Details of the Target

General Information of Target

Target ID LDTP00311
Target Name Membrane-associated phosphatidylinositol transfer protein 1 (PITPNM1)
Gene Name PITPNM1
Gene ID 9600
Synonyms
DRES9; NIR2; PITPNM; Membrane-associated phosphatidylinositol transfer protein 1; Drosophila retinal degeneration B homolog; Phosphatidylinositol transfer protein, membrane-associated 1; PITPnm 1; Pyk2 N-terminal domain-interacting receptor 2; NIR-2
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MLIKEYHILLPMSLDEYQVAQLYMIQKKSREESSGEGSGVEILANRPYTDGPGGSGQYTH
KVYHVGSHIPGWFRALLPKAALQVEEESWNAYPYTRTRYTCPFVEKFSIEIETYYLPDGG
QQPNVFNLSGAERRQRILDTIDIVRDAVAPGEYKAEEDPRLYHSVKTGRGPLSDDWARTA
AQTGPLMCAYKLCKVEFRYWGMQAKIEQFIHDVGLRRVMLRAHRQAWCWQDEWTELSMAD
IRALEEETARMLAQRMAKCNTGSEGSEAQPPGKPSTEARSAASNTGTPDGPEAPPGPDAS
PDASFGKQWSSSSRSSYSSQHGGAVSPQSLSEWRMQNIARDSENSSEEEFFDAHEGFSDS
EEVFPKEMTKWNSNDFIDAFASPVEAEGTPEPGAEAAKGIEDGAQAPRDSEGLDGAGELG
AEACAVHALFLILHSGNILDSGPGDANSKQADVQTLSSAFEAVTRIHFPEALGHVALRLV
PCPPICAAAYALVSNLSPYSHDGDSLSRSQDHIPLAALPLLATSSSRYQGAVATVIARTN
QAYSAFLRSPEGAGFCGQVALIGDGVGGILGFDALCHSANAGTGSRGSSRRGSMNNELLS
PEFGPVRDPLADGVEGLGRGSPEPSALPPQRIPSDMASPEPEGSQNSLQAAPATTSSWEP
RRASTAFCPPAASSEAPDGPSSTARLDFKVSGFFLFGSPLGLVLALRKTVMPALEAAQMR
PACEQIYNLFHAADPCASRLEPLLAPKFQAIAPLTVPRYQKFPLGDGSSLLLADTLQTHS
SLFLEELEMLVPSTPTSTSGAFWKGSELATDPPAQPAAPSTTSEVVKILERWWGTKRIDY
SLYCPEALTAFPTVTLPHLFHASYWESADVVAFILRQVIEKERPQLAECEEPSIYSPAFP
REKWQRKRTQVKIRNVTSNHRASDTVVCEGRPQVLSGRFMYGPLDVVTLTGEKVDVYIMT
QPLSGKWIHFGTEVTNSSGRLTFPVPPERALGIGVYPVRMVVRGDHTYAECCLTVVARGT
EAVVFSIDGSFTASVSIMGSDPKVRAGAVDVVRHWQDSGYLIVYVTGRPDMQKHRVVAWL
SQHNFPHGVVSFCDGLTHDPLRQKAMFLQSLVQEVELNIVAGYGSPKDVAVYAALGLSPS
QTYIVGRAVRKLQAQCQFLSDGYVAHLGQLEAGSHSHASSGPPRAALGKSSYGVAAPVDF
LRKQSQLLRSRGPSQAEREGPGTPPTTLARGKARSISLKLDSEE
Target Bioclass
Transporter and channel
Family
PtdIns transfer protein family, PI transfer class IIA subfamily
Subcellular location
Cytoplasm
Function
Catalyzes the transfer of phosphatidylinositol (PI) between membranes. Binds PI, phosphatidylcholine (PC) and phosphatidic acid (PA) with the binding affinity order of PI > PA > PC. Regulates RHOA activity, and plays a role in cytoskeleton remodeling. Necessary for normal completion of cytokinesis. Plays a role in maintaining normal diacylglycerol levels in the Golgi apparatus. Necessary for maintaining the normal structure of the endoplasmic reticulum and the Golgi apparatus. Required for protein export from the endoplasmic reticulum and the Golgi. Binds calcium ions.
