Details of the Target

General Information of Target

Target ID LDTP14895
Target Name Eukaryotic translation initiation factor 2 subunit 3B (EIF2S3B)
Gene Name EIF2S3B
Synonyms
Eukaryotic translation initiation factor 2 subunit 3B; EC 3.6.5.3; Eukaryotic translation initiation factor 2 subunit gamma A; eIF-2-gamma A; eIF-2gA
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MSPQGPAVLSLGSLCLDTNQAPNWTGLQTLLQQLPPQDIDERYCLALGEEERAELQLFCA
RRKQEALGQGVARLVLPKLEGHTCEKCRELLKPGEYGVFAARAGEQRCWHQPCFACQACG
QALINLIYFYHDGQLYCGRHHAELLRPRCPACDQLIFSWRCTEAEGQRWHENHFCCQDCA
GPLGGGRYALPGGSPCCPSCFENRYSDAGSSWAGALEGQAFLGETGLDRTEGRDQTSVNS
ATLSRTLLAAAGGSSLQTQRGLPGSSPQQENRPGDKAEAPKGQEQCRLETIRDPKDTPFS
TCSSSSDSEPEGFFLGERLPQSWKTPGSLQAEDSNASKTHCTMC
Target Bioclass
Enzyme
Family
TRAFAC class translation factor GTPase superfamily, Classic translation factor GTPase family, EIF2G subfamily
Function
Member of the eIF2 complex that functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form the 43S pre-initiation complex (43S PIC). Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF2 and release of an eIF2-GDP binary complex. In order for eIF2 to recycle and catalyze another round of initiation, the GDP bound to eIF2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.
Uniprot ID
Q2VIR3
Ensemble ID
ENST00000322446.3
HGNC ID
HGNC:43863

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
22RV1 SNV: p.L158M DBIA    Probe Info 
COLO678 Insertion: p.E5GfsTer11 DBIA    Probe Info 
COLO679 SNV: p.G409R DBIA    Probe Info 
CORL88 SNV: p.Q329Ter DBIA    Probe Info 
HEC1 SNV: p.S225L DBIA    Probe Info 
HUH1 SNV: p.L371S DBIA    Probe Info 
NCIH2291 Insertion: p.V319CfsTer8 DBIA    Probe Info 
PEO1 Deletion: p.P345_D351del DBIA    Probe Info 
SKOV3 SNV: p.R460S DBIA    Probe Info 

