Details of the Target

General Information of Target

Target ID LDTP03483
Target Name AT-rich interactive domain-containing protein 4A (ARID4A)
Gene Name ARID4A
Gene ID 5926
Synonyms
RBBP1; RBP1; AT-rich interactive domain-containing protein 4A; ARID domain-containing protein 4A; Retinoblastoma-binding protein 1; RBBP-1
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MKAADEPAYLTVGTDVSAKYRGAFCEAKIKTVKRLVKVKVLLKQDNTTQLVQDDQVKGPL
RVGAIVETRTSDGSFQEAIISKLTDASWYTVVFDDGDERTLRRTSLCLKGERHFAESETL
DQLPLTNPEHFGTPVIAKKTNRGRRSSLPVTEDEKEEESSEEEDEDKRRLNDELLGKVVS
VVSATERTEWYPALVISPSCNDDITVKKDQCLVRSFIDSKFYSIARKDIKEVDILNLPES
ELSTKPGLQKASIFLKTRVVPDNWKMDISEILESSSSDDEDGPAEENDEEKEKEAKKTEE
EVPEEELDPEERDNFLQQLYKFMEDRGTPINKPPVLGYKDLNLFKLFRLVYHQGGCDNID
SGAVWKQIYMDLGIPILNSAASYNVKTAYRKYLYGFEEYCRSANIQFRTVHHHEPKVKEE
KKDLEESMEEALKLDQEMPLTEVKSEPEENIDSNSESEREEIELKSPRGRRRIARDVNSI
KKEIEEEKTEDKLKDNDTENKDVDDDYETAEKKENELLLGRKNTPKQKEKKIKKQEDSDK
DSDEEEEKSQEREETESKCDSEGEEDEEDMEPCLTGTKVKVKYGRGKTQKIYEASIKSTE
IDDGEVLYLVHYYGWNVRYDEWVKADRIIWPLDKGGPKKKQKKKAKNKEDSEKDEKRDEE
RQKSKRGRPPLKSTLSSNMPYGLSKTANSEGKSDSCSSDSETEDALEKNLINEELSLKDE
LEKNENLNDDKLDEENPKISAHILKENDRTQMQPLETLKLEVGENEQIVQIFGNKMEKTE
EVKKEAEKSPKGKGRRSKTKDLSLEIIKISSFGQNEAGSEPHIEAHSLELSSLDNKNFSS
ATEDEIDQCVKEKKLKRKILGQSSPEKKIRIENGMEMTNTVSQERTSDCIGSEGMKNLNF
EQHFERENEGMPSLIAESNQCIQQLTSERFDSPAEETVNIPLKEDEDAMPLIGPETLVCH
EVDLDDLDEKDKTSIEDVAVESSESNSLVSIPPALPPVVQHNFSVASPLTLSQDESRSVK
SESDITIEVDSIAEESQEGLCERESANGFETNVASGTCSIIVQERESREKGQKRPSDGNS
GLMAKKQKRTPKRTSAAAKNEKNGTGQSSDSEDLPVLDNSSKCTPVKHLNVSKPQKLARS
PARISPHIKDGEKDKHREKHPNSSPRTYKWSFQLNELDNMNSTERISFLQEKLQEIRKYY
MSLKSEVATIDRRRKRLKKKDREVSHAGASMSSASSDTGMSPSSSSPPQNVLAVECR
Target Bioclass
Transcription factor
Subcellular location
Nucleus
Function
DNA-binding protein which modulates activity of several transcription factors including RB1 (retinoblastoma-associated protein) and AR (androgen receptor). May function as part of an mSin3A repressor complex. Has no intrinsic transcriptional activity. Plays a role in the regulation of epigenetic modifications at the PWS/AS imprinting center near the SNRPN promoter, where it might function as part of a complex with RB1 and ARID4B. Involved in spermatogenesis, together with ARID4B, where it acts as a transcriptional coactivator for AR and enhances expression of genes required for sperm maturation. Regulates expression of the tight junction protein CLDN3 in the testis, which is important for integrity of the blood-testis barrier. Plays a role in myeloid homeostasis where it regulates the histone methylation state of bone marrow cells and expression of various genes involved in hematopoiesis. May function as a leukemia suppressor.
