Details of the Target

General Information of Target

Target ID LDTP12015
Target Name SH2 domain-containing protein 4A (SH2D4A)
Gene Name SH2D4A
Gene ID 63898
Synonyms
PPP1R38; SH2A; SH2 domain-containing protein 4A; Protein SH(2)A; Protein phosphatase 1 regulatory subunit 38
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MSMDVTFLGTGAAYPSPTRGASAVVLRCEGECWLFDCGEGTQTQLMKSQLKAGRITKIFI
THLHGDHFFGLPGLLCTISLQSGSMVSKQPIEIYGPVGLRDFIWRTMELSHTELVFHYVV
HELVPTADQCPAEELKEFAHVNRADSPPKEEQGRTILLDSEENSYLLFDDEQFVVKAFRL
FHRIPSFGFSVVEKKRPGKLNAQKLKDLGVPPGPAYGKLKNGISVVLENGVTISPQDVLK
KPIVGRKICILGDCSGVVGDGGVKLCFEADLLIHEATLDDAQMDKAKEHGHSTPQMAATF
AKLCRAKRLVLTHFSQRYKPVALAREGETDGIAELKKQAESVLDLQEVTLAEDFMVISIP
IKK
Target Bioclass
Other
Subcellular location
Cytoplasm
Function
Inhibits estrogen-induced cell proliferation by competing with PLCG for binding to ESR1, blocking the effect of estrogen on PLCG and repressing estrogen-induced proliferation. May play a role in T-cell development and function.
Uniprot ID
Q9H788
Ensemble ID
ENST00000265807.8
HGNC ID
HGNC:26102

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
CAL72 SNV: p.M46V .
HCT15 SNV: p.L52M .
HEC1 SNV: p.L288I .
Ishikawa (Heraklio) 02 ER SNV: p.P113T .
KPL1 SNV: p.P161T .
MCF7 SNV: p.P161T .
NCIH23 SNV: p.G158Ter .
SCC25 Deletion: p.K291SfsTer5 .
SHSY5Y SNV: p.Q141K .
SNU1 SNV: p.L406R .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 9 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
STPyne
 Probe Info  		K115(10.00); K126(5.88); K192(10.00); K30(5.68)  LDD0277  [1]
HHS-475
 Probe Info  		Y131(1.10); Y194(1.11)  LDD0264  [2]
DBIA
 Probe Info  		C391(0.75)  LDD0533  [3]
5E-2FA
 Probe Info  		H117(0.00); H132(0.00)  LDD2235  [4]
m-APA
 Probe Info  		N.A.  LDD2231  [4]
IA-alkyne
 Probe Info  		N.A.  LDD0162  [5]
Acrolein
 Probe Info  		N.A.  LDD0217  [6]
HHS-465
 Probe Info  		N.A.  LDD2240  [7]
HHS-482
 Probe Info  		Y131(1.50); Y194(0.98)  LDD2239  [8]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0216  AC100 HCT 116 C391(0.75)  LDD0533  [3]
 LDCM0217  AC101 HCT 116 C391(0.66)  LDD0534  [3]
 LDCM0218  AC102 HCT 116 C391(0.76)  LDD0535  [3]
 LDCM0219  AC103 HCT 116 C391(0.54)  LDD0536  [3]
 LDCM0220  AC104 HCT 116 C391(0.79)  LDD0537  [3]
 LDCM0221  AC105 HCT 116 C391(0.63)  LDD0538  [3]