Uniprot ID
O00562
Ensemble ID
ENST00000356404.8
HGNC ID
HGNC:9003
ChEMBL ID
CHEMBL1764937

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
CAL78 SNV: p.G582C DBIA    Probe Info 
CCK81 Deletion: p.P295QfsTer74 DBIA    Probe Info 
HCT15 SNV: p.E277D .
HT115 SNV: p.R221W .
JURKAT SNV: p.A626S .
NCIH1299 SNV: p.G356C .
NCIH1793 SNV: p.G643D .
SUPT1 SNV: p.R707H DBIA    Probe Info 

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 8 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C259(2.52)  LDD3312  [1]
IA-alkyne
 Probe Info  		N.A.  LDD0162  [2]
JW-RF-010
 Probe Info  		N.A.  LDD0026  [3]
NAIA_4
 Probe Info  		N.A.  LDD2226  [4]
NAIA_5
 Probe Info  		N.A.  LDD2224  [4]
WYneN
 Probe Info  		N.A.  LDD0021  [5]
IPM
 Probe Info  		N.A.  LDD0147  [3]
TFBX
 Probe Info  		N.A.  LDD0148  [3]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0215  AC10 PaTu 8988t C228(0.76)  LDD1094  [6]
 LDCM0226  AC11 PaTu 8988t C228(0.99)  LDD1105  [6]
 LDCM0237  AC12 PaTu 8988t C228(0.88)  LDD1116  [6]
 LDCM0259  AC14 PaTu 8988t C228(1.09)  LDD1138  [6]
 LDCM0270  AC15 PaTu 8988t C228(0.87)  LDD1149  [6]
 LDCM0277  AC18 HEK-293T C259(1.24); C668(0.98)  LDD1516  [7]
 LDCM0279  AC2 HEK-293T C259(1.01); C668(0.86)  LDD1518  [7]
 LDCM0280  AC20 HEK-293T C259(1.19)  LDD1519  [7]
 LDCM0281  AC21 HEK-293T C259(1.11)  LDD1520  [7]
 LDCM0284  AC24 HEK-293T C259(0.78); C668(1.00)  LDD1523  [7]
 LDCM0286  AC26 HEK-293T C259(1.17); C668(0.96)  LDD1525  [7]
 LDCM0288  AC28 HEK-293T C259(1.13)  LDD1527  [7]
 LDCM0289  AC29 HEK-293T C259(1.00)  LDD1528  [7]
 LDCM0293  AC32 HEK-293T C259(1.27); C668(1.01)  LDD1532  [7]
 LDCM0295  AC34 HEK-293T C259(1.16); C668(0.88)  LDD1534  [7]
 LDCM0297  AC36 HEK-293T C259(1.30)  LDD1536  [7]
 LDCM0298  AC37 HEK-293T C259(1.15)  LDD1537  [7]
 LDCM0301  AC4 HEK-293T C259(1.35)  LDD1540  [7]
 LDCM0302  AC40 HEK-293T C259(0.98); C668(1.25)  LDD1541  [7]
 LDCM0304  AC42 HEK-293T C259(1.24); C668(1.15)  LDD1543  [7]
 LDCM0306  AC44 HEK-293T C259(1.20)  LDD1545  [7]
 LDCM0307  AC45 HEK-293T C259(0.97)  LDD1546  [7]
 LDCM0310  AC48 HEK-293T C259(1.21); C668(1.35)  LDD1549  [7]
 LDCM0312  AC5 HEK-293T C259(1.15)  LDD1551  [7]
 LDCM0313  AC50 HEK-293T C259(1.05); C668(1.34)  LDD1552  [7]
 LDCM0315  AC52 HEK-293T C259(1.38)  LDD1554  [7]
 LDCM0316  AC53 HEK-293T C259(0.96)  LDD1555  [7]