Uniprot ID
P29374
Ensemble ID
ENST00000348476.7
HGNC ID
HGNC:9885
ChEMBL ID
CHEMBL3709855

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
CCK81 SNV: p.N515I .
COLO320 SNV: p.D1077N DBIA    Probe Info 
HSC3 SNV: p.D650H .
HT115 SNV: p.R521I DBIA    Probe Info 
KMCH1 SNV: p.D1118E .
OVK18 Deletion: p.A645LfsTer27 .
REH Substitution: p.P375V .
RKO Deletion: p.D423TfsTer2 DBIA    Probe Info 
RL952 SNV: p.A1098T .
YSCCC SNV: p.R1143K DBIA    Probe Info 

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 8 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
IPM
 Probe Info  		N.A.  LDD0241  [1]
Probe 1
 Probe Info  		Y592(8.74)  LDD3495  [2]
m-APA
 Probe Info  		14.08  LDD0403  [3]
DBIA
 Probe Info  		C559(1.66); C573(1.66)  LDD0080  [4]
4-Iodoacetamidophenylacetylene
 Probe Info  		N.A.  LDD0038  [5]
IA-alkyne
 Probe Info  		C25(0.00); C400(0.00)  LDD0036  [5]
Lodoacetamide azide
 Probe Info  		N.A.  LDD0037  [5]
NAIA_5
 Probe Info  		N.A.  LDD2223  [6]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0215  AC10 HEK-293T C889(1.14)  LDD1508  [7]
 LDCM0226  AC11 HEK-293T C356(0.99)  LDD1509  [7]
 LDCM0237  AC12 HEK-293T C356(1.15)  LDD1510  [7]
 LDCM0259  AC14 HEK-293T C356(1.28)  LDD1512  [7]
 LDCM0270  AC15 HEK-293T C356(1.11); C959(1.10)  LDD1513  [7]
 LDCM0277  AC18 HEK-293T C889(1.03)  LDD1516  [7]
 LDCM0278  AC19 HEK-293T C356(1.02)  LDD1517  [7]
 LDCM0279  AC2 HEK-293T C889(1.06)  LDD1518  [7]
 LDCM0280  AC20 HEK-293T C356(1.83)  LDD1519  [7]
 LDCM0281  AC21 HEK-293T C356(1.18)  LDD1520  [7]
 LDCM0282  AC22 HEK-293T C356(1.20)  LDD1521  [7]
 LDCM0283  AC23 HEK-293T C356(1.32); C959(0.98)  LDD1522  [7]
 LDCM0284  AC24 HEK-293T C356(1.09)  LDD1523  [7]
 LDCM0286  AC26 HEK-293T C889(1.05)  LDD1525  [7]
 LDCM0287  AC27 HEK-293T C356(0.98)  LDD1526  [7]
 LDCM0288  AC28 HEK-293T C356(0.93)  LDD1527  [7]
 LDCM0289  AC29 HEK-293T C356(1.21)  LDD1528  [7]
 LDCM0290  AC3 HEK-293T C356(1.00)  LDD1529  [7]
 LDCM0291  AC30 HEK-293T C356(0.86)  LDD1530  [7]
 LDCM0292  AC31 HEK-293T C356(1.77); C959(1.10)  LDD1531  [7]
 LDCM0293  AC32 HEK-293T C356(1.09)  LDD1532  [7]
 LDCM0295  AC34 HEK-293T C889(0.99)  LDD1534  [7]
 LDCM0296  AC35 HEK-293T C356(1.06)  LDD1535  [7]
 LDCM0297  AC36 HEK-293T C356(1.09)  LDD1536  [7]
 LDCM0298  AC37 HEK-293T C356(1.04)  LDD1537  [7]
 LDCM0299  AC38 HEK-293T C356(1.04)  LDD1538  [7]
 LDCM0300  AC39 HEK-293T C356(1.51); C959(1.05)  LDD1539  [7]