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 10 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
TH211
 Probe Info  		Y330(5.65)  LDD0257  [1]
AZ-9
 Probe Info  		E5(10.00)  LDD2209  [2]
DBIA
 Probe Info  		C348(2.82); C236(1.30)  LDD3310  [3]
BTD
 Probe Info  		C348(1.66)  LDD1700  [4]
AHL-Pu-1
 Probe Info  		C269(2.03)  LDD0169  [5]
IPM
 Probe Info  		C96(0.52)  LDD1701  [4]
Acrolein
 Probe Info  		C269(0.00); C434(0.00); H325(0.00); H184(0.00)  LDD0217  [6]
Cinnamaldehyde
 Probe Info  		C348(0.00); C434(0.00)  LDD0220  [6]
Crotonaldehyde
 Probe Info  		C434(0.00); C348(0.00)  LDD0219  [6]
Methacrolein
 Probe Info  		C434(0.00); C269(0.00); C348(0.00); C101(0.00)  LDD0218  [6]
PAL-AfBPP Probe
Click To Hide/Show 1 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
STS-1
 Probe Info  		N.A.  LDD0137  [7]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0548  1-(4-(Benzo[d][1,3]dioxol-5-ylmethyl)piperazin-1-yl)-2-nitroethan-1-one MDA-MB-231 C348(0.39)  LDD2142  [4]
 LDCM0519  1-(6-methoxy-3,4-dihydroquinolin-1(2H)-yl)-2-nitroethan-1-one MDA-MB-231 C348(0.67); C269(0.65)  LDD2112  [4]
 LDCM0524  2-Cyano-N-(2-morpholin-4-yl-ethyl)-acetamide MDA-MB-231 C434(0.87); C269(0.95); C101(1.09); C96(0.64)  LDD2117  [4]
 LDCM0558  2-Cyano-N-phenylacetamide MDA-MB-231 C434(1.41); C348(1.00)  LDD2152  [4]
 LDCM0538  4-(Cyanoacetyl)morpholine MDA-MB-231 C348(1.58)  LDD2131  [4]
 LDCM0026  4SU-RNA+native RNA HEK-293T C269(2.03)  LDD0169  [5]
 LDCM0520  AKOS000195272 MDA-MB-231 C348(0.69); C96(0.69)  LDD2113  [4]
 LDCM0498  BS-3668 MDA-MB-231 C434(0.62); C269(0.84)  LDD2091  [4]
 LDCM0108  Chloroacetamide HeLa C269(0.00); C434(0.00); C348(0.00); H33(0.00)  LDD0222  [6]
 LDCM0213  Electrophilic fragment 2 MDA-MB-231 C96(0.66)  LDD1702  [4]
 LDCM0107  IAA HeLa C269(0.00); H33(0.00); C434(0.00); H184(0.00)  LDD0221  [6]
 LDCM0022  KB02 22RV1 C236(1.09)  LDD2243  [3]
 LDCM0023  KB03 MDA-MB-231 C96(0.52)  LDD1701  [4]
 LDCM0024  KB05 COLO792 C348(2.82); C236(1.30)  LDD3310  [3]
 LDCM0109  NEM HeLa H325(0.00); H33(0.00); H184(0.00); H65(0.00)  LDD0223  [6]
 LDCM0496  Nucleophilic fragment 11a MDA-MB-231 C348(0.96)  LDD2089  [4]
 LDCM0497  Nucleophilic fragment 11b MDA-MB-231 C348(1.18)  LDD2090  [4]
 LDCM0500  Nucleophilic fragment 13a MDA-MB-231 C434(0.88); C348(0.92)  LDD2093  [4]
 LDCM0504  Nucleophilic fragment 15a MDA-MB-231 C434(1.01); C348(0.64); C269(1.00)  LDD2097  [4]
 LDCM0506  Nucleophilic fragment 16a MDA-MB-231 C348(0.95); C269(0.95)  LDD2099  [4]
 LDCM0507  Nucleophilic fragment 16b MDA-MB-231 C348(0.47); C269(0.41)  LDD2100  [4]
 LDCM0508  Nucleophilic fragment 17a MDA-MB-231 C434(0.86); C348(0.53); C269(0.77); C101(1.53)  LDD2101  [4]
 LDCM0511  Nucleophilic fragment 18b MDA-MB-231 C348(0.45); C269(0.31)  LDD2104  [4]
 LDCM0513  Nucleophilic fragment 19b MDA-MB-231 C269(0.15)  LDD2106  [4]
 LDCM0514  Nucleophilic fragment 20a MDA-MB-231 C434(0.94); C348(0.97); C269(1.01)  LDD2107  [4]
 LDCM0515  Nucleophilic fragment 20b MDA-MB-231 C434(0.51); C348(0.48)  LDD2108  [4]
 LDCM0516  Nucleophilic fragment 21a MDA-MB-231 C434(0.47); C348(0.52); C269(0.75); C96(0.50)  LDD2109  [4]
