Details of the Target

General Information of Target

Target ID LDTP00766
Target Name Eukaryotic translation initiation factor 4 gamma 3 (EIF4G3)
Gene Name EIF4G3
Gene ID 8672
Synonyms
Eukaryotic translation initiation factor 4 gamma 3; eIF-4-gamma 3; eIF-4G 3; eIF4G 3; eIF-4-gamma II; eIF4GII
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MNSQPQTRSPFFQRPQIQPPRATIPNSSPSIRPGAQTPTAVYQANQHIMMVNHLPMPYPV
PQGPQYCIPQYRHSGPPYVGPPQQYPVQPPGPGPFYPGPGPGDFPNAYGTPFYPSQPVYQ
SAPIIVPTQQQPPPAKREKKTIRIRDPNQGGKDITEEIMSGGGSRNPTPPIGRPTSTPTP
PQQLPSQVPEHSPVVYGTVESAHLAASTPVTAASDQKQEEKPKPDPVLKSPSPVLRLVLS
GEKKEQEGQTSETTAIVSIAELPLPPSPTTVSSVARSTIAAPTSSALSSQPIFTTAIDDR
CELSSPREDTIPIPSLTSCTETSDPLPTNENDDDICKKPCSVAPNDIPLVSSTNLINEIN
GVSEKLSATESIVEIVKQEVLPLTLELEILENPPEEMKLECIPAPITPSTVPSFPPTPPT
PPASPPHTPVIVPAAATTVSSPSAAITVQRVLEEDESIRTCLSEDAKEIQNKIEVEADGQ
TEEILDSQNLNSRRSPVPAQIAITVPKTWKKPKDRTRTTEEMLEAELELKAEEELSIDKV
LESEQDKMSQGFHPERDPSDLKKVKAVEENGEEAEPVRNGAESVSEGEGIDANSGSTDSS
GDGVTFPFKPESWKPTDTEGKKQYDREFLLDFQFMPACIQKPEGLPPISDVVLDKINQPK
LPMRTLDPRILPRGPDFTPAFADFGRQTPGGRGVPLLNVGSRRSQPGQRREPRKIITVSV
KEDVHLKKAENAWKPSQKRDSQADDPENIKTQELFRKVRSILNKLTPQMFNQLMKQVSGL
TVDTEERLKGVIDLVFEKAIDEPSFSVAYANMCRCLVTLKVPMADKPGNTVNFRKLLLNR
CQKEFEKDKADDDVFEKKQKELEAASAPEERTRLHDELEEAKDKARRRSIGNIKFIGELF
KLKMLTEAIMHDCVVKLLKNHDEESLECLCRLLTTIGKDLDFEKAKPRMDQYFNQMEKIV
KERKTSSRIRFMLQDVIDLRLCNWVSRRADQGPKTIEQIHKEAKIEEQEEQRKVQQLMTK
EKRRPGVQRVDEGGWNTVQGAKNSRVLDPSKFLKITKPTIDEKIQLVPKAQLGSWGKGSS
GGAKASETDALRSSASSLNRFSALQPPAPSGSTPSTPVEFDSRRTLTSRGSMGREKNDKP
LPSATARPNTFMRGGSSKDLLDNQSQEEQRREMLETVKQLTGGVDVERNSTEAERNKTRE
SAKPEISAMSAHDKAALSEEELERKSKSIIDEFLHINDFKEAMQCVEELNAQGLLHVFVR
VGVESTLERSQITRDHMGQLLYQLVQSEKLSKQDFFKGFSETLELADDMAIDIPHIWLYL
AELVTPMLKEGGISMRELTIEFSKPLLPVGRAGVLLSEILHLLCKQMSHKKVGALWREAD
LSWKDFLPEGEDVHNFLLEQKLDFIESDSPCSSEALSKKELSAEELYKRLEKLIIEDKAN
DEQIFDWVEANLDEIQMSSPTFLRALMTAVCKAAIIADSSTFRVDTAVIKQRVPILLKYL
DSDTEKELQALYALQASIVKLDQPANLLRMFFDCLYDEEVISEDAFYKWESSKDPAEQNG
KGVALKSVTAFFTWLREAEEESEDN
Target Bioclass
Other
Family
Eukaryotic initiation factor 4G family
Function
Component of the protein complex eIF4F, which is involved in the recognition of the mRNA cap, ATP-dependent unwinding of 5'-terminal secondary structure and recruitment of mRNA to the ribosome. Functional homolog of EIF4G1.
Uniprot ID
O43432
Ensemble ID
ENST00000264211.13
HGNC ID
HGNC:3298

