Details of the Target

General Information of Target

Target ID LDTP06056
Target Name Protein FAM53B (FAM53B)
Gene Name FAM53B
Gene ID 9679
Synonyms
KIAA0140; SMP; Protein FAM53B; Protein simplet
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MVMVLSESLSTRGADSIACGTFSRELHTPKKMSQGPTLFSCGIMENDRWRDLDRKCPLQI
DQPSTSIWECLPEKDSSLWHREAVTACAVTSLIKDLSISDHNGNPSAPPSKRQCRSLSFS
DEMSSCRTSWRPLGSKVWTPVEKRRCYSGGSVQRYSNGFSTMQRSSSFSLPSRANVLSSP
CDQAGLHHRFGGQPCQGVPGSAPCGQAGDTWSPDLHPVGGGRLDLQRSLSCSHEQFSFVE
YCPPSANSTPASTPELARRSSGLSRSRSQPCVLNDKKVGVKRRRPEEVQEQRPSLDLAKM
AQNCQTFSSLSCLSAGTEDCGPQSPFARHVSNTRAWTALLSASGPGGRTPAGTPVPEPLP
PSFDDHLACQEDLSCEESDSCALDEDCGRRAEPAAAWRDRGAPGNSLCSLDGELDIEQIE
KN
Target Bioclass
Other
Family
FAM53 family
Subcellular location
Nucleus
Function Acts as a regulator of Wnt signaling pathway by regulating beta-catenin (CTNNB1) nuclear localization.
Uniprot ID
Q14153
Ensemble ID
ENST00000280780.6
HGNC ID
HGNC:28968

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
COLO320 SNV: p.T65P .
COLO678 Substitution: p.F326P .
KYSE150 Deletion: p.F326RfsTer31 .
MOLT4 SNV: p.A301T .
RKO SNV: p.R112C; p.N175Y .
SW620 SNV: p.S268T .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 6 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
IA-alkyne
 Probe Info  		C87(5.59); C271(16.37)  LDD1704  [1]
DBIA
 Probe Info  		C195(1.11); C204(1.11)  LDD0078  [2]
NAIA_5
 Probe Info  		N.A.  LDD2225  [3]
Compound 10
 Probe Info  		N.A.  LDD2216  [4]
Compound 11
 Probe Info  		C146(0.00); C87(0.00)  LDD2213  [4]
IPM
 Probe Info  		C271(0.00); C87(0.00)  LDD2156  [5]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0214  AC1 HEK-293T C56(0.80)  LDD0812  [2]
 LDCM0215  AC10 HEK-293T C56(1.32)  LDD0813  [2]
 LDCM0216  AC100 HEK-293T C56(1.11)  LDD0814  [2]
 LDCM0217  AC101 HEK-293T C56(1.08)  LDD0815  [2]
 LDCM0218  AC102 HEK-293T C56(1.44)  LDD0816  [2]
 LDCM0219  AC103 HEK-293T C56(1.39)  LDD0817  [2]
 LDCM0220  AC104 HEK-293T C56(1.41)  LDD0818  [2]
 LDCM0221  AC105 HEK-293T C56(1.09)  LDD0819  [2]
 LDCM0222  AC106 HEK-293T C56(0.87)  LDD0820  [2]
 LDCM0223  AC107 HEK-293T C56(0.99)  LDD0821  [2]
 LDCM0224  AC108 HEK-293T C56(1.02)  LDD0822  [2]
 LDCM0225  AC109 HEK-293T C56(1.12)  LDD0823  [2]
 LDCM0226  AC11 HEK-293T C56(0.87)  LDD0824  [2]
