Details of the Target

General Information of Target

Target ID LDTP07346
Target Name Nuclear apoptosis-inducing factor 1 (NAIF1)
Gene Name NAIF1
Gene ID 203245
Synonyms
C9orf90; Nuclear apoptosis-inducing factor 1
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MAVPAKKRKMNFSEREVEIIVEELELKKHLLVNHFNAGVPLAAKSAAWHGILRRVNAVAT
CRRELPEVKKKWSDLKTEVRRKVAQVRAAVEGGEAPGPTEEDGAGGPGTGGGSGGGGPAV
APVLLTPMQQRICNLLGEATIISLPSTTEIHPVALGPSATAAAATVTLTQIPTETTYHTL
EEGVVEYCTAEAPPPLPPETPVDMMAQHADTSVKPQALKSRIALNSAKLIQEQRVTNLHV
KEIAQHLEQQNDLLQMIRRSQEVQACAQERQAQAMEGTQAALSVLIQVLRPMIKDFRRYL
QSNTANPAPASDPGQVAQNGQPDSIIQ
Target Bioclass
Other
Family
NAIF1 family
Subcellular location
Nucleus
Function Induces apoptosis.
Uniprot ID
Q69YI7
Ensemble ID
ENST00000373078.5
HGNC ID
HGNC:25446

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
A431 SNV: p.E14Q .
AN3CA SNV: p.R221C DBIA    Probe Info 
CAMA1 SNV: p.K44N; p.G103V; p.G156E .
HEC1 SNV: p.P322S DBIA    Probe Info 
HEC1B SNV: p.P322S .
HT115 SNV: p.N56S .
Ishikawa (Heraklio) 02 ER SNV: p.I20V; p.P201T DBIA    Probe Info 
JURKAT SNV: p.A95V .
LNCaP clone FGC SNV: p.S146I .
MCC26 SNV: p.E191Q .
MFE319 SNV: p.P107A DBIA    Probe Info 
MOLT4 SNV: p.D203N .
SNGM SNV: p.S143G DBIA    Probe Info 

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 4 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C266(14.95)  LDD0209  [1]
4-Iodoacetamidophenylacetylene
 Probe Info  		N.A.  LDD0038  [2]
IA-alkyne
 Probe Info  		N.A.  LDD0165  [3]
IPM
 Probe Info  		N.A.  LDD2156  [4]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0215  AC10 HEK-293T C266(1.09)  LDD1508  [5]
 LDCM0226  AC11 HEK-293T C266(0.93)  LDD1509  [5]
 LDCM0237  AC12 HEK-293T C266(0.92)  LDD1510  [5]
 LDCM0259  AC14 HEK-293T C266(0.92)  LDD1512  [5]
 LDCM0270  AC15 HEK-293T C266(1.40)  LDD1513  [5]
 LDCM0277  AC18 HEK-293T C266(0.87)  LDD1516  [5]
 LDCM0278  AC19 HEK-293T C266(0.88)  LDD1517  [5]
 LDCM0279  AC2 HEK-293T C266(0.84)  LDD1518  [5]
 LDCM0280  AC20 HEK-293T C266(0.74)  LDD1519  [5]
 LDCM0281  AC21 HEK-293T C266(1.08)  LDD1520  [5]
 LDCM0282  AC22 HEK-293T C266(0.75)  LDD1521  [5]
 LDCM0283  AC23 HEK-293T C266(1.09)  LDD1522  [5]
 LDCM0284  AC24 HEK-293T C266(0.93)  LDD1523  [5]
 LDCM0286  AC26 HEK-293T C266(0.94)  LDD1525  [5]
 LDCM0287  AC27 HEK-293T C266(1.16)  LDD1526  [5]
 LDCM0288  AC28 HEK-293T C266(0.94)  LDD1527  [5]
 LDCM0289  AC29 HEK-293T C266(1.08)  LDD1528  [5]
 LDCM0290  AC3 HEK-293T C266(0.87)  LDD1529  [5]
 LDCM0291  AC30 HEK-293T C266(0.90)  LDD1530  [5]
 LDCM0292  AC31 HEK-293T C266(1.12)  LDD1531  [5]
 LDCM0293  AC32 HEK-293T C266(1.22)  LDD1532  [5]
 LDCM0295  AC34 HEK-293T C266(0.95)  LDD1534  [5]
 LDCM0296  AC35 HCT 116 C61(1.02)  LDD0613  [6]
 LDCM0297  AC36 HCT 116 C61(1.09)  LDD0614  [6]
