General Information of Target

Target ID LDTP01405
Target Name Decapping and exoribonuclease protein (DXO)
Gene Name DXO
Gene ID 1797
Synonyms
DOM3L; DOM3Z; NG6; Decapping and exoribonuclease protein; DXO; EC 3.6.1.-; 5'-3' exoribonuclease DXO; EC 3.1.13.-; Dom-3 homolog Z; NAD-capped RNA hydrolase DXO; DeNADding enzyme DXO; EC 3.6.1.-
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MDPRGTKRGAEKTEVAEPRNKLPRPAPSLPTDPALYSGPFPFYRRPSELGCFSLDAQRQY
HGDARALRYYSPPPTNGPGPNFDLRDGYPDRYQPRDEEVQERLDHLLCWLLEHRGRLEGG
PGWLAEAIVTWRGHLTKLLTTPYERQEGWQLAASRFQGTLYLSEVETPNARAQRLARPPL
LRELMYMGYKFEQYMCADKPGSSPDPSGEVNTNVAFCSVLRSRLGSHPLLFSGEVDCTDP
QAPSTQPPTCYVELKTSKEMHSPGQWRSFYRHKLLKWWAQSFLPGVPNVVAGFRNPDGFV
SSLKTFPTMKMFEYVRNDRDGWNPSVCMNFCAAFLSFAQSTVVQDDPRLVHLFSWEPGGP
VTVSVHQDAPYAFLPIWYVEAMTQDLPSPPKTPSPK
Target Bioclass
Enzyme
Family
DXO/Dom3Z family
Subcellular location
Nucleus
Function
Decapping enzyme for NAD-capped RNAs: specifically hydrolyzes the nicotinamide adenine dinucleotide (NAD) cap from a subset of RNAs by removing the entire NAD moiety from the 5'-end of an NAD-capped RNA. The NAD-cap is present at the 5'-end of some RNAs and snoRNAs. In contrast to the canonical 5'-end N7 methylguanosine (m7G) cap, the NAD cap promotes mRNA decay. Preferentially acts on NAD-capped transcripts in response to environmental stress. Also acts as a non-canonical decapping enzyme that removes the entire cap structure of m7G capped or incompletely capped RNAs and mediates their subsequent degradation. Specifically degrades pre-mRNAs with a defective 5'-end m7G cap and is part of a pre-mRNA capping quality control. Has decapping activity toward incomplete 5'-end m7G cap mRNAs such as unmethylated 5'-end-capped RNA (cap0), while it has no activity toward 2'-O-ribose methylated m7G cap (cap1). In contrast to canonical decapping enzymes DCP2 and NUDT16, which cleave the cap within the triphosphate linkage, the decapping activity releases the entire cap structure GpppN and a 5'-end monophosphate RNA. Also has 5'-3' exoribonuclease activities: The 5'-end monophosphate RNA is then degraded by the 5'-3' exoribonuclease activity, enabling this enzyme to decap and degrade incompletely capped mRNAs. Also possesses RNA 5'-pyrophosphohydrolase activity by hydrolyzing the 5'-end triphosphate to release pyrophosphates. Exhibits decapping activity towards FAD-capped RNAs. Exhibits decapping activity towards dpCoA-capped RNAs in vitro.
