Details of the Target

General Information of Target

Target ID LDTP05048
Target Name Small ubiquitin-related modifier 1 (SUMO1)
Gene Name SUMO1
Gene ID 7341
Synonyms
SMT3C; SMT3H3; UBL1; Small ubiquitin-related modifier 1; SUMO-1; GAP-modifying protein 1; GMP1; SMT3 homolog 3; Sentrin; Ubiquitin-homology domain protein PIC1; Ubiquitin-like protein SMT3C; Smt3C; Ubiquitin-like protein UBL1
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTHLKKLKESYCQRQGVPMN
SLRFLFEGQRIADNHTPKELGMEEEDVIEVYQEQTGGHSTV
Target Bioclass
Other
Family
Ubiquitin family, SUMO subfamily
Subcellular location
Nucleus membrane
Function
Ubiquitin-like protein that can be covalently attached to proteins as a monomer or a lysine-linked polymer. Covalent attachment via an isopeptide bond to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by E3 ligases such as PIAS1-4, RANBP2 or CBX4. This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Involved for instance in targeting RANGAP1 to the nuclear pore complex protein RANBP2. Covalently attached to the voltage-gated potassium channel KCNB1; this modulates the gating characteristics of KCNB1. Polymeric SUMO1 chains are also susceptible to polyubiquitination which functions as a signal for proteasomal degradation of modified proteins. May also regulate a network of genes involved in palate development. Covalently attached to ZFHX3.
Uniprot ID
P63165
Ensemble ID
ENST00000392244.7
HGNC ID
HGNC:12502
ChEMBL ID
CHEMBL2146296

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
IGROV1 Deletion: p.G97VfsTer22 .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 18 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
STPyne
 Probe Info  		K25(1.25); K37(9.55); K45(9.09)  LDD0277  [1]
ONAyne
 Probe Info  		K37(10.00)  LDD0275  [1]
DBIA
 Probe Info  		C52(1.00)  LDD3330  [2]
BTD
 Probe Info  		C52(1.70)  LDD1700  [3]
AMP probe
 Probe Info  		K37(0.00); K39(0.00)  LDD0200  [4]
ATP probe
 Probe Info  		K37(0.00); K39(0.00); K25(0.00); K23(0.00)  LDD0199  [4]
4-Iodoacetamidophenylacetylene
 Probe Info  		N.A.  LDD0038  [5]
IA-alkyne
 Probe Info  		N.A.  LDD0036  [5]
Lodoacetamide azide
 Probe Info  		N.A.  LDD0037  [5]
IPM
 Probe Info  		N.A.  LDD0147  [6]
NHS
 Probe Info  		K7(0.00); K16(0.00); K17(0.00); K37(0.00)  LDD0010  [7]
SF
 Probe Info  		Y21(0.00); K23(0.00); K37(0.00)  LDD0028  [8]
TFBX
 Probe Info  		N.A.  LDD0148  [6]
1c-yne
 Probe Info  		N.A.  LDD0228  [9]
Acrolein
 Probe Info  		N.A.  LDD0217  [10]
Crotonaldehyde
 Probe Info  		N.A.  LDD0219  [10]
Methacrolein
 Probe Info  		N.A.  LDD0218  [10]
NAIA_5
 Probe Info  		N.A.  LDD2223  [11]
PAL-AfBPP Probe
Click To Hide/Show 1 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
VE-P
 Probe Info  		N.A.  LDD0396  [12]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0548  1-(4-(Benzo[d][1,3]dioxol-5-ylmethyl)piperazin-1-yl)-2-nitroethan-1-one MDA-MB-231 C52(0.37)  LDD2142  [3]
