Details of the Target

General Information of Target

Target ID LDTP13310
Target Name Solute carrier family 12 member 6 (SLC12A6)
Gene Name SLC12A6
Gene ID 9990
Synonyms
KCC3; Solute carrier family 12 member 6; Electroneutral potassium-chloride cotransporter 3; K-Cl cotransporter 3
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MAENSGRAGKSSGSGAGKGAVSAEQVIAGFNRLRQEQRGLASKAAELEMELNEHSLVIDT
LKEVDETRKCYRMVGGVLVERTVKEVLPALENNKEQIQKIIETLTQQLQAKGKELNEFRE
KHNIRLMGEDEKPAAKENSEGAGAKASSAGVLVS
Target Type
Literature-reported
Target Bioclass
Transporter and channel
Family
SLC12A transporter family
Subcellular location
Cell membrane
Function
[Isoform 1]: Mediates electroneutral potassium-chloride cotransport when activated by cell swelling. May contribute to cell volume homeostasis in single cells.; [Isoform 2]: Mediates electroneutral potassium-chloride cotransport when activated by cell swelling. May contribute to cell volume homeostasis in single cells (Probable).; [Isoform 3]: Mediates electroneutral potassium-chloride cotransport when activated by cell swelling. May contribute to cell volume homeostasis in single cells (Probable).; [Isoform 4]: Mediates electroneutral potassium-chloride cotransport when activated by cell swelling. May contribute to cell volume homeostasis in single cells (Probable).; [Isoform 5]: Mediates electroneutral potassium-chloride cotransport when activated by cell swelling. May contribute to cell volume homeostasis in single cells (Probable).; [Isoform 6]: Mediates electroneutral potassium-chloride cotransport when activated by cell swelling. May contribute to cell volume homeostasis in single cells (Probable).
TTD ID
T75545
Uniprot ID
Q9UHW9
DrugMap ID
TT8DFHE
Ensemble ID
ENST00000290209.9
HGNC ID
HGNC:10914