 LDCM0222  AC106 HCT 116 C391(0.57)  LDD0539  [3]
 LDCM0223  AC107 HCT 116 C391(0.67)  LDD0540  [3]
 LDCM0224  AC108 HCT 116 C391(0.68)  LDD0541  [3]
 LDCM0225  AC109 HCT 116 C391(1.03)  LDD0542  [3]
 LDCM0227  AC110 HCT 116 C391(0.61)  LDD0544  [3]
 LDCM0228  AC111 HCT 116 C391(0.58)  LDD0545  [3]
 LDCM0229  AC112 HCT 116 C391(0.59)  LDD0546  [3]
 LDCM0230  AC113 HCT 116 C391(1.13)  LDD0547  [3]
 LDCM0231  AC114 HCT 116 C391(1.01)  LDD0548  [3]
 LDCM0232  AC115 HCT 116 C391(0.92)  LDD0549  [3]
 LDCM0233  AC116 HCT 116 C391(1.12)  LDD0550  [3]
 LDCM0234  AC117 HCT 116 C391(0.98)  LDD0551  [3]
 LDCM0235  AC118 HCT 116 C391(1.08)  LDD0552  [3]
 LDCM0236  AC119 HCT 116 C391(1.11)  LDD0553  [3]
 LDCM0238  AC120 HCT 116 C391(1.00)  LDD0555  [3]
 LDCM0239  AC121 HCT 116 C391(0.78)  LDD0556  [3]
 LDCM0240  AC122 HCT 116 C391(0.94)  LDD0557  [3]
 LDCM0241  AC123 HCT 116 C391(0.72)  LDD0558  [3]
 LDCM0242  AC124 HCT 116 C391(0.94)  LDD0559  [3]
 LDCM0243  AC125 HCT 116 C391(1.01)  LDD0560  [3]
 LDCM0244  AC126 HCT 116 C391(0.94)  LDD0561  [3]
 LDCM0245  AC127 HCT 116 C391(0.88)  LDD0562  [3]
 LDCM0263  AC143 HCT 116 C391(0.58)  LDD0580  [3]
 LDCM0264  AC144 HCT 116 C391(0.44)  LDD0581  [3]
 LDCM0265  AC145 HCT 116 C391(0.80)  LDD0582  [3]
 LDCM0266  AC146 HCT 116 C391(0.54)  LDD0583  [3]
 LDCM0267  AC147 HCT 116 C391(0.60)  LDD0584  [3]
 LDCM0268  AC148 HCT 116 C391(0.27)  LDD0585  [3]
 LDCM0269  AC149 HCT 116 C391(0.48)  LDD0586  [3]
 LDCM0271  AC150 HCT 116 C391(0.75)  LDD0588  [3]
 LDCM0272  AC151 HCT 116 C391(0.74)  LDD0589  [3]
 LDCM0273  AC152 HCT 116 C391(0.42)  LDD0590  [3]
 LDCM0274  AC153 HCT 116 C391(0.25)  LDD0591  [3]
 LDCM0621  AC154 HCT 116 C391(0.54)  LDD2158  [3]
 LDCM0622  AC155 HCT 116 C391(0.52)  LDD2159  [3]
 LDCM0623  AC156 HCT 116 C391(0.64)  LDD2160  [3]
 LDCM0624  AC157 HCT 116 C391(0.70)  LDD2161  [3]
 LDCM0296  AC35 HCT 116 C391(1.05)  LDD0613  [3]
 LDCM0297  AC36 HCT 116 C391(0.82)  LDD0614  [3]
 LDCM0298  AC37 HCT 116 C391(1.13)  LDD0615  [3]
 LDCM0299  AC38 HCT 116 C391(0.78)  LDD0616  [3]
 LDCM0300  AC39 HCT 116 C391(0.90)  LDD0617  [3]
 LDCM0302  AC40 HCT 116 C391(0.95)  LDD0619  [3]
 LDCM0303  AC41 HCT 116 C391(0.83)  LDD0620  [3]
 LDCM0304  AC42 HCT 116 C391(0.75)  LDD0621  [3]
 LDCM0305  AC43 HCT 116 C391(0.89)  LDD0622  [3]
 LDCM0306  AC44 HCT 116 C391(1.21)  LDD0623  [3]