 LDCM0519  1-(6-methoxy-3,4-dihydroquinolin-1(2H)-yl)-2-nitroethan-1-one MDA-MB-231 C52(0.64)  LDD2112  [3]
 LDCM0502  1-(Cyanoacetyl)piperidine MDA-MB-231 C52(0.72)  LDD2095  [3]
 LDCM0537  2-Cyano-N,N-dimethylacetamide MDA-MB-231 C52(1.46)  LDD2130  [3]
 LDCM0524  2-Cyano-N-(2-morpholin-4-yl-ethyl)-acetamide MDA-MB-231 C52(1.00)  LDD2117  [3]
 LDCM0510  3-(4-(Hydroxydiphenylmethyl)piperidin-1-yl)-3-oxopropanenitrile MDA-MB-231 C52(1.09)  LDD2103  [3]
 LDCM0520  AKOS000195272 MDA-MB-231 C52(1.05)  LDD2113  [3]
 LDCM0498  BS-3668 MDA-MB-231 C52(0.78)  LDD2091  [3]
 LDCM0108  Chloroacetamide HeLa N.A.  LDD0222  [10]
 LDCM0625  F8 Ramos C52(0.99)  LDD2187  [13]
 LDCM0572  Fragment10 Ramos C52(0.60)  LDD2189  [13]
 LDCM0573  Fragment11 Ramos C52(0.17)  LDD2190  [13]
 LDCM0574  Fragment12 Ramos C52(0.64)  LDD2191  [13]
 LDCM0575  Fragment13 Ramos C52(1.15)  LDD2192  [13]
 LDCM0576  Fragment14 Ramos C52(0.82)  LDD2193  [13]
 LDCM0579  Fragment20 Ramos C52(0.56)  LDD2194  [13]
 LDCM0580  Fragment21 Ramos C52(0.94)  LDD2195  [13]
 LDCM0582  Fragment23 Ramos C52(1.61)  LDD2196  [13]
 LDCM0578  Fragment27 Ramos C52(1.32)  LDD2197  [13]
 LDCM0586  Fragment28 Ramos C52(0.93)  LDD2198  [13]
 LDCM0588  Fragment30 Ramos C52(1.39)  LDD2199  [13]
 LDCM0589  Fragment31 Ramos C52(1.21)  LDD2200  [13]
 LDCM0590  Fragment32 Ramos C52(0.83)  LDD2201  [13]
 LDCM0468  Fragment33 Ramos C52(2.51)  LDD2202  [13]
 LDCM0596  Fragment38 Ramos C52(1.54)  LDD2203  [13]
 LDCM0566  Fragment4 Ramos C52(0.69)  LDD2184  [13]
 LDCM0610  Fragment52 Ramos C52(1.28)  LDD2204  [13]
 LDCM0614  Fragment56 Ramos C52(2.06)  LDD2205  [13]
 LDCM0569  Fragment7 Ramos C52(0.49)  LDD2186  [13]
 LDCM0571  Fragment9 Ramos C52(0.46)  LDD2188  [13]
 LDCM0107  IAA HeLa N.A.  LDD0221  [10]
 LDCM0022  KB02 Ramos C52(0.51)  LDD2182  [13]
 LDCM0023  KB03 Ramos C52(0.68)  LDD2183  [13]
 LDCM0024  KB05 MIA PaCa-2 C52(1.00)  LDD3330  [2]
 LDCM0509  N-(4-bromo-3,5-dimethylphenyl)-2-nitroacetamide MDA-MB-231 C52(0.76)  LDD2102  [3]
 LDCM0528  N-(4-bromophenyl)-2-cyano-N-phenylacetamide MDA-MB-231 C52(0.99)  LDD2121  [3]
 LDCM0496  Nucleophilic fragment 11a MDA-MB-231 C52(0.44)  LDD2089  [3]
 LDCM0497  Nucleophilic fragment 11b MDA-MB-231 C52(1.27)  LDD2090  [3]
 LDCM0499  Nucleophilic fragment 12b MDA-MB-231 C52(0.90)  LDD2092  [3]
 LDCM0500  Nucleophilic fragment 13a MDA-MB-231 C52(1.33)  LDD2093  [3]
 LDCM0501  Nucleophilic fragment 13b MDA-MB-231 C52(1.20)  LDD2094  [3]
 LDCM0503  Nucleophilic fragment 14b MDA-MB-231 C52(0.45)  LDD2096  [3]
 LDCM0504  Nucleophilic fragment 15a MDA-MB-231 C52(0.80)  LDD2097  [3]
 LDCM0505  Nucleophilic fragment 15b MDA-MB-231 C52(0.74)  LDD2098  [3]
 LDCM0506  Nucleophilic fragment 16a MDA-MB-231 C52(0.97)  LDD2099  [3]
 LDCM0507  Nucleophilic fragment 16b MDA-MB-231 C52(0.47)  LDD2100  [3]
 LDCM0508  Nucleophilic fragment 17a MDA-MB-231 C52(1.55)  LDD2101  [3]
