Details of the Target

General Information of Target

Target ID LDTP00042
Target Name Probable ribonuclease ZC3H12D (ZC3H12D)
Gene Name ZC3H12D
Gene ID 340152
Synonyms
C6orf95; MCPIP4; TFL; Probable ribonuclease ZC3H12D; EC 3.1.-.-; MCP-induced protein 4; Transformed follicular lymphoma; Zinc finger CCCH domain-containing protein 12D; p34
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MEHPSKMEFFQKLGYDREDVLRVLGKLGEGALVNDVLQELIRTGSRPGALEHPAAPRLVP
RGSCGVPDSAQRGPGTALEEDFRTLASSLRPIVIDGSNVAMSHGNKETFSCRGIKLAVDW
FRDRGHTYIKVFVPSWRKDPPRADTPIREQHVLAELERQAVLVYTPSRKVHGKRLVCYDD
RYIVKVAYEQDGVIVSNDNYRDLQSENPEWKWFIEQRLLMFSFVNDRFMPPDDPLGRHGP
SLSNFLSRKPKPPEPSWQHCPYGKKCTYGIKCKFYHPERPHHAQLAVADELRAKTGARPG
AGAEEQRPPRAPGGSAGARAAPREPFAHSLPPARGSPDLAALRGSFSRLAFSDDLGPLGP
PLPVPACSLTPRLGGPDWVSAGGRVPGPLSLPSPESQFSPGDLPPPPGLQLQPRGEHRPR
DLHGDLLSPRRPPDDPWARPPRSDRFPGRSVWAEPAWGDGATGGLSVYATEDDEGDARAR
ARIALYSVFPRDQVDRVMAAFPELSDLARLILLVQRCQSAGAPLGKP
Target Bioclass
Enzyme
Family
ZC3H12 family
Subcellular location
Cytoplasm; Cytoplasm, P-body
Function
May regulate cell growth likely by suppressing RB1 phosphorylation. May function as RNase and regulate the levels of target RNA species (Potential). In association with ZC3H12A enhances the degradation of interleukin IL-6 mRNA level in activated macrophages. Serve as a tumor suppressor in certain leukemia cells. Overexpression inhibits the G1 to S phase progression through suppression of RB1 phosphorylation.
Uniprot ID
A2A288
Ensemble ID
ENST00000409806.8
HGNC ID
HGNC:21175

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
CAL27 SNV: p.D68A .
HCT15 SNV: p.P261H .
PC9 SNV: p.K526N .
SKMEL3 SNV: p.Q515Ter .
SNU1 SNV: p.V451I .
TE11 Insertion: p.L358_P360dup .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 2 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C312(1.35)  LDD3325  [1]
IA-alkyne
 Probe Info  		12.59  LDD0431  [2]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0625  F8 Ramos C64(1.28); C517(1.18); C177(1.85)  LDD2187  [3]
 LDCM0572  Fragment10 Ramos C64(2.30); C517(1.10); C177(0.99)  LDD2189  [3]
 LDCM0573  Fragment11 Ramos C64(1.87); C111(6.54)  LDD2190  [3]
 LDCM0574  Fragment12 Ramos C64(1.19); C517(0.89); C177(1.16)  LDD2191  [3]
 LDCM0575  Fragment13 Ramos C64(0.96); C517(0.50); C177(1.48); C367(0.78)  LDD2192  [3]
 LDCM0576  Fragment14 Ramos C64(1.04); C517(1.09); C177(0.62); C367(2.41)  LDD2193  [3]
 LDCM0579  Fragment20 Ramos C64(1.68); C177(0.55)  LDD2194  [3]
 LDCM0580  Fragment21 Ramos C64(0.97); C517(0.41); C177(2.02); C367(0.90)  LDD2195  [3]
 LDCM0582  Fragment23 Ramos C64(0.92); C517(0.51); C177(0.62); C367(0.67)  LDD2196  [3]
 LDCM0578  Fragment27 Ramos C64(0.83); C517(0.42); C177(1.08); C367(0.91)  LDD2197  [3]
 LDCM0586  Fragment28 Ramos C64(0.81); C517(0.43); C177(1.08); C367(0.79)  LDD2198  [3]
 LDCM0587  Fragment29 Ramos C367(1.33)  LDD1476  [4]
 LDCM0588  Fragment30 Ramos C64(1.26); C517(0.50); C177(1.07); C367(1.27)  LDD2199  [3]
 LDCM0589  Fragment31 Ramos C367(1.88)  LDD1478  [4]
 LDCM0590  Fragment32 Ramos C64(1.93); C517(1.00)  LDD2201  [3]
 LDCM0468  Fragment33 Ramos C64(1.25); C517(0.65); C177(2.58); C367(1.48)  LDD2202  [3]
 LDCM0596  Fragment38 Ramos C64(0.98); C517(0.56); C177(0.81); C367(0.95)  LDD2203  [3]
 LDCM0566  Fragment4 Ramos C64(1.39); C517(1.60); C177(1.02); C367(1.04)  LDD2184  [3]
 LDCM0610  Fragment52 Ramos C64(1.16); C517(0.70); C177(1.25)  LDD2204  [3]
 LDCM0614  Fragment56 Ramos C64(1.30); C517(0.52); C177(1.00)  LDD2205  [3]
 LDCM0569  Fragment7 Ramos C64(1.45); C517(1.87); C177(0.97)  LDD2186  [3]
 LDCM0571  Fragment9 Ramos C367(5.20)  LDD1464  [4]
 LDCM0022  KB02 Ramos 12.59  LDD0431  [2]
 LDCM0023  KB03 Ramos C64(1.07); C517(1.67); C177(0.97); C111(1.56)  LDD2183  [3]
 LDCM0024  KB05 UACC257 C312(1.35)  LDD3325  [1]

References

1 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
2 2-Sulfonylpyridines as Tunable, Cysteine-Reactive Electrophiles. J Am Chem Soc. 2020 May 13;142(19):8972-8979. doi: 10.1021/jacs.0c02721. Epub 2020 Apr 29.
3 Site-specific quantitative cysteine profiling with data-independent acquisition-based mass spectrometry. Methods Enzymol. 2023;679:295-322. doi: 10.1016/bs.mie.2022.07.037. Epub 2022 Sep 7.
Mass spectrometry data entry: PXD027578
4 Proteome-wide covalent ligand discovery in native biological systems. Nature. 2016 Jun 23;534(7608):570-4. doi: 10.1038/nature18002. Epub 2016 Jun 15.