 LDCM0517  Nucleophilic fragment 21b MDA-MB-231 C348(0.51)  LDD2110  [4]
 LDCM0518  Nucleophilic fragment 22a MDA-MB-231 C434(1.05)  LDD2111  [4]
 LDCM0521  Nucleophilic fragment 23b MDA-MB-231 C348(0.69); C269(0.36)  LDD2114  [4]
 LDCM0522  Nucleophilic fragment 24a MDA-MB-231 C348(0.38)  LDD2115  [4]
 LDCM0526  Nucleophilic fragment 26a MDA-MB-231 C434(1.87); C348(1.76)  LDD2119  [4]
 LDCM0527  Nucleophilic fragment 26b MDA-MB-231 C269(0.82)  LDD2120  [4]
 LDCM0530  Nucleophilic fragment 28a MDA-MB-231 C434(0.75); C348(0.83); C269(1.03); C101(0.82)  LDD2123  [4]
 LDCM0532  Nucleophilic fragment 29a MDA-MB-231 C348(0.74); C269(0.86); C101(0.74)  LDD2125  [4]
 LDCM0534  Nucleophilic fragment 30a MDA-MB-231 C434(1.02); C348(0.89); C269(1.51); C101(0.82)  LDD2127  [4]
 LDCM0535  Nucleophilic fragment 30b MDA-MB-231 C269(0.52)  LDD2128  [4]
 LDCM0536  Nucleophilic fragment 31 MDA-MB-231 C434(1.23); C348(1.02)  LDD2129  [4]
 LDCM0540  Nucleophilic fragment 35 MDA-MB-231 C348(0.39)  LDD2133  [4]
 LDCM0541  Nucleophilic fragment 36 MDA-MB-231 C348(0.30); C101(0.81)  LDD2134  [4]
 LDCM0542  Nucleophilic fragment 37 MDA-MB-231 C434(1.54); C348(1.30)  LDD2135  [4]
 LDCM0543  Nucleophilic fragment 38 MDA-MB-231 C434(1.06); C269(1.18); C101(1.16); C96(1.09)  LDD2136  [4]
 LDCM0544  Nucleophilic fragment 39 MDA-MB-231 C434(1.01); C348(0.92); C269(0.94); C96(0.81)  LDD2137  [4]
 LDCM0211  Nucleophilic fragment 3b MDA-MB-231 C348(1.66)  LDD1700  [4]
 LDCM0546  Nucleophilic fragment 40 MDA-MB-231 C348(0.75); C269(1.06)  LDD2140  [4]
 LDCM0547  Nucleophilic fragment 41 MDA-MB-231 C269(0.53)  LDD2141  [4]
 LDCM0549  Nucleophilic fragment 43 MDA-MB-231 C269(0.62)  LDD2143  [4]
 LDCM0550  Nucleophilic fragment 5a MDA-MB-231 C434(2.53); C348(1.95); C269(2.42)  LDD2144  [4]
 LDCM0551  Nucleophilic fragment 5b MDA-MB-231 C348(0.41)  LDD2145  [4]
 LDCM0552  Nucleophilic fragment 6a MDA-MB-231 C434(0.93); C348(0.85)  LDD2146  [4]
 LDCM0553  Nucleophilic fragment 6b MDA-MB-231 C269(1.57)  LDD2147  [4]
 LDCM0554  Nucleophilic fragment 7a MDA-MB-231 C434(0.38); C348(0.34); C269(0.46); C101(0.59)  LDD2148  [4]
 LDCM0556  Nucleophilic fragment 8a MDA-MB-231 C434(0.44); C269(0.56)  LDD2150  [4]

References

1 Chemoproteomic profiling of kinases in live cells using electrophilic sulfonyl triazole probes. Chem Sci. 2021 Jan 21;12(9):3295-3307. doi: 10.1039/d0sc06623k.
2 2H-Azirine-Based Reagents for Chemoselective Bioconjugation at Carboxyl Residues Inside Live Cells. J Am Chem Soc. 2020 Apr 1;142(13):6051-6059. doi: 10.1021/jacs.9b12116. Epub 2020 Mar 23.
3 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
4 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
5 Chemoproteomic capture of RNA binding activity in living cells. Nat Commun. 2023 Oct 7;14(1):6282. doi: 10.1038/s41467-023-41844-z.
Mass spectrometry data entry: PXD044625
6 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
7 Design and synthesis of minimalist terminal alkyne-containing diazirine photo-crosslinkers and their incorporation into kinase inhibitors for cell- and tissue-based proteome profiling. Angew Chem Int Ed Engl. 2013 Aug 12;52(33):8551-6. doi: 10.1002/anie.201300683. Epub 2013 Jun 10.