Details of the Target

General Information of Target

Target ID LDTP14124
Target Name Pleckstrin homology-like domain family A member 3 (PHLDA3)
Gene Name PHLDA3
Gene ID 23612
Synonyms
TIH1; Pleckstrin homology-like domain family A member 3; TDAG51/Ipl homolog 1
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MKRAHPEYSSSDSELDETIEVEKESADENGNLSSALGSMSPTTSSQILARKRRRGIIEKR
RRDRINNSLSELRRLVPSAFEKQGSAKLEKAEILQMTVDHLKMLHTAGGKGYFDAHALAM
DYRSLGFRECLAEVARYLSIIEGLDASDPLRVRLVSHLNNYASQREAASGAHAGLGHIPW
GTVFGHHPHIAHPLLLPQNGHGNAGTTASPTEPHHQGRLGSAHPEAPALRAPPSGSLGPV
LPVVTSASKLSPPLLSSVASLSAFPFSFGSFHLLSPNALSPSAPTQAANLGKPYRPWGTE
IGAF
Target Bioclass
Other
Family
PHLDA3 family
Subcellular location
Cytoplasm
Function
p53/TP53-regulated repressor of Akt/AKT1 signaling. Represses AKT1 by preventing AKT1-binding to membrane lipids, thereby inhibiting AKT1 translocation to the cellular membrane and activation. Contributes to p53/TP53-dependent apoptosis by repressing AKT1 activity. Its direct transcription regulation by p53/TP53 may explain how p53/TP53 can negatively regulate AKT1. May act as a tumor suppressor.
Uniprot ID
Q9Y5J5
Ensemble ID
ENST00000367309.1
HGNC ID
HGNC:8934

