Details of the Target

General Information of Target

Target ID LDTP06870
Target Name Protein GREB1 (GREB1)
Gene Name GREB1
Gene ID 9687
Synonyms
KIAA0575; Protein GREB1; Gene regulated in breast cancer 1 protein
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MGNSYAGQLKTTRFEEVLHNSIEASLRSNNLVPRPIFSQLYLEAEQQLAALEGGSRVDNE
EEEEEGEGGLETNGPPNPFQLHPLPEGCCTTDGFCQAGKDLRLVSISNEPMDVPAGFLLV
GVKSPSLPDHLLVCAVDKRFLPDDNGHNALLGFSGNCVGCGKKGFCYFTEFSNHINLKLT
TQPKKQKHLKYYLVRNAQGTLTKGPLICWKGSEFRSRQIPASTCSSSLFPALESTAAFPS
EPVPGTNPSILMGAQQAGPASDHPSLNAAMGPAVFNGKDSPKCQQLAKNNLLALPRPSAL
GILSNSGPPKKRHKGWSPESPSAPDGGCPQGGGNRAKYESAGMSCVPQVGLVGPASVTFP
VVASGEPVSVPDNLLKICKAKPVIFKGHGNFPYLCGNLNDVVVSPLLYTCYQNSQSVSRA
YEQYGASAIQPISEEMQLLLTVYYLVQLAADQVPLMEDLEQIFLRSWRESHLTEIRQYQQ
APPQPFPPAPSAAAPVTSAQLPWLASLAASSCNDSVHVIECAYSLAEGLSEMFRLLVEGK
LAKTNYVVIICACRSAAIDSCIAVTGKYQARILSESLLTPAEYQKEVNYELVTGKVDSLG
AFFSTLCPEGDIDILLDKFHQENQGHISSSLAASSVTKAASLDVSGTPVCTSYNLEPHSI
RPFQLAVAQKLLSHVCSIADSSTQNLDLGSFEKVDFLICIPPSEVTYQQTLLHVWHSGVL
LELGLKKEHMTKQRVEQYVLKLDTEAQTKFKAFLQNSFQNPHTLFVLIHDHAHWDLVSST
VHNLYSQSDPSVGLVDRLLNCREVKEAPNIVTLHVTSFPYALQTQHTLISPYNEIHWPAS
CSNGVDLYHENKKYFGLSEFIESTLSGHSLPLLRYDSSFEAMVTALGKRFPRLHSAVIRT
FVLVQHYAAALMAVSGLPQMKNYTSVETLEITQNLLNSPKQCPCGHGLMVLLRVPCSPLA
VVAYERLAHVRARLALEEHFEIILGSPSSGVTVGKHFVKQLRMWQKIEDVEWRPQTYLEL
EGLPCILIFSGMDPHGESLPRSLRYCDLRLINSSCLVRTALEQELGLAAYFVSNEVPLEK
GARNEALESDAEKLSSTDNEDEELGTEGSTSEKRSPMKRERSRSHDSASSSLSSKASGSA
LGGESSAQPTALPQGEHARSPQPRGPAEEGRAPGEKQRPRASQGPPSAISRHSPGPTPQP
DCSLRTGQRSVQVSVTSSCSQLSSSSGSSSSSVAPAAGTWVLQASQCSLTKACRQPPIVF
LPKLVYDMVVSTDSSGLPKAASLLPSPSVMWASSFRPLLSKTMTSTEQSLYYRQWTVPRP
SHMDYGNRAEGRVDGFHPRRLLLSGPPQIGKTGAYLQFLSVLSRMLVRLTEVDVYDEEEI
NINLREESDWHYLQLSDPWPDLELFKKLPFDYIIHDPKYEDASLICSHYQGIKSEDRGMS
RKPEDLYVRRQTARMRLSKYAAYNTYHHCEQCHQYMGFHPRYQLYESTLHAFAFSYSMLG
EEIQLHFIIPKSKEHHFVFSQPGGQLESMRLPLVTDKSHEYIKSPTFTPTTGRHEHGLFN
LYHAMDGASHLHVLVVKEYEMAIYKKYWPNHIMLVLPSIFNSAGVGAAHFLIKELSYHNL
ELERNRQEELGIKPQDIWPFIVISDDSCVMWNVVDVNSAGERSREFSWSERNVSLKHIMQ
HIEAAPDIMHYALLGLRKWSSKTRASEVQEPFSRCHVHNFIILNVDLTQNVQYNQNRFLC
DDVDFNLRVHSAGLLLCRFNRFSVMKKQIVVGGHRSFHITSKVSDNSAAVVPAQYICAPD
SKHTFLAAPAQLLLEKFLQHHSHLFFPLSLKNHDHPVLSVDCYLNLGSQISVCYVSSRPH
SLNISCSDLLFSGLLLYLCDSFVGASFLKKFHFLKGATLCVICQDRSSLRQTVVRLELED
EWQFRLRDEFQTANAREDRPLFFLTGRHI
Target Bioclass
Other
Family
GREB1 family
Subcellular location
Membrane
Function May play a role in estrogen-stimulated cell proliferation. Acts as a regulator of hormone-dependent cancer growth in breast and prostate cancers.
Uniprot ID
Q4ZG55
Ensemble ID
ENST00000234142.9
HGNC ID
HGNC:24885