 LDCM0227  AC110 HEK-293T C56(1.74)  LDD0825  [2]
 LDCM0228  AC111 HEK-293T C56(1.62)  LDD0826  [2]
 LDCM0229  AC112 HEK-293T C56(2.10)  LDD0827  [2]
 LDCM0230  AC113 HEK-293T C56(1.10)  LDD0828  [2]
 LDCM0231  AC114 HEK-293T C56(1.32)  LDD0829  [2]
 LDCM0232  AC115 HEK-293T C56(1.13)  LDD0830  [2]
 LDCM0233  AC116 HEK-293T C56(1.05)  LDD0831  [2]
 LDCM0234  AC117 HEK-293T C56(1.19)  LDD0832  [2]
 LDCM0235  AC118 HEK-293T C56(1.53)  LDD0833  [2]
 LDCM0236  AC119 HEK-293T C56(1.43)  LDD0834  [2]
 LDCM0237  AC12 HEK-293T C56(1.34)  LDD0835  [2]
 LDCM0238  AC120 HEK-293T C56(1.53)  LDD0836  [2]
 LDCM0239  AC121 HEK-293T C56(1.16)  LDD0837  [2]
 LDCM0240  AC122 HEK-293T C56(1.49)  LDD0838  [2]
 LDCM0241  AC123 HEK-293T C56(1.54)  LDD0839  [2]
 LDCM0242  AC124 HEK-293T C56(1.34)  LDD0840  [2]
 LDCM0243  AC125 HEK-293T C56(1.87)  LDD0841  [2]
 LDCM0244  AC126 HEK-293T C56(1.94)  LDD0842  [2]
 LDCM0245  AC127 HEK-293T C56(2.10)  LDD0843  [2]
 LDCM0246  AC128 HEK-293T C56(0.89)  LDD0844  [2]
 LDCM0247  AC129 HEK-293T C56(1.11)  LDD0845  [2]
 LDCM0249  AC130 HEK-293T C56(0.90)  LDD0847  [2]
 LDCM0250  AC131 HEK-293T C56(0.79)  LDD0848  [2]
 LDCM0251  AC132 HEK-293T C56(1.10)  LDD0849  [2]
 LDCM0252  AC133 HEK-293T C56(1.10)  LDD0850  [2]
 LDCM0253  AC134 HEK-293T C56(1.15)  LDD0851  [2]
 LDCM0254  AC135 HEK-293T C56(1.29)  LDD0852  [2]
 LDCM0255  AC136 HEK-293T C56(1.48)  LDD0853  [2]
 LDCM0256  AC137 HEK-293T C56(1.11)  LDD0854  [2]
 LDCM0257  AC138 HEK-293T C56(1.42)  LDD0855  [2]
 LDCM0258  AC139 HEK-293T C56(1.11)  LDD0856  [2]
 LDCM0259  AC14 HEK-293T C56(1.47)  LDD0857  [2]
 LDCM0260  AC140 HEK-293T C56(1.10)  LDD0858  [2]
 LDCM0261  AC141 HEK-293T C56(1.51)  LDD0859  [2]
 LDCM0262  AC142 HEK-293T C56(1.44)  LDD0860  [2]
 LDCM0270  AC15 HEK-293T C56(1.23)  LDD0868  [2]
 LDCM0276  AC17 HEK-293T C56(1.51)  LDD0874  [2]
 LDCM0277  AC18 HEK-293T C56(1.12)  LDD0875  [2]
 LDCM0278  AC19 HEK-293T C56(1.25)  LDD0876  [2]
 LDCM0279  AC2 HEK-293T C56(1.09)  LDD0877  [2]
 LDCM0280  AC20 HEK-293T C56(1.74)  LDD0878  [2]
 LDCM0281  AC21 HEK-293T C56(1.83)  LDD0879  [2]
 LDCM0282  AC22 HEK-293T C56(1.02)  LDD0880  [2]
 LDCM0283  AC23 HEK-293T C56(1.60)  LDD0881  [2]
 LDCM0284  AC24 HEK-293T C56(1.12)  LDD0882  [2]
 LDCM0285  AC25 HEK-293T C56(0.70)  LDD0883  [2]