 LDCM0513  Nucleophilic fragment 19b MDA-MB-231 C52(0.43)  LDD2106  [3]
 LDCM0514  Nucleophilic fragment 20a MDA-MB-231 C52(0.98)  LDD2107  [3]
 LDCM0515  Nucleophilic fragment 20b MDA-MB-231 C52(0.62)  LDD2108  [3]
 LDCM0516  Nucleophilic fragment 21a MDA-MB-231 C52(0.65)  LDD2109  [3]
 LDCM0517  Nucleophilic fragment 21b MDA-MB-231 C52(0.45)  LDD2110  [3]
 LDCM0518  Nucleophilic fragment 22a MDA-MB-231 C52(1.00)  LDD2111  [3]
 LDCM0521  Nucleophilic fragment 23b MDA-MB-231 C52(0.66)  LDD2114  [3]
 LDCM0522  Nucleophilic fragment 24a MDA-MB-231 C52(0.50)  LDD2115  [3]
 LDCM0523  Nucleophilic fragment 24b MDA-MB-231 C52(0.73)  LDD2116  [3]
 LDCM0525  Nucleophilic fragment 25b MDA-MB-231 C52(0.52)  LDD2118  [3]
 LDCM0526  Nucleophilic fragment 26a MDA-MB-231 C52(2.98)  LDD2119  [3]
 LDCM0527  Nucleophilic fragment 26b MDA-MB-231 C52(0.99)  LDD2120  [3]
 LDCM0529  Nucleophilic fragment 27b MDA-MB-231 C52(0.32)  LDD2122  [3]
 LDCM0530  Nucleophilic fragment 28a MDA-MB-231 C52(0.74)  LDD2123  [3]
 LDCM0531  Nucleophilic fragment 28b MDA-MB-231 C52(0.35)  LDD2124  [3]
 LDCM0532  Nucleophilic fragment 29a MDA-MB-231 C52(0.78)  LDD2125  [3]
 LDCM0533  Nucleophilic fragment 29b MDA-MB-231 C52(0.30)  LDD2126  [3]
 LDCM0534  Nucleophilic fragment 30a MDA-MB-231 C52(0.92)  LDD2127  [3]
 LDCM0535  Nucleophilic fragment 30b MDA-MB-231 C52(0.73)  LDD2128  [3]
 LDCM0536  Nucleophilic fragment 31 MDA-MB-231 C52(1.18)  LDD2129  [3]
 LDCM0541  Nucleophilic fragment 36 MDA-MB-231 C52(0.34)  LDD2134  [3]
 LDCM0542  Nucleophilic fragment 37 MDA-MB-231 C52(1.32)  LDD2135  [3]
 LDCM0543  Nucleophilic fragment 38 MDA-MB-231 C52(1.01)  LDD2136  [3]
 LDCM0544  Nucleophilic fragment 39 MDA-MB-231 C52(0.79)  LDD2137  [3]
 LDCM0211  Nucleophilic fragment 3b MDA-MB-231 C52(1.70)  LDD1700  [3]
 LDCM0546  Nucleophilic fragment 40 MDA-MB-231 C52(0.74)  LDD2140  [3]
 LDCM0547  Nucleophilic fragment 41 MDA-MB-231 C52(0.44)  LDD2141  [3]
 LDCM0549  Nucleophilic fragment 43 MDA-MB-231 C52(0.76)  LDD2143  [3]
 LDCM0550  Nucleophilic fragment 5a MDA-MB-231 C52(3.77)  LDD2144  [3]
 LDCM0551  Nucleophilic fragment 5b MDA-MB-231 C52(1.00)  LDD2145  [3]
 LDCM0552  Nucleophilic fragment 6a MDA-MB-231 C52(0.86)  LDD2146  [3]
 LDCM0553  Nucleophilic fragment 6b MDA-MB-231 C52(1.53)  LDD2147  [3]
 LDCM0554  Nucleophilic fragment 7a MDA-MB-231 C52(0.63)  LDD2148  [3]
 LDCM0555  Nucleophilic fragment 7b MDA-MB-231 C52(0.74)  LDD2149  [3]
 LDCM0556  Nucleophilic fragment 8a MDA-MB-231 C52(0.63)  LDD2150  [3]
 LDCM0559  Nucleophilic fragment 9b MDA-MB-231 C52(2.91)  LDD2153  [3]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 20 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Poly [ADP-ribose] polymerase 1 (PARP1) ARTD/PARP family P09874
DNA (cytosine-5)-methyltransferase 3B (DNMT3B) C5-methyltransferase family Q9UBC3
Probable E3 ubiquitin-protein ligase DTX2 (DTX2) Deltex family Q86UW9