 LDCM0319  AC56 HEK-293T C259(1.32); C668(0.99)  LDD1558  [7]
 LDCM0321  AC58 HEK-293T C259(1.00); C668(1.18)  LDD1560  [7]
 LDCM0323  AC6 PaTu 8988t C228(0.83)  LDD1202  [6]
 LDCM0324  AC60 HEK-293T C259(1.11)  LDD1563  [7]
 LDCM0325  AC61 HEK-293T C259(1.24)  LDD1564  [7]
 LDCM0328  AC64 HEK-293T C259(1.37); C668(0.98)  LDD1567  [7]
 LDCM0334  AC7 PaTu 8988t C228(0.89)  LDD1213  [6]
 LDCM0345  AC8 PaTu 8988t C228(0.86)  LDD1224  [6]
 LDCM0248  AKOS034007472 PaTu 8988t C228(1.13)  LDD1127  [6]
 LDCM0356  AKOS034007680 PaTu 8988t C228(0.93)  LDD1235  [6]
 LDCM0275  AKOS034007705 PaTu 8988t C228(0.82)  LDD1154  [6]
 LDCM0632  CL-Sc Hep-G2 C259(0.55)  LDD2227  [4]
 LDCM0369  CL100 HEK-293T C259(0.90); C668(0.75)  LDD1573  [7]
 LDCM0370  CL101 PaTu 8988t C228(0.68)  LDD1249  [6]
 LDCM0371  CL102 PaTu 8988t C228(0.87)  LDD1250  [6]
 LDCM0372  CL103 PaTu 8988t C228(1.02)  LDD1251  [6]
 LDCM0373  CL104 PaTu 8988t C228(0.81)  LDD1252  [6]
 LDCM0375  CL106 HEK-293T C259(1.04)  LDD1579  [7]
 LDCM0376  CL107 HEK-293T C259(1.00)  LDD1580  [7]
 LDCM0377  CL108 HEK-293T C259(1.19); C668(0.89)  LDD1581  [7]
 LDCM0380  CL110 HEK-293T C259(1.02)  LDD1584  [7]
 LDCM0381  CL111 HEK-293T C259(0.91)  LDD1585  [7]
 LDCM0382  CL112 HEK-293T C259(1.15); C668(0.70)  LDD1586  [7]
 LDCM0384  CL114 HEK-293T C259(0.94)  LDD1588  [7]
 LDCM0385  CL115 HEK-293T C259(1.04)  LDD1589  [7]
 LDCM0386  CL116 HEK-293T C259(1.19); C668(0.83)  LDD1590  [7]
 LDCM0388  CL118 HEK-293T C259(1.28)  LDD1592  [7]
 LDCM0389  CL119 HEK-293T C259(1.02)  LDD1593  [7]
 LDCM0390  CL12 HEK-293T C259(1.07); C668(1.01)  LDD1594  [7]
 LDCM0391  CL120 HEK-293T C259(1.11); C668(1.86)  LDD1595  [7]
 LDCM0393  CL122 HEK-293T C259(1.20)  LDD1597  [7]
 LDCM0394  CL123 HEK-293T C259(0.88)  LDD1598  [7]
 LDCM0395  CL124 HEK-293T C259(0.93); C668(0.78)  LDD1599  [7]
 LDCM0397  CL126 HEK-293T C259(0.98)  LDD1601  [7]
 LDCM0398  CL127 HEK-293T C259(0.94)  LDD1602  [7]
 LDCM0399  CL128 HEK-293T C259(1.06); C668(0.98)  LDD1603  [7]
 LDCM0401  CL14 HEK-293T C259(1.10)  LDD1605  [7]
 LDCM0402  CL15 HEK-293T C259(0.97)  LDD1606  [7]
 LDCM0403  CL16 HEK-293T C259(1.11); C668(0.93)  LDD1607  [7]
 LDCM0405  CL18 HEK-293T C259(1.24); C668(1.06)  LDD1609  [7]
 LDCM0407  CL2 HEK-293T C259(1.09)  LDD1611  [7]
 LDCM0408  CL20 HEK-293T C259(1.04)  LDD1612  [7]
 LDCM0409  CL21 HEK-293T C259(1.02)  LDD1613  [7]
