Details of the Target

General Information of Target

Target ID LDTP08619
Target Name Probable ATP-dependent RNA helicase DDX60 (DDX60)
Gene Name DDX60
Gene ID 55601
Synonyms
Probable ATP-dependent RNA helicase DDX60; EC 3.6.4.13; DEAD box protein 60
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MERNVLTTFSQEMSQLILNEMPKAEYSSLFNDFVESEFFLIDGDSLLITCICEISFKPGQ
NLHFFYLVERYLVDLISKGGQFTIVFFKDAEYAYFNFPELLSLRTALILHLQKNTTIDVR
TTFSRCLSKEWGSFLEESYPYFLIVADEGLNDLQTQLFNFLIIHSWARKVNVVLSSGQES
DVLCLYAYLLPSMYRHQIFSWKNKQNIKDAYTTLLNQLERFKLSALAPLFGSLKWNNITE
EAHKTVSLLTQVWPEGSDIRRVFCVTSCSLSLRMYHRFLGNREPSSGQETEIQQVNSNCL
TLQEMEDLCKLHCLTVVFLLHLPLSQRACARVITSHWAEDMKPLLQMKKWCEYFILRNIH
TFEFWNLNLIHLSDLNDELLLKNIAFYYENENVKGLHLNLGDTIMKDYEYLWNTVSKLVR
DFEVGQPFPLRTTKVCFLEKKPSPIKDSSNEMVPNLGFIPTSSFVVDKFAGDILKDLPFL
KSDDPIVTSLVKQKEFDELVHWHSHKPLSDDYDRSRCQFDEKSRDPRVLRSVQKYHVFQR
FYGNSLETVSSKIIVTQTIKSKKDFSGPKSKKAHETKAEIIARENKKRLFAREEQKEEQK
WNALSFSIEEQLKENLHSGIKSLEDFLKSCKSSCVKLQVEMVGLTACLKAWKEHCRSEEG
KTTKDLSIAVQVMKRIHSLMEKYSELLQEDDRQLIARCLKYLGFDELASSLHPAQDAEND
VKVKKRNKYSVGIGPARFQLQYMGHYLIRDERKDPDPRVQDFIPDTWQRELLDVVDKNES
AVIVAPTSSGKTYASYYCMEKVLKESDDGVVVYVAPTKALVNQVAATVQNRFTKNLPSGE
VLCGVFTREYRHDALNCQVLITVPACFEILLLAPHRQNWVKKIRYVIFDEVHCLGGEIGA
EIWEHLLVMIRCPFLALSATISNPEHLTEWLQSVKWYWKQEDKIIENNTASKRHVGRQAG
FPKDYLQVKQSYKVRLVLYGERYNDLEKHVCSIKHGDIHFDHFHPCAALTTDHIERYGFP
PDLTLSPRESIQLYDAMFQIWKSWPRAQELCPENFIHFNNKLVIKKMDARKYEESLKAEL
TSWIKNGNVEQARMVLQNLSPEADLSPENMITMFPLLVEKLRKMEKLPALFFLFKLGAVE
NAAESVSTFLKKKQETKRPPKADKEAHVMANKLRKVKKSIEKQKIIDEKSQKKTRNVDQS
LIHEAEHDNLVKCLEKNLEIPQDCTYADQKAVDTETLQKVFGRVKFERKGEELKALAERG
IGYHHSAMSFKEKQLVEILFRKGYLRVVTATGTLALGVNMPCKSVVFAQNSVYLDALNYR
QMSGRAGRRGQDLMGDVYFFDIPFPKIGKLIKSNVPELRGHFPLSITLVLRLMLLASKGD
DPEDAKAKVLSVLKHSLLSFKQPRVMDMLKLYFLFSLQFLVKEGYLDQEGNPMGFAGLVS
HLHYHEPSNLVFVSFLVNGLFHDLCQPTRKGSKHFSQDVMEKLVLVLAHLFGRRYFPPKF
QDAHFEFYQSKVFLDDLPEDFSDALDEYNMKIMEDFTTFLRIVSKLADMNQEYQLPLSKI
KFTGKECEDSQLVSHLMSCKEGRVAISPFVCLSGNFDDDLLRLETPNHVTLGTIGVNRSQ
APVLLSQKFDNRGRKMSLNAYALDFYKHGSLIGLVQDNRMNEGDAYYLLKDFALTIKSIS
VSLRELCENEDDNVVLAFEQLSTTFWEKLNKV
Target Bioclass
Enzyme
Family
Helicase family
Subcellular location
Cytoplasm
Function
Positively regulates RIGI- and IFIH1/MDA5-dependent type I interferon and interferon inducible gene expression in response to viral infection. Binds ssRNA, dsRNA and dsDNA and can promote the binding of RIGI to dsRNA. Exhibits antiviral activity against hepatitis C virus and vesicular stomatitis virus (VSV).
