Details of the Target

General Information of Target

Target ID LDTP12076
Target Name Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase 2, mitochondrial (MTHFD2L)
Gene Name MTHFD2L
Gene ID 441024
Synonyms
Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase 2, mitochondrial; NADP-dependent methylenetetrahydrofolate dehydrogenase 2-like protein; MTHFD2-like) [Includes: NAD-dependent methylenetetrahydrofolate dehydrogenase; EC 1.5.1.15; EC 1.5.1.5; Methenyltetrahydrofolate cyclohydrolase; EC 3.5.4.9)]
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MVSWIISRLVVLIFGTLYPAYYSYKAVKSKDIKEYVKWMMYWIIFALFTTAETFTDIFLC
WFPFYYELKIAFVAWLLSPYTKGSSLLYRKFVHPTLSSKEKEIDDCLVQAKDRSYDALVH
FGKRGLNVAATAAVMAASKGQGALSERLRSFSMQDLTTIRGDGAPAPSGPPPPGSGRASG
KHGQPKMSRSASESASSSGTA
Target Bioclass
Enzyme
Family
Tetrahydrofolate dehydrogenase/cyclohydrolase family
Subcellular location
Mitochondrion inner membrane
Function Bifunctional mitochondrial folate-interconverting enzyme that has both NAD/NADP-dependent methylenetetrahydrofolate dehydrogenase and methenyltetrahydrofolate cyclohydrolase activities.
Uniprot ID
Q9H903
Ensemble ID
ENST00000325278.7
HGNC ID
HGNC:31865
ChEMBL ID
CHEMBL4105963

