Details of the Target

General Information of Target

Target ID LDTP12220
Target Name Centromere protein J (CENPJ)
Gene Name CENPJ
Gene ID 55835
Synonyms
CPAP; LAP; LIP1; Centromere protein J; CENP-J; Centrosomal P4.1-associated protein; LAG-3-associated protein; LYST-interacting protein 1
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MGRLVLLWGAAVFLLGGWMALGQGGAAEGVQIQIIYFNLETVQVTWNASKYSRTNLTFHY
RFNGDEAYDQCTNYLLQEGHTSGCLLDAEQRDDILYFSIRNGTHPVFTASRWMVYYLKPS
SPKHVRFSWHQDAVTVTCSDLSYGDLLYEVQYRSPFDTEWQSKQENTCNVTIEGLDAEKC
YSFWVRVKAMEDVYGPDTYPSDWSEVTCWQRGEIRDACAETPTPPKPKLSKFILISSLAI
LLMVSLLLLSLWKLWRVKKFLIPSVPDPKSIFPGLFEIHQGNFQEWITDTQNVAHLHKMA
GAEQESGPEEPLVVQLAKTEAESPRMLDPQTEEKEASGGSLQLPHQPLQGGDVVTIGGFT
FVMNDRSYVAL
Target Bioclass
Other
Family
TCP10 family
Subcellular location
Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
Function
Plays an important role in cell division and centrosome function by participating in centriole duplication. Inhibits microtubule nucleation from the centrosome. Involved in the regulation of slow processive growth of centriolar microtubules. Acts as a microtubule plus-end tracking protein that stabilizes centriolar microtubules and inhibits microtubule polymerization and extension from the distal ends of centrioles. Required for centriole elongation and for STIL-mediated centriole amplification. Required for the recruitment of CEP295 to the proximal end of new-born centrioles at the centriolar microtubule wall during early S phase in a PLK4-dependent manner. May be involved in the control of centriolar-microtubule growth by acting as a regulator of tubulin release.
Uniprot ID
Q9HC77
Ensemble ID
ENST00000381884.9
HGNC ID
HGNC:17272

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
AN3CA SNV: p.S1022N DBIA    Probe Info 
CAL27 SNV: p.E324Q; p.D720A .
CMK SNV: p.S632C DBIA    Probe Info 
IGROV1 SNV: p.G724S .
MDAMB231 SNV: p.S1075F .
MEWO SNV: p.P1138S DBIA    Probe Info 
MFE319 Deletion: p.N277IfsTer3 DBIA    Probe Info 
NALM6 SNV: p.A425T .
NCIH1703 SNV: p.L661F DBIA    Probe Info 
PFSK1 Deletion: p.E1034TfsTer8 DBIA    Probe Info 
SNGM SNV: p.T915I .
TGBC52TKB SNV: p.Y232Ter .
