Details of the Target

General Information of Target

Target ID LDTP12955
Target Name Mediator of RNA polymerase II transcription subunit 11 (MED11)
Gene Name MED11
Gene ID 400569
Synonyms
Mediator of RNA polymerase II transcription subunit 11; Mediator complex subunit 11
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MENQVLTPHVYWAQRHRELYLRVELSDVQNPAISITENVLHFKAQGHGAKGDNVYEFHLE
FLDLVKPEPVYKLTQRQVNITVQKKVSQWWERLTKQEKRPLFLAPDFDRWLDESDAEMEL
RAKEEERLNKLRLESEGSPETLTNLRKGYLFMYNLVQFLGFSWIFVNLTVRFCILGKESF
YDTFHTVADMMYFCQMLAVVETINAAIGVTTSPVLPSLIQLLGRNFILFIIFGTMEEMQN
KAVVFFVFYLWSAIEIFRYSFYMLTCIDMDWKVLTWLRYTLWIPLYPLGCLAEAVSVIQS
IPIFNETGRFSFTLPYPVKIKVRFSFFLQIYLIMIFLGLYINFRHLYKQRRRRYGQKKKK
IH
Target Bioclass
Other
Family
Mediator complex subunit 11 family
Subcellular location
Nucleus
Function
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for theee assembly of a functional pre-initiation complex with RNA polymerase II and the general transcription factors.
Uniprot ID
Q9P086
Ensemble ID
ENST00000293777.6
HGNC ID
HGNC:32687

