Details of the Target

General Information of Target

Target ID LDTP13255
Target Name Max-like protein X (MLX)
Gene Name MLX
Gene ID 6945
Synonyms
BHLHD13; TCFL4; Max-like protein X; Class D basic helix-loop-helix protein 13; bHLHd13; Max-like bHLHZip protein; Protein BigMax; Transcription factor-like protein 4
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MEGESVKLSSQTLIQAGDDEKNQRTITVNPAHMGKAFKVMNELRSKQLLCDVMIVAEDVE
IEAHRVVLAACSPYFCAMFTGDMSESKAKKIEIKDVDGQTLSKLIDYIYTAEIEVTEENV
QVLLPAASLLQLMDVRQNCCDFLQSQLHPTNCLGIRAFADVHTCTDLLQQANAYAEQHFP
EVMLGEEFLSLSLDQVCSLISSDKLTVSSEEKVFEAVISWINYEKETRLEHMAKLMEHVR
LPLLPRDYLVQTVEEEALIKNNNTCKDFLIEAMKYHLLPLDQRLLIKNPRTKPRTPVSLP
KVMIVVGGQAPKAIRSVECYDFEEDRWDQIAELPSRRCRAGVVFMAGHVYAVGGFNGSLR
VRTVDVYDGVKDQWTSIASMQERRSTLGAAVLNDLLYAVGGFDGSTGLASVEAYSYKTNE
WFFVAPMNTRRSSVGVGVVEGKLYAVGGYDGASRQCLSTVEQYNPATNEWIYVADMSTRR
SGAGVGVLSGQLYATGGHDGPLVRKSVEVYDPGTNTWKQVADMNMCRRNAGVCAVNGLLY
VVGGDDGSCNLASVEYYNPVTDKWTLLPTNMSTGRSYAGVAVIHKSL
Target Bioclass
Transcription factor
Subcellular location
Cytoplasm; Nucleus
Function
Transcription regulator. Forms a sequence-specific DNA-binding protein complex with MAD1, MAD4, MNT, WBSCR14 and MLXIP which recognizes the core sequence 5'-CACGTG-3'. The TCFL4-MAD1, TCFL4-MAD4, TCFL4-WBSCR14 complexes are transcriptional repressors. Plays a role in transcriptional activation of glycolytic target genes. Involved in glucose-responsive gene regulation.
Uniprot ID
Q9UH92
Ensemble ID
ENST00000246912.8
HGNC ID
HGNC:11645
ChEMBL ID
CHEMBL2062357

