Details of the Target

General Information of Target

Target ID LDTP00404
Target Name Lysosomal cobalamin transporter ABCD4 (ABCD4)
Gene Name ABCD4
Gene ID 5826
Synonyms
PXMP1L; Lysosomal cobalamin transporter ABCD4; EC 7.6.2.8; ATP-binding cassette sub-family D member 4; PMP70-related protein; P70R; Peroxisomal membrane protein 1-like; PXMP1-L; Peroxisomal membrane protein 69; PMP69
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MAVAGPAPGAGARPRLDLQFLQRFLQILKVLFPSWSSQNALMFLTLLCLTLLEQFVIYQV
GLIPSQYYGVLGNKDLEGFKTLTFLAVMLIVLNSTLKSFDQFTCNLLYVSWRKDLTEHLH
RLYFRGRAYYTLNVLRDDIDNPDQRISQDVERFCRQLSSMASKLIISPFTLVYYTYQCFQ
STGWLGPVSIFGYFILGTVVNKTLMGPIVMKLVHQEKLEGDFRFKHMQIRVNAEPAAFYR
AGHVEHMRTDRRLQRLLQTQRELMSKELWLYIGINTFDYLGSILSYVVIAIPIFSGVYGD
LSPAELSTLVSKNAFVCIYLISCFTQLIDLSTTLSDVAGYTHRIGQLRETLLDMSLKSQD
CEILGESEWGLDTPPGWPAAEPADTAFLLERVSISAPSSDKPLIKDLSLKISEGQSLLIT
GNTGTGKTSLLRVLGGLWTSTRGSVQMLTDFGPHGVLFLPQKPFFTDGTLREQVIYPLKE
VYPDSGSADDERILRFLELAGLSNLVARTEGLDQQVDWNWYDVLSPGEMQRLSFARLFYL
QPKYAVLDEATSALTEEVESELYRIGQQLGMTFISVGHRQSLEKFHSLVLKLCGGGRWEL
MRIKVE
Target Bioclass
Enzyme
Family
ABC transporter superfamily, ABCD family, Peroxisomal fatty acyl CoA transporter (TC 3.A.1.203) subfamily
Subcellular location
Endoplasmic reticulum membrane
Function
Lysosomal membrane protein that transports cobalamin (Vitamin B12) from the lysosomal lumen to the cytosol in an ATP-dependent manner. Targeted by LMBRD1 lysosomal chaperone from the endoplasmic reticulum to the lysosomal membrane. Then forms a complex with lysosomal chaperone LMBRD1 and cytosolic MMACHC to transport cobalamin across the lysosomal membrane.
Uniprot ID
O14678
Ensemble ID
ENST00000356924.9
HGNC ID
HGNC:68