Uniprot ID
Q8IY21
Ensemble ID
ENST00000393743.8
HGNC ID
HGNC:25942

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 8 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
STPyne
 Probe Info  		K1239(0.51)  LDD0277  [1]
DBIA
 Probe Info  		C351(1.95); C843(1.49); C1591(1.27)  LDD3311  [2]
4-Iodoacetamidophenylacetylene
 Probe Info  		N.A.  LDD0038  [3]
IA-alkyne
 Probe Info  		N.A.  LDD0036  [3]
Lodoacetamide azide
 Probe Info  		N.A.  LDD0037  [3]
Compound 10
 Probe Info  		N.A.  LDD2216  [4]
Compound 11
 Probe Info  		N.A.  LDD2213  [4]
IPM
 Probe Info  		C1051(0.00); C517(0.00)  LDD0147  [5]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0214  AC1 PaTu 8988t C1567(0.30); C1579(0.30)  LDD1093  [6]
 LDCM0215  AC10 PaTu 8988t C1567(0.76); C1579(0.76)  LDD1094  [6]
 LDCM0226  AC11 PaTu 8988t C1567(1.24); C1579(1.24)  LDD1105  [6]
 LDCM0237  AC12 PaTu 8988t C1567(1.50); C1579(1.50)  LDD1116  [6]
 LDCM0246  AC128 PaTu 8988t C1567(1.14); C1579(1.14)  LDD1125  [6]
 LDCM0247  AC129 PaTu 8988t C1567(0.78); C1579(0.78)  LDD1126  [6]
 LDCM0249  AC130 PaTu 8988t C1567(0.89); C1579(0.89)  LDD1128  [6]
 LDCM0250  AC131 PaTu 8988t C1567(1.15); C1579(1.15)  LDD1129  [6]
 LDCM0251  AC132 PaTu 8988t C1567(0.87); C1579(0.87)  LDD1130  [6]
 LDCM0252  AC133 PaTu 8988t C1567(0.99); C1579(0.99)  LDD1131  [6]
 LDCM0253  AC134 PaTu 8988t C1567(1.40); C1579(1.40)  LDD1132  [6]
 LDCM0254  AC135 PaTu 8988t C1567(1.05); C1579(1.05)  LDD1133  [6]
 LDCM0255  AC136 PaTu 8988t C1567(1.03); C1579(1.03)  LDD1134  [6]
 LDCM0256  AC137 PaTu 8988t C1567(0.88); C1579(0.88)  LDD1135  [6]
 LDCM0257  AC138 PaTu 8988t C1567(1.37); C1579(1.37)  LDD1136  [6]
 LDCM0258  AC139 PaTu 8988t C1567(0.88); C1579(0.88)  LDD1137  [6]
 LDCM0259  AC14 PaTu 8988t C1567(0.95); C1579(0.95)  LDD1138  [6]
 LDCM0260  AC140 PaTu 8988t C1567(0.97); C1579(0.97)  LDD1139  [6]
 LDCM0261  AC141 PaTu 8988t C1567(0.79); C1579(0.79)  LDD1140  [6]
 LDCM0262  AC142 PaTu 8988t C1567(1.80); C1579(1.80)  LDD1141  [6]
 LDCM0270  AC15 PaTu 8988t C1567(1.02); C1579(1.02)  LDD1149  [6]
 LDCM0279  AC2 PaTu 8988t C1567(0.32); C1579(0.32)  LDD1158  [6]
 LDCM0285  AC25 PaTu 8988t C1567(0.89); C1579(0.89)  LDD1164  [6]
 LDCM0286  AC26 PaTu 8988t C1567(0.84); C1579(0.84)  LDD1165  [6]
 LDCM0287  AC27 PaTu 8988t C1567(1.06); C1579(1.06)  LDD1166  [6]
 LDCM0288  AC28 PaTu 8988t C1567(1.00); C1579(1.00)  LDD1167  [6]
 LDCM0289  AC29 PaTu 8988t C1567(1.39); C1579(1.39)  LDD1168  [6]