 LDCM0298  AC37 HCT 116 C61(1.11)  LDD0615  [6]
 LDCM0299  AC38 HCT 116 C61(1.02)  LDD0616  [6]
 LDCM0300  AC39 HCT 116 C61(1.02)  LDD0617  [6]
 LDCM0301  AC4 HEK-293T C266(0.95)  LDD1540  [5]
 LDCM0302  AC40 HCT 116 C61(1.13)  LDD0619  [6]
 LDCM0303  AC41 HCT 116 C61(1.14)  LDD0620  [6]
 LDCM0304  AC42 HCT 116 C61(1.08)  LDD0621  [6]
 LDCM0305  AC43 HCT 116 C61(1.06)  LDD0622  [6]
 LDCM0306  AC44 HCT 116 C61(1.14)  LDD0623  [6]
 LDCM0307  AC45 HCT 116 C61(1.10)  LDD0624  [6]
 LDCM0308  AC46 HCT 116 C61(1.10)  LDD0625  [6]
 LDCM0309  AC47 HCT 116 C61(1.08)  LDD0626  [6]
 LDCM0310  AC48 HCT 116 C61(1.38)  LDD0627  [6]
 LDCM0311  AC49 HCT 116 C61(1.29)  LDD0628  [6]
 LDCM0312  AC5 HEK-293T C266(0.91)  LDD1551  [5]
 LDCM0313  AC50 HCT 116 C61(1.28)  LDD0630  [6]
 LDCM0314  AC51 HCT 116 C61(1.10)  LDD0631  [6]
 LDCM0315  AC52 HCT 116 C61(1.11)  LDD0632  [6]
 LDCM0316  AC53 HCT 116 C61(1.14)  LDD0633  [6]
 LDCM0317  AC54 HCT 116 C61(1.22)  LDD0634  [6]
 LDCM0318  AC55 HCT 116 C61(1.21)  LDD0635  [6]
 LDCM0319  AC56 HCT 116 C61(1.47)  LDD0636  [6]
 LDCM0321  AC58 HEK-293T C266(1.06)  LDD1560  [5]
 LDCM0322  AC59 HEK-293T C266(0.95)  LDD1561  [5]
 LDCM0323  AC6 HEK-293T C266(0.90)  LDD1562  [5]
 LDCM0324  AC60 HEK-293T C266(0.94)  LDD1563  [5]
 LDCM0325  AC61 HEK-293T C266(0.97)  LDD1564  [5]
 LDCM0326  AC62 HEK-293T C266(0.97)  LDD1565  [5]
 LDCM0327  AC63 HEK-293T C266(1.30)  LDD1566  [5]
 LDCM0328  AC64 HEK-293T C266(0.98)  LDD1567  [5]
 LDCM0334  AC7 HEK-293T C266(1.21)  LDD1568  [5]
 LDCM0345  AC8 HEK-293T C266(0.95)  LDD1569  [5]
 LDCM0349  AC83 HCT 116 C61(1.07)  LDD0666  [6]
 LDCM0350  AC84 HCT 116 C61(0.91)  LDD0667  [6]
 LDCM0351  AC85 HCT 116 C61(0.93)  LDD0668  [6]
 LDCM0352  AC86 HCT 116 C61(0.81)  LDD0669  [6]
 LDCM0353  AC87 HCT 116 C61(1.06)  LDD0670  [6]
 LDCM0354  AC88 HCT 116 C61(0.88)  LDD0671  [6]
 LDCM0355  AC89 HCT 116 C61(1.39)  LDD0672  [6]
 LDCM0357  AC90 HCT 116 C61(0.75)  LDD0674  [6]
 LDCM0358  AC91 HCT 116 C61(0.99)  LDD0675  [6]
 LDCM0359  AC92 HCT 116 C61(1.08)  LDD0676  [6]
 LDCM0360  AC93 HCT 116 C61(0.85)  LDD0677  [6]
 LDCM0361  AC94 HCT 116 C61(1.00)  LDD0678  [6]
 LDCM0362  AC95 HCT 116 C61(1.03)  LDD0679  [6]
 LDCM0363  AC96 HCT 116 C61(1.07)  LDD0680  [6]
 LDCM0364  AC97 HCT 116 C61(0.92)  LDD0681  [6]
 LDCM0248  AKOS034007472 HEK-293T C266(1.05)  LDD1511  [5]
 LDCM0275  AKOS034007705 HEK-293T C266(0.82)  LDD1514  [5]
 LDCM0367  CL1 HEK-293T C266(0.74)  LDD1571  [5]
 LDCM0368  CL10 HEK-293T C266(1.21)  LDD1572  [5]
 LDCM0369  CL100 HEK-293T C266(0.94)  LDD1573  [5]
 LDCM0370  CL101 HEK-293T C266(1.07)  LDD1574  [5]
 LDCM0373  CL104 HEK-293T C266(0.80)  LDD1577  [5]
 LDCM0374  CL105 HEK-293T C266(1.08)  LDD1578  [5]
 LDCM0377  CL108 HEK-293T C266(0.90)  LDD1581  [5]