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 8 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
STPyne
 Probe Info  		K103(10.00); K95(1.76)  LDD0277  [1]
BTD
 Probe Info  		C90(0.70)  LDD2097  [2]
DBIA
 Probe Info  		C90(1.09)  LDD1509  [3]
m-APA
 Probe Info  		N.A.  LDD2232  [4]
IA-alkyne
 Probe Info  		N.A.  LDD0165  [5]
Lodoacetamide azide
 Probe Info  		N.A.  LDD0037  [6]
IPM
 Probe Info  		N.A.  LDD2156  [7]
AOyne
 Probe Info  		15.00  LDD0443  [8]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0519  1-(6-methoxy-3,4-dihydroquinolin-1(2H)-yl)-2-nitroethan-1-one MDA-MB-231 C90(0.75)  LDD2112  [2]
 LDCM0226  AC11 HEK-293T C90(1.09)  LDD1509  [3]
 LDCM0278  AC19 HEK-293T C90(1.08)  LDD1517  [3]
 LDCM0284  AC24 HEK-293T C90(1.01)  LDD1523  [3]
 LDCM0287  AC27 HEK-293T C90(1.10)  LDD1526  [3]
 LDCM0290  AC3 HEK-293T C90(1.03)  LDD1529  [3]
 LDCM0293  AC32 HEK-293T C90(1.00)  LDD1532  [3]
 LDCM0296  AC35 HEK-293T C90(1.07)  LDD1535  [3]
 LDCM0302  AC40 HEK-293T C90(1.02)  LDD1541  [3]
 LDCM0305  AC43 HEK-293T C90(1.15)  LDD1544  [3]
 LDCM0310  AC48 HEK-293T C90(1.03)  LDD1549  [3]
 LDCM0314  AC51 HEK-293T C90(1.10)  LDD1553  [3]
 LDCM0319  AC56 HEK-293T C90(0.96)  LDD1558  [3]
 LDCM0322  AC59 HEK-293T C90(1.13)  LDD1561  [3]
 LDCM0328  AC64 HEK-293T C90(1.04)  LDD1567  [3]
 LDCM0345  AC8 HEK-293T C90(0.96)  LDD1569  [3]
 LDCM0275  AKOS034007705 HEK-293T C90(1.03)  LDD1514  [3]
 LDCM0369  CL100 HEK-293T C90(1.05)  LDD1573  [3]
 LDCM0373  CL104 HEK-293T C90(1.04)  LDD1577  [3]
 LDCM0377  CL108 HEK-293T C90(1.04)  LDD1581  [3]
 LDCM0382  CL112 HEK-293T C90(1.04)  LDD1586  [3]
 LDCM0386  CL116 HEK-293T C90(1.06)  LDD1590  [3]
 LDCM0390  CL12 HEK-293T C90(1.15)  LDD1594  [3]
 LDCM0391  CL120 HEK-293T C90(1.04)  LDD1595  [3]
 LDCM0395  CL124 HEK-293T C90(1.03)  LDD1599  [3]
 LDCM0399  CL128 HEK-293T C90(1.00)  LDD1603  [3]
 LDCM0403  CL16 HEK-293T C90(1.08)  LDD1607  [3]
 LDCM0406  CL19 HEK-293T C90(1.10)  LDD1610  [3]
 LDCM0412  CL24 HEK-293T C90(1.22)  LDD1616  [3]
 LDCM0416  CL28 HEK-293T C90(1.15)  LDD1620  [3]
 LDCM0420  CL31 HEK-293T C90(1.14)  LDD1624  [3]
 LDCM0425  CL36 HEK-293T C90(1.21)  LDD1629  [3]
 LDCM0429  CL4 HEK-293T C90(1.15)  LDD1633  [3]
 LDCM0430  CL40 HEK-293T C90(1.11)  LDD1634  [3]
 LDCM0433  CL43 HEK-293T C90(1.11)  LDD1637  [3]
 LDCM0438  CL48 HEK-293T C90(1.17)  LDD1642  [3]
 LDCM0443  CL52 HEK-293T C90(1.04)  LDD1646  [3]
 LDCM0446  CL55 HEK-293T C90(1.16)  LDD1649  [3]
 LDCM0452  CL60 HEK-293T C90(1.24)  LDD1655  [3]
 LDCM0456  CL64 HEK-293T C90(1.20)  LDD1659  [3]
 LDCM0459  CL67 HEK-293T C90(1.13)  LDD1662  [3]
 LDCM0462  CL7 HEK-293T C90(1.08)  LDD1665  [3]
 LDCM0465  CL72 HEK-293T C90(1.14)  LDD1668  [3]
 LDCM0469  CL76 HEK-293T C90(1.05)  LDD1672  [3]
 LDCM0472  CL79 HEK-293T C90(1.07)  LDD1675  [3]
 LDCM0478  CL84 HEK-293T C90(1.14)  LDD1681  [3]
 LDCM0482  CL88 HEK-293T C90(1.13)  LDD1685  [3]
 LDCM0486  CL91 HEK-293T C90(1.08)  LDD1689  [3]
 LDCM0491  CL96 HEK-293T C90(1.21)  LDD1694  [3]
 LDCM0504  Nucleophilic fragment 15a MDA-MB-231 C90(0.70)  LDD2097  [2]
 LDCM0535  Nucleophilic fragment 30b MDA-MB-231 C90(0.92)  LDD2128  [2]
 LDCM0547  Nucleophilic fragment 41 MDA-MB-231 C90(0.68)  LDD2141  [2]
 LDCM0551  Nucleophilic fragment 5b MDA-MB-231 C90(0.30)  LDD2145  [2]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Nucleoside diphosphate kinase homolog 7 (NME7) NDK family Q9Y5B8
Soluble scavenger receptor cysteine-rich domain-containing protein SSC5D (SSC5D) . A1L4H1
Other
Click To Hide/Show 7 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Glycine cleavage system H protein, mitochondrial (GCSH) GcvH family P23434
Harmonin-binding protein USHBP1 (USHBP1) MCC family Q8N6Y0
Mediator of RNA polymerase II transcription subunit 22 (MED22) Mediator complex subunit 22 family Q15528
Nuclear distribution protein nudE-like 1 (NDEL1) NudE family Q9GZM8
SAGA-associated factor 29 (SGF29) SGF29 family Q96ES7
Synaptonemal complex central element protein 2 (SYCE2) SYCE family Q6PIF2
Coiled-coil domain-containing protein 120 (CCDC120) . Q96HB5

References

1 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
2 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
3 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
4 Global profiling of functional histidines in live cells using small-molecule photosensitizer and chemical probe relay labelling. Nat Chem. 2024 Jun 4. doi: 10.1038/s41557-024-01545-6. Online ahead of print.
Mass spectrometry data entry: PXD042377
5 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
6 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
7 Benchmarking Cleavable Biotin Tags for Peptide-Centric Chemoproteomics. J Proteome Res. 2022 May 6;21(5):1349-1358. doi: 10.1021/acs.jproteome.2c00174. Epub 2022 Apr 25.
Mass spectrometry data entry: PXD031019
8 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.