 LDCM0290  AC3 PaTu 8988t C1567(0.31); C1579(0.31)  LDD1169  [6]
 LDCM0291  AC30 PaTu 8988t C1567(1.03); C1579(1.03)  LDD1170  [6]
 LDCM0292  AC31 PaTu 8988t C1567(1.00); C1579(1.00)  LDD1171  [6]
 LDCM0293  AC32 PaTu 8988t C1567(1.24); C1579(1.24)  LDD1172  [6]
 LDCM0294  AC33 PaTu 8988t C1567(1.02); C1579(1.02)  LDD1173  [6]
 LDCM0295  AC34 PaTu 8988t C1567(1.13); C1579(1.13)  LDD1174  [6]
 LDCM0301  AC4 PaTu 8988t C1567(0.35); C1579(0.35)  LDD1180  [6]
 LDCM0312  AC5 PaTu 8988t C1567(0.30); C1579(0.30)  LDD1191  [6]
 LDCM0323  AC6 PaTu 8988t C1567(0.86); C1579(0.86)  LDD1202  [6]
 LDCM0334  AC7 PaTu 8988t C1567(1.06); C1579(1.06)  LDD1213  [6]
 LDCM0345  AC8 PaTu 8988t C1567(1.18); C1579(1.18)  LDD1224  [6]
 LDCM0349  AC83 PaTu 8988t C1567(0.62); C1579(0.62)  LDD1228  [6]
 LDCM0350  AC84 PaTu 8988t C1567(0.92); C1579(0.92)  LDD1229  [6]
 LDCM0351  AC85 PaTu 8988t C1567(0.70); C1579(0.70)  LDD1230  [6]
 LDCM0352  AC86 PaTu 8988t C1567(0.83); C1579(0.83)  LDD1231  [6]
 LDCM0353  AC87 PaTu 8988t C1567(0.87); C1579(0.87)  LDD1232  [6]
 LDCM0354  AC88 PaTu 8988t C1567(0.83); C1579(0.83)  LDD1233  [6]
 LDCM0355  AC89 PaTu 8988t C1567(0.94); C1579(0.94)  LDD1234  [6]
 LDCM0357  AC90 PaTu 8988t C1567(1.05); C1579(1.05)  LDD1236  [6]
 LDCM0358  AC91 PaTu 8988t C1567(0.99); C1579(0.99)  LDD1237  [6]
 LDCM0359  AC92 PaTu 8988t C1567(0.58); C1579(0.58)  LDD1238  [6]
 LDCM0360  AC93 PaTu 8988t C1567(0.69); C1579(0.69)  LDD1239  [6]
 LDCM0361  AC94 PaTu 8988t C1567(0.70); C1579(0.70)  LDD1240  [6]
 LDCM0362  AC95 PaTu 8988t C1567(0.79); C1579(0.79)  LDD1241  [6]
 LDCM0363  AC96 PaTu 8988t C1567(0.76); C1579(0.76)  LDD1242  [6]
 LDCM0364  AC97 PaTu 8988t C1567(0.68); C1579(0.68)  LDD1243  [6]
 LDCM0248  AKOS034007472 PaTu 8988t C1567(0.90); C1579(0.90)  LDD1127  [6]
 LDCM0356  AKOS034007680 PaTu 8988t C1567(0.93); C1579(0.93)  LDD1235  [6]
 LDCM0275  AKOS034007705 PaTu 8988t C1567(0.93); C1579(0.93)  LDD1154  [6]
 LDCM0369  CL100 PaTu 8988t C1567(0.26); C1579(0.26)  LDD1248  [6]
 LDCM0370  CL101 PaTu 8988t C1567(0.91); C1579(0.91)  LDD1249  [6]
 LDCM0371  CL102 PaTu 8988t C1567(1.72); C1579(1.72)  LDD1250  [6]
 LDCM0372  CL103 PaTu 8988t C1567(1.13); C1579(1.13)  LDD1251  [6]
 LDCM0373  CL104 PaTu 8988t C1567(1.28); C1579(1.28)  LDD1252  [6]
 LDCM0382  CL112 PaTu 8988t C1567(1.06); C1579(1.06)  LDD1261  [6]
 LDCM0383  CL113 PaTu 8988t C1567(1.23); C1579(1.23)  LDD1262  [6]
 LDCM0384  CL114 PaTu 8988t C1567(1.51); C1579(1.51)  LDD1263  [6]