Uniprot ID
O77932
Ensemble ID
ENST00000337523.10
HGNC ID
HGNC:2992

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 4 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
Probe 1
 Probe Info 
Y314(107.65)  LDD3495  [1]
DBIA
 Probe Info 
C108(0.92)  LDD3351  [2]
IA-alkyne
 Probe Info 
N.A.  LDD0036  [3]
NAIA_5
 Probe Info 
C108(0.00); C51(0.00)  LDD2223  [4]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0214  AC1 HEK-293T C108(1.04)  LDD1507  [5]
 LDCM0215  AC10 PaTu 8988t C108(0.78)  LDD1094  [6]
 LDCM0226  AC11 PaTu 8988t C108(0.79)  LDD1105  [6]
 LDCM0237  AC12 PaTu 8988t C108(0.84)  LDD1116  [6]
 LDCM0259  AC14 PaTu 8988t C108(1.03)  LDD1138  [6]
 LDCM0270  AC15 PaTu 8988t C108(0.83)  LDD1149  [6]
 LDCM0276  AC17 HEK-293T C108(0.91)  LDD1515  [5]
 LDCM0277  AC18 HEK-293T C108(0.97)  LDD1516  [5]
 LDCM0278  AC19 HEK-293T C108(0.55)  LDD1517  [5]
 LDCM0279  AC2 HEK-293T C108(0.89)  LDD1518  [5]
 LDCM0280  AC20 HEK-293T C108(1.14)  LDD1519  [5]
 LDCM0281  AC21 HEK-293T C108(0.91)  LDD1520  [5]
 LDCM0282  AC22 HEK-293T C108(1.11)  LDD1521  [5]
 LDCM0283  AC23 HEK-293T C108(1.00)  LDD1522  [5]
 LDCM0284  AC24 HEK-293T C108(0.86)  LDD1523  [5]
 LDCM0285  AC25 HEK-293T C108(0.93)  LDD1524  [5]
 LDCM0286  AC26 HEK-293T C108(1.15)  LDD1525  [5]
 LDCM0287  AC27 HEK-293T C108(1.01)  LDD1526  [5]
 LDCM0288  AC28 HEK-293T C108(1.11)  LDD1527  [5]
 LDCM0289  AC29 HEK-293T C108(0.83)  LDD1528  [5]
 LDCM0290  AC3 HEK-293T C108(1.04)  LDD1529  [5]
 LDCM0291  AC30 HEK-293T C108(0.86)  LDD1530  [5]
 LDCM0292  AC31 HEK-293T C108(1.13)  LDD1531  [5]
 LDCM0293  AC32 HEK-293T C108(0.99)  LDD1532  [5]
 LDCM0294  AC33 HEK-293T C108(0.94)  LDD1533  [5]
 LDCM0295  AC34 HEK-293T C108(0.90)  LDD1534  [5]
 LDCM0296  AC35 HEK-293T C108(1.17)  LDD1535  [5]
 LDCM0297  AC36 HEK-293T C108(1.00)  LDD1536  [5]
 LDCM0298  AC37 HEK-293T C108(0.91)  LDD1537  [5]
 LDCM0299  AC38 HEK-293T C108(1.06)  LDD1538  [5]
 LDCM0300  AC39 HEK-293T C108(1.21)  LDD1539  [5]
 LDCM0301  AC4 HEK-293T C108(1.05)  LDD1540  [5]
 LDCM0302  AC40 HEK-293T C108(1.11)  LDD1541  [5]
 LDCM0303  AC41 HEK-293T C108(1.02)  LDD1542  [5]
 LDCM0304  AC42 HEK-293T C108(0.90)  LDD1543  [5]
 LDCM0305  AC43 HEK-293T C108(1.00)  LDD1544  [5]
 LDCM0306  AC44 HEK-293T C108(0.97)  LDD1545  [5]
 LDCM0307  AC45 HEK-293T C108(0.92)  LDD1546  [5]
 LDCM0308  AC46 HEK-293T C108(0.83)  LDD1547  [5]
 LDCM0309  AC47 HEK-293T C108(0.90)  LDD1548  [5]
 LDCM0310  AC48 HEK-293T C108(1.03)  LDD1549  [5]
 LDCM0311  AC49 HEK-293T C108(0.93)  LDD1550  [5]
 LDCM0312  AC5 HEK-293T C108(1.18)  LDD1551  [5]
 LDCM0313  AC50 HEK-293T C108(0.92)  LDD1552  [5]
 LDCM0314  AC51 HEK-293T C108(0.92)  LDD1553  [5]
 LDCM0315  AC52 HEK-293T C108(1.02)  LDD1554  [5]