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
22RV1 SNV: p.L469S .
786O SNV: p.W406R .
HUH28 SNV: p.E1039K .
JHH7 SNV: p.F291S .
JM1 SNV: p.A872T .
JURKAT SNV: p.R81W .
LNCaP clone FGC SNV: p.N799K .
RL952 Deletion: p.M922CfsTer9 .
SNGM SNV: p.P33L .
SNU1 SNV: p.I1094M .
SW1116 SNV: p.F577L .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 3 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C390(3.02)  LDD3312  [1]
ATP probe
 Probe Info  		N.A.  LDD0199  [2]
IA-alkyne
 Probe Info  		N.A.  LDD0162  [3]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0214  AC1 HEK-293T C390(1.00)  LDD1507  [4]
 LDCM0215  AC10 HEK-293T C390(0.97)  LDD1508  [4]
 LDCM0226  AC11 HEK-293T C390(0.94)  LDD1509  [4]
 LDCM0259  AC14 HEK-293T C390(1.09)  LDD1512  [4]
 LDCM0270  AC15 HEK-293T C390(1.18)  LDD1513  [4]
 LDCM0276  AC17 HEK-293T C390(1.06)  LDD1515  [4]
 LDCM0277  AC18 HEK-293T C390(0.98)  LDD1516  [4]
 LDCM0278  AC19 HEK-293T C390(1.09)  LDD1517  [4]
 LDCM0279  AC2 HEK-293T C390(1.05)  LDD1518  [4]
 LDCM0282  AC22 HEK-293T C390(1.04)  LDD1521  [4]
 LDCM0283  AC23 HEK-293T C390(1.04)  LDD1522  [4]
 LDCM0284  AC24 HEK-293T C390(1.05)  LDD1523  [4]
 LDCM0285  AC25 HEK-293T C390(1.12)  LDD1524  [4]
 LDCM0286  AC26 HEK-293T C390(1.02)  LDD1525  [4]
 LDCM0287  AC27 HEK-293T C390(0.99)  LDD1526  [4]
 LDCM0290  AC3 HEK-293T C390(1.06)  LDD1529  [4]
 LDCM0291  AC30 HEK-293T C390(1.10)  LDD1530  [4]
 LDCM0292  AC31 HEK-293T C390(1.05)  LDD1531  [4]
 LDCM0293  AC32 HEK-293T C390(0.99)  LDD1532  [4]
 LDCM0294  AC33 HEK-293T C390(0.98)  LDD1533  [4]
 LDCM0295  AC34 HEK-293T C390(1.00)  LDD1534  [4]
 LDCM0296  AC35 HEK-293T C390(0.94)  LDD1535  [4]
 LDCM0299  AC38 HEK-293T C390(1.13)  LDD1538  [4]
 LDCM0300  AC39 HEK-293T C390(1.08)  LDD1539  [4]
 LDCM0302  AC40 HEK-293T C390(0.98)  LDD1541  [4]
 LDCM0303  AC41 HEK-293T C390(0.96)  LDD1542  [4]
 LDCM0304  AC42 HEK-293T C390(1.10)  LDD1543  [4]
 LDCM0305  AC43 HEK-293T C390(1.05)  LDD1544  [4]
 LDCM0308  AC46 HEK-293T C390(1.05)  LDD1547  [4]
 LDCM0309  AC47 HEK-293T C390(1.12)  LDD1548  [4]
 LDCM0310  AC48 HEK-293T C390(0.99)  LDD1549  [4]
 LDCM0311  AC49 HEK-293T C390(1.04)  LDD1550  [4]
 LDCM0313  AC50 HEK-293T C390(1.02)  LDD1552  [4]
 LDCM0314  AC51 HEK-293T C390(1.00)  LDD1553  [4]
 LDCM0317  AC54 HEK-293T C390(0.99)  LDD1556  [4]
 LDCM0318  AC55 HEK-293T C390(1.08)  LDD1557  [4]
 LDCM0319  AC56 HEK-293T C390(0.95)  LDD1558  [4]
 LDCM0320  AC57 HEK-293T C390(0.92)  LDD1559  [4]
 LDCM0321  AC58 HEK-293T C390(1.07)  LDD1560  [4]
 LDCM0322  AC59 HEK-293T C390(1.01)  LDD1561  [4]
 LDCM0323  AC6 HEK-293T C390(1.06)  LDD1562  [4]
 LDCM0326  AC62 HEK-293T C390(1.05)  LDD1565  [4]
 LDCM0327  AC63 HEK-293T C390(1.07)  LDD1566  [4]
 LDCM0328  AC64 HEK-293T C390(1.00)  LDD1567  [4]
 LDCM0334  AC7 HEK-293T C390(1.01)  LDD1568  [4]
 LDCM0345  AC8 HEK-293T C390(0.95)  LDD1569  [4]
 LDCM0356  AKOS034007680 HEK-293T C390(1.03)  LDD1570  [4]