 LDCM0385  CL115 PaTu 8988t C1567(1.15); C1579(1.15)  LDD1264  [6]
 LDCM0386  CL116 PaTu 8988t C1567(0.98); C1579(0.98)  LDD1265  [6]
 LDCM0420  CL31 PaTu 8988t C1567(0.96); C1579(0.96)  LDD1299  [6]
 LDCM0421  CL32 PaTu 8988t C1567(0.68); C1579(0.68)  LDD1300  [6]
 LDCM0422  CL33 PaTu 8988t C1567(0.65); C1579(0.65)  LDD1301  [6]
 LDCM0423  CL34 PaTu 8988t C1567(0.68); C1579(0.68)  LDD1302  [6]
 LDCM0424  CL35 PaTu 8988t C1567(0.75); C1579(0.75)  LDD1303  [6]
 LDCM0425  CL36 PaTu 8988t C1567(0.53); C1579(0.53)  LDD1304  [6]
 LDCM0426  CL37 PaTu 8988t C1567(0.63); C1579(0.63)  LDD1305  [6]
 LDCM0428  CL39 PaTu 8988t C1567(0.96); C1579(0.96)  LDD1307  [6]
 LDCM0430  CL40 PaTu 8988t C1567(0.78); C1579(0.78)  LDD1309  [6]
 LDCM0431  CL41 PaTu 8988t C1567(0.50); C1579(0.50)  LDD1310  [6]
 LDCM0432  CL42 PaTu 8988t C1567(0.75); C1579(0.75)  LDD1311  [6]
 LDCM0433  CL43 PaTu 8988t C1567(0.44); C1579(0.44)  LDD1312  [6]
 LDCM0434  CL44 PaTu 8988t C1567(0.65); C1579(0.65)  LDD1313  [6]
 LDCM0435  CL45 PaTu 8988t C1567(0.66); C1579(0.66)  LDD1314  [6]
 LDCM0453  CL61 PaTu 8988t C1567(0.85); C1579(0.85)  LDD1332  [6]
 LDCM0454  CL62 PaTu 8988t C1567(1.21); C1579(1.21)  LDD1333  [6]
 LDCM0455  CL63 PaTu 8988t C1567(1.23); C1579(1.23)  LDD1334  [6]
 LDCM0456  CL64 PaTu 8988t C1567(1.38); C1579(1.38)  LDD1335  [6]
 LDCM0457  CL65 PaTu 8988t C1567(1.06); C1579(1.06)  LDD1336  [6]
 LDCM0458  CL66 PaTu 8988t C1567(2.07); C1579(2.07)  LDD1337  [6]
 LDCM0459  CL67 PaTu 8988t C1567(1.14); C1579(1.14)  LDD1338  [6]
 LDCM0460  CL68 PaTu 8988t C1567(1.13); C1579(1.13)  LDD1339  [6]
 LDCM0461  CL69 PaTu 8988t C1567(1.09); C1579(1.09)  LDD1340  [6]
 LDCM0463  CL70 PaTu 8988t C1567(1.07); C1579(1.07)  LDD1342  [6]
 LDCM0464  CL71 PaTu 8988t C1567(0.97); C1579(0.97)  LDD1343  [6]
 LDCM0465  CL72 PaTu 8988t C1567(0.94); C1579(0.94)  LDD1344  [6]
 LDCM0466  CL73 PaTu 8988t C1567(1.51); C1579(1.51)  LDD1345  [6]
 LDCM0467  CL74 PaTu 8988t C1567(0.90); C1579(0.90)  LDD1346  [6]
 LDCM0469  CL76 PaTu 8988t C1567(1.37); C1579(1.37)  LDD1348  [6]
 LDCM0470  CL77 PaTu 8988t C1567(0.95); C1579(0.95)  LDD1349  [6]
 LDCM0471  CL78 PaTu 8988t C1567(0.98); C1579(0.98)  LDD1350  [6]
 LDCM0472  CL79 PaTu 8988t C1567(1.47); C1579(1.47)  LDD1351  [6]
 LDCM0474  CL80 PaTu 8988t C1567(0.96); C1579(0.96)  LDD1353  [6]
 LDCM0475  CL81 PaTu 8988t C1567(0.67); C1579(0.67)  LDD1354  [6]
 LDCM0476  CL82 PaTu 8988t C1567(0.95); C1579(0.95)  LDD1355  [6]