VCAP SNV: p.P1104T .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 5 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C506(1.01); C510(1.01); C514(1.01)  LDD0078  [1]
CY-1
 Probe Info  		N.A.  LDD0246  [2]
4-Iodoacetamidophenylacetylene
 Probe Info  		N.A.  LDD0038  [3]
IPM
 Probe Info  		N.A.  LDD2156  [4]
Phosphinate-6
 Probe Info  		N.A.  LDD0018  [5]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0259  AC14 HEK-293T C432(1.03)  LDD1512  [6]
 LDCM0270  AC15 HEK-293T C432(1.08)  LDD1513  [6]
 LDCM0281  AC21 HEK-293T C634(0.92)  LDD1520  [6]
 LDCM0282  AC22 HEK-293T C432(1.03)  LDD1521  [6]
 LDCM0283  AC23 HEK-293T C432(0.94)  LDD1522  [6]
 LDCM0284  AC24 HEK-293T C432(1.16)  LDD1523  [6]
 LDCM0289  AC29 HEK-293T C634(1.12)  LDD1528  [6]
 LDCM0291  AC30 HEK-293T C432(1.04)  LDD1530  [6]
 LDCM0292  AC31 HEK-293T C432(1.17)  LDD1531  [6]
 LDCM0293  AC32 HEK-293T C432(1.05)  LDD1532  [6]
 LDCM0298  AC37 HEK-293T C634(1.14)  LDD1537  [6]
 LDCM0299  AC38 HEK-293T C432(1.02)  LDD1538  [6]
 LDCM0300  AC39 HEK-293T C432(1.05)  LDD1539  [6]
 LDCM0302  AC40 HEK-293T C432(1.29)  LDD1541  [6]
 LDCM0307  AC45 HEK-293T C634(1.11)  LDD1546  [6]
 LDCM0308  AC46 HEK-293T C432(1.01)  LDD1547  [6]
 LDCM0309  AC47 HEK-293T C432(0.94)  LDD1548  [6]
 LDCM0310  AC48 HEK-293T C432(1.02)  LDD1549  [6]
 LDCM0312  AC5 HEK-293T C634(0.97)  LDD1551  [6]
 LDCM0316  AC53 HEK-293T C634(1.04)  LDD1555  [6]
 LDCM0317  AC54 HEK-293T C432(1.18)  LDD1556  [6]
 LDCM0318  AC55 HEK-293T C432(0.99)  LDD1557  [6]
 LDCM0319  AC56 HEK-293T C432(1.39)  LDD1558  [6]
 LDCM0323  AC6 HEK-293T C432(1.16)  LDD1562  [6]
 LDCM0325  AC61 HEK-293T C634(0.98)  LDD1564  [6]
 LDCM0326  AC62 HEK-293T C432(1.05)  LDD1565  [6]
 LDCM0327  AC63 HEK-293T C432(0.98)  LDD1566  [6]
 LDCM0328  AC64 HEK-293T C432(1.00)  LDD1567  [6]
 LDCM0334  AC7 HEK-293T C432(1.23)  LDD1568  [6]
 LDCM0345  AC8 HEK-293T C432(1.08)  LDD1569  [6]
 LDCM0248  AKOS034007472 HEK-293T C634(1.02)  LDD1511  [6]
 LDCM0275  AKOS034007705 HEK-293T C432(1.15)  LDD1514  [6]
 LDCM0020  ARS-1620 HCC44 C506(1.01); C510(1.01); C514(1.01)  LDD0078  [1]
 LDCM0367  CL1 HEK-293T C432(0.99)  LDD1571  [6]
 LDCM0368  CL10 HEK-293T C432(1.12)  LDD1572  [6]
 LDCM0369  CL100 HEK-293T C432(0.85)  LDD1573  [6]
 LDCM0370  CL101 HEK-293T C432(1.12)  LDD1574  [6]
 LDCM0372  CL103 HEK-293T C506(0.90)  LDD1576  [6]
 LDCM0373  CL104 HEK-293T C432(0.98)  LDD1577  [6]
 LDCM0374  CL105 HEK-293T C432(1.00)  LDD1578  [6]
 LDCM0376  CL107 HEK-293T C506(1.03)  LDD1580  [6]
 LDCM0377  CL108 HEK-293T C432(0.92)  LDD1581  [6]
 LDCM0378  CL109 HEK-293T C432(1.01)  LDD1582  [6]
 LDCM0379  CL11 HEK-293T C432(1.17)  LDD1583  [6]
 LDCM0381  CL111 HEK-293T C506(0.87)  LDD1585  [6]
 LDCM0382  CL112 HEK-293T C432(0.91)  LDD1586  [6]