 LDCM0378  CL109 HEK-293T C266(0.98)  LDD1582  [5]
 LDCM0379  CL11 HEK-293T C266(1.40)  LDD1583  [5]
 LDCM0382  CL112 HEK-293T C266(1.07)  LDD1586  [5]
 LDCM0383  CL113 HEK-293T C266(0.86)  LDD1587  [5]
 LDCM0386  CL116 HEK-293T C266(0.75)  LDD1590  [5]
 LDCM0387  CL117 HCT 116 C61(1.40)  LDD0704  [6]
 LDCM0388  CL118 HCT 116 C61(0.97)  LDD0705  [6]
 LDCM0389  CL119 HCT 116 C61(1.07)  LDD0706  [6]
 LDCM0390  CL12 HEK-293T C266(0.82)  LDD1594  [5]
 LDCM0391  CL120 HCT 116 C61(1.16)  LDD0708  [6]
 LDCM0392  CL121 HCT 116 C61(1.06)  LDD0709  [6]
 LDCM0393  CL122 HCT 116 C61(0.94)  LDD0710  [6]
 LDCM0394  CL123 HCT 116 C61(1.03)  LDD0711  [6]
 LDCM0395  CL124 HCT 116 C61(1.14)  LDD0712  [6]
 LDCM0396  CL125 HEK-293T C266(0.90)  LDD1600  [5]
 LDCM0399  CL128 HEK-293T C266(0.85)  LDD1603  [5]
 LDCM0400  CL13 HEK-293T C266(1.03)  LDD1604  [5]
 LDCM0403  CL16 HEK-293T C266(0.88)  LDD1607  [5]
 LDCM0405  CL18 HEK-293T C266(0.82)  LDD1609  [5]
 LDCM0406  CL19 HEK-293T C266(1.14)  LDD1610  [5]
 LDCM0408  CL20 HEK-293T C266(1.49)  LDD1612  [5]
 LDCM0409  CL21 HEK-293T C266(1.13)  LDD1613  [5]
 LDCM0410  CL22 HEK-293T C266(1.18)  LDD1614  [5]
 LDCM0411  CL23 HEK-293T C266(1.60)  LDD1615  [5]
 LDCM0412  CL24 HEK-293T C266(0.97)  LDD1616  [5]
 LDCM0413  CL25 HEK-293T C266(0.76)  LDD1617  [5]
 LDCM0416  CL28 HEK-293T C266(0.86)  LDD1620  [5]
 LDCM0419  CL30 HEK-293T C266(0.87)  LDD1623  [5]
 LDCM0420  CL31 HEK-293T C266(0.97)  LDD1624  [5]
 LDCM0421  CL32 HEK-293T C266(1.02)  LDD1625  [5]
 LDCM0422  CL33 HEK-293T C266(1.19)  LDD1626  [5]
 LDCM0423  CL34 HEK-293T C266(1.22)  LDD1627  [5]
 LDCM0424  CL35 HEK-293T C266(1.76)  LDD1628  [5]
 LDCM0425  CL36 HEK-293T C266(1.04)  LDD1629  [5]
 LDCM0426  CL37 HEK-293T C266(0.86)  LDD1630  [5]
 LDCM0429  CL4 HEK-293T C266(0.83)  LDD1633  [5]
 LDCM0430  CL40 HEK-293T C266(0.85)  LDD1634  [5]
 LDCM0432  CL42 HEK-293T C266(1.14)  LDD1636  [5]
 LDCM0433  CL43 HEK-293T C266(1.01)  LDD1637  [5]
 LDCM0434  CL44 HEK-293T C266(1.19)  LDD1638  [5]
 LDCM0435  CL45 HEK-293T C266(0.96)  LDD1639  [5]
 LDCM0436  CL46 HCT 116 C61(0.93)  LDD0753  [6]
 LDCM0437  CL47 HCT 116 C61(0.75)  LDD0754  [6]
 LDCM0438  CL48 HCT 116 C61(1.01)  LDD0755  [6]
 LDCM0439  CL49 HCT 116 C61(0.84)  LDD0756  [6]
 LDCM0441  CL50 HCT 116 C61(0.87)  LDD0758  [6]
 LDCM0442  CL51 HCT 116 C61(0.96)  LDD0759  [6]
 LDCM0443  CL52 HCT 116 C61(1.15)  LDD0760  [6]
 LDCM0444  CL53 HCT 116 C61(0.81)  LDD0761  [6]
 LDCM0445  CL54 HCT 116 C61(0.79)  LDD0762  [6]
 LDCM0446  CL55 HCT 116 C61(0.62)  LDD0763  [6]
 LDCM0447  CL56 HCT 116 C61(0.92)  LDD0764  [6]
 LDCM0448  CL57 HCT 116 C61(1.02)  LDD0765  [6]
 LDCM0449  CL58 HCT 116 C61(1.56)  LDD0766  [6]
 LDCM0450  CL59 HCT 116 C61(0.99)  LDD0767  [6]
 LDCM0451  CL6 HEK-293T C266(1.04)  LDD1654  [5]