 LDCM0286  AC26 HEK-293T C56(0.83)  LDD0884  [2]
 LDCM0287  AC27 HEK-293T C56(1.04)  LDD0885  [2]
 LDCM0288  AC28 HEK-293T C56(4.79)  LDD0886  [2]
 LDCM0289  AC29 HEK-293T C56(1.13)  LDD0887  [2]
 LDCM0290  AC3 HEK-293T C56(0.92)  LDD0888  [2]
 LDCM0291  AC30 HEK-293T C56(0.99)  LDD0889  [2]
 LDCM0292  AC31 HEK-293T C56(1.01)  LDD0890  [2]
 LDCM0293  AC32 HEK-293T C56(0.80)  LDD0891  [2]
 LDCM0294  AC33 HEK-293T C56(0.98)  LDD0892  [2]
 LDCM0295  AC34 HEK-293T C56(1.03)  LDD0893  [2]
 LDCM0296  AC35 HEK-293T C56(1.06)  LDD0894  [2]
 LDCM0297  AC36 HEK-293T C56(0.85)  LDD0895  [2]
 LDCM0298  AC37 HEK-293T C56(0.94)  LDD0896  [2]
 LDCM0299  AC38 HEK-293T C56(1.14)  LDD0897  [2]
 LDCM0300  AC39 HEK-293T C56(0.90)  LDD0898  [2]
 LDCM0301  AC4 HEK-293T C56(1.14)  LDD0899  [2]
 LDCM0302  AC40 HEK-293T C56(1.04)  LDD0900  [2]
 LDCM0303  AC41 HEK-293T C56(1.09)  LDD0901  [2]
 LDCM0304  AC42 HEK-293T C56(1.02)  LDD0902  [2]
 LDCM0305  AC43 HEK-293T C56(0.99)  LDD0903  [2]
 LDCM0306  AC44 HEK-293T C56(1.00)  LDD0904  [2]
 LDCM0307  AC45 HEK-293T C56(0.93)  LDD0905  [2]
 LDCM0308  AC46 HEK-293T C56(0.90)  LDD0906  [2]
 LDCM0309  AC47 HEK-293T C56(0.88)  LDD0907  [2]
 LDCM0310  AC48 HEK-293T C56(0.82)  LDD0908  [2]
 LDCM0311  AC49 HEK-293T C56(1.13)  LDD0909  [2]
 LDCM0312  AC5 HEK-293T C56(1.13)  LDD0910  [2]
 LDCM0313  AC50 HEK-293T C56(0.85)  LDD0911  [2]
 LDCM0314  AC51 HEK-293T C56(1.10)  LDD0912  [2]
 LDCM0315  AC52 HEK-293T C56(1.10)  LDD0913  [2]
 LDCM0316  AC53 HEK-293T C56(1.03)  LDD0914  [2]
 LDCM0317  AC54 HEK-293T C56(1.17)  LDD0915  [2]
 LDCM0318  AC55 HEK-293T C56(1.04)  LDD0916  [2]
 LDCM0319  AC56 HEK-293T C56(0.85)  LDD0917  [2]
 LDCM0322  AC59 HEK-293T C146(0.83)  LDD1561  [6]
 LDCM0323  AC6 HEK-293T C56(1.39)  LDD0921  [2]
 LDCM0324  AC60 HEK-293T C146(1.12)  LDD1563  [6]
 LDCM0332  AC68 HEK-293T C56(1.05)  LDD0930  [2]
 LDCM0333  AC69 HEK-293T C56(0.96)  LDD0931  [2]
 LDCM0334  AC7 HEK-293T C56(1.29)  LDD0932  [2]
 LDCM0335  AC70 HEK-293T C56(1.09)  LDD0933  [2]
 LDCM0336  AC71 HEK-293T C56(1.29)  LDD0934  [2]
 LDCM0337  AC72 HEK-293T C56(1.32)  LDD0935  [2]
 LDCM0338  AC73 HEK-293T C56(1.51)  LDD0936  [2]
 LDCM0339  AC74 HEK-293T C56(1.27)  LDD0937  [2]
 LDCM0340  AC75 HEK-293T C56(1.05)  LDD0938  [2]
 LDCM0341  AC76 HEK-293T C56(0.95)  LDD0939  [2]
