Details of the Target

General Information of Target

Target ID LDTP07884
Target Name Ubiquitin carboxyl-terminal hydrolase 43 (USP43)
Gene Name USP43
Gene ID 124739
Synonyms
Ubiquitin carboxyl-terminal hydrolase 43; EC 3.4.19.12; Deubiquitinating enzyme 43; Ubiquitin thioesterase 43; Ubiquitin-specific-processing protease 43
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MDLGPGDAAGGGPLAPRPRRRRSLRRLFSRFLLALGSRSRPGDSPPRPQPGHCDGDGEGG
FACAPGPVPAAPGSPGEERPPGPQPQLQLPAGDGARPPGAQGLKNHGNTCFMNAVVQCLS
NTDLLAEFLALGRYRAAPGRAEVTEQLAALVRALWTREYTPQLSAEFKNAVSKYGSQFQG
NSQHDALEFLLWLLDRVHEDLEGSSRGPVSEKLPPEATKTSENCLSPSAQLPLGQSFVQS
HFQAQYRSSLTCPHCLKQSNTFDPFLCVSLPIPLRQTRFLSVTLVFPSKSQRFLRVGLAV
PILSTVAALRKMVAEEGGVPADEVILVELYPSGFQRSFFDEEDLNTIAEGDNVYAFQVPP
SPSQGTLSAHPLGLSASPRLAAREGQRFSLSLHSESKVLILFCNLVGSGQQASRFGPPFL
IREDRAVSWAQLQQSILSKVRHLMKSEAPVQNLGSLFSIRVVGLSVACSYLSPKDSRPLC
HWAVDRVLHLRRPGGPPHVKLAVEWDSSVKERLFGSLQEERAQDADSVWQQQQAHQQHSC
TLDECFQFYTKEEQLAQDDAWKCPHCQVLQQGMVKLSLWTLPDILIIHLKRFCQVGERRN
KLSTLVKFPLSGLNMAPHVAQRSTSPEAGLGPWPSWKQPDCLPTSYPLDFLYDLYAVCNH
HGNLQGGHYTAYCRNSLDGQWYSYDDSTVEPLREDEVNTRGAYILFYQKRNSIPPWSASS
SMRGSTSSSLSDHWLLRLGSHAGSTRGSLLSWSSAPCPSLPQVPDSPIFTNSLCNQEKGG
LEPRRLVRGVKGRSISMKAPTTSRAKQGPFKTMPLRWSFGSKEKPPGASVELVEYLESRR
RPRSTSQSIVSLLTGTAGEDEKSASPRSNVALPANSEDGGRAIERGPAGVPCPSAQPNHC
LAPGNSDGPNTARKLKENAGQDIKLPRKFDLPLTVMPSVEHEKPARPEGQKAMNWKESFQ
MGSKSSPPSPYMGFSGNSKDSRRGTSELDRPLQGTLTLLRSVFRKKENRRNERAEVSPQV
PPVSLVSGGLSPAMDGQAPGSPPALRIPEGLARGLGSRLERDVWSAPSSLRLPRKASRAP
RGSALGMSQRTVPGEQASYGTFQRVKYHTLSLGRKKTLPESSF
Target Bioclass
Enzyme
Family
Peptidase C19 family
Function May recognize and hydrolyze the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins.
Uniprot ID
Q70EL4
Ensemble ID
ENST00000285199.12
HGNC ID
HGNC:20072

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
ECC2 SNV: p.N771S DBIA    Probe Info 
HCT15 SNV: p.P564T .
HEC1 SNV: p.V606A .
ICC137 Deletion: p.T366LfsTer34 .
IGROV1 SNV: p.I584T .
JURKAT SNV: p.A170T; p.G920E .
LS180 SNV: p.G984D DBIA    Probe Info 
MDST8 SNV: p.P378S .
MELJUSO SNV: p.S821F .
MFE319 SNV: p.A381G .
MIAPACA2 SNV: p.P301L .
MOLT4 Deletion: p.V1023SfsTer4 .
PC14 SNV: p.L344R .
PC9 SNV: p.L344R .
PF382 SNV: p.R96W .
RL SNV: p.S740R .
SW756 SNV: p.P887A .
