Details of the Target

General Information of Target

Target ID LDTP04635
Target Name Galactocerebrosidase (GALC)
Gene Name GALC
Gene ID 2581
Synonyms
Galactocerebrosidase; GALCERase; EC 3.2.1.46; Galactocerebroside beta-galactosidase; Galactosylceramidase; Galactosylceramide beta-galactosidase
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MAEWLLSASWQRRAKAMTAAAGSAGRAAVPLLLCALLAPGGAYVLDDSDGLGREFDGIGA
VSGGGATSRLLVNYPEPYRSQILDYLFKPNFGASLHILKVEIGGDGQTTDGTEPSHMHYA
LDENYFRGYEWWLMKEAKKRNPNITLIGLPWSFPGWLGKGFDWPYVNLQLTAYYVVTWIV
GAKRYHDLDIDYIGIWNERSYNANYIKILRKMLNYQGLQRVKIIASDNLWESISASMLLD
AELFKVVDVIGAHYPGTHSAKDAKLTGKKLWSSEDFSTLNSDMGAGCWGRILNQNYINGY
MTSTIAWNLVASYYEQLPYGRCGLMTAQEPWSGHYVVESPVWVSAHTTQFTQPGWYYLKT
VGHLEKGGSYVALTDGLGNLTIIIETMSHKHSKCIRPFLPYFNVSQQFATFVLKGSFSEI
PELQVWYTKLGKTSERFLFKQLDSLWLLDSDGSFTLSLHEDELFTLTTLTTGRKGSYPLP
PKSQPFPSTYKDDFNVDYPFFSEAPNFADQTGVFEYFTNIEDPGEHHFTLRQVLNQRPIT
WAADASNTISIIGDYNWTNLTIKCDVYIETPDTGGVFIAGRVNKGGILIRSARGIFFWIF
ANGSYRVTGDLAGWIIYALGRVEVTAKKWYTLTLTIKGHFTSGMLNDKSLWTDIPVNFPK
NGWAAIGTHSFEFAQFDNFLVEATR
Target Type
Clinical trial
Target Bioclass
Enzyme
Family
Glycosyl hydrolase 59 family
Subcellular location
Lysosome
Function
Hydrolyzes the galactose ester bonds of glycolipids such as galactosylceramide and galactosylsphingosine. Enzyme with very low activity responsible for the lysosomal catabolism of galactosylceramide, a major lipid in myelin, kidney and epithelial cells of small intestine and colon.
TTD ID
T72024
Uniprot ID
P54803
DrugMap ID
TT5IZRB
Ensemble ID
ENST00000261304.7
HGNC ID
HGNC:4115
ChEMBL ID
CHEMBL3713095

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
DAOY SNV: p.S416Y .
EFO27 Insertion: p.A601CfsTer10 .
GAMG SNV: p.I102V .
HCC1806 SNV: p.T652K .
JEKO1 SNV: p.Y173D .
LS180 Deletion: p.K628NfsTer7 .
MCC13 SNV: p.G367R .
SUDHL6 SNV: p.T455I .
SW1271 SNV: p.L6P .
TOV21G Deletion: p.K628NfsTer7 .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 4 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
FBPP2
 Probe Info  		6.21  LDD0318  [1]
Probe 1
 Probe Info  		Y215(22.61)  LDD3495  [2]
DBIA
 Probe Info  		C287(0.81)  LDD1093  [3]
NAIA_5
 Probe Info  		N.A.  LDD2223  [4]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0214  AC1 PaTu 8988t C287(0.81)  LDD1093  [3]
 LDCM0215  AC10 PaTu 8988t C287(1.13)  LDD1094  [3]
 LDCM0226  AC11 PaTu 8988t C287(1.04)  LDD1105  [3]
 LDCM0237  AC12 PaTu 8988t C287(0.97)  LDD1116  [3]
 LDCM0259  AC14 PaTu 8988t C287(1.19)  LDD1138  [3]
 LDCM0270  AC15 PaTu 8988t C287(1.16)  LDD1149  [3]
 LDCM0276  AC17 PaTu 8988t C287(0.67)  LDD1155  [3]
 LDCM0277  AC18 PaTu 8988t C287(1.03)  LDD1156  [3]
 LDCM0278  AC19 PaTu 8988t C287(0.87)  LDD1157  [3]