 LDCM0342  AC77 HEK-293T C56(1.02)  LDD0940  [2]
 LDCM0343  AC78 HEK-293T C56(1.04)  LDD0941  [2]
 LDCM0344  AC79 HEK-293T C56(1.10)  LDD0942  [2]
 LDCM0345  AC8 HEK-293T C56(1.81)  LDD0943  [2]
 LDCM0346  AC80 HEK-293T C56(1.21)  LDD0944  [2]
 LDCM0347  AC81 HEK-293T C56(1.33)  LDD0945  [2]
 LDCM0348  AC82 HEK-293T C56(1.59)  LDD0946  [2]
 LDCM0349  AC83 HEK-293T C56(1.22)  LDD0947  [2]
 LDCM0350  AC84 HEK-293T C56(1.38)  LDD0948  [2]
 LDCM0351  AC85 HEK-293T C56(1.36)  LDD0949  [2]
 LDCM0352  AC86 HEK-293T C56(1.78)  LDD0950  [2]
 LDCM0353  AC87 HEK-293T C56(1.57)  LDD0951  [2]
 LDCM0354  AC88 HEK-293T C56(1.80)  LDD0952  [2]
 LDCM0355  AC89 HEK-293T C56(1.91)  LDD0953  [2]
 LDCM0357  AC90 HEK-293T C56(1.61)  LDD0955  [2]
 LDCM0358  AC91 HEK-293T C56(1.58)  LDD0956  [2]
 LDCM0359  AC92 HEK-293T C56(1.36)  LDD0957  [2]
 LDCM0360  AC93 HEK-293T C56(2.05)  LDD0958  [2]
 LDCM0361  AC94 HEK-293T C56(1.63)  LDD0959  [2]
 LDCM0362  AC95 HEK-293T C56(1.49)  LDD0960  [2]
 LDCM0363  AC96 HEK-293T C56(2.01)  LDD0961  [2]
 LDCM0364  AC97 HEK-293T C56(3.74)  LDD0962  [2]
 LDCM0365  AC98 HEK-293T C56(0.92)  LDD0963  [2]
 LDCM0366  AC99 HEK-293T C56(0.75)  LDD0964  [2]
 LDCM0248  AKOS034007472 HEK-293T C56(1.54)  LDD0846  [2]
 LDCM0356  AKOS034007680 HEK-293T C56(1.68)  LDD0954  [2]
 LDCM0275  AKOS034007705 HEK-293T C56(1.75)  LDD0873  [2]
 LDCM0020  ARS-1620 HCC44 C195(1.11); C204(1.11)  LDD0078  [2]
 LDCM0632  CL-Sc Hep-G2 C87(20.00)  LDD2227  [3]
 LDCM0369  CL100 HEK-293T C56(1.12)  LDD0967  [2]
 LDCM0370  CL101 HEK-293T C56(1.82)  LDD0968  [2]
 LDCM0371  CL102 HEK-293T C56(1.63)  LDD0969  [2]
 LDCM0372  CL103 HEK-293T C56(1.70)  LDD0970  [2]
 LDCM0373  CL104 HEK-293T C56(1.71)  LDD0971  [2]
 LDCM0374  CL105 HEK-293T C56(0.75)  LDD0972  [2]
 LDCM0375  CL106 HEK-293T C56(0.84)  LDD0973  [2]
 LDCM0376  CL107 HEK-293T C56(1.16)  LDD0974  [2]
 LDCM0377  CL108 HEK-293T C56(0.91)  LDD0975  [2]
 LDCM0378  CL109 HEK-293T C56(1.15)  LDD0976  [2]
 LDCM0380  CL110 HEK-293T C56(1.14)  LDD0978  [2]
 LDCM0381  CL111 HEK-293T C56(1.04)  LDD0979  [2]
 LDCM0382  CL112 HEK-293T C56(0.74)  LDD0980  [2]
 LDCM0383  CL113 HEK-293T C56(1.55)  LDD0981  [2]
 LDCM0384  CL114 HEK-293T C56(1.43)  LDD0982  [2]
 LDCM0385  CL115 HEK-293T C56(1.20)  LDD0983  [2]
 LDCM0386  CL116 HEK-293T C56(1.04)  LDD0984  [2]
