Details of the Target

General Information of Target

Target ID LDTP04814
Target Name A disintegrin and metalloproteinase with thrombospondin motifs 12 (ADAMTS12)
Gene Name ADAMTS12
Gene ID 81792
Synonyms
A disintegrin and metalloproteinase with thrombospondin motifs 12; ADAM-TS 12; ADAM-TS12; ADAMTS-12; EC 3.4.24.-
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MPCAQRSWLANLSVVAQLLNFGALCYGRQPQPGPVRFPDRRQEHFIKGLPEYHVVGPVRV
DASGHFLSYGLHYPITSSRRKRDLDGSEDWVYYRISHEEKDLFFNLTVNQGFLSNSYIME
KRYGNLSHVKMMASSAPLCHLSGTVLQQGTRVGTAALSACHGLTGFFQLPHGDFFIEPVK
KHPLVEGGYHPHIVYRRQKVPETKEPTCGLKDSVNISQKQELWREKWERHNLPSRSLSRR
SISKERWVETLVVADTKMIEYHGSENVESYILTIMNMVTGLFHNPSIGNAIHIVVVRLIL
LEEEEQGLKIVHHAEKTLSSFCKWQKSINPKSDLNPVHHDVAVLLTRKDICAGFNRPCET
LGLSHLSGMCQPHRSCNINEDSGLPLAFTIAHELGHSFGIQHDGKENDCEPVGRHPYIMS
RQLQYDPTPLTWSKCSEEYITRFLDRGWGFCLDDIPKKKGLKSKVIAPGVIYDVHHQCQL
QYGPNATFCQEVENVCQTLWCSVKGFCRSKLDAAADGTQCGEKKWCMAGKCITVGKKPES
IPGGWGRWSPWSHCSRTCGAGVQSAERLCNNPEPKFGGKYCTGERKRYRLCNVHPCRSEA
PTFRQMQCSEFDTVPYKNELYHWFPIFNPAHPCELYCRPIDGQFSEKMLDAVIDGTPCFE
GGNSRNVCINGICKMVGCDYEIDSNATEDRCGVCLGDGSSCQTVRKMFKQKEGSGYVDIG
LIPKGARDIRVMEIEGAGNFLAIRSEDPEKYYLNGGFIIQWNGNYKLAGTVFQYDRKGDL
EKLMATGPTNESVWIQLLFQVTNPGIKYEYTIQKDGLDNDVEQQMYFWQYGHWTECSVTC
GTGIRRQTAHCIKKGRGMVKATFCDPETQPNGRQKKCHEKACPPRWWAGEWEACSATCGP
HGEKKRTVLCIQTMVSDEQALPPTDCQHLLKPKTLLSCNRDILCPSDWTVGNWSECSVSC
GGGVRIRSVTCAKNHDEPCDVTRKPNSRALCGLQQCPSSRRVLKPNKGTISNGKNPPTLK
PVPPPTSRPRMLTTPTGPESMSTSTPAISSPSPTTASKEGDLGGKQWQDSSTQPELSSRY
LISTGSTSQPILTSQSLSIQPSEENVSSSDTGPTSEGGLVATTTSGSGLSSSRNPITWPV
TPFYNTLTKGPEMEIHSGSGEEREQPEDKDESNPVIWTKIRVPGNDAPVESTEMPLAPPL
TPDLSRESWWPPFSTVMEGLLPSQRPTTSETGTPRVEGMVTEKPANTLLPLGGDHQPEPS
GKTANRNHLKLPNNMNQTKSSEPVLTEEDATSLITEGFLLNASNYKQLTNGHGSAHWIVG
NWSECSTTCGLGAYWRRVECSTQMDSDCAAIQRPDPAKRCHLRPCAGWKVGNWSKCSRNC
SGGFKIREIQCVDSRDHRNLRPFHCQFLAGIPPPLSMSCNPEPCEAWQVEPWSQCSRSCG
GGVQERGVFCPGGLCDWTKRPTSTMSCNEHLCCHWATGNWDLCSTSCGGGFQKRTVQCVP
SEGNKTEDQDQCLCDHKPRPPEFKKCNQQACKKSADLLCTKDKLSASFCQTLKAMKKCSV
PTVRAECCFSCPQTHITHTQRQRRQRLLQKSKEL
Target Bioclass
Enzyme
Subcellular location
Secreted, extracellular space, extracellular matrix
Function Metalloprotease that may play a role in the degradation of COMP. Cleaves also alpha-2 macroglobulin and aggregan. Has anti-tumorigenic properties.
Uniprot ID
P58397
Ensemble ID
ENST00000352040.7
HGNC ID
HGNC:14605

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
C32 SNV: p.G692D .
CAL62 SNV: p.R1266L .
CCK81 SNV: p.L1220V .
DU145 SNV: p.V950L; p.P1431S .