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 8 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
STPyne
 Probe Info  		K51(6.67)  LDD0277  [1]
IPM
 Probe Info  		N.A.  LDD0241  [2]
DBIA
 Probe Info  		C63(1.20)  LDD3315  [3]
IA-alkyne
 Probe Info  		N.A.  LDD0165  [4]
TFBX
 Probe Info  		C63(0.00); C31(0.00)  LDD0027  [5]
WYneO
 Probe Info  		N.A.  LDD0022  [6]
W1
 Probe Info  		N.A.  LDD0236  [2]
NAIA_5
 Probe Info  		C63(0.00); C31(0.00)  LDD2223  [7]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0215  AC10 HEK-293T C63(0.96)  LDD1508  [8]
 LDCM0259  AC14 HEK-293T C63(1.03)  LDD1512  [8]
 LDCM0270  AC15 HEK-293T C63(1.07)  LDD1513  [8]
 LDCM0277  AC18 HEK-293T C63(0.96)  LDD1516  [8]
 LDCM0279  AC2 HEK-293T C63(0.97)  LDD1518  [8]
 LDCM0281  AC21 HEK-293T C63(0.88)  LDD1520  [8]
 LDCM0282  AC22 HEK-293T C63(0.99)  LDD1521  [8]
 LDCM0283  AC23 HEK-293T C63(1.10)  LDD1522  [8]
 LDCM0284  AC24 HEK-293T C63(0.97); C87(1.09)  LDD1523  [8]
 LDCM0286  AC26 HEK-293T C63(1.06)  LDD1525  [8]
 LDCM0289  AC29 HEK-293T C63(0.97)  LDD1528  [8]
 LDCM0291  AC30 HEK-293T C63(1.01)  LDD1530  [8]
 LDCM0292  AC31 HEK-293T C63(1.14)  LDD1531  [8]
 LDCM0293  AC32 HEK-293T C63(0.92); C87(1.04)  LDD1532  [8]
 LDCM0295  AC34 HEK-293T C63(1.06)  LDD1534  [8]
 LDCM0298  AC37 HEK-293T C63(1.01)  LDD1537  [8]
 LDCM0299  AC38 HEK-293T C63(0.99)  LDD1538  [8]
 LDCM0300  AC39 HEK-293T C63(1.14)  LDD1539  [8]
 LDCM0302  AC40 HEK-293T C63(0.98); C87(1.12)  LDD1541  [8]
 LDCM0304  AC42 HEK-293T C63(0.99)  LDD1543  [8]
 LDCM0307  AC45 HEK-293T C63(0.98)  LDD1546  [8]
 LDCM0308  AC46 HEK-293T C63(1.00)  LDD1547  [8]
 LDCM0309  AC47 HEK-293T C63(1.10)  LDD1548  [8]
 LDCM0310  AC48 HEK-293T C63(0.94); C87(1.07)  LDD1549  [8]
 LDCM0312  AC5 HEK-293T C63(0.94)  LDD1551  [8]
 LDCM0313  AC50 HEK-293T C63(1.06)  LDD1552  [8]
 LDCM0316  AC53 HEK-293T C63(0.97)  LDD1555  [8]
 LDCM0317  AC54 HEK-293T C63(1.01)  LDD1556  [8]
 LDCM0318  AC55 HEK-293T C63(1.07)  LDD1557  [8]
 LDCM0319  AC56 HEK-293T C63(0.94); C87(1.08)  LDD1558  [8]
 LDCM0321  AC58 HEK-293T C63(1.03)  LDD1560  [8]
 LDCM0323  AC6 HEK-293T C63(0.97)  LDD1562  [8]
 LDCM0325  AC61 HEK-293T C63(0.88)  LDD1564  [8]
 LDCM0326  AC62 HEK-293T C63(0.97)  LDD1565  [8]
 LDCM0327  AC63 HEK-293T C63(1.14)  LDD1566  [8]
 LDCM0328  AC64 HEK-293T C63(0.90); C87(1.04)  LDD1567  [8]
 LDCM0334  AC7 HEK-293T C63(1.12)  LDD1568  [8]
 LDCM0345  AC8 HEK-293T C63(1.01); C87(1.01)  LDD1569  [8]
 LDCM0248  AKOS034007472 HEK-293T C63(0.99)  LDD1511  [8]
 LDCM0275  AKOS034007705 HEK-293T C63(0.96); C87(1.10)  LDD1514  [8]
 LDCM0632  CL-Sc Hep-G2 C63(0.92)  LDD2227  [7]
 LDCM0368  CL10 HEK-293T C63(0.93)  LDD1572  [8]
 LDCM0369  CL100 HEK-293T C87(0.93)  LDD1573  [8]
 LDCM0371  CL102 HEK-293T C63(0.92)  LDD1575  [8]
 LDCM0372  CL103 HEK-293T C63(1.01)  LDD1576  [8]
 LDCM0373  CL104 HEK-293T C87(0.77)  LDD1577  [8]
 LDCM0375  CL106 HEK-293T C63(0.85)  LDD1579  [8]
 LDCM0376  CL107 HEK-293T C63(1.09)  LDD1580  [8]
 LDCM0377  CL108 HEK-293T C87(1.04)  LDD1581  [8]
 LDCM0379  CL11 HEK-293T C63(1.15)  LDD1583  [8]
 LDCM0380  CL110 HEK-293T C63(0.89)  LDD1584  [8]
 LDCM0381  CL111 HEK-293T C63(0.96)  LDD1585  [8]
 LDCM0382  CL112 HEK-293T C87(1.08)  LDD1586  [8]
 LDCM0384  CL114 HEK-293T C63(0.91)  LDD1588  [8]
 LDCM0385  CL115 HEK-293T C63(1.03)  LDD1589  [8]
 LDCM0386  CL116 HEK-293T C87(1.09)  LDD1590  [8]
 LDCM0388  CL118 HEK-293T C63(0.93)  LDD1592  [8]
 LDCM0389  CL119 HEK-293T C63(0.99)  LDD1593  [8]
 LDCM0390  CL12 HEK-293T C63(1.13); C87(1.13)  LDD1594  [8]
 LDCM0391  CL120 HEK-293T C87(1.15)  LDD1595  [8]
 LDCM0393  CL122 HEK-293T C63(0.94)  LDD1597  [8]
 LDCM0394  CL123 HEK-293T C63(0.90)  LDD1598  [8]
 LDCM0395  CL124 HEK-293T C87(1.10)  LDD1599  [8]
 LDCM0397  CL126 HEK-293T C63(0.99)  LDD1601  [8]
 LDCM0398  CL127 HEK-293T C63(0.99)  LDD1602  [8]
 LDCM0399  CL128 HEK-293T C87(1.17)  LDD1603  [8]