 LDCM0387  CL117 HEK-293T C56(0.93)  LDD0985  [2]
 LDCM0388  CL118 HEK-293T C56(0.93)  LDD0986  [2]
 LDCM0389  CL119 HEK-293T C56(1.16)  LDD0987  [2]
 LDCM0391  CL120 HEK-293T C56(0.78)  LDD0989  [2]
 LDCM0392  CL121 HEK-293T C56(1.02)  LDD0990  [2]
 LDCM0393  CL122 HEK-293T C56(1.16)  LDD0991  [2]
 LDCM0394  CL123 HEK-293T C56(0.86)  LDD0992  [2]
 LDCM0395  CL124 HEK-293T C56(1.11)  LDD0993  [2]
 LDCM0406  CL19 HEK-293T C146(1.13)  LDD1610  [6]
 LDCM0408  CL20 HEK-293T C146(1.10)  LDD1612  [6]
 LDCM0420  CL31 HEK-293T C56(1.19)  LDD1018  [2]
 LDCM0421  CL32 HEK-293T C56(1.17)  LDD1019  [2]
 LDCM0422  CL33 HEK-293T C56(1.14)  LDD1020  [2]
 LDCM0423  CL34 HEK-293T C56(0.95)  LDD1021  [2]
 LDCM0424  CL35 HEK-293T C56(1.14)  LDD1022  [2]
 LDCM0425  CL36 HEK-293T C56(1.09)  LDD1023  [2]
 LDCM0426  CL37 HEK-293T C56(1.30)  LDD1024  [2]
 LDCM0428  CL39 HEK-293T C56(1.69)  LDD1026  [2]
 LDCM0430  CL40 HEK-293T C56(1.35)  LDD1028  [2]
 LDCM0431  CL41 HEK-293T C56(1.47)  LDD1029  [2]
 LDCM0432  CL42 HEK-293T C56(1.49)  LDD1030  [2]
 LDCM0433  CL43 HEK-293T C56(1.50)  LDD1031  [2]
 LDCM0434  CL44 HEK-293T C56(1.15)  LDD1032  [2]
 LDCM0435  CL45 HEK-293T C56(1.27)  LDD1033  [2]
 LDCM0446  CL55 HEK-293T C146(1.11)  LDD1649  [6]
 LDCM0447  CL56 HEK-293T C146(1.23)  LDD1650  [6]
 LDCM0453  CL61 HEK-293T C56(0.81)  LDD1051  [2]
 LDCM0454  CL62 HEK-293T C56(1.03)  LDD1052  [2]
 LDCM0455  CL63 HEK-293T C56(0.94)  LDD1053  [2]
 LDCM0456  CL64 HEK-293T C56(0.98)  LDD1054  [2]
 LDCM0457  CL65 HEK-293T C56(1.01)  LDD1055  [2]
 LDCM0458  CL66 HEK-293T C56(0.81)  LDD1056  [2]
 LDCM0459  CL67 HEK-293T C56(0.94)  LDD1057  [2]
 LDCM0460  CL68 HEK-293T C56(0.88)  LDD1058  [2]
 LDCM0461  CL69 HEK-293T C56(0.92)  LDD1059  [2]
 LDCM0462  CL7 HEK-293T C146(1.16)  LDD1665  [6]
 LDCM0463  CL70 HEK-293T C56(0.75)  LDD1061  [2]
 LDCM0464  CL71 HEK-293T C56(1.17)  LDD1062  [2]
 LDCM0465  CL72 HEK-293T C56(1.15)  LDD1063  [2]
 LDCM0466  CL73 HEK-293T C56(0.98)  LDD1064  [2]
 LDCM0467  CL74 HEK-293T C56(1.42)  LDD1065  [2]
 LDCM0469  CL76 HEK-293T C56(1.05)  LDD1067  [2]
 LDCM0470  CL77 HEK-293T C56(0.78)  LDD1068  [2]
 LDCM0471  CL78 HEK-293T C56(0.80)  LDD1069  [2]
 LDCM0472  CL79 HEK-293T C56(1.16)  LDD1070  [2]
 LDCM0473  CL8 HEK-293T C146(1.10)  LDD1676  [6]