 LDCM0452  CL60 HCT 116 C61(1.22)  LDD0769  [6]
 LDCM0453  CL61 HEK-293T C266(0.87)  LDD1656  [5]
 LDCM0456  CL64 HEK-293T C266(0.94)  LDD1659  [5]
 LDCM0458  CL66 HEK-293T C266(0.87)  LDD1661  [5]
 LDCM0459  CL67 HEK-293T C266(0.99)  LDD1662  [5]
 LDCM0460  CL68 HEK-293T C266(1.14)  LDD1663  [5]
 LDCM0461  CL69 HEK-293T C266(1.07)  LDD1664  [5]
 LDCM0462  CL7 HEK-293T C266(0.97)  LDD1665  [5]
 LDCM0463  CL70 HEK-293T C266(1.07)  LDD1666  [5]
 LDCM0464  CL71 HEK-293T C266(1.57)  LDD1667  [5]
 LDCM0465  CL72 HEK-293T C266(1.04)  LDD1668  [5]
 LDCM0466  CL73 HEK-293T C266(0.86)  LDD1669  [5]
 LDCM0469  CL76 HEK-293T C266(0.69)  LDD1672  [5]
 LDCM0471  CL78 HEK-293T C266(0.96)  LDD1674  [5]
 LDCM0472  CL79 HEK-293T C266(0.99)  LDD1675  [5]
 LDCM0473  CL8 HEK-293T C266(1.52)  LDD1676  [5]
 LDCM0474  CL80 HEK-293T C266(1.13)  LDD1677  [5]
 LDCM0475  CL81 HEK-293T C266(1.44)  LDD1678  [5]
 LDCM0476  CL82 HEK-293T C266(0.89)  LDD1679  [5]
 LDCM0477  CL83 HEK-293T C266(1.20)  LDD1680  [5]
 LDCM0478  CL84 HEK-293T C266(0.92)  LDD1681  [5]
 LDCM0479  CL85 HEK-293T C266(0.83)  LDD1682  [5]
 LDCM0482  CL88 HEK-293T C266(0.93)  LDD1685  [5]
 LDCM0484  CL9 HEK-293T C266(1.17)  LDD1687  [5]
 LDCM0485  CL90 HEK-293T C266(0.85)  LDD1688  [5]
 LDCM0486  CL91 HEK-293T C266(0.92)  LDD1689  [5]
 LDCM0487  CL92 HEK-293T C266(1.24)  LDD1690  [5]
 LDCM0488  CL93 HEK-293T C266(0.96)  LDD1691  [5]
 LDCM0489  CL94 HEK-293T C266(1.22)  LDD1692  [5]
 LDCM0490  CL95 HEK-293T C266(1.61)  LDD1693  [5]
 LDCM0491  CL96 HEK-293T C266(0.87)  LDD1694  [5]
 LDCM0492  CL97 HEK-293T C266(0.86)  LDD1695  [5]
 LDCM0022  KB02 HEK-293T C266(0.78); C61(1.05)  LDD1492  [5]
 LDCM0023  KB03 Jurkat C266(14.95)  LDD0209  [1]
 LDCM0024  KB05 NCI-H2172 C266(1.95)  LDD3356  [7]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Transcription factor
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Transcription factor SOX-14 (SOX14) . O95416
Other
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Cyclin-D1-binding protein 1 (CCNDBP1) CCNDBP1 family O95273
Telomeric repeat-binding factor 2 (TERF2) . Q15554

References

1 Covalent Inhibition by a Natural Product-Inspired Latent Electrophile. J Am Chem Soc. 2023 May 24;145(20):11097-11109. doi: 10.1021/jacs.3c00598. Epub 2023 May 15.
2 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
3 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
4 Benchmarking Cleavable Biotin Tags for Peptide-Centric Chemoproteomics. J Proteome Res. 2022 May 6;21(5):1349-1358. doi: 10.1021/acs.jproteome.2c00174. Epub 2022 Apr 25.
Mass spectrometry data entry: PXD031019
5 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
6 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
7 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840