 LDCM0279  AC2 PaTu 8988t C287(0.56)  LDD1158  [3]
 LDCM0280  AC20 PaTu 8988t C287(1.50)  LDD1159  [3]
 LDCM0281  AC21 PaTu 8988t C287(0.83)  LDD1160  [3]
 LDCM0282  AC22 PaTu 8988t C287(0.86)  LDD1161  [3]
 LDCM0283  AC23 PaTu 8988t C287(0.87)  LDD1162  [3]
 LDCM0284  AC24 PaTu 8988t C287(0.73)  LDD1163  [3]
 LDCM0285  AC25 PaTu 8988t C287(1.05)  LDD1164  [3]
 LDCM0286  AC26 PaTu 8988t C287(0.80)  LDD1165  [3]
 LDCM0287  AC27 PaTu 8988t C287(1.26)  LDD1166  [3]
 LDCM0288  AC28 PaTu 8988t C287(1.55)  LDD1167  [3]
 LDCM0289  AC29 PaTu 8988t C287(1.41)  LDD1168  [3]
 LDCM0290  AC3 PaTu 8988t C287(0.68)  LDD1169  [3]
 LDCM0291  AC30 PaTu 8988t C287(1.58)  LDD1170  [3]
 LDCM0292  AC31 PaTu 8988t C287(1.40)  LDD1171  [3]
 LDCM0293  AC32 PaTu 8988t C287(1.01)  LDD1172  [3]
 LDCM0294  AC33 PaTu 8988t C287(1.30)  LDD1173  [3]
 LDCM0295  AC34 PaTu 8988t C287(1.01)  LDD1174  [3]
 LDCM0296  AC35 PaTu 8988t C287(0.96)  LDD1175  [3]
 LDCM0297  AC36 PaTu 8988t C287(1.04)  LDD1176  [3]
 LDCM0298  AC37 PaTu 8988t C287(1.45)  LDD1177  [3]
 LDCM0299  AC38 PaTu 8988t C287(1.26)  LDD1178  [3]
 LDCM0300  AC39 PaTu 8988t C287(1.20)  LDD1179  [3]
 LDCM0301  AC4 PaTu 8988t C287(0.52)  LDD1180  [3]
 LDCM0302  AC40 PaTu 8988t C287(1.24)  LDD1181  [3]
 LDCM0303  AC41 PaTu 8988t C287(1.13)  LDD1182  [3]
 LDCM0304  AC42 PaTu 8988t C287(1.20)  LDD1183  [3]
 LDCM0305  AC43 PaTu 8988t C287(1.00)  LDD1184  [3]
 LDCM0306  AC44 PaTu 8988t C287(1.44)  LDD1185  [3]
 LDCM0307  AC45 PaTu 8988t C287(1.52)  LDD1186  [3]
 LDCM0312  AC5 PaTu 8988t C287(0.71)  LDD1191  [3]
 LDCM0323  AC6 PaTu 8988t C287(1.11)  LDD1202  [3]
 LDCM0334  AC7 PaTu 8988t C287(0.87)  LDD1213  [3]
 LDCM0345  AC8 PaTu 8988t C287(0.82)  LDD1224  [3]
 LDCM0349  AC83 PaTu 8988t C287(1.14)  LDD1228  [3]
 LDCM0350  AC84 PaTu 8988t C287(1.14)  LDD1229  [3]
 LDCM0351  AC85 PaTu 8988t C287(0.79)  LDD1230  [3]
 LDCM0352  AC86 PaTu 8988t C287(0.97)  LDD1231  [3]
 LDCM0353  AC87 PaTu 8988t C287(1.21)  LDD1232  [3]
 LDCM0354  AC88 PaTu 8988t C287(0.81)  LDD1233  [3]
 LDCM0355  AC89 PaTu 8988t C287(0.88)  LDD1234  [3]
 LDCM0357  AC90 PaTu 8988t C287(0.98)  LDD1236  [3]
 LDCM0358  AC91 PaTu 8988t C287(1.09)  LDD1237  [3]
 LDCM0359  AC92 PaTu 8988t C287(1.41)  LDD1238  [3]
 LDCM0360  AC93 PaTu 8988t C287(1.31)  LDD1239  [3]
 LDCM0361  AC94 PaTu 8988t C287(0.80)  LDD1240  [3]
 LDCM0362  AC95 PaTu 8988t C287(1.04)  LDD1241  [3]
 LDCM0363  AC96 PaTu 8988t C287(1.06)  LDD1242  [3]
 LDCM0364  AC97 PaTu 8988t C287(1.10)  LDD1243  [3]
 LDCM0248  AKOS034007472 PaTu 8988t C287(1.25)  LDD1127  [3]
 LDCM0356  AKOS034007680 PaTu 8988t C287(1.01)  LDD1235  [3]
 LDCM0275  AKOS034007705 PaTu 8988t C287(1.16)  LDD1154  [3]