 LDCM0275  AKOS034007705 HEK-293T C390(1.05)  LDD1514  [4]
 LDCM0367  CL1 HEK-293T C390(1.11)  LDD1571  [4]
 LDCM0368  CL10 HEK-293T C390(1.34)  LDD1572  [4]
 LDCM0369  CL100 HEK-293T C390(1.01)  LDD1573  [4]
 LDCM0370  CL101 HEK-293T C390(0.88)  LDD1574  [4]
 LDCM0371  CL102 HEK-293T C390(1.07)  LDD1575  [4]
 LDCM0373  CL104 HEK-293T C390(0.96)  LDD1577  [4]
 LDCM0374  CL105 HEK-293T C390(0.93)  LDD1578  [4]
 LDCM0375  CL106 HEK-293T C390(0.97)  LDD1579  [4]
 LDCM0377  CL108 HEK-293T C390(0.97)  LDD1581  [4]
 LDCM0378  CL109 HEK-293T C390(0.97)  LDD1582  [4]
 LDCM0379  CL11 HEK-293T C390(1.09)  LDD1583  [4]
 LDCM0380  CL110 HEK-293T C390(0.99)  LDD1584  [4]
 LDCM0382  CL112 HEK-293T C390(0.93)  LDD1586  [4]
 LDCM0383  CL113 HEK-293T C390(1.01)  LDD1587  [4]
 LDCM0384  CL114 HEK-293T C390(1.09)  LDD1588  [4]
 LDCM0386  CL116 HEK-293T C390(1.05)  LDD1590  [4]
 LDCM0387  CL117 HEK-293T C390(0.87)  LDD1591  [4]
 LDCM0388  CL118 HEK-293T C390(1.03)  LDD1592  [4]
 LDCM0390  CL12 HEK-293T C390(1.08)  LDD1594  [4]
 LDCM0391  CL120 HEK-293T C390(1.05)  LDD1595  [4]
 LDCM0392  CL121 HEK-293T C390(0.92)  LDD1596  [4]
 LDCM0393  CL122 HEK-293T C390(1.19)  LDD1597  [4]
 LDCM0395  CL124 HEK-293T C390(1.02)  LDD1599  [4]
 LDCM0396  CL125 HEK-293T C390(0.91)  LDD1600  [4]
 LDCM0397  CL126 HEK-293T C390(0.99)  LDD1601  [4]
 LDCM0399  CL128 HEK-293T C390(1.00)  LDD1603  [4]
 LDCM0400  CL13 HEK-293T C390(1.05)  LDD1604  [4]
 LDCM0401  CL14 HEK-293T C390(1.06)  LDD1605  [4]
 LDCM0403  CL16 HEK-293T C390(1.07)  LDD1607  [4]
 LDCM0404  CL17 HEK-293T C390(0.99)  LDD1608  [4]
 LDCM0405  CL18 HEK-293T C390(1.08)  LDD1609  [4]
 LDCM0406  CL19 HEK-293T C390(0.96)  LDD1610  [4]
 LDCM0407  CL2 HEK-293T C390(1.07)  LDD1611  [4]
 LDCM0410  CL22 HEK-293T C390(1.14)  LDD1614  [4]
 LDCM0411  CL23 HEK-293T C390(1.13)  LDD1615  [4]
 LDCM0412  CL24 HEK-293T C390(1.13)  LDD1616  [4]
 LDCM0413  CL25 HEK-293T C390(0.96)  LDD1617  [4]
 LDCM0414  CL26 HEK-293T C390(0.85)  LDD1618  [4]
 LDCM0416  CL28 HEK-293T C390(1.03)  LDD1620  [4]
 LDCM0417  CL29 HEK-293T C390(1.02)  LDD1621  [4]
 LDCM0419  CL30 HEK-293T C390(0.99)  LDD1623  [4]
 LDCM0420  CL31 HEK-293T C390(1.07)  LDD1624  [4]
 LDCM0423  CL34 HEK-293T C390(1.14)  LDD1627  [4]
 LDCM0424  CL35 HEK-293T C390(1.13)  LDD1628  [4]
 LDCM0425  CL36 HEK-293T C390(1.11)  LDD1629  [4]
 LDCM0426  CL37 HEK-293T C390(0.88)  LDD1630  [4]
 LDCM0429  CL4 HEK-293T C390(1.16)  LDD1633  [4]
 LDCM0430  CL40 HEK-293T C390(1.02)  LDD1634  [4]
 LDCM0431  CL41 HEK-293T C390(0.96)  LDD1635  [4]
 LDCM0432  CL42 HEK-293T C390(0.94)  LDD1636  [4]
 LDCM0433  CL43 HEK-293T C390(1.06)  LDD1637  [4]
 LDCM0436  CL46 HEK-293T C390(1.11)  LDD1640  [4]
 LDCM0437  CL47 HEK-293T C390(1.16)  LDD1641  [4]
 LDCM0438  CL48 HEK-293T C390(1.14)  LDD1642  [4]
 LDCM0439  CL49 HEK-293T C390(1.07)  LDD1643  [4]
 LDCM0440  CL5 HEK-293T C390(0.94)  LDD1644  [4]