 LDCM0383  CL113 HEK-293T C432(1.19)  LDD1587  [6]
 LDCM0385  CL115 HEK-293T C506(0.90)  LDD1589  [6]
 LDCM0386  CL116 HEK-293T C432(0.96)  LDD1590  [6]
 LDCM0387  CL117 HEK-293T C432(0.98)  LDD1591  [6]
 LDCM0389  CL119 HEK-293T C506(1.01)  LDD1593  [6]
 LDCM0390  CL12 HEK-293T C432(0.89)  LDD1594  [6]
 LDCM0391  CL120 HEK-293T C432(0.97)  LDD1595  [6]
 LDCM0392  CL121 HEK-293T C432(1.09)  LDD1596  [6]
 LDCM0394  CL123 HEK-293T C506(0.88)  LDD1598  [6]
 LDCM0395  CL124 HEK-293T C432(0.99)  LDD1599  [6]
 LDCM0396  CL125 HEK-293T C432(1.11)  LDD1600  [6]
 LDCM0398  CL127 HEK-293T C506(0.89)  LDD1602  [6]
 LDCM0399  CL128 HEK-293T C432(0.86)  LDD1603  [6]
 LDCM0400  CL13 HEK-293T C432(1.18)  LDD1604  [6]
 LDCM0402  CL15 HEK-293T C506(0.91)  LDD1606  [6]
 LDCM0403  CL16 HEK-293T C432(0.95)  LDD1607  [6]
 LDCM0409  CL21 HEK-293T C634(1.08)  LDD1613  [6]
 LDCM0410  CL22 HEK-293T C432(1.34)  LDD1614  [6]
 LDCM0411  CL23 HEK-293T C432(1.17)  LDD1615  [6]
 LDCM0412  CL24 HEK-293T C432(1.23)  LDD1616  [6]
 LDCM0413  CL25 HEK-293T C432(0.91)  LDD1617  [6]
 LDCM0415  CL27 HEK-293T C506(1.02)  LDD1619  [6]
 LDCM0416  CL28 HEK-293T C432(0.99)  LDD1620  [6]
 LDCM0418  CL3 HEK-293T C506(0.86)  LDD1622  [6]
 LDCM0422  CL33 HEK-293T C634(1.05)  LDD1626  [6]
 LDCM0423  CL34 HEK-293T C432(1.11)  LDD1627  [6]
 LDCM0424  CL35 HEK-293T C432(1.03)  LDD1628  [6]
 LDCM0425  CL36 HEK-293T C432(0.95)  LDD1629  [6]
 LDCM0426  CL37 HEK-293T C432(0.92)  LDD1630  [6]
 LDCM0428  CL39 HEK-293T C506(0.82)  LDD1632  [6]
 LDCM0429  CL4 HEK-293T C432(0.97)  LDD1633  [6]
 LDCM0430  CL40 HEK-293T C432(0.96)  LDD1634  [6]
 LDCM0435  CL45 HEK-293T C634(1.16)  LDD1639  [6]
 LDCM0436  CL46 HEK-293T C432(1.14)  LDD1640  [6]
 LDCM0437  CL47 HEK-293T C432(1.12)  LDD1641  [6]
 LDCM0438  CL48 HEK-293T C432(1.03)  LDD1642  [6]
 LDCM0439  CL49 HEK-293T C432(0.96)  LDD1643  [6]
 LDCM0443  CL52 HEK-293T C432(0.99)  LDD1646  [6]
 LDCM0448  CL57 HEK-293T C634(1.14)  LDD1651  [6]
 LDCM0449  CL58 HEK-293T C432(1.13)  LDD1652  [6]
 LDCM0450  CL59 HEK-293T C432(1.11)  LDD1653  [6]
 LDCM0452  CL60 HEK-293T C432(1.16)  LDD1655  [6]
 LDCM0453  CL61 HEK-293T C432(0.97)  LDD1656  [6]
 LDCM0455  CL63 HEK-293T C506(0.92)  LDD1658  [6]
 LDCM0456  CL64 HEK-293T C432(0.98)  LDD1659  [6]
 LDCM0461  CL69 HEK-293T C634(1.15)  LDD1664  [6]
 LDCM0463  CL70 HEK-293T C432(1.05)  LDD1666  [6]
 LDCM0464  CL71 HEK-293T C432(1.03)  LDD1667  [6]
 LDCM0465  CL72 HEK-293T C432(0.99)  LDD1668  [6]
 LDCM0466  CL73 HEK-293T C432(0.88)  LDD1669  [6]
 LDCM0469  CL76 HEK-293T C432(1.03)  LDD1672  [6]
 LDCM0475  CL81 HEK-293T C634(1.24)  LDD1678  [6]
 LDCM0476  CL82 HEK-293T C432(1.09)  LDD1679  [6]
 LDCM0477  CL83 HEK-293T C432(1.05)  LDD1680  [6]
 LDCM0478  CL84 HEK-293T C432(1.00)  LDD1681  [6]