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 3 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
TH216
 Probe Info  		Y248(12.22)  LDD0259  [1]
DBIA
 Probe Info  		C180(1.04)  LDD1508  [2]
Phosphinate-6
 Probe Info  		N.A.  LDD0018  [3]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0215  AC10 HEK-293T C180(1.04)  LDD1508  [2]
 LDCM0237  AC12 HEK-293T C112(1.27); C180(0.92)  LDD1510  [2]
 LDCM0259  AC14 HEK-293T C180(1.04)  LDD1512  [2]
 LDCM0270  AC15 HEK-293T C112(1.25); C180(0.98)  LDD1513  [2]
 LDCM0277  AC18 HEK-293T C180(0.97)  LDD1516  [2]
 LDCM0279  AC2 HEK-293T C180(0.99)  LDD1518  [2]
 LDCM0280  AC20 HEK-293T C112(1.01); C180(0.86)  LDD1519  [2]
 LDCM0281  AC21 HEK-293T C112(1.09); C180(1.19)  LDD1520  [2]
 LDCM0282  AC22 HEK-293T C180(1.05)  LDD1521  [2]
 LDCM0283  AC23 HEK-293T C112(1.13); C180(1.00)  LDD1522  [2]
 LDCM0284  AC24 HEK-293T C180(0.90)  LDD1523  [2]
 LDCM0286  AC26 HEK-293T C180(0.93)  LDD1525  [2]
 LDCM0288  AC28 HEK-293T C112(1.02); C180(0.93)  LDD1527  [2]
 LDCM0289  AC29 HEK-293T C112(1.23); C180(1.02)  LDD1528  [2]
 LDCM0291  AC30 HEK-293T C180(0.95)  LDD1530  [2]
 LDCM0292  AC31 HEK-293T C112(1.10); C180(1.22)  LDD1531  [2]
 LDCM0293  AC32 HEK-293T C180(0.92)  LDD1532  [2]
 LDCM0295  AC34 HEK-293T C180(0.88)  LDD1534  [2]
 LDCM0297  AC36 HEK-293T C112(1.13); C180(1.04)  LDD1536  [2]
 LDCM0298  AC37 HEK-293T C112(1.15); C180(0.99)  LDD1537  [2]
 LDCM0299  AC38 HEK-293T C180(1.06)  LDD1538  [2]
 LDCM0300  AC39 HEK-293T C112(1.09); C180(1.04)  LDD1539  [2]
 LDCM0301  AC4 HEK-293T C112(1.04); C180(0.83)  LDD1540  [2]
 LDCM0302  AC40 HEK-293T C180(1.04)  LDD1541  [2]
 LDCM0304  AC42 HEK-293T C180(1.03)  LDD1543  [2]
 LDCM0306  AC44 HEK-293T C112(1.18); C180(1.01)  LDD1545  [2]
 LDCM0307  AC45 HEK-293T C112(1.09); C180(1.09)  LDD1546  [2]
 LDCM0308  AC46 HEK-293T C180(1.15)  LDD1547  [2]
 LDCM0309  AC47 HEK-293T C112(1.15); C180(1.08)  LDD1548  [2]
 LDCM0310  AC48 HEK-293T C180(0.91)  LDD1549  [2]
 LDCM0312  AC5 HEK-293T C112(1.12); C180(1.09)  LDD1551  [2]
 LDCM0313  AC50 HEK-293T C180(0.97)  LDD1552  [2]
 LDCM0315  AC52 HEK-293T C112(0.98); C180(1.07)  LDD1554  [2]
 LDCM0316  AC53 HEK-293T C112(1.08); C180(1.03)  LDD1555  [2]
 LDCM0317  AC54 HEK-293T C180(1.04)  LDD1556  [2]
 LDCM0318  AC55 HEK-293T C112(1.05); C180(1.04)  LDD1557  [2]
 LDCM0319  AC56 HEK-293T C180(1.07)  LDD1558  [2]
 LDCM0321  AC58 HEK-293T C180(0.98)  LDD1560  [2]
 LDCM0323  AC6 HEK-293T C180(1.06)  LDD1562  [2]
 LDCM0324  AC60 HEK-293T C112(1.15); C180(1.00)  LDD1563  [2]
 LDCM0325  AC61 HEK-293T C112(1.27); C180(1.06)  LDD1564  [2]
 LDCM0326  AC62 HEK-293T C180(1.07)  LDD1565  [2]
 LDCM0327  AC63 HEK-293T C112(1.15); C180(1.09)  LDD1566  [2]
 LDCM0328  AC64 HEK-293T C180(1.15)  LDD1567  [2]
 LDCM0334  AC7 HEK-293T C112(1.11); C180(1.06)  LDD1568  [2]
 LDCM0345  AC8 HEK-293T C180(1.05)  LDD1569  [2]
 LDCM0248  AKOS034007472 HEK-293T C112(1.04); C180(1.01)  LDD1511  [2]
 LDCM0275  AKOS034007705 HEK-293T C180(0.97)  LDD1514  [2]
 LDCM0368  CL10 HEK-293T C180(1.02)  LDD1572  [2]
 LDCM0379  CL11 HEK-293T C112(0.84); C180(1.03)  LDD1583  [2]
 LDCM0390  CL12 HEK-293T C180(0.97)  LDD1594  [2]
 LDCM0405  CL18 HEK-293T C180(0.95)  LDD1609  [2]
 LDCM0408  CL20 HEK-293T C112(0.80); C180(0.87)  LDD1612  [2]
