  [3]
 LDCM0319  AC56 HEK-293T C53(0.96)  LDD1558  [3]
 LDCM0320  AC57 HEK-293T C53(1.06)  LDD1559  [3]
 LDCM0321  AC58 HEK-293T C53(0.97)  LDD1560  [3]
 LDCM0322  AC59 HEK-293T C53(0.93)  LDD1561  [3]
 LDCM0323  AC6 HEK-293T C53(1.55)  LDD1562  [3]
 LDCM0324  AC60 HEK-293T C53(0.88)  LDD1563  [3]
 LDCM0325  AC61 HEK-293T C53(1.09); C40(1.16)  LDD1564  [3]
 LDCM0326  AC62 HEK-293T C53(1.04)  LDD1565  [3]
 LDCM0327  AC63 HEK-293T C53(0.91); C40(1.05)  LDD1566  [3]
 LDCM0328  AC64 HEK-293T C53(1.08)  LDD1567  [3]
 LDCM0334  AC7 HEK-293T C53(0.86); C40(0.96)  LDD1568  [3]
 LDCM0345  AC8 HEK-293T C53(1.03)  LDD1569  [3]
 LDCM0248  AKOS034007472 HEK-293T C53(1.09); C40(0.83)  LDD1511  [3]
 LDCM0356  AKOS034007680 HEK-293T C53(0.83)  LDD1570  [3]
 LDCM0275  AKOS034007705 HEK-293T C53(1.16)  LDD1514  [3]
 LDCM0367  CL1 HEK-293T C53(1.04)  LDD1571  [3]
 LDCM0368  CL10 HEK-293T C53(1.88)  LDD1572  [3]
 LDCM0369  CL100 HEK-293T C53(1.30)  LDD1573  [3]
 LDCM0370  CL101 HEK-293T C53(0.83)  LDD1574  [3]
 LDCM0373  CL104 HEK-293T C53(1.26)  LDD1577  [3]
 LDCM0374  CL105 HEK-293T C53(1.04)  LDD1578  [3]
 LDCM0377  CL108 HEK-293T C53(1.41)  LDD1581  [3]
 LDCM0378  CL109 HEK-293T C53(1.08)  LDD1582  [3]
 LDCM0379  CL11 HEK-293T C53(1.35); C40(0.39)  LDD1583  [3]
 LDCM0382  CL112 HEK-293T C53(1.03)  LDD1586  [3]
 LDCM0383  CL113 HEK-293T C53(0.88)  LDD1587  [3]
 LDCM0386  CL116 HEK-293T C53(0.90)  LDD1590  [3]
 LDCM0387  CL117 HEK-293T C53(0.85)  LDD1591  [3]
 LDCM0390  CL12 HEK-293T C53(1.28)  LDD1594  [3]
 LDCM0391  CL120 HEK-293T C53(0.81)  LDD1595  [3]
 LDCM0392  CL121 HEK-293T C53(0.80)  LDD1596  [3]
 LDCM0395  CL124 HEK-293T C53(1.71)  LDD1599  [3]
 LDCM0396  CL125 HEK-293T C53(0.80)  LDD1600  [3]
 LDCM0399  CL128 HEK-293T C53(1.24)  LDD1603  [3]
 LDCM0400  CL13 HEK-293T C53(0.79)  LDD1604  [3]
 LDCM0403  CL16 HEK-293T C53(0.99)  LDD1607  [3]
 LDCM0404  CL17 HEK-293T C53(1.28)  LDD1608  [3]
 LDCM0405  CL18 HEK-293T C53(1.19)  LDD1609  [3]
 LDCM0406  CL19 HEK-293T C53(1.12)  LDD1610  [3]
 LDCM0408  CL20 HEK-293T C53(0.97)  LDD1612  [3]
 LDCM0409  CL21 HEK-293T C53(1.35); C40(0.42)  LDD1613  [3]
 LDCM0410  CL22 HEK-293T C53(1.28)  LDD1614  [3]
 LDCM0411  CL23 HEK-293T C53(1.05); C40(0.40)  LDD1615  [3]
 LDCM0412  CL24 HEK-293T C53(0.95)  LDD1616  [3]
 LDCM0413  CL25 HEK-293T C53(1.25)  LDD1617  [3]
 LDCM0416  CL28 HEK-293T C53(0.90)  LDD1620  [3]
 LDCM0417  CL29 HEK-293T C53(1.08)  LDD1621  [3]
 LDCM0419  CL30 HEK-293T C53(1.05)  LDD1623  [3]
 LDCM0420  CL31 HEK-293T C53(1.09)  LDD1624  [3]
 LDCM0421  CL32 HEK-293T C53(1.27)  LDD1625  [3]
 LDCM0422  CL33 HEK-293T C53(2.33); C40(0.41)  LDD1626  [3]
 LDCM0423  CL34 HEK-293T C53(1.43)  LDD1627  [3]
 LDCM0424  CL35 HEK-293T C53(0.67); C40(0.40)  LDD1628  [3]
 LDCM0425  CL36 HEK-293T C53(0.69)  LDD1629  [3]
 LDCM0426  CL37 HEK-293T C53(1.31)  LDD1630  [3]
 LDCM0429  CL4 HEK-293T C53(1.30)  LDD1633  [3]
 LDCM0430  CL40 HEK-293T C53(0.89)  LDD1634  [3]
 LDCM0431  CL41 HEK-293T C53(0.88)  LDD1635  [3]
 LDCM0432  CL42 HEK-293T C53(0.83)  LDD1636  [3]
 LDCM0433  CL43 HEK-293T C53(0.81)  LDD1637  [3]
 LDCM0434  CL44 HEK-293T C53(1.06)  LDD1638  [3]