 LDCM0441  CL50 HEK-293T C390(1.06)  LDD1645  [4]
 LDCM0443  CL52 HEK-293T C390(1.04)  LDD1646  [4]
 LDCM0444  CL53 HEK-293T C390(1.19)  LDD1647  [4]
 LDCM0445  CL54 HEK-293T C390(1.09)  LDD1648  [4]
 LDCM0446  CL55 HEK-293T C390(1.01)  LDD1649  [4]
 LDCM0449  CL58 HEK-293T C390(1.19)  LDD1652  [4]
 LDCM0450  CL59 HEK-293T C390(1.21)  LDD1653  [4]
 LDCM0451  CL6 HEK-293T C390(1.20)  LDD1654  [4]
 LDCM0452  CL60 HEK-293T C390(1.04)  LDD1655  [4]
 LDCM0453  CL61 HEK-293T C390(0.86)  LDD1656  [4]
 LDCM0454  CL62 HEK-293T C390(1.07)  LDD1657  [4]
 LDCM0456  CL64 HEK-293T C390(0.96)  LDD1659  [4]
 LDCM0457  CL65 HEK-293T C390(1.08)  LDD1660  [4]
 LDCM0458  CL66 HEK-293T C390(1.03)  LDD1661  [4]
 LDCM0459  CL67 HEK-293T C390(1.11)  LDD1662  [4]
 LDCM0462  CL7 HEK-293T C390(1.06)  LDD1665  [4]
 LDCM0463  CL70 HEK-293T C390(1.17)  LDD1666  [4]
 LDCM0464  CL71 HEK-293T C390(1.14)  LDD1667  [4]
 LDCM0465  CL72 HEK-293T C390(0.98)  LDD1668  [4]
 LDCM0466  CL73 HEK-293T C390(1.07)  LDD1669  [4]
 LDCM0467  CL74 HEK-293T C390(1.05)  LDD1670  [4]
 LDCM0469  CL76 HEK-293T C390(1.06)  LDD1672  [4]
 LDCM0470  CL77 HEK-293T C390(1.12)  LDD1673  [4]
 LDCM0471  CL78 HEK-293T C390(0.92)  LDD1674  [4]
 LDCM0472  CL79 HEK-293T C390(1.10)  LDD1675  [4]
 LDCM0476  CL82 HEK-293T C390(1.20)  LDD1679  [4]
 LDCM0477  CL83 HEK-293T C390(1.15)  LDD1680  [4]
 LDCM0478  CL84 HEK-293T C390(0.95)  LDD1681  [4]
 LDCM0479  CL85 HEK-293T C390(0.83)  LDD1682  [4]
 LDCM0480  CL86 HEK-293T C390(1.13)  LDD1683  [4]
 LDCM0482  CL88 HEK-293T C390(0.92)  LDD1685  [4]
 LDCM0483  CL89 HEK-293T C390(1.05)  LDD1686  [4]
 LDCM0485  CL90 HEK-293T C390(1.20)  LDD1688  [4]
 LDCM0486  CL91 HEK-293T C390(0.99)  LDD1689  [4]
 LDCM0489  CL94 HEK-293T C390(1.15)  LDD1692  [4]
 LDCM0490  CL95 HEK-293T C390(1.32)  LDD1693  [4]
 LDCM0491  CL96 HEK-293T C390(1.11)  LDD1694  [4]
 LDCM0492  CL97 HEK-293T C390(0.79)  LDD1695  [4]
 LDCM0493  CL98 HEK-293T C390(1.08)  LDD1696  [4]
 LDCM0427  Fragment51 HEK-293T C390(0.96)  LDD1631  [4]
 LDCM0022  KB02 A2058 C390(2.65)  LDD2253  [1]
 LDCM0023  KB03 A101D C390(2.71)  LDD2667  [1]
 LDCM0024  KB05 HMCB C390(3.02)  LDD3312  [1]

The Interaction Atlas With This Target

The Drug(s) Related To This Target

Approved
Click To Hide/Show 1 Drug(s) Interacting with This Target
Drug Name Drug Type External ID
Potassium Chloride Small molecular drug DB00761
Investigative
Click To Hide/Show 1 Drug(s) Interacting with This Target
Drug Name Drug Type External ID
Dioa Small molecular drug D0K9KZ

References

1 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
2 Targeted Proteomic Approaches for Proteome-Wide Characterizations of the AMP-Binding Capacities of Kinases. J Proteome Res. 2022 Aug 5;21(8):2063-2070. doi: 10.1021/acs.jproteome.2c00225. Epub 2022 Jul 12.
3 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
4 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402