 LDCM0479  CL85 HEK-293T C432(0.97)  LDD1682  [6]
 LDCM0481  CL87 HEK-293T C506(0.91)  LDD1684  [6]
 LDCM0482  CL88 HEK-293T C432(1.01)  LDD1685  [6]
 LDCM0484  CL9 HEK-293T C634(1.17)  LDD1687  [6]
 LDCM0488  CL93 HEK-293T C634(1.11)  LDD1691  [6]
 LDCM0489  CL94 HEK-293T C432(1.32)  LDD1692  [6]
 LDCM0490  CL95 HEK-293T C432(0.92)  LDD1693  [6]
 LDCM0491  CL96 HEK-293T C432(0.92)  LDD1694  [6]
 LDCM0492  CL97 HEK-293T C432(1.08)  LDD1695  [6]
 LDCM0494  CL99 HEK-293T C506(0.75)  LDD1697  [6]
 LDCM0495  E2913 HEK-293T C506(1.36)  LDD1698  [6]
 LDCM0468  Fragment33 HEK-293T C506(0.79)  LDD1671  [6]
 LDCM0022  KB02 697 C432(1.77)  LDD2245  [7]
 LDCM0023  KB03 Jurkat C634(19.09); C608(45.20)  LDD0209  [8]
 LDCM0024  KB05 UACC257 C634(1.64)  LDD3325  [7]
 LDCM0021  THZ1 HCT 116 C216(0.96); C217(0.96); C245(0.96); C510(0.92)  LDD2173  [1]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Poly [ADP-ribose] polymerase tankyrase-1 (TNKS) ARTD/PARP family O95271
Serine/threonine-protein kinase PLK2 (PLK2) Ser/Thr protein kinase family Q9NYY3
Transporter and channel
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
14-3-3 protein gamma (YWHAG) 14-3-3 family P61981
Transcription factor
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Protein capicua homolog (CIC) . Q96RK0
Zinc finger protein Gfi-1b (GFI1B) . Q5VTD9
Other
Click To Hide/Show 4 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
BRCA2 and CDKN1A-interacting protein (BCCIP) BCP1 family Q9P287
Centrosomal protein of 135 kDa (CEP135) CEP135/TSGA10 family Q66GS9
Centromere protein J (CENPJ) TCP10 family Q9HC77
SCL-interrupting locus protein (STIL) . Q15468

References

1 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
2 Cyclopropenone, Cyclopropeniminium Ion, and Cyclopropenethione as Novel Electrophilic Warheads for Potential Target Discovery of Triple-Negative Breast Cancer. J Med Chem. 2023 Feb 23;66(4):2851-2864. doi: 10.1021/acs.jmedchem.2c01889. Epub 2023 Feb 10.
3 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
4 Benchmarking Cleavable Biotin Tags for Peptide-Centric Chemoproteomics. J Proteome Res. 2022 May 6;21(5):1349-1358. doi: 10.1021/acs.jproteome.2c00174. Epub 2022 Apr 25.
Mass spectrometry data entry: PXD031019
5 DFT-Guided Discovery of Ethynyl-Triazolyl-Phosphinates as Modular Electrophiles for Chemoselective Cysteine Bioconjugation and Profiling. Angew Chem Int Ed Engl. 2022 Oct 10;61(41):e202205348. doi: 10.1002/anie.202205348. Epub 2022 Aug 22.
Mass spectrometry data entry: PXD033004
6 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
7 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
8 Covalent Inhibition by a Natural Product-Inspired Latent Electrophile. J Am Chem Soc. 2023 May 24;145(20):11097-11109. doi: 10.1021/jacs.3c00598. Epub 2023 May 15.