 LDCM0474  CL80 HEK-293T C56(0.96)  LDD1072  [2]
 LDCM0475  CL81 HEK-293T C56(1.00)  LDD1073  [2]
 LDCM0476  CL82 HEK-293T C56(1.46)  LDD1074  [2]
 LDCM0477  CL83 HEK-293T C56(1.99)  LDD1075  [2]
 LDCM0478  CL84 HEK-293T C56(0.88)  LDD1076  [2]
 LDCM0479  CL85 HEK-293T C56(0.88)  LDD1077  [2]
 LDCM0480  CL86 HEK-293T C56(0.82)  LDD1078  [2]
 LDCM0481  CL87 HEK-293T C56(1.00)  LDD1079  [2]
 LDCM0482  CL88 HEK-293T C56(1.37)  LDD1080  [2]
 LDCM0483  CL89 HEK-293T C56(1.37)  LDD1081  [2]
 LDCM0485  CL90 HEK-293T C56(1.22)  LDD1083  [2]
 LDCM0486  CL91 HEK-293T C56(0.86)  LDD1084  [2]
 LDCM0487  CL92 HEK-293T C56(0.93)  LDD1085  [2]
 LDCM0488  CL93 HEK-293T C56(0.75)  LDD1086  [2]
 LDCM0489  CL94 HEK-293T C56(1.01)  LDD1087  [2]
 LDCM0490  CL95 HEK-293T C56(1.46)  LDD1088  [2]
 LDCM0491  CL96 HEK-293T C56(1.08)  LDD1089  [2]
 LDCM0492  CL97 HEK-293T C56(1.06)  LDD1090  [2]
 LDCM0493  CL98 HEK-293T C56(0.89)  LDD1091  [2]
 LDCM0494  CL99 HEK-293T C56(0.89)  LDD1092  [2]
 LDCM0468  Fragment33 HEK-293T C56(1.28)  LDD1066  [2]
 LDCM0427  Fragment51 HEK-293T C56(1.11)  LDD1025  [2]
 LDCM0022  KB02 T cell-activated C87(5.59); C271(16.37)  LDD1704  [1]
 LDCM0023  KB03 KMS11 C271(5.29)  LDD2839  [7]
 LDCM0024  KB05 RPMI-8226 C271(2.39)  LDD3411  [7]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Other
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Dynein light chain 1, cytoplasmic (DYNLL1) Dynein light chain family P63167

References

1 An Activity-Guided Map of Electrophile-Cysteine Interactions in Primary Human T Cells. Cell. 2020 Aug 20;182(4):1009-1026.e29. doi: 10.1016/j.cell.2020.07.001. Epub 2020 Jul 29.
2 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
3 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
4 Multiplexed CuAAC Suzuki-Miyaura Labeling for Tandem Activity-Based Chemoproteomic Profiling. Anal Chem. 2021 Feb 2;93(4):2610-2618. doi: 10.1021/acs.analchem.0c04726. Epub 2021 Jan 20.
Mass spectrometry data entry: PXD022279
5 Benchmarking Cleavable Biotin Tags for Peptide-Centric Chemoproteomics. J Proteome Res. 2022 May 6;21(5):1349-1358. doi: 10.1021/acs.jproteome.2c00174. Epub 2022 Apr 25.
Mass spectrometry data entry: PXD031019
6 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
7 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840