EFO21 SNV: p.Y752C .
FADU SNV: p.D1510N .
FTC133 SNV: p.P1402Q .
G415 SNV: p.N1012D .
HT115 SNV: p.Y752C .
Ishikawa (Heraklio) 02 ER SNV: p.S1292R .
JURKAT Deletion: p.F104LfsTer3 .
KASUMI2 SNV: p.L363M .
KYSE180 SNV: p.Q477Ter .
KYSE30 Substitution: p.E205K .
LN18 SNV: p.I291L .
MDAMB436 Substitution: p.V267G .
MEWO SNV: p.I806L .
MFE319 SNV: p.G27E; p.T1485A .
MIAPACA2 SNV: p.F772L .
NCIH1703 SNV: p.C1391F .
NCIH1792 SNV: p.E245Q .
NCIH2172 SNV: p.G383Ter; p.C979S; p.P1518H; p.P1521T .
NCIH2286 SNV: p.R347T .
NCIH2291 SNV: p.H339Q .
NCIH3122 SNV: p.R597C .
PC9 SNV: p.A849S .
RKO SNV: p.R1387H .
RL952 SNV: p.H1316Q .
SKMEL24 SNV: p.V194A .
SKMM2 SNV: p.K530R .
SKN SNV: p.N1420K .
SW1116 SNV: p.Ter1595LextTer15 .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 2 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C673(0.82)  LDD0532  [1]
VSF
 Probe Info  		N.A.  LDD0007  [2]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0215  AC10 HCT 116 C673(0.82)  LDD0532  [1]
 LDCM0226  AC11 HCT 116 C673(0.87)  LDD0543  [1]
 LDCM0237  AC12 HCT 116 C673(1.09)  LDD0554  [1]
 LDCM0246  AC128 HCT 116 C673(0.80)  LDD0563  [1]
 LDCM0247  AC129 HCT 116 C673(1.43)  LDD0564  [1]
 LDCM0249  AC130 HCT 116 C673(0.87)  LDD0566  [1]
 LDCM0250  AC131 HCT 116 C673(1.25)  LDD0567  [1]
 LDCM0251  AC132 HCT 116 C673(1.32)  LDD0568  [1]
 LDCM0252  AC133 HCT 116 C673(0.98)  LDD0569  [1]
 LDCM0253  AC134 HCT 116 C673(0.90)  LDD0570  [1]
 LDCM0254  AC135 HCT 116 C673(0.85)  LDD0571  [1]
 LDCM0255  AC136 HCT 116 C673(0.99)  LDD0572  [1]
 LDCM0256  AC137 HCT 116 C673(1.09)  LDD0573  [1]
 LDCM0257  AC138 HCT 116 C673(0.91)  LDD0574  [1]
 LDCM0258  AC139 HCT 116 C673(1.04)  LDD0575  [1]
 LDCM0259  AC14 HCT 116 C673(1.06)  LDD0576  [1]
 LDCM0260  AC140 HCT 116 C673(0.89)  LDD0577  [1]
 LDCM0261  AC141 HCT 116 C673(0.98)  LDD0578  [1]
 LDCM0262  AC142 HCT 116 C673(1.90)  LDD0579  [1]
 LDCM0263  AC143 HCT 116 C673(0.77)  LDD0580  [1]
 LDCM0264  AC144 HCT 116 C673(0.45)  LDD0581  [1]
 LDCM0265  AC145 HCT 116 C673(0.61)  LDD0582  [1]
 LDCM0266  AC146 HCT 116 C673(0.59)  LDD0583  [1]
 LDCM0267  AC147 HCT 116 C673(0.48)  LDD0584  [1]
 LDCM0268  AC148 HCT 116 C673(0.48)  LDD0585  [1]
 LDCM0269  AC149 HCT 116 C673(0.45)  LDD0586  [1]
 LDCM0270  AC15 HCT 116 C673(0.82)  LDD0587  [1]
 LDCM0271  AC150 HCT 116 C673(0.69)  LDD0588  [1]
 LDCM0272  AC151 HCT 116 C673(0.76)  LDD0589  [1]
 LDCM0273  AC152 HCT 116 C673(0.44)  LDD0590  [1]
 LDCM0274  AC153 HCT 116 C673(0.59)  LDD0591  [1]
 LDCM0621  AC154 HCT 116 C673(0.51)  LDD2158  [1]
 LDCM0622  AC155 HCT 116 C673(0.49)  LDD2159  [1]
 LDCM0623  AC156 HCT 116 C673(0.97)  LDD2160  [1]
 LDCM0624  AC157 HCT 116 C673(0.91)  LDD2161  [1]
 LDCM0285  AC25 PaTu 8988t C673(0.98)  LDD1164  [1]
 LDCM0286  AC26 PaTu 8988t C673(0.89)  LDD1165  [1]