 LDCM0367  CL1 PaTu 8988t C287(1.08)  LDD1246  [3]
 LDCM0368  CL10 PaTu 8988t C287(1.44)  LDD1247  [3]
 LDCM0369  CL100 PaTu 8988t C287(0.59)  LDD1248  [3]
 LDCM0370  CL101 PaTu 8988t C287(0.86)  LDD1249  [3]
 LDCM0371  CL102 PaTu 8988t C287(1.03)  LDD1250  [3]
 LDCM0372  CL103 PaTu 8988t C287(1.04)  LDD1251  [3]
 LDCM0373  CL104 PaTu 8988t C287(1.24)  LDD1252  [3]
 LDCM0374  CL105 PaTu 8988t C287(1.16)  LDD1253  [3]
 LDCM0375  CL106 PaTu 8988t C287(0.78)  LDD1254  [3]
 LDCM0376  CL107 PaTu 8988t C287(0.69)  LDD1255  [3]
 LDCM0377  CL108 PaTu 8988t C287(1.07)  LDD1256  [3]
 LDCM0378  CL109 PaTu 8988t C287(0.70)  LDD1257  [3]
 LDCM0379  CL11 PaTu 8988t C287(1.83)  LDD1258  [3]
 LDCM0380  CL110 PaTu 8988t C287(1.06)  LDD1259  [3]
 LDCM0381  CL111 PaTu 8988t C287(1.42)  LDD1260  [3]
 LDCM0382  CL112 PaTu 8988t C287(1.09)  LDD1261  [3]
 LDCM0383  CL113 PaTu 8988t C287(1.06)  LDD1262  [3]
 LDCM0384  CL114 PaTu 8988t C287(1.98)  LDD1263  [3]
 LDCM0385  CL115 PaTu 8988t C287(0.99)  LDD1264  [3]
 LDCM0386  CL116 PaTu 8988t C287(1.17)  LDD1265  [3]
 LDCM0387  CL117 PaTu 8988t C287(1.13)  LDD1266  [3]
 LDCM0388  CL118 PaTu 8988t C287(1.19)  LDD1267  [3]
 LDCM0389  CL119 PaTu 8988t C287(1.30)  LDD1268  [3]
 LDCM0390  CL12 PaTu 8988t C287(1.98)  LDD1269  [3]
 LDCM0391  CL120 PaTu 8988t C287(1.29)  LDD1270  [3]
 LDCM0400  CL13 PaTu 8988t C287(1.62)  LDD1279  [3]
 LDCM0401  CL14 PaTu 8988t C287(1.42)  LDD1280  [3]
 LDCM0402  CL15 PaTu 8988t C287(2.97)  LDD1281  [3]
 LDCM0403  CL16 PaTu 8988t C287(1.63)  LDD1282  [3]
 LDCM0404  CL17 PaTu 8988t C287(1.39)  LDD1283  [3]
 LDCM0405  CL18 PaTu 8988t C287(6.27)  LDD1284  [3]
 LDCM0406  CL19 PaTu 8988t C287(1.61)  LDD1285  [3]
 LDCM0407  CL2 PaTu 8988t C287(0.98)  LDD1286  [3]
 LDCM0408  CL20 PaTu 8988t C287(0.97)  LDD1287  [3]
 LDCM0409  CL21 PaTu 8988t C287(1.08)  LDD1288  [3]
 LDCM0410  CL22 PaTu 8988t C287(2.02)  LDD1289  [3]
 LDCM0411  CL23 PaTu 8988t C287(1.76)  LDD1290  [3]
 LDCM0412  CL24 PaTu 8988t C287(2.43)  LDD1291  [3]
 LDCM0413  CL25 PaTu 8988t C287(1.80)  LDD1292  [3]
 LDCM0414  CL26 PaTu 8988t C287(1.26)  LDD1293  [3]
 LDCM0415  CL27 PaTu 8988t C287(1.56)  LDD1294  [3]
 LDCM0416  CL28 PaTu 8988t C287(0.97)  LDD1295  [3]
 LDCM0417  CL29 PaTu 8988t C287(0.90)  LDD1296  [3]
 LDCM0418  CL3 PaTu 8988t C287(1.56)  LDD1297  [3]
 LDCM0419  CL30 PaTu 8988t C287(1.83)  LDD1298  [3]
 LDCM0420  CL31 PaTu 8988t C287(1.59)  LDD1299  [3]
 LDCM0421  CL32 PaTu 8988t C287(1.17)  LDD1300  [3]
 LDCM0422  CL33 PaTu 8988t C287(1.50)  LDD1301  [3]
 LDCM0423  CL34 PaTu 8988t C287(1.42)  LDD1302  [3]
 LDCM0424  CL35 PaTu 8988t C287(1.43)  LDD1303  [3]