 LDCM0316  AC53 HEK-293T C108(0.90)  LDD1555  [5]
 LDCM0317  AC54 HEK-293T C108(0.87)  LDD1556  [5]
 LDCM0318  AC55 HEK-293T C108(1.16)  LDD1557  [5]
 LDCM0319  AC56 HEK-293T C108(0.99)  LDD1558  [5]
 LDCM0320  AC57 HEK-293T C108(1.01)  LDD1559  [5]
 LDCM0321  AC58 HEK-293T C108(0.97)  LDD1560  [5]
 LDCM0322  AC59 HEK-293T C108(0.90)  LDD1561  [5]
 LDCM0323  AC6 PaTu 8988t C108(0.92)  LDD1202  [6]
 LDCM0324  AC60 HEK-293T C108(0.93)  LDD1563  [5]
 LDCM0325  AC61 HEK-293T C108(0.93)  LDD1564  [5]
 LDCM0326  AC62 HEK-293T C108(0.85)  LDD1565  [5]
 LDCM0327  AC63 HEK-293T C108(1.04)  LDD1566  [5]
 LDCM0328  AC64 HEK-293T C108(0.97)  LDD1567  [5]
 LDCM0334  AC7 PaTu 8988t C108(0.77)  LDD1213  [6]
 LDCM0345  AC8 PaTu 8988t C108(0.90)  LDD1224  [6]
 LDCM0248  AKOS034007472 PaTu 8988t C108(0.99)  LDD1127  [6]
 LDCM0356  AKOS034007680 PaTu 8988t C108(0.45)  LDD1235  [6]
 LDCM0275  AKOS034007705 PaTu 8988t C108(0.70)  LDD1154  [6]
 LDCM0367  CL1 HEK-293T C108(0.97)  LDD1571  [5]
 LDCM0368  CL10 HEK-293T C108(0.76)  LDD1572  [5]
 LDCM0369  CL100 HEK-293T C108(1.06)  LDD1573  [5]
 LDCM0370  CL101 PaTu 8988t C108(0.94)  LDD1249  [6]
 LDCM0371  CL102 PaTu 8988t C108(0.86)  LDD1250  [6]
 LDCM0372  CL103 PaTu 8988t C108(0.80)  LDD1251  [6]
 LDCM0373  CL104 PaTu 8988t C108(0.87)  LDD1252  [6]
 LDCM0374  CL105 HEK-293T C108(0.93)  LDD1578  [5]
 LDCM0375  CL106 HEK-293T C108(0.92)  LDD1579  [5]
 LDCM0376  CL107 HEK-293T C108(1.16)  LDD1580  [5]
 LDCM0377  CL108 HEK-293T C108(0.83)  LDD1581  [5]
 LDCM0378  CL109 HEK-293T C108(0.92)  LDD1582  [5]
 LDCM0379  CL11 HEK-293T C108(1.15)  LDD1583  [5]
 LDCM0380  CL110 HEK-293T C108(0.94)  LDD1584  [5]
 LDCM0381  CL111 HEK-293T C108(0.86)  LDD1585  [5]
 LDCM0382  CL112 HEK-293T C108(0.99)  LDD1586  [5]
 LDCM0383  CL113 HEK-293T C108(0.95)  LDD1587  [5]
 LDCM0384  CL114 HEK-293T C108(1.05)  LDD1588  [5]
 LDCM0385  CL115 HEK-293T C108(0.99)  LDD1589  [5]
 LDCM0386  CL116 HEK-293T C108(1.30)  LDD1590  [5]
 LDCM0387  CL117 HEK-293T C108(0.92)  LDD1591  [5]
 LDCM0388  CL118 HEK-293T C108(1.04)  LDD1592  [5]
 LDCM0389  CL119 HEK-293T C108(0.78)  LDD1593  [5]
 LDCM0390  CL12 HEK-293T C108(0.70)  LDD1594  [5]
 LDCM0391  CL120 HEK-293T C108(1.15)  LDD1595  [5]
 LDCM0392  CL121 HEK-293T C108(1.03)  LDD1596  [5]
 LDCM0393  CL122 HEK-293T C108(1.28)  LDD1597  [5]
 LDCM0394  CL123 HEK-293T C108(0.83)  LDD1598  [5]
 LDCM0395  CL124 HEK-293T C108(1.14)  LDD1599  [5]
 LDCM0396  CL125 HEK-293T C108(0.87)  LDD1600  [5]
 LDCM0397  CL126 HEK-293T C108(0.94)  LDD1601  [5]
 LDCM0398  CL127 HEK-293T C108(0.97)  LDD1602  [5]
 LDCM0399  CL128 HEK-293T C108(1.38)  LDD1603  [5]
 LDCM0400  CL13 HEK-293T C108(0.84)  LDD1604  [5]
 LDCM0401  CL14 HEK-293T C108(1.00)  LDD1605  [5]