SUMO-specific isopeptidase USPL1 (USPL1) Peptidase C19 family Q5W0Q7
Sentrin-specific protease 1 (SENP1) Peptidase C48 family Q9P0U3
Sentrin-specific protease 2 (SENP2) Peptidase C48 family Q9HC62
E3 SUMO-protein ligase PIAS2 (PIAS2) PIAS family O75928
Helicase ARIP4 (RAD54L2) SNF2/RAD54 helicase family Q9Y4B4
TNF receptor-associated factor 2 (TRAF2) TNF receptor-associated factor family Q12933
TNF receptor-associated factor 4 (TRAF4) TNF receptor-associated factor family Q9BUZ4
SUMO-activating enzyme subunit 2 (UBA2) Ubiquitin-activating E1 family Q9UBT2
SUMO-conjugating enzyme UBC9 (UBE2I) Ubiquitin-conjugating enzyme family P63279
G/T mismatch-specific thymine DNA glycosylase (TDG) TDG/mug family Q13569
Breast cancer type 1 susceptibility protein (BRCA1) . P38398
BTB/POZ domain-containing protein 3 (BTBD3) . Q9Y2F9
E3 SUMO-protein ligase CBX4 (CBX4) . O00257
E3 ubiquitin-protein ligase RNF4 (RNF4) . P78317
Protein ARK2N (ARK2N) . Q96B23
Protein PML (PML) . P29590
RWD domain-containing protein 3 (RWDD3) . Q9Y3V2
Transporter and channel
Click To Hide/Show 4 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Huntingtin (HTT) Huntingtin family P42858
Cellular tumor antigen p53 (TP53) P53 family P04637
Ran GTPase-activating protein 1 (RANGAP1) RNA1 family P46060
Potassium channel subfamily K member 1 (KCNK1) Two pore domain potassium channel (TC 1.A.1.8) family O00180
Transcription factor
Click To Hide/Show 21 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
DNA-binding protein SATB1 (SATB1) CUT homeobox family Q01826
ETS domain-containing protein Elk-1 (ELK1) ETS family P19419
Alpha-globin transcription factor CP2 (TFCP2) Grh/CP2 family Q12800
Heat shock factor protein 1 (HSF1) HSF family Q00613
Interferon regulatory factor 1 (IRF1) IRF family P10914
Zinc finger protein 42 homolog (ZFP42) Krueppel C2H2-type zinc-finger protein family Q96MM3
Retinoic acid receptor alpha (RARA) Nuclear hormone receptor family P10276
Androgen receptor (AR) Nuclear hormone receptor family P10275
Homeobox protein prophet of Pit-1 (PROP1) Paired homeobox family O75360
Rhox homeobox family member 2 (RHOXF2) Paired-like homeobox family Q9BQY4
Hypoxia-inducible factor 1-alpha (HIF1A) . Q16665
Methyl-CpG-binding domain protein 1 (MBD1) . Q9UIS9
Nuclear autoantigen Sp-100 (SP100) . P23497
Nuclear factor NF-kappa-B p100 subunit (NFKB2) . Q00653
Transcription factor SOX-10 (SOX10) . P56693
Transcription factor SOX-2 (SOX2) . P48431
Transcriptional activator Myb (MYB) . P10242
Zinc finger and BTB domain-containing protein 2 (ZBTB2) . Q8N680
Zinc finger and BTB domain-containing protein 26 (ZBTB26) . Q9HCK0
Zinc finger and BTB domain-containing protein 6 (ZBTB6) . Q15916
Zinc finger MYM-type protein 5 (ZMYM5) . Q9UJ78
Cytokine and receptor
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Cyclic AMP-dependent transcription factor ATF-2 (ATF2) BZIP family P15336