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
22RV1 SNV: p.S298P .
A172 SNV: p.L18F .
A2058 SNV: p.S1271F .
AN3CA SNV: p.P1235L .
CALU6 SNV: p.R1947Ter .
CCK81 Insertion: p.S1288LfsTer89
SNV: p.H471R		 .
CHL1 SNV: p.F1924V .
DOTC24510 SNV: p.F663L .
DU145 SNV: p.G98R;p.K1513Ter
Substitution: p.E722Ter		 .
HCC1419 SNV: p.L127P .
HT115 SNV: p.P392T .
HT1376 SNV: p.D876H .
IGROV1 SNV: p.Y738C DBIA    Probe Info 
JHH4 SNV: p.W1291Ter .
JURKAT SNV: p.D1033G .
MFE319 SNV: p.L459M .
NCIH1993 SNV: p.R1449L .
OAW28 SNV: p.E1614Q .
OSRC2 SNV: p.S1096C .
RKO SNV: p.K1301E .
SBC5 SNV: p.T12I .
SKMEL5 SNV: p.Q1212Ter .
SW1116 SNV: p.G1567S .
SW1990 SNV: p.R1939L .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 7 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
STPyne
 Probe Info  		K1176(9.03)  LDD0277  [1]
IPM
 Probe Info  		C1202(0.00); C1055(0.00)  LDD0241  [2]
Probe 1
 Probe Info  		Y738(20.21); Y1447(36.27)  LDD3495  [3]
DBIA
 Probe Info  		C200(1.84)  LDD3340  [4]
Curcusone 37
 Probe Info  		4.36  LDD0188  [5]
AHL-Pu-1
 Probe Info  		C1202(6.09)  LDD0171  [6]
NAIA_5
 Probe Info  		C1253(0.00); C166(0.00); C328(0.00); C208(0.00)  LDD2223  [7]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0026  4SU-RNA+native RNA DM93 C1202(6.09)  LDD0171  [6]
 LDCM0284  AC24 HEK-293T C208(0.94)  LDD1523  [8]
 LDCM0293  AC32 HEK-293T C208(0.89)  LDD1532  [8]
 LDCM0302  AC40 HEK-293T C208(0.93)  LDD1541  [8]
 LDCM0310  AC48 HEK-293T C208(1.04)  LDD1549  [8]
 LDCM0319  AC56 HEK-293T C208(1.05)  LDD1558  [8]
 LDCM0328  AC64 HEK-293T C208(1.01)  LDD1567  [8]
 LDCM0345  AC8 HEK-293T C208(1.02)  LDD1569  [8]
 LDCM0275  AKOS034007705 HEK-293T C208(1.04)  LDD1514  [8]
 LDCM0632  CL-Sc Hep-G2 C345(20.00); C1055(1.58); C561(1.27); C956(1.27)  LDD2227  [7]
 LDCM0367  CL1 HEK-293T C208(1.19)  LDD1571  [8]
 LDCM0369  CL100 HEK-293T C208(0.99)  LDD1573  [8]
 LDCM0370  CL101 HEK-293T C208(1.08)  LDD1574  [8]
 LDCM0373  CL104 HEK-293T C208(0.88)  LDD1577  [8]
 LDCM0374  CL105 HEK-293T C208(0.99)  LDD1578  [8]
 LDCM0377  CL108 HEK-293T C208(1.02)  LDD1581  [8]
 LDCM0378  CL109 HEK-293T C208(0.95)  LDD1582  [8]
 LDCM0382  CL112 HEK-293T C208(0.96)  LDD1586  [8]
 LDCM0383  CL113 HEK-293T C208(1.20)  LDD1587  [8]
 LDCM0386  CL116 HEK-293T C208(0.79)  LDD1590  [8]
 LDCM0387  CL117 HEK-293T C208(1.07)  LDD1591  [8]
 LDCM0390  CL12 HEK-293T C208(1.02)  LDD1594  [8]
 LDCM0391  CL120 HEK-293T C208(0.89)  LDD1595  [8]
 LDCM0392  CL121 HEK-293T C208(0.95)  LDD1596  [8]