UACC62 SNV: p.H1108Y .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 2 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C892(0.84); C900(0.84)  LDD0078  [1]
NAIA_5
 Probe Info  		N.A.  LDD2224  [2]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0215  AC10 HCT 116 C892(0.89); C900(0.89)  LDD0532  [1]
 LDCM0216  AC100 HCT 116 C892(0.89); C900(0.89)  LDD0533  [1]
 LDCM0217  AC101 HCT 116 C892(0.93); C900(0.93)  LDD0534  [1]
 LDCM0218  AC102 HCT 116 C892(0.97); C900(0.97)  LDD0535  [1]
 LDCM0219  AC103 HCT 116 C892(1.14); C900(1.14)  LDD0536  [1]
 LDCM0220  AC104 HCT 116 C892(1.11); C900(1.11)  LDD0537  [1]
 LDCM0221  AC105 HCT 116 C892(1.15); C900(1.15)  LDD0538  [1]
 LDCM0222  AC106 HCT 116 C892(1.20); C900(1.20)  LDD0539  [1]
 LDCM0223  AC107 HCT 116 C892(1.21); C900(1.21)  LDD0540  [1]
 LDCM0224  AC108 HCT 116 C892(0.78); C900(0.78)  LDD0541  [1]
 LDCM0225  AC109 HCT 116 C892(0.84); C900(0.84)  LDD0542  [1]
 LDCM0226  AC11 HCT 116 C892(1.21); C900(1.21)  LDD0543  [1]
 LDCM0227  AC110 HCT 116 C892(0.88); C900(0.88)  LDD0544  [1]
 LDCM0228  AC111 HCT 116 C892(0.83); C900(0.83)  LDD0545  [1]
 LDCM0229  AC112 HCT 116 C892(1.01); C900(1.01)  LDD0546  [1]
 LDCM0237  AC12 HCT 116 C892(1.08); C900(1.08)  LDD0554  [1]
 LDCM0246  AC128 HCT 116 C892(0.88); C900(0.88)  LDD0563  [1]
 LDCM0247  AC129 HCT 116 C892(0.66); C900(0.66)  LDD0564  [1]
 LDCM0249  AC130 HCT 116 C892(0.77); C900(0.77)  LDD0566  [1]
 LDCM0250  AC131 HCT 116 C892(0.97); C900(0.97)  LDD0567  [1]
 LDCM0251  AC132 HCT 116 C892(0.67); C900(0.67)  LDD0568  [1]
 LDCM0252  AC133 HCT 116 C892(0.67); C900(0.67)  LDD0569  [1]
 LDCM0253  AC134 HCT 116 C892(0.75); C900(0.75)  LDD0570  [1]
 LDCM0254  AC135 HCT 116 C892(0.65); C900(0.65)  LDD0571  [1]
 LDCM0255  AC136 HCT 116 C892(0.67); C900(0.67)  LDD0572  [1]
 LDCM0256  AC137 HCT 116 C892(0.64); C900(0.64)  LDD0573  [1]
 LDCM0257  AC138 HCT 116 C892(0.64); C900(0.64)  LDD0574  [1]
 LDCM0258  AC139 HCT 116 C892(0.69); C900(0.69)  LDD0575  [1]
 LDCM0259  AC14 HCT 116 C892(1.05); C900(1.05)  LDD0576  [1]
 LDCM0260  AC140 HCT 116 C892(0.82); C900(0.82)  LDD0577  [1]
 LDCM0261  AC141 HCT 116 C892(0.79); C900(0.79)  LDD0578  [1]
 LDCM0262  AC142 HCT 116 C892(0.61); C900(0.61)  LDD0579  [1]
 LDCM0263  AC143 HCT 116 C892(1.20); C900(1.20)  LDD0580  [1]
 LDCM0264  AC144 HCT 116 C892(1.27); C900(1.27)  LDD0581  [1]
 LDCM0265  AC145 HCT 116 C892(1.06); C900(1.06)  LDD0582  [1]
 LDCM0266  AC146 HCT 116 C892(1.34); C900(1.34)  LDD0583  [1]
 LDCM0267  AC147 HCT 116 C892(1.27); C900(1.27)  LDD0584  [1]
 LDCM0268  AC148 HCT 116 C892(2.37); C900(2.37)  LDD0585  [1]
 LDCM0269  AC149 HCT 116 C892(1.62); C900(1.62)  LDD0586  [1]
 LDCM0270  AC15 HCT 116 C892(0.82); C900(0.82)  LDD0587  [1]