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 7 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
JZ128-DTB
 Probe Info  		N.A.  LDD0462  [1]
IA-alkyne
 Probe Info  		C159(6.28)  LDD1709  [2]
DBIA
 Probe Info  		C159(1.20)  LDD1514  [3]
IPM
 Probe Info  		N.A.  LDD2156  [4]
VSF
 Probe Info  		N.A.  LDD0007  [5]
Phosphinate-6
 Probe Info  		N.A.  LDD0018  [6]
AOyne
 Probe Info  		15.00  LDD0443  [7]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0284  AC24 HEK-293T C159(0.98)  LDD1523  [3]
 LDCM0293  AC32 HEK-293T C159(1.15)  LDD1532  [3]
 LDCM0302  AC40 HEK-293T C159(1.28)  LDD1541  [3]
 LDCM0310  AC48 HEK-293T C159(1.01)  LDD1549  [3]
 LDCM0319  AC56 HEK-293T C159(1.08)  LDD1558  [3]
 LDCM0328  AC64 HEK-293T C159(0.97)  LDD1567  [3]
 LDCM0345  AC8 HEK-293T C159(1.30)  LDD1569  [3]
 LDCM0275  AKOS034007705 HEK-293T C159(1.20)  LDD1514  [3]
 LDCM0369  CL100 HEK-293T C159(0.93)  LDD1573  [3]
 LDCM0373  CL104 HEK-293T C159(1.25)  LDD1577  [3]
 LDCM0377  CL108 HEK-293T C159(0.90)  LDD1581  [3]
 LDCM0382  CL112 HEK-293T C159(1.13)  LDD1586  [3]
 LDCM0386  CL116 HEK-293T C159(1.10)  LDD1590  [3]
 LDCM0390  CL12 HEK-293T C159(1.14)  LDD1594  [3]
 LDCM0391  CL120 HEK-293T C159(1.15)  LDD1595  [3]
 LDCM0395  CL124 HEK-293T C159(1.13)  LDD1599  [3]
 LDCM0399  CL128 HEK-293T C159(1.22)  LDD1603  [3]
 LDCM0403  CL16 HEK-293T C159(1.00)  LDD1607  [3]
 LDCM0412  CL24 HEK-293T C159(1.20)  LDD1616  [3]
 LDCM0416  CL28 HEK-293T C159(1.36)  LDD1620  [3]
 LDCM0425  CL36 HEK-293T C159(1.04)  LDD1629  [3]
 LDCM0429  CL4 HEK-293T C159(1.13)  LDD1633  [3]
 LDCM0430  CL40 HEK-293T C159(1.04)  LDD1634  [3]
 LDCM0438  CL48 HEK-293T C159(0.83)  LDD1642  [3]
 LDCM0443  CL52 HEK-293T C159(1.23)  LDD1646  [3]
 LDCM0452  CL60 HEK-293T C159(1.26)  LDD1655  [3]
 LDCM0456  CL64 HEK-293T C159(1.21)  LDD1659  [3]
 LDCM0465  CL72 HEK-293T C159(1.44)  LDD1668  [3]
 LDCM0469  CL76 HEK-293T C159(1.24)  LDD1672  [3]
 LDCM0478  CL84 HEK-293T C159(0.97)  LDD1681  [3]
 LDCM0482  CL88 HEK-293T C159(1.32)  LDD1685  [3]
 LDCM0491  CL96 HEK-293T C159(1.16)  LDD1694  [3]
 LDCM0625  F8 Ramos C159(1.20)  LDD2187  [8]
 LDCM0575  Fragment13 Ramos C159(0.73)  LDD2192  [8]
 LDCM0580  Fragment21 Ramos C159(0.57)  LDD2195  [8]
 LDCM0582  Fragment23 Ramos C159(0.56)  LDD2196  [8]
 LDCM0578  Fragment27 Ramos C159(0.46)  LDD2197  [8]
 LDCM0586  Fragment28 Ramos C159(2.41)  LDD2198  [8]
 LDCM0588  Fragment30 Ramos C159(0.67)  LDD2199  [8]
 LDCM0589  Fragment31 Ramos C159(0.80)  LDD2200  [8]
 LDCM0468  Fragment33 Ramos C159(1.28)  LDD2202  [8]
 LDCM0614  Fragment56 Ramos C159(0.91)  LDD2205  [8]
 LDCM0179  JZ128 PC-3 N.A.  LDD0462  [1]
 LDCM0024  KB05 T cell C159(6.28)  LDD1709  [2]
 LDCM0628  OTUB2-COV-1 HEK-293T C159(0.51)  LDD2207  [9]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
E3 ubiquitin-protein ligase LNX (LNX1) . Q8TBB1
Transporter and channel
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
AP-4 complex accessory subunit Tepsin (TEPSIN) . Q96N21
Transcription factor
Click To Hide/Show 3 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Transcription regulator protein BACH2 (BACH2) BZIP family Q9BYV9
Zinc finger protein 620 (ZNF620) Krueppel C2H2-type zinc-finger protein family Q6ZNG0
Homeobox protein MOX-1 (MEOX1) . P50221
Other
Click To Hide/Show 6 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Gamma-aminobutyric acid receptor-associated protein-like 1 (GABARAPL1) ATG8 family Q9H0R8
Gamma-aminobutyric acid receptor-associated protein-like 2 (GABARAPL2) ATG8 family P60520
DNA damage-inducible transcript 4-like protein (DDIT4L) DDIT4 family Q96D03
Meiosis-specific protein MEI4 (MEI4) MEI4L family A8MW99
Keratin-associated protein 13-3 (KRTAP13-3) PMG family Q3SY46
TLE family member 5 (TLE5) WD repeat Groucho/TLE family Q08117

References

1 A Chemoproteomic Strategy for Direct and Proteome-Wide Covalent Inhibitor Target-Site Identification. J Am Chem Soc. 2019 Jan 9;141(1):191-203. doi: 10.1021/jacs.8b07911. Epub 2018 Dec 20.
2 An Activity-Guided Map of Electrophile-Cysteine Interactions in Primary Human T Cells. Cell. 2020 Aug 20;182(4):1009-1026.e29. doi: 10.1016/j.cell.2020.07.001. Epub 2020 Jul 29.
3 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
4 Benchmarking Cleavable Biotin Tags for Peptide-Centric Chemoproteomics. J Proteome Res. 2022 May 6;21(5):1349-1358. doi: 10.1021/acs.jproteome.2c00174. Epub 2022 Apr 25.
Mass spectrometry data entry: PXD031019
5 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
6 DFT-Guided Discovery of Ethynyl-Triazolyl-Phosphinates as Modular Electrophiles for Chemoselective Cysteine Bioconjugation and Profiling. Angew Chem Int Ed Engl. 2022 Oct 10;61(41):e202205348. doi: 10.1002/anie.202205348. Epub 2022 Aug 22.
Mass spectrometry data entry: PXD033004
7 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.
8 Site-specific quantitative cysteine profiling with data-independent acquisition-based mass spectrometry. Methods Enzymol. 2023;679:295-322. doi: 10.1016/bs.mie.2022.07.037. Epub 2022 Sep 7.
Mass spectrometry data entry: PXD027578
9 Rapid Covalent-Probe Discovery by Electrophile-Fragment Screening. J Am Chem Soc. 2019 Jun 5;141(22):8951-8968. doi: 10.1021/jacs.9b02822. Epub 2019 May 22.