 LDCM0402  CL15 HEK-293T C108(0.74)  LDD1606  [5]
 LDCM0403  CL16 PaTu 8988t C108(1.16)  LDD1282  [6]
 LDCM0404  CL17 PaTu 8988t C108(0.95)  LDD1283  [6]
 LDCM0405  CL18 PaTu 8988t C108(0.81)  LDD1284  [6]
 LDCM0406  CL19 PaTu 8988t C108(0.86)  LDD1285  [6]
 LDCM0407  CL2 HEK-293T C108(0.84)  LDD1611  [5]
 LDCM0408  CL20 PaTu 8988t C108(0.59)  LDD1287  [6]
 LDCM0409  CL21 PaTu 8988t C108(0.60)  LDD1288  [6]
 LDCM0410  CL22 PaTu 8988t C108(0.73)  LDD1289  [6]
 LDCM0411  CL23 PaTu 8988t C108(0.98)  LDD1290  [6]
 LDCM0412  CL24 PaTu 8988t C108(1.04)  LDD1291  [6]
 LDCM0413  CL25 PaTu 8988t C108(1.24)  LDD1292  [6]
 LDCM0414  CL26 PaTu 8988t C108(0.74)  LDD1293  [6]
 LDCM0415  CL27 PaTu 8988t C108(1.19)  LDD1294  [6]
 LDCM0416  CL28 PaTu 8988t C108(0.68)  LDD1295  [6]
 LDCM0417  CL29 PaTu 8988t C108(0.71)  LDD1296  [6]
 LDCM0418  CL3 HEK-293T C108(0.87)  LDD1622  [5]
 LDCM0419  CL30 PaTu 8988t C108(1.41)  LDD1298  [6]
 LDCM0420  CL31 PaTu 8988t C108(1.14)  LDD1299  [6]
 LDCM0421  CL32 PaTu 8988t C108(1.18)  LDD1300  [6]
 LDCM0422  CL33 PaTu 8988t C108(0.88)  LDD1301  [6]
 LDCM0423  CL34 PaTu 8988t C108(1.07)  LDD1302  [6]
 LDCM0424  CL35 PaTu 8988t C108(0.87)  LDD1303  [6]
 LDCM0425  CL36 PaTu 8988t C108(1.20)  LDD1304  [6]
 LDCM0426  CL37 PaTu 8988t C108(0.78)  LDD1305  [6]
 LDCM0428  CL39 PaTu 8988t C108(1.15)  LDD1307  [6]
 LDCM0429  CL4 HEK-293T C108(0.83)  LDD1633  [5]
 LDCM0430  CL40 PaTu 8988t C108(1.08)  LDD1309  [6]
 LDCM0431  CL41 PaTu 8988t C108(0.75)  LDD1310  [6]
 LDCM0432  CL42 PaTu 8988t C108(0.46)  LDD1311  [6]
 LDCM0433  CL43 PaTu 8988t C108(0.32)  LDD1312  [6]
 LDCM0434  CL44 PaTu 8988t C108(0.78)  LDD1313  [6]
 LDCM0435  CL45 PaTu 8988t C108(1.06)  LDD1314  [6]
 LDCM0436  CL46 HEK-293T C108(0.79)  LDD1640  [5]
 LDCM0437  CL47 HEK-293T C108(0.98)  LDD1641  [5]
 LDCM0438  CL48 HEK-293T C108(0.75)  LDD1642  [5]
 LDCM0439  CL49 HEK-293T C108(1.00)  LDD1643  [5]
 LDCM0440  CL5 HEK-293T C108(0.93)  LDD1644  [5]
 LDCM0441  CL50 HEK-293T C108(0.96)  LDD1645  [5]
 LDCM0443  CL52 HEK-293T C108(0.90)  LDD1646  [5]
 LDCM0444  CL53 HEK-293T C108(0.68)  LDD1647  [5]
 LDCM0445  CL54 HEK-293T C108(0.28)  LDD1648  [5]
 LDCM0446  CL55 HEK-293T C108(0.98)  LDD1649  [5]
 LDCM0447  CL56 HEK-293T C108(1.04)  LDD1650  [5]
 LDCM0448  CL57 HEK-293T C108(0.77)  LDD1651  [5]
 LDCM0449  CL58 HEK-293T C108(1.00)  LDD1652  [5]
 LDCM0450  CL59 HEK-293T C108(1.04)  LDD1653  [5]
 LDCM0451  CL6 HEK-293T C108(0.83)  LDD1654  [5]
 LDCM0452  CL60 HEK-293T C108(1.01)  LDD1655  [5]
 LDCM0453  CL61 HEK-293T C108(1.01)  LDD1656  [5]
 LDCM0454  CL62 HEK-293T C108(1.00)  LDD1657  [5]
 LDCM0455  CL63 HEK-293T C108(1.00)  LDD1658  [5]
 LDCM0456  CL64 HEK-293T C108(1.15)  LDD1659  [5]