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 20 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
m-APA
 Probe Info  		10.61  LDD0402  [1]
STPyne
 Probe Info  		K1051(10.00)  LDD0277  [2]
THZ1-DTB
 Probe Info  		C928(1.15)  LDD0460  [3]
BTD
 Probe Info  		C1471(0.52)  LDD2109  [4]
DA-P3
 Probe Info  		4.44  LDD0181  [5]
AHL-Pu-1
 Probe Info  		C1411(2.37)  LDD0169  [6]
DBIA
 Probe Info  		C982(14.07)  LDD0204  [7]
Acrolein
 Probe Info  		N.A.  LDD0222  [8]
AMP probe
 Probe Info  		N.A.  LDD0200  [9]
ATP probe
 Probe Info  		K919(0.00); K903(0.00)  LDD0199  [9]
4-Iodoacetamidophenylacetylene
 Probe Info  		N.A.  LDD0038  [10]
IA-alkyne
 Probe Info  		N.A.  LDD0032  [11]
Lodoacetamide azide
 Probe Info  		C1364(0.00); C982(0.00); C461(0.00)  LDD0037  [10]
NAIA_4
 Probe Info  		N.A.  LDD2226  [12]
TFBX
 Probe Info  		C928(0.00); C930(0.00); C913(0.00)  LDD0027  [13]
IPM
 Probe Info  		C461(0.00); C841(0.00)  LDD0147  [13]
Methacrolein
 Probe Info  		C1411(0.00); C461(0.00)  LDD0218  [8]
W1
 Probe Info  		C928(0.00); C930(0.00); C1411(0.00)  LDD0236  [14]
AOyne
 Probe Info  		6.10  LDD0443  [15]
NAIA_5
 Probe Info  		C1364(0.00); C841(0.00); C913(0.00)  LDD2223  [12]
PAL-AfBPP Probe
Click To Hide/Show 1 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
STS-2
 Probe Info  		N.A.  LDD0139  [16]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0537  2-Cyano-N,N-dimethylacetamide MDA-MB-231 C461(0.84)  LDD2130  [4]
 LDCM0026  4SU-RNA+native RNA HEK-293T C1411(2.37)  LDD0169  [6]
 LDCM0156  Aniline NCI-H1299 11.53  LDD0403  [1]
 LDCM0102  BDHI 8 Jurkat C982(14.07)  LDD0204  [7]
 LDCM0087  Capsaicin HEK-293T 4.37  LDD0185  [5]
 LDCM0108  Chloroacetamide HeLa N.A.  LDD0222  [8]
 LDCM0632  CL-Sc Hep-G2 C1411(20.00); C461(0.95)  LDD2227  [12]
 LDCM0213  Electrophilic fragment 2 MDA-MB-231 C982(3.64)  LDD1702  [4]
 LDCM0625  F8 Ramos C813(1.18)  LDD2187  [17]
 LDCM0572  Fragment10 Ramos C813(0.63)  LDD2189  [17]
 LDCM0573  Fragment11 Ramos C813(7.74); C1364(20.00)  LDD2190  [17]
 LDCM0574  Fragment12 Ramos C813(0.72)  LDD2191  [17]
 LDCM0575  Fragment13 Ramos C813(0.95)  LDD2192  [17]
 LDCM0576  Fragment14 Ramos C813(0.80); C1364(1.63)  LDD2193  [17]
 LDCM0579  Fragment20 Ramos C813(0.54)  LDD2194  [17]
 LDCM0580  Fragment21 Ramos C813(1.10)  LDD2195  [17]
 LDCM0582  Fragment23 Ramos C813(0.72)  LDD2196  [17]
 LDCM0578  Fragment27 Ramos C813(1.09)  LDD2197  [17]
 LDCM0586  Fragment28 Ramos C813(1.21); C1364(1.22)  LDD2198  [17]
 LDCM0588  Fragment30 Ramos C813(0.81)  LDD2199  [17]
 LDCM0589  Fragment31 Ramos C813(1.08)  LDD2200  [17]
 LDCM0590  Fragment32 Ramos C813(0.61)  LDD2201  [17]
 LDCM0468  Fragment33 Ramos C813(0.82)  LDD2202  [17]
 LDCM0596  Fragment38 Ramos C813(1.07)  LDD2203  [17]
 LDCM0566  Fragment4 Ramos C813(0.99); C1364(1.20)  LDD2184  [17]
 LDCM0610  Fragment52 Ramos C813(0.87)  LDD2204  [17]
 LDCM0614  Fragment56 Ramos C813(1.29)  LDD2205  [17]
 LDCM0569  Fragment7 Ramos C813(0.95); C1364(1.27)  LDD2186  [17]
 LDCM0571  Fragment9 Ramos C813(0.53)  LDD2188  [17]
 LDCM0022  KB02 Ramos C813(0.87); C1364(0.66)  LDD2182  [17]
 LDCM0023  KB03 Jurkat C913(8.36); C982(57.64)  LDD0209  [7]
 LDCM0024  KB05 G361 C312(1.24); C988(3.31); C472(1.55)  LDD3311  [18]
 LDCM0109  NEM HeLa N.A.  LDD0223  [8]
 LDCM0516  Nucleophilic fragment 21a MDA-MB-231 C1471(0.52)  LDD2109  [4]
 LDCM0527  Nucleophilic fragment 26b MDA-MB-231 C1471(1.39)  LDD2120  [4]
 LDCM0542  Nucleophilic fragment 37 MDA-MB-231 C1471(1.00)  LDD2135  [4]
 LDCM0559  Nucleophilic fragment 9b MDA-MB-231 C913(3.11)  LDD2153  [4]
 LDCM0029  Quercetin HEK-293T 4.44  LDD0181  [5]
 LDCM0021  THZ1 HeLa S3 C928(1.15)  LDD0460  [3]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Other
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Eukaryotic translation initiation factor 4E (EIF4E) Eukaryotic initiation factor 4E family P06730