 LDCM0301  AC4 HEK-293T C356(1.21)  LDD1540  [7]
 LDCM0302  AC40 HEK-293T C356(1.00)  LDD1541  [7]
 LDCM0304  AC42 HEK-293T C889(1.09)  LDD1543  [7]
 LDCM0305  AC43 HEK-293T C356(0.98)  LDD1544  [7]
 LDCM0306  AC44 HEK-293T C356(1.19)  LDD1545  [7]
 LDCM0307  AC45 HEK-293T C356(1.04)  LDD1546  [7]
 LDCM0308  AC46 HEK-293T C356(1.03)  LDD1547  [7]
 LDCM0309  AC47 HEK-293T C356(1.28); C959(1.11)  LDD1548  [7]
 LDCM0310  AC48 HEK-293T C356(1.06)  LDD1549  [7]
 LDCM0312  AC5 HEK-293T C356(0.99)  LDD1551  [7]
 LDCM0313  AC50 HEK-293T C889(1.10)  LDD1552  [7]
 LDCM0314  AC51 HEK-293T C356(1.01)  LDD1553  [7]
 LDCM0315  AC52 HEK-293T C356(1.51)  LDD1554  [7]
 LDCM0316  AC53 HEK-293T C356(1.01)  LDD1555  [7]
 LDCM0317  AC54 HEK-293T C356(1.08)  LDD1556  [7]
 LDCM0318  AC55 HEK-293T C356(1.12); C959(1.41)  LDD1557  [7]
 LDCM0319  AC56 HEK-293T C356(1.10)  LDD1558  [7]
 LDCM0321  AC58 HEK-293T C889(1.14)  LDD1560  [7]
 LDCM0322  AC59 HEK-293T C356(1.06)  LDD1561  [7]
 LDCM0323  AC6 HEK-293T C356(1.23)  LDD1562  [7]
 LDCM0324  AC60 HEK-293T C356(1.19)  LDD1563  [7]
 LDCM0325  AC61 HEK-293T C356(1.15)  LDD1564  [7]
 LDCM0326  AC62 HEK-293T C356(0.84)  LDD1565  [7]
 LDCM0327  AC63 HEK-293T C356(1.23); C959(1.09)  LDD1566  [7]
 LDCM0328  AC64 HEK-293T C356(1.13)  LDD1567  [7]
 LDCM0334  AC7 HEK-293T C356(1.27); C959(1.71)  LDD1568  [7]
 LDCM0345  AC8 HEK-293T C356(1.15)  LDD1569  [7]
 LDCM0248  AKOS034007472 HEK-293T C356(1.13)  LDD1511  [7]
 LDCM0275  AKOS034007705 HEK-293T C356(1.09)  LDD1514  [7]
 LDCM0156  Aniline NCI-H1299 14.08  LDD0403  [3]
 LDCM0632  CL-Sc Hep-G2 C889(20.00)  LDD2227  [6]
 LDCM0368  CL10 HEK-293T C356(0.91)  LDD1572  [7]
 LDCM0369  CL100 HEK-293T C356(0.80); C889(1.11)  LDD1573  [7]
 LDCM0371  CL102 HEK-293T C356(1.04)  LDD1575  [7]
 LDCM0373  CL104 HEK-293T C356(0.93); C889(1.02)  LDD1577  [7]
 LDCM0375  CL106 HEK-293T C356(0.96)  LDD1579  [7]
 LDCM0377  CL108 HEK-293T C356(0.95); C889(0.91)  LDD1581  [7]
 LDCM0379  CL11 HEK-293T C356(1.06); C959(1.13)  LDD1583  [7]
 LDCM0380  CL110 HEK-293T C356(0.94)  LDD1584  [7]
 LDCM0382  CL112 HEK-293T C356(0.94); C889(1.02)  LDD1586  [7]
 LDCM0384  CL114 HEK-293T C356(0.85)  LDD1588  [7]
 LDCM0386  CL116 HEK-293T C356(0.85); C889(1.06)  LDD1590  [7]
 LDCM0388  CL118 HEK-293T C356(1.48)  LDD1592  [7]
 LDCM0390  CL12 HEK-293T C356(1.12)  LDD1594  [7]
 LDCM0391  CL120 HEK-293T C356(0.93); C889(0.97)  LDD1595  [7]
 LDCM0393  CL122 HEK-293T C356(0.92)  LDD1597  [7]