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 2 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C361(0.95)  LDD1492  [1]
ATP probe
 Probe Info  		N.A.  LDD0199  [2]
PAL-AfBPP Probe
Click To Hide/Show 2 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C004
 Probe Info  		5.70  LDD1714  [3]
C070
 Probe Info  		8.51  LDD1766  [3]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0214  AC1 HEK-293T C361(1.14)  LDD1507  [1]
 LDCM0215  AC10 HEK-293T C361(1.00)  LDD1508  [1]
 LDCM0270  AC15 HEK-293T C361(1.01)  LDD1513  [1]
 LDCM0276  AC17 HEK-293T C361(1.04)  LDD1515  [1]
 LDCM0277  AC18 HEK-293T C361(0.99)  LDD1516  [1]
 LDCM0279  AC2 HEK-293T C361(0.93)  LDD1518  [1]
 LDCM0281  AC21 HEK-293T C361(1.35)  LDD1520  [1]
 LDCM0283  AC23 HEK-293T C361(1.14)  LDD1522  [1]
 LDCM0284  AC24 HEK-293T C361(0.94)  LDD1523  [1]
 LDCM0285  AC25 HEK-293T C361(1.04)  LDD1524  [1]
 LDCM0286  AC26 HEK-293T C361(0.96)  LDD1525  [1]
 LDCM0289  AC29 HEK-293T C361(0.90)  LDD1528  [1]
 LDCM0292  AC31 HEK-293T C361(0.94)  LDD1531  [1]
 LDCM0293  AC32 HEK-293T C361(0.93)  LDD1532  [1]
 LDCM0294  AC33 HEK-293T C361(1.03)  LDD1533  [1]
 LDCM0295  AC34 HEK-293T C361(0.79)  LDD1534  [1]
 LDCM0298  AC37 HEK-293T C361(1.09)  LDD1537  [1]
 LDCM0300  AC39 HEK-293T C361(1.17)  LDD1539  [1]
 LDCM0302  AC40 HEK-293T C361(0.86)  LDD1541  [1]
 LDCM0303  AC41 HEK-293T C361(0.99)  LDD1542  [1]
 LDCM0304  AC42 HEK-293T C361(1.08)  LDD1543  [1]
 LDCM0307  AC45 HEK-293T C361(1.19)  LDD1546  [1]
 LDCM0309  AC47 HEK-293T C361(0.98)  LDD1548  [1]
 LDCM0310  AC48 HEK-293T C361(1.04)  LDD1549  [1]
 LDCM0311  AC49 HEK-293T C361(1.02)  LDD1550  [1]
 LDCM0312  AC5 HEK-293T C361(1.12)  LDD1551  [1]
 LDCM0313  AC50 HEK-293T C361(0.85)  LDD1552  [1]
 LDCM0316  AC53 HEK-293T C361(1.08)  LDD1555  [1]
 LDCM0318  AC55 HEK-293T C361(1.06)  LDD1557  [1]
 LDCM0319  AC56 HEK-293T C361(1.11)  LDD1558  [1]
 LDCM0320  AC57 HEK-293T C361(1.20)  LDD1559  [1]
 LDCM0321  AC58 HEK-293T C361(0.88)  LDD1560  [1]
 LDCM0325  AC61 HEK-293T C361(1.04)  LDD1564  [1]
 LDCM0327  AC63 HEK-293T C361(1.14)  LDD1566  [1]
 LDCM0328  AC64 HEK-293T C361(1.41)  LDD1567  [1]
 LDCM0334  AC7 HEK-293T C361(1.01)  LDD1568  [1]
 LDCM0345  AC8 HEK-293T C361(0.87)  LDD1569  [1]
 LDCM0248  AKOS034007472 HEK-293T C361(1.64)  LDD1511  [1]
 LDCM0356  AKOS034007680 HEK-293T C361(1.02)  LDD1570  [1]
 LDCM0275  AKOS034007705 HEK-293T C361(1.06)  LDD1514  [1]
 LDCM0379  CL11 HEK-293T C361(1.14)  LDD1583  [1]
 LDCM0390  CL12 HEK-293T C361(1.76)  LDD1594  [1]
 LDCM0404  CL17 HEK-293T C361(1.16)  LDD1608  [1]
 LDCM0405  CL18 HEK-293T C361(0.88)  LDD1609  [1]
 LDCM0409  CL21 HEK-293T C361(1.17)  LDD1613  [1]
 LDCM0411  CL23 HEK-293T C361(1.12)  LDD1615  [1]
 LDCM0412  CL24 HEK-293T C361(1.21)  LDD1616  [1]
 LDCM0417  CL29 HEK-293T C361(1.05)  LDD1621  [1]
 LDCM0419  CL30 HEK-293T C361(0.84)  LDD1623  [1]
 LDCM0422  CL33 HEK-293T C361(1.38)  LDD1626  [1]
 LDCM0424  CL35 HEK-293T C361(0.99)  LDD1628  [1]
 LDCM0425  CL36 HEK-293T C361(0.86)  LDD1629  [1]
 LDCM0431  CL41 HEK-293T C361(1.11)  LDD1635  [1]
 LDCM0432  CL42 HEK-293T C361(1.15)  LDD1636  [1]
 LDCM0435  CL45 HEK-293T C361(1.30)  LDD1639  [1]
 LDCM0437  CL47 HEK-293T C361(0.89)  LDD1641  [1]
 LDCM0438  CL48 HEK-293T C361(0.91)  LDD1642  [1]
 LDCM0440  CL5 HEK-293T C361(1.20)  LDD1644  [1]
 LDCM0444  CL53 HEK-293T C361(1.00)  LDD1647  [1]
 LDCM0445  CL54 HEK-293T C361(0.89)  LDD1648  [1]
 LDCM0448  CL57 HEK-293T C361(1.04)  LDD1651  [1]
 LDCM0450  CL59 HEK-293T C361(0.99)  LDD1653  [1]
 LDCM0451  CL6 HEK-293T C361(0.93)  LDD1654  [1]
 LDCM0452  CL60 HEK-293T C361(1.10)  LDD1655  [1]
 LDCM0457  CL65 HEK-293T C361(1.04)  LDD1660  [1]
 LDCM0458  CL66 HEK-293T C361(0.90)  LDD1661  [1]
 LDCM0461  CL69 HEK-293T C361(1.31)  LDD1664  [1]
 LDCM0464  CL71 HEK-293T C361(1.32)  LDD1667  [1]
 LDCM0465  CL72 HEK-293T C361(1.39)  LDD1668  [1]
 LDCM0470  CL77 HEK-293T C361(0.96)  LDD1673  [1]
 LDCM0471  CL78 HEK-293T C361(0.82)  LDD1674  [1]
 LDCM0475  CL81 HEK-293T C361(1.37)  LDD1678  [1]
 LDCM0477  CL83 HEK-293T C361(0.98)  LDD1680  [1]
 LDCM0478  CL84 HEK-293T C361(1.65)  LDD1681  [1]
 LDCM0483  CL89 HEK-293T C361(1.05)  LDD1686  [1]
 LDCM0484  CL9 HEK-293T C361(0.97)  LDD1687  [1]
 LDCM0485  CL90 HEK-293T C361(0.91)  LDD1688  [1]
 LDCM0488  CL93 HEK-293T C361(1.37)  LDD1691  [1]
 LDCM0490  CL95 HEK-293T C361(1.08)  LDD1693  [1]
 LDCM0491  CL96 HEK-293T C361(1.26)  LDD1694  [1]
 LDCM0022  KB02 HEK-293T C361(0.95)  LDD1492  [1]
 LDCM0023  KB03 HEK-293T C361(1.01)  LDD1497  [1]
 LDCM0024  KB05 HEK-293T C361(0.88)  LDD1502  [1]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Lysosomal cobalamin transporter ABCD4 (ABCD4) ABCD family O14678
Transporter and channel
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Lysosomal cobalamin transport escort protein LMBD1 (LMBRD1) LIMR family Q9NUN5

References

1 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
2 Targeted Proteomic Approaches for Proteome-Wide Characterizations of the AMP-Binding Capacities of Kinases. J Proteome Res. 2022 Aug 5;21(8):2063-2070. doi: 10.1021/acs.jproteome.2c00225. Epub 2022 Jul 12.
3 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587