References

1 Quantitative and Site-Specific Chemoproteomic Profiling of Targets of Acrolein. Chem Res Toxicol. 2019 Mar 18;32(3):467-473. doi: 10.1021/acs.chemrestox.8b00343. Epub 2019 Jan 15.
2 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
3 A Chemoproteomic Strategy for Direct and Proteome-Wide Covalent Inhibitor Target-Site Identification. J Am Chem Soc. 2019 Jan 9;141(1):191-203. doi: 10.1021/jacs.8b07911. Epub 2018 Dec 20.
4 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
5 A chemical probe unravels the reactive proteome of health-associated catechols. Chem Sci. 2023 Jul 22;14(32):8635-8643. doi: 10.1039/d3sc00888f. eCollection 2023 Aug 16.
Mass spectrometry data entry: PXD043348
6 Chemoproteomic capture of RNA binding activity in living cells. Nat Commun. 2023 Oct 7;14(1):6282. doi: 10.1038/s41467-023-41844-z.
Mass spectrometry data entry: PXD044625
7 Covalent Inhibition by a Natural Product-Inspired Latent Electrophile. J Am Chem Soc. 2023 May 24;145(20):11097-11109. doi: 10.1021/jacs.3c00598. Epub 2023 May 15.
8 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
9 Targeted Proteomic Approaches for Proteome-Wide Characterizations of the AMP-Binding Capacities of Kinases. J Proteome Res. 2022 Aug 5;21(8):2063-2070. doi: 10.1021/acs.jproteome.2c00225. Epub 2022 Jul 12.
10 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
11 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
12 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
13 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
14 Oxidant-Induced Bioconjugation for Protein Labeling in Live Cells. ACS Chem Biol. 2023 Jan 20;18(1):112-122. doi: 10.1021/acschembio.2c00740. Epub 2022 Dec 21.
15 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.
16 Design and synthesis of minimalist terminal alkyne-containing diazirine photo-crosslinkers and their incorporation into kinase inhibitors for cell- and tissue-based proteome profiling. Angew Chem Int Ed Engl. 2013 Aug 12;52(33):8551-6. doi: 10.1002/anie.201300683. Epub 2013 Jun 10.
17 Site-specific quantitative cysteine profiling with data-independent acquisition-based mass spectrometry. Methods Enzymol. 2023;679:295-322. doi: 10.1016/bs.mie.2022.07.037. Epub 2022 Sep 7.
Mass spectrometry data entry: PXD027578
18 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840