 LDCM0395  CL124 HEK-293T C356(0.91); C889(1.01)  LDD1599  [7]
 LDCM0397  CL126 HEK-293T C356(0.73)  LDD1601  [7]
 LDCM0399  CL128 HEK-293T C356(0.98); C889(0.93)  LDD1603  [7]
 LDCM0401  CL14 HEK-293T C356(1.30)  LDD1605  [7]
 LDCM0403  CL16 HEK-293T C356(0.89); C889(1.10)  LDD1607  [7]
 LDCM0405  CL18 HEK-293T C889(1.05)  LDD1609  [7]
 LDCM0406  CL19 HEK-293T C356(1.03)  LDD1610  [7]
 LDCM0407  CL2 HEK-293T C356(0.83)  LDD1611  [7]
 LDCM0408  CL20 HEK-293T C356(1.23)  LDD1612  [7]
 LDCM0409  CL21 HEK-293T C356(0.89)  LDD1613  [7]
 LDCM0410  CL22 HEK-293T C356(0.90)  LDD1614  [7]
 LDCM0411  CL23 HEK-293T C356(1.49); C959(0.66)  LDD1615  [7]
 LDCM0412  CL24 HEK-293T C356(1.19)  LDD1616  [7]
 LDCM0414  CL26 HEK-293T C356(1.09)  LDD1618  [7]
 LDCM0416  CL28 HEK-293T C356(0.83); C889(0.97)  LDD1620  [7]
 LDCM0419  CL30 HEK-293T C889(1.11)  LDD1623  [7]
 LDCM0420  CL31 HEK-293T C356(0.98)  LDD1624  [7]
 LDCM0421  CL32 HEK-293T C356(1.19)  LDD1625  [7]
 LDCM0422  CL33 HEK-293T C356(0.86)  LDD1626  [7]
 LDCM0423  CL34 HEK-293T C356(1.00)  LDD1627  [7]
 LDCM0424  CL35 HEK-293T C356(1.33); C959(0.63)  LDD1628  [7]
 LDCM0425  CL36 HEK-293T C356(1.09)  LDD1629  [7]
 LDCM0429  CL4 HEK-293T C356(0.89); C889(1.00)  LDD1633  [7]
 LDCM0430  CL40 HEK-293T C356(0.95); C889(1.08)  LDD1634  [7]
 LDCM0432  CL42 HEK-293T C889(0.99)  LDD1636  [7]
 LDCM0433  CL43 HEK-293T C356(0.99)  LDD1637  [7]
 LDCM0434  CL44 HEK-293T C356(1.13)  LDD1638  [7]
 LDCM0435  CL45 HEK-293T C356(0.86)  LDD1639  [7]
 LDCM0436  CL46 HEK-293T C356(1.16)  LDD1640  [7]
 LDCM0437  CL47 HEK-293T C356(1.11); C959(0.65)  LDD1641  [7]
 LDCM0438  CL48 HEK-293T C356(1.21)  LDD1642  [7]
 LDCM0441  CL50 HEK-293T C356(1.03)  LDD1645  [7]
 LDCM0443  CL52 HEK-293T C356(0.82); C889(1.14)  LDD1646  [7]
 LDCM0445  CL54 HEK-293T C889(1.04)  LDD1648  [7]
 LDCM0446  CL55 HEK-293T C356(1.13)  LDD1649  [7]
 LDCM0447  CL56 HEK-293T C356(1.52)  LDD1650  [7]
 LDCM0448  CL57 HEK-293T C356(1.08)  LDD1651  [7]
 LDCM0449  CL58 HEK-293T C356(1.05)  LDD1652  [7]
 LDCM0450  CL59 HEK-293T C356(1.35); C959(0.80)  LDD1653  [7]
 LDCM0451  CL6 HEK-293T C889(1.13)  LDD1654  [7]
 LDCM0452  CL60 HEK-293T C356(1.18)  LDD1655  [7]
 LDCM0454  CL62 HEK-293T C356(0.90)  LDD1657  [7]
 LDCM0456  CL64 HEK-293T C356(0.97); C889(1.12)  LDD1659  [7]
 LDCM0458  CL66 HEK-293T C889(1.17)  LDD1661  [7]
 LDCM0459  CL67 HEK-293T C356(1.20)  LDD1662  [7]