 LDCM0425  CL36 PaTu 8988t C287(0.85)  LDD1304  [3]
 LDCM0426  CL37 PaTu 8988t C287(1.45)  LDD1305  [3]
 LDCM0428  CL39 PaTu 8988t C287(1.23)  LDD1307  [3]
 LDCM0429  CL4 PaTu 8988t C287(1.05)  LDD1308  [3]
 LDCM0430  CL40 PaTu 8988t C287(1.22)  LDD1309  [3]
 LDCM0431  CL41 PaTu 8988t C287(1.18)  LDD1310  [3]
 LDCM0432  CL42 PaTu 8988t C287(1.49)  LDD1311  [3]
 LDCM0433  CL43 PaTu 8988t C287(1.36)  LDD1312  [3]
 LDCM0434  CL44 PaTu 8988t C287(1.74)  LDD1313  [3]
 LDCM0435  CL45 PaTu 8988t C287(1.81)  LDD1314  [3]
 LDCM0436  CL46 PaTu 8988t C287(1.51)  LDD1315  [3]
 LDCM0437  CL47 PaTu 8988t C287(1.86)  LDD1316  [3]
 LDCM0438  CL48 PaTu 8988t C287(1.28)  LDD1317  [3]
 LDCM0439  CL49 PaTu 8988t C287(2.21)  LDD1318  [3]
 LDCM0440  CL5 PaTu 8988t C287(1.71)  LDD1319  [3]
 LDCM0441  CL50 PaTu 8988t C287(1.33)  LDD1320  [3]
 LDCM0442  CL51 PaTu 8988t C287(1.66)  LDD1321  [3]
 LDCM0443  CL52 PaTu 8988t C287(2.78)  LDD1322  [3]
 LDCM0444  CL53 PaTu 8988t C287(1.48)  LDD1323  [3]
 LDCM0445  CL54 PaTu 8988t C287(1.38)  LDD1324  [3]
 LDCM0446  CL55 PaTu 8988t C287(1.41)  LDD1325  [3]
 LDCM0447  CL56 PaTu 8988t C287(0.68)  LDD1326  [3]
 LDCM0448  CL57 PaTu 8988t C287(1.60)  LDD1327  [3]
 LDCM0449  CL58 PaTu 8988t C287(1.42)  LDD1328  [3]
 LDCM0450  CL59 PaTu 8988t C287(1.23)  LDD1329  [3]
 LDCM0451  CL6 PaTu 8988t C287(1.53)  LDD1330  [3]
 LDCM0452  CL60 PaTu 8988t C287(1.64)  LDD1331  [3]
 LDCM0462  CL7 PaTu 8988t C287(1.48)  LDD1341  [3]
 LDCM0473  CL8 PaTu 8988t C287(1.37)  LDD1352  [3]
 LDCM0484  CL9 PaTu 8988t C287(1.82)  LDD1363  [3]
 LDCM0486  CL91 PaTu 8988t C287(0.56)  LDD1365  [3]
 LDCM0487  CL92 PaTu 8988t C287(0.64)  LDD1366  [3]
 LDCM0488  CL93 PaTu 8988t C287(0.52)  LDD1367  [3]
 LDCM0489  CL94 PaTu 8988t C287(0.65)  LDD1368  [3]
 LDCM0490  CL95 PaTu 8988t C287(0.73)  LDD1369  [3]
 LDCM0491  CL96 PaTu 8988t C287(0.73)  LDD1370  [3]
 LDCM0492  CL97 PaTu 8988t C287(0.60)  LDD1371  [3]
 LDCM0493  CL98 PaTu 8988t C287(0.72)  LDD1372  [3]
 LDCM0494  CL99 PaTu 8988t C287(0.84)  LDD1373  [3]
 LDCM0427  Fragment51 PaTu 8988t C287(1.37)  LDD1306  [3]

References

1 Tranylcypromine specificity for monoamine oxidase is limited by promiscuous protein labelling and lysosomal trapping. RSC Chem Biol. 2020 Aug 12;1(4):209-213. doi: 10.1039/d0cb00048e. eCollection 2020 Oct 1.
Mass spectrometry data entry: PXD018580
2 An Azo Coupling-Based Chemoproteomic Approach to Systematically Profile the Tyrosine Reactivity in the Human Proteome. Anal Chem. 2021 Jul 27;93(29):10334-10342. doi: 10.1021/acs.analchem.1c01935. Epub 2021 Jul 12.
3 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
4 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264