 LDCM0271  AC150 HCT 116 C892(1.07); C900(1.07)  LDD0588  [1]
 LDCM0272  AC151 HCT 116 C892(1.21); C900(1.21)  LDD0589  [1]
 LDCM0273  AC152 HCT 116 C892(1.59); C900(1.59)  LDD0590  [1]
 LDCM0274  AC153 HCT 116 C892(2.59); C900(2.59)  LDD0591  [1]
 LDCM0621  AC154 HCT 116 C892(1.58); C900(1.58)  LDD2158  [1]
 LDCM0622  AC155 HCT 116 C892(1.78); C900(1.78)  LDD2159  [1]
 LDCM0623  AC156 HCT 116 C892(1.19); C900(1.19)  LDD2160  [1]
 LDCM0624  AC157 HCT 116 C892(1.22); C900(1.22)  LDD2161  [1]
 LDCM0323  AC6 HCT 116 C892(1.31); C900(1.31)  LDD0640  [1]
 LDCM0332  AC68 HCT 116 C892(0.88); C900(0.88)  LDD0649  [1]
 LDCM0333  AC69 HCT 116 C892(1.25); C900(1.25)  LDD0650  [1]
 LDCM0334  AC7 HCT 116 C892(0.89); C900(0.89)  LDD0651  [1]
 LDCM0335  AC70 HCT 116 C892(0.85); C900(0.85)  LDD0652  [1]
 LDCM0336  AC71 HCT 116 C892(0.81); C900(0.81)  LDD0653  [1]
 LDCM0337  AC72 HCT 116 C892(1.47); C900(1.47)  LDD0654  [1]
 LDCM0338  AC73 HCT 116 C892(1.67); C900(1.67)  LDD0655  [1]
 LDCM0339  AC74 HCT 116 C892(1.29); C900(1.29)  LDD0656  [1]
 LDCM0340  AC75 HCT 116 C892(1.92); C900(1.92)  LDD0657  [1]
 LDCM0341  AC76 HCT 116 C892(1.08); C900(1.08)  LDD0658  [1]
 LDCM0342  AC77 HCT 116 C892(1.13); C900(1.13)  LDD0659  [1]
 LDCM0343  AC78 HCT 116 C892(0.97); C900(0.97)  LDD0660  [1]
 LDCM0344  AC79 HCT 116 C892(1.04); C900(1.04)  LDD0661  [1]
 LDCM0345  AC8 HCT 116 C892(1.15); C900(1.15)  LDD0662  [1]
 LDCM0346  AC80 HCT 116 C892(1.24); C900(1.24)  LDD0663  [1]
 LDCM0347  AC81 HCT 116 C892(1.30); C900(1.30)  LDD0664  [1]
 LDCM0348  AC82 HCT 116 C892(1.64); C900(1.64)  LDD0665  [1]
 LDCM0349  AC83 HCT 116 C892(1.95); C900(1.95)  LDD0666  [1]
 LDCM0350  AC84 HCT 116 C892(1.65); C900(1.65)  LDD0667  [1]
 LDCM0351  AC85 HCT 116 C892(1.21); C900(1.21)  LDD0668  [1]
 LDCM0352  AC86 HCT 116 C892(1.33); C900(1.33)  LDD0669  [1]
 LDCM0353  AC87 HCT 116 C892(1.30); C900(1.30)  LDD0670  [1]
 LDCM0354  AC88 HCT 116 C892(1.50); C900(1.50)  LDD0671  [1]
 LDCM0355  AC89 HCT 116 C892(1.57); C900(1.57)  LDD0672  [1]
 LDCM0357  AC90 HCT 116 C892(0.88); C900(0.88)  LDD0674  [1]
 LDCM0358  AC91 HCT 116 C892(2.30); C900(2.30)  LDD0675  [1]
 LDCM0359  AC92 HCT 116 C892(1.58); C900(1.58)  LDD0676  [1]
 LDCM0360  AC93 HCT 116 C892(1.34); C900(1.34)  LDD0677  [1]
 LDCM0361  AC94 HCT 116 C892(1.64); C900(1.64)  LDD0678  [1]
 LDCM0362  AC95 HCT 116 C892(1.52); C900(1.52)  LDD0679  [1]
 LDCM0363  AC96 HCT 116 C892(1.54); C900(1.54)  LDD0680  [1]
 LDCM0364  AC97 HCT 116 C892(1.70); C900(1.70)  LDD0681  [1]
 LDCM0365  AC98 HCT 116 C892(1.55); C900(1.55)  LDD0682  [1]
 LDCM0366  AC99 HCT 116 C892(0.90); C900(0.90)  LDD0683  [1]
 LDCM0248  AKOS034007472 HCT 116 C892(1.09); C900(1.09)  LDD0565  [1]