 LDCM0477  CL83 PaTu 8988t C1567(1.01); C1579(1.01)  LDD1356  [6]
 LDCM0478  CL84 PaTu 8988t C1567(0.79); C1579(0.79)  LDD1357  [6]
 LDCM0479  CL85 PaTu 8988t C1567(1.14); C1579(1.14)  LDD1358  [6]
 LDCM0480  CL86 PaTu 8988t C1567(0.93); C1579(0.93)  LDD1359  [6]
 LDCM0481  CL87 PaTu 8988t C1567(0.98); C1579(0.98)  LDD1360  [6]
 LDCM0482  CL88 PaTu 8988t C1567(0.70); C1579(0.70)  LDD1361  [6]
 LDCM0483  CL89 PaTu 8988t C1567(0.80); C1579(0.80)  LDD1362  [6]
 LDCM0485  CL90 PaTu 8988t C1567(1.22); C1579(1.22)  LDD1364  [6]
 LDCM0486  CL91 PaTu 8988t C1567(0.25); C1579(0.25)  LDD1365  [6]
 LDCM0487  CL92 PaTu 8988t C1567(0.22); C1579(0.22)  LDD1366  [6]
 LDCM0488  CL93 PaTu 8988t C1567(0.27); C1579(0.27)  LDD1367  [6]
 LDCM0489  CL94 PaTu 8988t C1567(0.32); C1579(0.32)  LDD1368  [6]
 LDCM0490  CL95 PaTu 8988t C1567(0.26); C1579(0.26)  LDD1369  [6]
 LDCM0491  CL96 PaTu 8988t C1567(0.24); C1579(0.24)  LDD1370  [6]
 LDCM0492  CL97 PaTu 8988t C1567(0.28); C1579(0.28)  LDD1371  [6]
 LDCM0493  CL98 PaTu 8988t C1567(0.28); C1579(0.28)  LDD1372  [6]
 LDCM0494  CL99 PaTu 8988t C1567(0.25); C1579(0.25)  LDD1373  [6]
 LDCM0201  EV-3 T cell C517(4.66)  LDD0515  [7]
 LDCM0202  EV-93 T cell C1051(5.91)  LDD0514  [7]
 LDCM0468  Fragment33 PaTu 8988t C1567(1.19); C1579(1.19)  LDD1347  [6]
 LDCM0427  Fragment51 PaTu 8988t C1567(0.53); C1579(0.53)  LDD1306  [6]
 LDCM0022  KB02 T cell C1051(20.00); C517(20.00)  LDD1703  [7]
 LDCM0023  KB03 786-O C843(1.32)  LDD2664  [2]
 LDCM0024  KB05 G361 C351(1.95); C843(1.49); C1591(1.27)  LDD3311  [2]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Other
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Eukaryotic translation initiation factor 3 subunit E (EIF3E) EIF-3 subunit E family P60228

References

1 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
2 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
3 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
4 Multiplexed CuAAC Suzuki-Miyaura Labeling for Tandem Activity-Based Chemoproteomic Profiling. Anal Chem. 2021 Feb 2;93(4):2610-2618. doi: 10.1021/acs.analchem.0c04726. Epub 2021 Jan 20.
Mass spectrometry data entry: PXD022279
5 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
6 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
7 An Activity-Guided Map of Electrophile-Cysteine Interactions in Primary Human T Cells. Cell. 2020 Aug 20;182(4):1009-1026.e29. doi: 10.1016/j.cell.2020.07.001. Epub 2020 Jul 29.