 LDCM0395  CL124 HEK-293T C208(0.85)  LDD1599  [8]
 LDCM0396  CL125 HEK-293T C208(0.91)  LDD1600  [8]
 LDCM0399  CL128 HEK-293T C208(0.92)  LDD1603  [8]
 LDCM0400  CL13 HEK-293T C208(1.13)  LDD1604  [8]
 LDCM0403  CL16 HEK-293T C208(0.81)  LDD1607  [8]
 LDCM0412  CL24 HEK-293T C208(1.03)  LDD1616  [8]
 LDCM0413  CL25 HEK-293T C208(0.88)  LDD1617  [8]
 LDCM0416  CL28 HEK-293T C208(0.93)  LDD1620  [8]
 LDCM0425  CL36 HEK-293T C208(1.00)  LDD1629  [8]
 LDCM0426  CL37 HEK-293T C208(0.84)  LDD1630  [8]
 LDCM0429  CL4 HEK-293T C208(0.89)  LDD1633  [8]
 LDCM0430  CL40 HEK-293T C208(0.80)  LDD1634  [8]
 LDCM0438  CL48 HEK-293T C208(0.99)  LDD1642  [8]
 LDCM0439  CL49 HEK-293T C208(0.94)  LDD1643  [8]
 LDCM0443  CL52 HEK-293T C208(1.03)  LDD1646  [8]
 LDCM0452  CL60 HEK-293T C208(1.04)  LDD1655  [8]
 LDCM0453  CL61 HEK-293T C208(1.04)  LDD1656  [8]
 LDCM0456  CL64 HEK-293T C208(0.85)  LDD1659  [8]
 LDCM0465  CL72 HEK-293T C208(1.00)  LDD1668  [8]
 LDCM0466  CL73 HEK-293T C208(0.96)  LDD1669  [8]
 LDCM0469  CL76 HEK-293T C208(0.87)  LDD1672  [8]
 LDCM0478  CL84 HEK-293T C208(0.96)  LDD1681  [8]
 LDCM0479  CL85 HEK-293T C208(0.92)  LDD1682  [8]
 LDCM0482  CL88 HEK-293T C208(0.94)  LDD1685  [8]
 LDCM0491  CL96 HEK-293T C208(0.90)  LDD1694  [8]
 LDCM0492  CL97 HEK-293T C208(1.07)  LDD1695  [8]
 LDCM0033  Curcusone1d MCF-7 4.36  LDD0188  [5]
 LDCM0634  CY-0357 Hep-G2 C1202(1.16); C956(0.53)  LDD2228  [7]
 LDCM0022  KB02 A2058 C200(1.49)  LDD2253  [4]
 LDCM0023  KB03 A2058 C200(1.93)  LDD2670  [4]
 LDCM0024  KB05 NB1 C200(1.84)  LDD3340  [4]

References

1 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
2 Oxidant-Induced Bioconjugation for Protein Labeling in Live Cells. ACS Chem Biol. 2023 Jan 20;18(1):112-122. doi: 10.1021/acschembio.2c00740. Epub 2022 Dec 21.
3 An Azo Coupling-Based Chemoproteomic Approach to Systematically Profile the Tyrosine Reactivity in the Human Proteome. Anal Chem. 2021 Jul 27;93(29):10334-10342. doi: 10.1021/acs.analchem.1c01935. Epub 2021 Jul 12.
4 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
5 Total Synthesis and Target Identification of the Curcusone Diterpenes. J Am Chem Soc. 2021 Mar 24;143(11):4379-4386. doi: 10.1021/jacs.1c00557. Epub 2021 Mar 11.
6 Chemoproteomic capture of RNA binding activity in living cells. Nat Commun. 2023 Oct 7;14(1):6282. doi: 10.1038/s41467-023-41844-z.
Mass spectrometry data entry: PXD044625
7 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
8 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402