Other
Click To Hide/Show 19 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Deuterosome assembly protein 1 (DEUP1) CEP63 family Q05D60
Death domain-associated protein 6 (DAXX) DAXX family Q9UER7
Eukaryotic translation initiation factor 4E (EIF4E) Eukaryotic initiation factor 4E family P06730
Protein FAM118B (FAM118B) FAM118 family Q9BPY3
PC4 and SFRS1-interacting protein (PSIP1) HDGF family O75475
MAPK-interacting and spindle-stabilizing protein-like (MAPK1IP1L) MISS family Q8NDC0
Heterogeneous nuclear ribonucleoproteins C1/C2 (HNRNPC) RRM HNRPC family P07910
U4/U6.U5 tri-snRNP-associated protein 1 (SART1) SNU66/SART1 family O43290
TNF receptor-associated factor 5 (TRAF5) TNF receptor-associated factor family O00463
Small ubiquitin-related modifier 5 (SUMO1P1) Ubiquitin family G2XKQ0
Zinc finger CCHC domain-containing protein 12 (ZCCHC12) ZCCHC12 family Q6PEW1
Axin-1 (AXIN1) . O15169
Caspase recruitment domain-containing protein 9 (CARD9) . Q9H257
Ectodysplasin-A receptor-associated adapter protein (EDARADD) . Q8WWZ3
Fibrinogen silencer-binding protein (FSBP) . O95073
Hepatocyte growth factor-regulated tyrosine kinase substrate (HGS) . O14964
NF-kappa-B essential modulator (IKBKG) . Q9Y6K9
TRAF family member-associated NF-kappa-B activator (TANK) . Q92844
Zinc finger CCHC domain-containing protein 7 (ZCCHC7) . Q8N3Z6

References

1 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
2 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
3 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
4 Targeted Proteomic Approaches for Proteome-Wide Characterizations of the AMP-Binding Capacities of Kinases. J Proteome Res. 2022 Aug 5;21(8):2063-2070. doi: 10.1021/acs.jproteome.2c00225. Epub 2022 Jul 12.
5 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
6 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
7 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
8 Solid Phase Synthesis of Fluorosulfate Containing Macrocycles for Chemoproteomic Workflows. bioRxiv [Preprint]. 2023 Feb 18:2023.02.17.529022. doi: 10.1101/2023.02.17.529022.
Mass spectrometry data entry: PXD039931
9 Tunable Amine-Reactive Electrophiles for Selective Profiling of Lysine. Angew Chem Int Ed Engl. 2022 Jan 26;61(5):e202112107. doi: 10.1002/anie.202112107. Epub 2021 Dec 16.
10 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
11 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
12 Pharmacological Targeting of Vacuolar H(+)-ATPase via Subunit V1G Combats Multidrug-Resistant Cancer. Cell Chem Biol. 2020 Nov 19;27(11):1359-1370.e8. doi: 10.1016/j.chembiol.2020.06.011. Epub 2020 Jul 9.
13 Site-specific quantitative cysteine profiling with data-independent acquisition-based mass spectrometry. Methods Enzymol. 2023;679:295-322. doi: 10.1016/bs.mie.2022.07.037. Epub 2022 Sep 7.
Mass spectrometry data entry: PXD027578