 LDCM0460  CL68 HEK-293T C356(1.36)  LDD1663  [7]
 LDCM0461  CL69 HEK-293T C356(0.97)  LDD1664  [7]
 LDCM0462  CL7 HEK-293T C356(1.04)  LDD1665  [7]
 LDCM0463  CL70 HEK-293T C356(1.05)  LDD1666  [7]
 LDCM0464  CL71 HEK-293T C356(1.08); C959(0.74)  LDD1667  [7]
 LDCM0465  CL72 HEK-293T C356(1.08)  LDD1668  [7]
 LDCM0467  CL74 HEK-293T C356(0.99)  LDD1670  [7]
 LDCM0469  CL76 HEK-293T C356(0.89); C889(0.98)  LDD1672  [7]
 LDCM0471  CL78 HEK-293T C889(1.11)  LDD1674  [7]
 LDCM0472  CL79 HEK-293T C356(1.10)  LDD1675  [7]
 LDCM0473  CL8 HEK-293T C356(1.33)  LDD1676  [7]
 LDCM0474  CL80 HEK-293T C356(1.18)  LDD1677  [7]
 LDCM0475  CL81 HEK-293T C356(0.93)  LDD1678  [7]
 LDCM0476  CL82 HEK-293T C356(0.92)  LDD1679  [7]
 LDCM0477  CL83 HEK-293T C356(1.23); C959(0.63)  LDD1680  [7]
 LDCM0478  CL84 HEK-293T C356(1.05)  LDD1681  [7]
 LDCM0480  CL86 HEK-293T C356(1.42)  LDD1683  [7]
 LDCM0482  CL88 HEK-293T C356(0.85); C889(1.08)  LDD1685  [7]
 LDCM0484  CL9 HEK-293T C356(0.89)  LDD1687  [7]
 LDCM0485  CL90 HEK-293T C889(1.10)  LDD1688  [7]
 LDCM0486  CL91 HEK-293T C356(1.13)  LDD1689  [7]
 LDCM0487  CL92 HEK-293T C356(1.09)  LDD1690  [7]
 LDCM0488  CL93 HEK-293T C356(0.88)  LDD1691  [7]
 LDCM0489  CL94 HEK-293T C356(0.94)  LDD1692  [7]
 LDCM0490  CL95 HEK-293T C356(1.56); C959(0.63)  LDD1693  [7]
 LDCM0491  CL96 HEK-293T C356(1.12)  LDD1694  [7]
 LDCM0493  CL98 HEK-293T C356(0.71)  LDD1696  [7]
 LDCM0427  Fragment51 HEK-293T C356(1.23)  LDD1631  [7]
 LDCM0022  KB02 HCT 116 C559(1.66); C573(1.66)  LDD0080  [4]
 LDCM0023  KB03 HCT 116 C559(1.07); C573(1.07)  LDD0081  [4]
 LDCM0024  KB05 HCT 116 C559(1.37); C573(1.37)  LDD0082  [4]

References

1 Oxidant-Induced Bioconjugation for Protein Labeling in Live Cells. ACS Chem Biol. 2023 Jan 20;18(1):112-122. doi: 10.1021/acschembio.2c00740. Epub 2022 Dec 21.
2 An Azo Coupling-Based Chemoproteomic Approach to Systematically Profile the Tyrosine Reactivity in the Human Proteome. Anal Chem. 2021 Jul 27;93(29):10334-10342. doi: 10.1021/acs.analchem.1c01935. Epub 2021 Jul 12.
3 Quantitative and Site-Specific Chemoproteomic Profiling of Targets of Acrolein. Chem Res Toxicol. 2019 Mar 18;32(3):467-473. doi: 10.1021/acs.chemrestox.8b00343. Epub 2019 Jan 15.
4 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
5 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
6 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
7 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402