 LDCM0287  AC27 PaTu 8988t C673(0.78)  LDD1166  [1]
 LDCM0288  AC28 PaTu 8988t C673(0.82)  LDD1167  [1]
 LDCM0289  AC29 PaTu 8988t C673(0.84)  LDD1168  [1]
 LDCM0291  AC30 PaTu 8988t C673(0.85)  LDD1170  [1]
 LDCM0292  AC31 PaTu 8988t C673(0.91)  LDD1171  [1]
 LDCM0293  AC32 PaTu 8988t C673(0.95)  LDD1172  [1]
 LDCM0294  AC33 PaTu 8988t C673(0.93)  LDD1173  [1]
 LDCM0295  AC34 PaTu 8988t C673(0.80)  LDD1174  [1]
 LDCM0320  AC57 PaTu 8988t C673(0.82)  LDD1199  [1]
 LDCM0321  AC58 PaTu 8988t C673(0.87)  LDD1200  [1]
 LDCM0322  AC59 PaTu 8988t C673(0.93)  LDD1201  [1]
 LDCM0323  AC6 HCT 116 C673(0.80)  LDD0640  [1]
 LDCM0324  AC60 PaTu 8988t C673(0.95)  LDD1203  [1]
 LDCM0325  AC61 PaTu 8988t C673(0.87)  LDD1204  [1]
 LDCM0326  AC62 PaTu 8988t C673(0.93)  LDD1205  [1]
 LDCM0327  AC63 PaTu 8988t C673(0.92)  LDD1206  [1]
 LDCM0328  AC64 PaTu 8988t C673(1.06)  LDD1207  [1]
 LDCM0329  AC65 PaTu 8988t C673(1.04)  LDD1208  [1]
 LDCM0330  AC66 PaTu 8988t C673(1.01)  LDD1209  [1]
 LDCM0331  AC67 PaTu 8988t C673(1.02)  LDD1210  [1]
 LDCM0334  AC7 HCT 116 C673(0.89)  LDD0651  [1]
 LDCM0345  AC8 HCT 116 C673(0.91)  LDD0662  [1]
 LDCM0349  AC83 PaTu 8988t C673(1.10)  LDD1228  [1]
 LDCM0350  AC84 PaTu 8988t C673(1.04)  LDD1229  [1]
 LDCM0351  AC85 PaTu 8988t C673(1.15)  LDD1230  [1]
 LDCM0352  AC86 PaTu 8988t C673(1.05)  LDD1231  [1]
 LDCM0353  AC87 PaTu 8988t C673(1.37)  LDD1232  [1]
 LDCM0354  AC88 PaTu 8988t C673(1.27)  LDD1233  [1]
 LDCM0355  AC89 PaTu 8988t C673(1.12)  LDD1234  [1]
 LDCM0357  AC90 PaTu 8988t C673(1.23)  LDD1236  [1]
 LDCM0358  AC91 PaTu 8988t C673(1.27)  LDD1237  [1]
 LDCM0359  AC92 PaTu 8988t C673(1.05)  LDD1238  [1]
 LDCM0360  AC93 PaTu 8988t C673(1.55)  LDD1239  [1]
 LDCM0361  AC94 PaTu 8988t C673(1.19)  LDD1240  [1]
 LDCM0362  AC95 PaTu 8988t C673(1.18)  LDD1241  [1]
 LDCM0363  AC96 PaTu 8988t C673(1.67)  LDD1242  [1]
 LDCM0364  AC97 PaTu 8988t C673(1.28)  LDD1243  [1]
 LDCM0248  AKOS034007472 HCT 116 C673(1.09)  LDD0565  [1]
 LDCM0356  AKOS034007680 HCT 116 C673(0.90)  LDD0673  [1]
 LDCM0275  AKOS034007705 HCT 116 C673(0.75)  LDD0592  [1]
 LDCM0367  CL1 HCT 116 C673(0.92)  LDD0684  [1]
 LDCM0368  CL10 HCT 116 C673(1.03)  LDD0685  [1]
 LDCM0370  CL101 HCT 116 C673(0.83)  LDD0687  [1]
 LDCM0371  CL102 HCT 116 C673(0.99)  LDD0688  [1]
 LDCM0372  CL103 HCT 116 C673(1.03)  LDD0689  [1]
 LDCM0373  CL104 HCT 116 C673(0.99)  LDD0690  [1]
 LDCM0379  CL11 HCT 116 C673(1.21)  LDD0696  [1]
 LDCM0382  CL112 PaTu 8988t C673(1.02)  LDD1261  [1]
 LDCM0383  CL113 PaTu 8988t C673(0.91)  LDD1262  [1]
 LDCM0384  CL114 PaTu 8988t C673(0.92)  LDD1263  [1]
 LDCM0385  CL115 PaTu 8988t C673(0.90)  LDD1264  [1]
 LDCM0386  CL116 PaTu 8988t C673(1.44)  LDD1265  [1]
 LDCM0390  CL12 HCT 116 C673(1.16)  LDD0707  [1]
 LDCM0396  CL125 PaTu 8988t C673(0.98)  LDD1275  [1]