 LDCM0435  CL45 HEK-293T C53(1.63); C40(0.44)  LDD1639  [3]
 LDCM0436  CL46 HEK-293T C53(1.02)  LDD1640  [3]
 LDCM0437  CL47 HEK-293T C53(1.33); C40(0.36)  LDD1641  [3]
 LDCM0438  CL48 HEK-293T C53(1.00)  LDD1642  [3]
 LDCM0439  CL49 HEK-293T C53(0.99)  LDD1643  [3]
 LDCM0440  CL5 HEK-293T C53(0.80)  LDD1644  [3]
 LDCM0443  CL52 HEK-293T C53(0.80)  LDD1646  [3]
 LDCM0444  CL53 HEK-293T C53(0.91)  LDD1647  [3]
 LDCM0445  CL54 HEK-293T C53(1.03)  LDD1648  [3]
 LDCM0446  CL55 HEK-293T C53(0.74)  LDD1649  [3]
 LDCM0447  CL56 HEK-293T C53(1.06)  LDD1650  [3]
 LDCM0448  CL57 HEK-293T C53(1.59); C40(0.42)  LDD1651  [3]
 LDCM0449  CL58 HEK-293T C53(1.86)  LDD1652  [3]
 LDCM0450  CL59 HEK-293T C53(0.83); C40(0.44)  LDD1653  [3]
 LDCM0451  CL6 HEK-293T C53(1.59)  LDD1654  [3]
 LDCM0452  CL60 HEK-293T C53(1.19)  LDD1655  [3]
 LDCM0453  CL61 HEK-293T C53(0.78)  LDD1656  [3]
 LDCM0456  CL64 HEK-293T C53(1.80)  LDD1659  [3]
 LDCM0457  CL65 HEK-293T C53(0.87)  LDD1660  [3]
 LDCM0458  CL66 HEK-293T C53(0.60)  LDD1661  [3]
 LDCM0459  CL67 HEK-293T C53(0.73)  LDD1662  [3]
 LDCM0460  CL68 HEK-293T C53(1.24)  LDD1663  [3]
 LDCM0461  CL69 HEK-293T C53(1.36); C40(0.47)  LDD1664  [3]
 LDCM0462  CL7 HEK-293T C53(1.10)  LDD1665  [3]
 LDCM0463  CL70 HEK-293T C53(1.78)  LDD1666  [3]
 LDCM0464  CL71 HEK-293T C53(1.02); C40(0.37)  LDD1667  [3]
 LDCM0465  CL72 HEK-293T C53(1.28)  LDD1668  [3]
 LDCM0466  CL73 HEK-293T C53(0.76)  LDD1669  [3]
 LDCM0469  CL76 HEK-293T C53(0.71)  LDD1672  [3]
 LDCM0470  CL77 HEK-293T C53(1.16)  LDD1673  [3]
 LDCM0471  CL78 HEK-293T C53(0.92)  LDD1674  [3]
 LDCM0472  CL79 HEK-293T C53(1.17)  LDD1675  [3]
 LDCM0473  CL8 HEK-293T C53(2.08)  LDD1676  [3]
 LDCM0474  CL80 HEK-293T C53(1.06)  LDD1677  [3]
 LDCM0475  CL81 HEK-293T C53(1.46); C40(0.49)  LDD1678  [3]
 LDCM0476  CL82 HEK-293T C53(1.26)  LDD1679  [3]
 LDCM0477  CL83 HEK-293T C53(1.32); C40(0.43)  LDD1680  [3]
 LDCM0478  CL84 HEK-293T C53(1.55)  LDD1681  [3]
 LDCM0479  CL85 HEK-293T C53(0.70)  LDD1682  [3]
 LDCM0482  CL88 HEK-293T C53(1.17)  LDD1685  [3]
 LDCM0483  CL89 HEK-293T C53(0.75)  LDD1686  [3]
 LDCM0484  CL9 HEK-293T C53(1.57); C40(0.42)  LDD1687  [3]
 LDCM0485  CL90 HEK-293T C53(0.80)  LDD1688  [3]
 LDCM0486  CL91 HEK-293T C53(0.78)  LDD1689  [3]
 LDCM0487  CL92 HEK-293T C53(0.86)  LDD1690  [3]
 LDCM0488  CL93 HEK-293T C53(1.58); C40(0.55)  LDD1691  [3]
 LDCM0489  CL94 HEK-293T C53(1.41)  LDD1692  [3]
 LDCM0490  CL95 HEK-293T C53(1.61); C40(0.49)  LDD1693  [3]
 LDCM0491  CL96 HEK-293T C53(0.85)  LDD1694  [3]
 LDCM0492  CL97 HEK-293T C53(0.85)  LDD1695  [3]
 LDCM0022  KB02 A2058 C53(2.37)  LDD2253  [1]
 LDCM0023  KB03 A2058 C53(3.63)  LDD2670  [1]
 LDCM0024  KB05 SK-MEL-28 C53(3.02)  LDD3427  [1]

References

1 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
2 Design and synthesis of minimalist terminal alkyne-containing diazirine photo-crosslinkers and their incorporation into kinase inhibitors for cell- and tissue-based proteome profiling. Angew Chem Int Ed Engl. 2013 Aug 12;52(33):8551-6. doi: 10.1002/anie.201300683. Epub 2013 Jun 10.
3 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402