 LDCM0412  CL24 HEK-293T C259(0.97); C668(1.08)  LDD1616  [7]
 LDCM0414  CL26 HEK-293T C259(0.98)  LDD1618  [7]
 LDCM0415  CL27 HEK-293T C259(1.07)  LDD1619  [7]
 LDCM0416  CL28 HEK-293T C259(1.01); C668(0.72)  LDD1620  [7]
 LDCM0418  CL3 HEK-293T C259(0.96)  LDD1622  [7]
 LDCM0419  CL30 HEK-293T C259(1.07); C668(1.24)  LDD1623  [7]
 LDCM0420  CL31 PaTu 8988t C228(0.64)  LDD1299  [6]
 LDCM0421  CL32 PaTu 8988t C228(0.55)  LDD1300  [6]
 LDCM0422  CL33 PaTu 8988t C228(0.66)  LDD1301  [6]
 LDCM0423  CL34 PaTu 8988t C228(0.66)  LDD1302  [6]
 LDCM0424  CL35 PaTu 8988t C228(0.70)  LDD1303  [6]
 LDCM0425  CL36 PaTu 8988t C228(0.18)  LDD1304  [6]
 LDCM0426  CL37 PaTu 8988t C228(0.59)  LDD1305  [6]
 LDCM0428  CL39 PaTu 8988t C228(0.57)  LDD1307  [6]
 LDCM0429  CL4 HEK-293T C259(0.98); C668(0.75)  LDD1633  [7]
 LDCM0430  CL40 PaTu 8988t C228(0.56)  LDD1309  [6]
 LDCM0431  CL41 PaTu 8988t C228(0.74)  LDD1310  [6]
 LDCM0432  CL42 PaTu 8988t C228(0.49)  LDD1311  [6]
 LDCM0433  CL43 PaTu 8988t C228(0.58)  LDD1312  [6]
 LDCM0434  CL44 PaTu 8988t C228(0.50)  LDD1313  [6]
 LDCM0435  CL45 PaTu 8988t C228(0.44)  LDD1314  [6]
 LDCM0436  CL46 PaTu 8988t C228(0.78)  LDD1315  [6]
 LDCM0437  CL47 PaTu 8988t C228(0.85)  LDD1316  [6]
 LDCM0438  CL48 PaTu 8988t C228(0.87)  LDD1317  [6]
 LDCM0439  CL49 PaTu 8988t C228(0.78)  LDD1318  [6]
 LDCM0441  CL50 PaTu 8988t C228(0.64)  LDD1320  [6]
 LDCM0442  CL51 PaTu 8988t C228(0.69)  LDD1321  [6]
 LDCM0443  CL52 PaTu 8988t C228(0.68)  LDD1322  [6]
 LDCM0444  CL53 PaTu 8988t C228(0.53)  LDD1323  [6]
 LDCM0445  CL54 PaTu 8988t C228(0.75)  LDD1324  [6]
 LDCM0446  CL55 PaTu 8988t C228(0.73)  LDD1325  [6]
 LDCM0447  CL56 PaTu 8988t C228(0.42)  LDD1326  [6]
 LDCM0448  CL57 PaTu 8988t C228(0.93)  LDD1327  [6]
 LDCM0449  CL58 PaTu 8988t C228(0.71)  LDD1328  [6]
 LDCM0450  CL59 PaTu 8988t C228(0.74)  LDD1329  [6]
 LDCM0451  CL6 HEK-293T C259(0.99); C668(1.33)  LDD1654  [7]
 LDCM0452  CL60 PaTu 8988t C228(0.73)  LDD1331  [6]
 LDCM0453  CL61 PaTu 8988t C228(0.91)  LDD1332  [6]
 LDCM0454  CL62 PaTu 8988t C228(1.01)  LDD1333  [6]
 LDCM0455  CL63 PaTu 8988t C228(1.01)  LDD1334  [6]
 LDCM0456  CL64 PaTu 8988t C228(0.92)  LDD1335  [6]
 LDCM0457  CL65 PaTu 8988t C228(0.92)  LDD1336  [6]
 LDCM0458  CL66 PaTu 8988t C228(1.13)  LDD1337  [6]
 LDCM0459  CL67 PaTu 8988t C228(1.05)  LDD1338  [6]
 LDCM0460  CL68 PaTu 8988t C228(0.93)  LDD1339  [6]