 LDCM0457  CL65 HEK-293T C108(0.82)  LDD1660  [5]
 LDCM0458  CL66 HEK-293T C108(0.85)  LDD1661  [5]
 LDCM0459  CL67 HEK-293T C108(0.91)  LDD1662  [5]
 LDCM0460  CL68 HEK-293T C108(0.88)  LDD1663  [5]
 LDCM0461  CL69 HEK-293T C108(1.08)  LDD1664  [5]
 LDCM0462  CL7 HEK-293T C108(0.97)  LDD1665  [5]
 LDCM0463  CL70 HEK-293T C108(0.90)  LDD1666  [5]
 LDCM0464  CL71 HEK-293T C108(1.00)  LDD1667  [5]
 LDCM0465  CL72 HEK-293T C108(0.95)  LDD1668  [5]
 LDCM0466  CL73 HEK-293T C108(1.00)  LDD1669  [5]
 LDCM0467  CL74 HEK-293T C108(1.03)  LDD1670  [5]
 LDCM0469  CL76 PaTu 8988t C108(0.85)  LDD1348  [6]
 LDCM0470  CL77 PaTu 8988t C108(0.81)  LDD1349  [6]
 LDCM0471  CL78 PaTu 8988t C108(0.94)  LDD1350  [6]
 LDCM0472  CL79 PaTu 8988t C108(0.88)  LDD1351  [6]
 LDCM0473  CL8 HEK-293T C108(0.93)  LDD1676  [5]
 LDCM0474  CL80 PaTu 8988t C108(1.19)  LDD1353  [6]
 LDCM0475  CL81 PaTu 8988t C108(0.98)  LDD1354  [6]
 LDCM0476  CL82 PaTu 8988t C108(1.02)  LDD1355  [6]
 LDCM0477  CL83 PaTu 8988t C108(0.83)  LDD1356  [6]
 LDCM0478  CL84 PaTu 8988t C108(0.68)  LDD1357  [6]
 LDCM0479  CL85 PaTu 8988t C108(0.94)  LDD1358  [6]
 LDCM0480  CL86 PaTu 8988t C108(1.23)  LDD1359  [6]
 LDCM0481  CL87 PaTu 8988t C108(0.66)  LDD1360  [6]
 LDCM0482  CL88 PaTu 8988t C108(0.89)  LDD1361  [6]
 LDCM0483  CL89 PaTu 8988t C108(0.67)  LDD1362  [6]
 LDCM0484  CL9 HEK-293T C108(0.90)  LDD1687  [5]
 LDCM0485  CL90 PaTu 8988t C108(0.89)  LDD1364  [6]
 LDCM0486  CL91 HEK-293T C108(0.93)  LDD1689  [5]
 LDCM0487  CL92 HEK-293T C108(1.02)  LDD1690  [5]
 LDCM0488  CL93 HEK-293T C108(0.91)  LDD1691  [5]
 LDCM0489  CL94 HEK-293T C108(0.81)  LDD1692  [5]
 LDCM0490  CL95 HEK-293T C108(0.73)  LDD1693  [5]
 LDCM0491  CL96 HEK-293T C108(0.76)  LDD1694  [5]
 LDCM0492  CL97 HEK-293T C108(0.86)  LDD1695  [5]
 LDCM0493  CL98 HEK-293T C108(0.93)  LDD1696  [5]
 LDCM0494  CL99 HEK-293T C108(0.97)  LDD1697  [5]
 LDCM0495  E2913 HEK-293T C108(1.07)  LDD1698  [5]
 LDCM0468  Fragment33 HEK-293T C108(0.92)  LDD1671  [5]
 LDCM0427  Fragment51 PaTu 8988t C108(1.05)  LDD1306  [6]
 LDCM0022  KB02 HEK-293T C108(0.98)  LDD1492  [5]
 LDCM0023  KB03 HEK-293T C108(1.06)  LDD1497  [5]
 LDCM0024  KB05 NCI-H196 C108(0.92)  LDD3351  [2]

References

1 An Azo Coupling-Based Chemoproteomic Approach to Systematically Profile the Tyrosine Reactivity in the Human Proteome. Anal Chem. 2021 Jul 27;93(29):10334-10342. doi: 10.1021/acs.analchem.1c01935. Epub 2021 Jul 12.
2 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
3 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
4 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
5 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
6 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.