 LDCM0401  CL14 HEK-293T C63(1.00)  LDD1605  [8]
 LDCM0402  CL15 HEK-293T C63(0.99)  LDD1606  [8]
 LDCM0403  CL16 HEK-293T C87(1.03)  LDD1607  [8]
 LDCM0405  CL18 HEK-293T C63(1.09)  LDD1609  [8]
 LDCM0407  CL2 HEK-293T C63(1.11)  LDD1611  [8]
 LDCM0409  CL21 HEK-293T C63(0.98)  LDD1613  [8]
 LDCM0410  CL22 HEK-293T C63(1.20)  LDD1614  [8]
 LDCM0411  CL23 HEK-293T C63(1.27)  LDD1615  [8]
 LDCM0412  CL24 HEK-293T C63(1.03); C87(1.15)  LDD1616  [8]
 LDCM0414  CL26 HEK-293T C63(0.99)  LDD1618  [8]
 LDCM0415  CL27 HEK-293T C63(0.99)  LDD1619  [8]
 LDCM0416  CL28 HEK-293T C87(0.98)  LDD1620  [8]
 LDCM0418  CL3 HEK-293T C63(1.10)  LDD1622  [8]
 LDCM0419  CL30 HEK-293T C63(1.02)  LDD1623  [8]
 LDCM0422  CL33 HEK-293T C63(0.89)  LDD1626  [8]
 LDCM0423  CL34 HEK-293T C63(1.10)  LDD1627  [8]
 LDCM0424  CL35 HEK-293T C63(1.25)  LDD1628  [8]
 LDCM0425  CL36 HEK-293T C63(1.03); C87(1.02)  LDD1629  [8]
 LDCM0428  CL39 HEK-293T C63(1.03)  LDD1632  [8]
 LDCM0429  CL4 HEK-293T C87(1.02)  LDD1633  [8]
 LDCM0430  CL40 HEK-293T C87(1.09)  LDD1634  [8]
 LDCM0432  CL42 HEK-293T C63(1.02)  LDD1636  [8]
 LDCM0435  CL45 HEK-293T C63(0.94)  LDD1639  [8]
 LDCM0436  CL46 HEK-293T C63(1.19)  LDD1640  [8]
 LDCM0437  CL47 HEK-293T C63(1.09)  LDD1641  [8]
 LDCM0438  CL48 HEK-293T C63(0.90); C87(1.03)  LDD1642  [8]
 LDCM0441  CL50 HEK-293T C63(0.90)  LDD1645  [8]
 LDCM0443  CL52 HEK-293T C87(1.06)  LDD1646  [8]
 LDCM0445  CL54 HEK-293T C63(0.97)  LDD1648  [8]
 LDCM0448  CL57 HEK-293T C63(0.95)  LDD1651  [8]
 LDCM0449  CL58 HEK-293T C63(1.18)  LDD1652  [8]
 LDCM0450  CL59 HEK-293T C63(1.19)  LDD1653  [8]
 LDCM0451  CL6 HEK-293T C63(1.13)  LDD1654  [8]
 LDCM0452  CL60 HEK-293T C63(1.01); C87(1.28)  LDD1655  [8]
 LDCM0454  CL62 HEK-293T C63(0.95)  LDD1657  [8]
 LDCM0455  CL63 HEK-293T C63(1.04)  LDD1658  [8]
 LDCM0456  CL64 HEK-293T C87(1.25)  LDD1659  [8]
 LDCM0458  CL66 HEK-293T C63(1.00)  LDD1661  [8]
 LDCM0461  CL69 HEK-293T C63(0.93)  LDD1664  [8]
 LDCM0463  CL70 HEK-293T C63(1.15)  LDD1666  [8]
 LDCM0464  CL71 HEK-293T C63(1.14)  LDD1667  [8]
 LDCM0465  CL72 HEK-293T C63(0.97); C87(1.05)  LDD1668  [8]
 LDCM0467  CL74 HEK-293T C63(1.00)  LDD1670  [8]
 LDCM0469  CL76 HEK-293T C87(0.97)  LDD1672  [8]
 LDCM0471  CL78 HEK-293T C63(1.04)  LDD1674  [8]
 LDCM0475  CL81 HEK-293T C63(0.95)  LDD1678  [8]
 LDCM0476  CL82 HEK-293T C63(1.03)  LDD1679  [8]
 LDCM0477  CL83 HEK-293T C63(1.00)  LDD1680  [8]
 LDCM0478  CL84 HEK-293T C63(1.04); C87(1.12)  LDD1681  [8]
 LDCM0480  CL86 HEK-293T C63(0.91)  LDD1683  [8]
 LDCM0481  CL87 HEK-293T C63(0.99)  LDD1684  [8]
 LDCM0482  CL88 HEK-293T C87(1.07)  LDD1685  [8]
 LDCM0484  CL9 HEK-293T C63(0.94)  LDD1687  [8]
 LDCM0485  CL90 HEK-293T C63(0.94)  LDD1688  [8]
 LDCM0488  CL93 HEK-293T C63(0.91)  LDD1691  [8]
 LDCM0489  CL94 HEK-293T C63(1.10)  LDD1692  [8]
 LDCM0490  CL95 HEK-293T C63(1.02)  LDD1693  [8]
 LDCM0491  CL96 HEK-293T C63(0.98); C87(1.21)  LDD1694  [8]
 LDCM0493  CL98 HEK-293T C63(0.92)  LDD1696  [8]
 LDCM0494  CL99 HEK-293T C63(0.92)  LDD1697  [8]
 LDCM0495  E2913 HEK-293T C63(1.09)  LDD1698  [8]
 LDCM0468  Fragment33 HEK-293T C63(0.93)  LDD1671  [8]
 LDCM0427  Fragment51 HEK-293T C63(0.95)  LDD1631  [8]
 LDCM0022  KB02 HEK-293T C63(1.02)  LDD1492  [8]
 LDCM0023  KB03 HEK-293T C63(0.91)  LDD1497  [8]
 LDCM0024  KB05 IGR39 C63(1.20)  LDD3315  [3]

References

1 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
2 Oxidant-Induced Bioconjugation for Protein Labeling in Live Cells. ACS Chem Biol. 2023 Jan 20;18(1):112-122. doi: 10.1021/acschembio.2c00740. Epub 2022 Dec 21.
3 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
4 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
5 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
6 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
7 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
8 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402