 LDCM0461  CL69 PaTu 8988t C228(1.06)  LDD1340  [6]
 LDCM0463  CL70 PaTu 8988t C228(0.97)  LDD1342  [6]
 LDCM0464  CL71 PaTu 8988t C228(1.08)  LDD1343  [6]
 LDCM0465  CL72 PaTu 8988t C228(0.97)  LDD1344  [6]
 LDCM0466  CL73 PaTu 8988t C228(1.02)  LDD1345  [6]
 LDCM0467  CL74 PaTu 8988t C228(0.88)  LDD1346  [6]
 LDCM0469  CL76 PaTu 8988t C228(1.06)  LDD1348  [6]
 LDCM0470  CL77 PaTu 8988t C228(1.12)  LDD1349  [6]
 LDCM0471  CL78 PaTu 8988t C228(1.15)  LDD1350  [6]
 LDCM0472  CL79 PaTu 8988t C228(1.28)  LDD1351  [6]
 LDCM0473  CL8 HEK-293T C259(0.86)  LDD1676  [7]
 LDCM0474  CL80 PaTu 8988t C228(1.06)  LDD1353  [6]
 LDCM0475  CL81 PaTu 8988t C228(0.99)  LDD1354  [6]
 LDCM0476  CL82 PaTu 8988t C228(1.15)  LDD1355  [6]
 LDCM0477  CL83 PaTu 8988t C228(0.87)  LDD1356  [6]
 LDCM0478  CL84 PaTu 8988t C228(1.27)  LDD1357  [6]
 LDCM0479  CL85 PaTu 8988t C228(1.01)  LDD1358  [6]
 LDCM0480  CL86 PaTu 8988t C228(0.86)  LDD1359  [6]
 LDCM0481  CL87 PaTu 8988t C228(0.94)  LDD1360  [6]
 LDCM0482  CL88 PaTu 8988t C228(0.99)  LDD1361  [6]
 LDCM0483  CL89 PaTu 8988t C228(0.99)  LDD1362  [6]
 LDCM0484  CL9 HEK-293T C259(0.96)  LDD1687  [7]
 LDCM0485  CL90 PaTu 8988t C228(0.99)  LDD1364  [6]
 LDCM0487  CL92 HEK-293T C259(1.02)  LDD1690  [7]
 LDCM0488  CL93 HEK-293T C259(0.92)  LDD1691  [7]
 LDCM0491  CL96 HEK-293T C259(0.91); C668(1.19)  LDD1694  [7]
 LDCM0493  CL98 HEK-293T C259(0.89)  LDD1696  [7]
 LDCM0494  CL99 HEK-293T C259(1.02)  LDD1697  [7]
 LDCM0495  E2913 HEK-293T C259(1.07)  LDD1698  [7]
 LDCM0468  Fragment33 PaTu 8988t C228(0.84)  LDD1347  [6]
 LDCM0427  Fragment51 PaTu 8988t C228(0.58)  LDD1306  [6]
 LDCM0022  KB02 HEK-293T C259(0.98)  LDD1492  [7]
 LDCM0023  KB03 HEK-293T C259(1.11)  LDD1497  [7]
 LDCM0024  KB05 HMCB C259(2.52)  LDD3312  [1]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Transcription factor
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Alpha-globin transcription factor CP2 (TFCP2) Grh/CP2 family Q12800

References

1 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
2 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
3 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
4 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
5 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
6 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
7 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402