 LDCM0397  CL126 PaTu 8988t C673(1.02)  LDD1276  [1]
 LDCM0398  CL127 PaTu 8988t C673(0.89)  LDD1277  [1]
 LDCM0399  CL128 PaTu 8988t C673(0.94)  LDD1278  [1]
 LDCM0400  CL13 HCT 116 C673(0.98)  LDD0717  [1]
 LDCM0401  CL14 HCT 116 C673(0.95)  LDD0718  [1]
 LDCM0402  CL15 HCT 116 C673(1.10)  LDD0719  [1]
 LDCM0407  CL2 HCT 116 C673(0.91)  LDD0724  [1]
 LDCM0418  CL3 HCT 116 C673(0.87)  LDD0735  [1]
 LDCM0429  CL4 HCT 116 C673(0.81)  LDD0746  [1]
 LDCM0436  CL46 HCT 116 C673(1.39)  LDD0753  [1]
 LDCM0437  CL47 HCT 116 C673(1.29)  LDD0754  [1]
 LDCM0438  CL48 HCT 116 C673(1.44)  LDD0755  [1]
 LDCM0439  CL49 HCT 116 C673(1.04)  LDD0756  [1]
 LDCM0440  CL5 HCT 116 C673(0.97)  LDD0757  [1]
 LDCM0441  CL50 HCT 116 C673(1.14)  LDD0758  [1]
 LDCM0442  CL51 HCT 116 C673(1.21)  LDD0759  [1]
 LDCM0443  CL52 HCT 116 C673(1.23)  LDD0760  [1]
 LDCM0444  CL53 HCT 116 C673(1.13)  LDD0761  [1]
 LDCM0445  CL54 HCT 116 C673(1.22)  LDD0762  [1]
 LDCM0446  CL55 HCT 116 C673(1.30)  LDD0763  [1]
 LDCM0447  CL56 HCT 116 C673(1.09)  LDD0764  [1]
 LDCM0448  CL57 HCT 116 C673(1.15)  LDD0765  [1]
 LDCM0449  CL58 HCT 116 C673(1.45)  LDD0766  [1]
 LDCM0450  CL59 HCT 116 C673(1.11)  LDD0767  [1]
 LDCM0451  CL6 HCT 116 C673(1.23)  LDD0768  [1]
 LDCM0452  CL60 HCT 116 C673(1.06)  LDD0769  [1]
 LDCM0453  CL61 HCT 116 C673(0.93)  LDD0770  [1]
 LDCM0454  CL62 HCT 116 C673(1.00)  LDD0771  [1]
 LDCM0455  CL63 HCT 116 C673(0.93)  LDD0772  [1]
 LDCM0456  CL64 HCT 116 C673(0.68)  LDD0773  [1]
 LDCM0457  CL65 HCT 116 C673(0.82)  LDD0774  [1]
 LDCM0458  CL66 HCT 116 C673(0.77)  LDD0775  [1]
 LDCM0459  CL67 HCT 116 C673(0.88)  LDD0776  [1]
 LDCM0460  CL68 HCT 116 C673(1.13)  LDD0777  [1]
 LDCM0461  CL69 HCT 116 C673(1.12)  LDD0778  [1]
 LDCM0462  CL7 HCT 116 C673(1.01)  LDD0779  [1]
 LDCM0463  CL70 HCT 116 C673(1.13)  LDD0780  [1]
 LDCM0464  CL71 HCT 116 C673(0.85)  LDD0781  [1]
 LDCM0465  CL72 HCT 116 C673(0.90)  LDD0782  [1]
 LDCM0466  CL73 HCT 116 C673(0.86)  LDD0783  [1]
 LDCM0467  CL74 HCT 116 C673(0.77)  LDD0784  [1]
 LDCM0473  CL8 HCT 116 C673(0.92)  LDD0790  [1]
 LDCM0484  CL9 HCT 116 C673(0.98)  LDD0801  [1]
 LDCM0468  Fragment33 HCT 116 C673(0.96)  LDD0785  [1]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Tripartite motif-containing protein 14 (TRIM14) TRIM/RBCC family Q14142
Transcription factor
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Homeobox protein MOX-2 (MEOX2) . P50222
Other
Click To Hide/Show 4 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Coilin (COIL) Coilin family P38432
Cartilage oligomeric matrix protein (COMP) Thrombospondin family P49747
Interactor of HORMAD1 protein 1 (IHO1) . Q8IYA8
Myosin light polypeptide 6 (MYL6) . P60660

References

1 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
2 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764