 LDCM0409  CL21 HEK-293T C112(0.80); C180(0.98)  LDD1613  [2]
 LDCM0410  CL22 HEK-293T C180(0.94)  LDD1614  [2]
 LDCM0411  CL23 HEK-293T C112(0.84); C180(1.19)  LDD1615  [2]
 LDCM0412  CL24 HEK-293T C180(0.96)  LDD1616  [2]
 LDCM0419  CL30 HEK-293T C180(1.05)  LDD1623  [2]
 LDCM0421  CL32 HEK-293T C112(0.77); C180(0.80)  LDD1625  [2]
 LDCM0422  CL33 HEK-293T C112(0.76); C180(0.89)  LDD1626  [2]
 LDCM0423  CL34 HEK-293T C180(0.94)  LDD1627  [2]
 LDCM0424  CL35 HEK-293T C112(0.75); C180(0.94)  LDD1628  [2]
 LDCM0425  CL36 HEK-293T C180(0.98)  LDD1629  [2]
 LDCM0432  CL42 HEK-293T C180(0.86)  LDD1636  [2]
 LDCM0434  CL44 HEK-293T C112(0.71); C180(1.00)  LDD1638  [2]
 LDCM0435  CL45 HEK-293T C112(0.82); C180(0.93)  LDD1639  [2]
 LDCM0436  CL46 HEK-293T C180(0.91)  LDD1640  [2]
 LDCM0437  CL47 HEK-293T C112(0.85); C180(0.94)  LDD1641  [2]
 LDCM0438  CL48 HEK-293T C180(0.92)  LDD1642  [2]
 LDCM0445  CL54 HEK-293T C180(0.80)  LDD1648  [2]
 LDCM0447  CL56 HEK-293T C112(0.87); C180(1.00)  LDD1650  [2]
 LDCM0448  CL57 HEK-293T C112(0.71); C180(1.11)  LDD1651  [2]
 LDCM0449  CL58 HEK-293T C180(1.18)  LDD1652  [2]
 LDCM0450  CL59 HEK-293T C112(0.80); C180(1.14)  LDD1653  [2]
 LDCM0451  CL6 HEK-293T C180(0.81)  LDD1654  [2]
 LDCM0452  CL60 HEK-293T C180(1.12)  LDD1655  [2]
 LDCM0458  CL66 HEK-293T C180(1.01)  LDD1661  [2]
 LDCM0460  CL68 HEK-293T C112(0.75); C180(0.88)  LDD1663  [2]
 LDCM0461  CL69 HEK-293T C112(0.71); C180(1.13)  LDD1664  [2]
 LDCM0463  CL70 HEK-293T C180(0.96)  LDD1666  [2]
 LDCM0464  CL71 HEK-293T C112(0.83); C180(0.93)  LDD1667  [2]
 LDCM0465  CL72 HEK-293T C180(0.88)  LDD1668  [2]
 LDCM0471  CL78 HEK-293T C180(1.01)  LDD1674  [2]
 LDCM0473  CL8 HEK-293T C112(0.76); C180(0.84)  LDD1676  [2]
 LDCM0474  CL80 HEK-293T C112(0.82); C180(0.86)  LDD1677  [2]
 LDCM0475  CL81 HEK-293T C112(0.79); C180(0.94)  LDD1678  [2]
 LDCM0476  CL82 HEK-293T C180(1.03)  LDD1679  [2]
 LDCM0477  CL83 HEK-293T C112(0.81); C180(0.98)  LDD1680  [2]
 LDCM0478  CL84 HEK-293T C180(1.00)  LDD1681  [2]
 LDCM0484  CL9 HEK-293T C112(0.75); C180(1.07)  LDD1687  [2]
 LDCM0485  CL90 HEK-293T C180(0.82)  LDD1688  [2]
 LDCM0487  CL92 HEK-293T C112(0.92); C180(0.91)  LDD1690  [2]
 LDCM0488  CL93 HEK-293T C112(0.82); C180(1.11)  LDD1691  [2]
 LDCM0489  CL94 HEK-293T C180(1.15)  LDD1692  [2]
 LDCM0490  CL95 HEK-293T C112(0.85); C180(0.82)  LDD1693  [2]
 LDCM0491  CL96 HEK-293T C180(0.93)  LDD1694  [2]
 LDCM0022  KB02 MGG123 C159(1.97)  LDD2469  [4]
 LDCM0023  KB03 MGG123 C159(2.08)  LDD2886  [4]
 LDCM0024  KB05 MGG123 C159(1.91)  LDD3303  [4]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Protein N-terminal glutamine amidohydrolase (NTAQ1) NTAQ1 family Q96HA8

References

1 Chemoproteomic profiling of kinases in live cells using electrophilic sulfonyl triazole probes. Chem Sci. 2021 Jan 21;12(9):3295-3307. doi: 10.1039/d0sc06623k.
2 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
3 DFT-Guided Discovery of Ethynyl-Triazolyl-Phosphinates as Modular Electrophiles for Chemoselective Cysteine Bioconjugation and Profiling. Angew Chem Int Ed Engl. 2022 Oct 10;61(41):e202205348. doi: 10.1002/anie.202205348. Epub 2022 Aug 22.
Mass spectrometry data entry: PXD033004
4 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840