 LDCM0356  AKOS034007680 HCT 116 C892(1.24); C900(1.24)  LDD0673  [1]
 LDCM0275  AKOS034007705 HCT 116 C892(0.87); C900(0.87)  LDD0592  [1]
 LDCM0020  ARS-1620 HCC44 C892(0.84); C900(0.84)  LDD0078  [1]
 LDCM0632  CL-Sc Hep-G2 C468(0.79)  LDD2227  [2]
 LDCM0370  CL101 HCT 116 C892(1.18); C900(1.18)  LDD0687  [1]
 LDCM0371  CL102 HCT 116 C892(1.00); C900(1.00)  LDD0688  [1]
 LDCM0372  CL103 HCT 116 C892(1.09); C900(1.09)  LDD0689  [1]
 LDCM0373  CL104 HCT 116 C892(1.11); C900(1.11)  LDD0690  [1]
 LDCM0436  CL46 HCT 116 C892(1.13); C900(1.13)  LDD0753  [1]
 LDCM0437  CL47 HCT 116 C892(1.14); C900(1.14)  LDD0754  [1]
 LDCM0438  CL48 HCT 116 C892(0.79); C900(0.79)  LDD0755  [1]
 LDCM0439  CL49 HCT 116 C892(0.96); C900(0.96)  LDD0756  [1]
 LDCM0441  CL50 HCT 116 C892(0.99); C900(0.99)  LDD0758  [1]
 LDCM0442  CL51 HCT 116 C892(0.98); C900(0.98)  LDD0759  [1]
 LDCM0443  CL52 HCT 116 C892(1.23); C900(1.23)  LDD0760  [1]
 LDCM0444  CL53 HCT 116 C892(0.88); C900(0.88)  LDD0761  [1]
 LDCM0445  CL54 HCT 116 C892(1.23); C900(1.23)  LDD0762  [1]
 LDCM0446  CL55 HCT 116 C892(0.89); C900(0.89)  LDD0763  [1]
 LDCM0447  CL56 HCT 116 C892(0.87); C900(0.87)  LDD0764  [1]
 LDCM0448  CL57 HCT 116 C892(1.07); C900(1.07)  LDD0765  [1]
 LDCM0449  CL58 HCT 116 C892(1.03); C900(1.03)  LDD0766  [1]
 LDCM0450  CL59 HCT 116 C892(1.02); C900(1.02)  LDD0767  [1]
 LDCM0452  CL60 HCT 116 C892(0.99); C900(0.99)  LDD0769  [1]
 LDCM0453  CL61 HCT 116 C892(0.94); C900(0.94)  LDD0770  [1]
 LDCM0454  CL62 HCT 116 C892(1.31); C900(1.31)  LDD0771  [1]
 LDCM0455  CL63 HCT 116 C892(0.63); C900(0.63)  LDD0772  [1]
 LDCM0456  CL64 HCT 116 C892(0.53); C900(0.53)  LDD0773  [1]
 LDCM0457  CL65 HCT 116 C892(0.79); C900(0.79)  LDD0774  [1]
 LDCM0458  CL66 HCT 116 C892(0.58); C900(0.58)  LDD0775  [1]
 LDCM0459  CL67 HCT 116 C892(0.71); C900(0.71)  LDD0776  [1]
 LDCM0460  CL68 HCT 116 C892(0.71); C900(0.71)  LDD0777  [1]
 LDCM0461  CL69 HCT 116 C892(0.74); C900(0.74)  LDD0778  [1]
 LDCM0463  CL70 HCT 116 C892(0.69); C900(0.69)  LDD0780  [1]
 LDCM0464  CL71 HCT 116 C892(1.03); C900(1.03)  LDD0781  [1]
 LDCM0465  CL72 HCT 116 C892(0.72); C900(0.72)  LDD0782  [1]
 LDCM0466  CL73 HCT 116 C892(0.62); C900(0.62)  LDD0783  [1]
 LDCM0467  CL74 HCT 116 C892(0.76); C900(0.76)  LDD0784  [1]
 LDCM0468  Fragment33 HCT 116 C892(0.60); C900(0.60)  LDD0785  [1]
 LDCM0022  KB02 22RV1 C267(1.06)  LDD2243  [3]
 LDCM0023  KB03 22RV1 C267(2.16)  LDD2660  [3]
 LDCM0024  KB05 Hs 936.T C92(1.37)  LDD3313  [3]
 LDCM0021  THZ1 HCT 116 C892(1.18); C900(1.18); C563(1.07); C566(1.07)  LDD2173  [1]

References

1 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
2 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
3 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840