 LDCM0307  AC45 HCT 116 C391(1.46)  LDD0624  [3]
 LDCM0320  AC57 HCT 116 C391(0.51)  LDD0637  [3]
 LDCM0321  AC58 HCT 116 C391(0.56)  LDD0638  [3]
 LDCM0322  AC59 HCT 116 C391(0.59)  LDD0639  [3]
 LDCM0324  AC60 HCT 116 C391(0.41)  LDD0641  [3]
 LDCM0325  AC61 HCT 116 C391(0.55)  LDD0642  [3]
 LDCM0326  AC62 HCT 116 C391(0.48)  LDD0643  [3]
 LDCM0327  AC63 HCT 116 C391(0.43)  LDD0644  [3]
 LDCM0328  AC64 HCT 116 C391(0.46)  LDD0645  [3]
 LDCM0329  AC65 HCT 116 C391(0.64)  LDD0646  [3]
 LDCM0330  AC66 HCT 116 C391(0.50)  LDD0647  [3]
 LDCM0331  AC67 HCT 116 C391(0.27)  LDD0648  [3]
 LDCM0332  AC68 HCT 116 C391(0.78)  LDD0649  [3]
 LDCM0333  AC69 HCT 116 C391(0.76)  LDD0650  [3]
 LDCM0335  AC70 HCT 116 C391(0.53)  LDD0652  [3]
 LDCM0336  AC71 HCT 116 C391(0.90)  LDD0653  [3]
 LDCM0337  AC72 HCT 116 C391(0.83)  LDD0654  [3]
 LDCM0338  AC73 HCT 116 C391(0.44)  LDD0655  [3]
 LDCM0339  AC74 HCT 116 C391(0.46)  LDD0656  [3]
 LDCM0340  AC75 HCT 116 C391(0.41)  LDD0657  [3]
 LDCM0341  AC76 HCT 116 C391(0.70)  LDD0658  [3]
 LDCM0342  AC77 HCT 116 C391(0.62)  LDD0659  [3]
 LDCM0343  AC78 HCT 116 C391(0.79)  LDD0660  [3]
 LDCM0344  AC79 HCT 116 C391(1.04)  LDD0661  [3]
 LDCM0346  AC80 HCT 116 C391(0.76)  LDD0663  [3]
 LDCM0347  AC81 HCT 116 C391(0.83)  LDD0664  [3]
 LDCM0348  AC82 HCT 116 C391(0.54)  LDD0665  [3]
 LDCM0349  AC83 HCT 116 C391(4.12)  LDD0666  [3]
 LDCM0350  AC84 HCT 116 C391(3.66)  LDD0667  [3]
 LDCM0351  AC85 HCT 116 C391(3.90)  LDD0668  [3]
 LDCM0352  AC86 HCT 116 C391(4.51)  LDD0669  [3]
 LDCM0353  AC87 HCT 116 C391(3.91)  LDD0670  [3]
 LDCM0354  AC88 HCT 116 C391(3.24)  LDD0671  [3]
 LDCM0355  AC89 HCT 116 C391(3.91)  LDD0672  [3]
 LDCM0357  AC90 HCT 116 C391(4.09)  LDD0674  [3]
 LDCM0358  AC91 HCT 116 C391(4.56)  LDD0675  [3]
 LDCM0359  AC92 HCT 116 C391(5.65)  LDD0676  [3]
 LDCM0360  AC93 HCT 116 C391(2.75)  LDD0677  [3]
 LDCM0361  AC94 HCT 116 C391(3.20)  LDD0678  [3]
 LDCM0362  AC95 HCT 116 C391(3.29)  LDD0679  [3]
 LDCM0363  AC96 HCT 116 C391(3.68)  LDD0680  [3]
 LDCM0364  AC97 HCT 116 C391(4.09)  LDD0681  [3]
 LDCM0365  AC98 HCT 116 C391(0.62)  LDD0682  [3]
 LDCM0366  AC99 HCT 116 C391(0.62)  LDD0683  [3]
 LDCM0108  Chloroacetamide HeLa N.A.  LDD0222  [6]
 LDCM0387  CL117 HCT 116 C391(0.71)  LDD0704  [3]
 LDCM0388  CL118 HCT 116 C391(0.94)  LDD0705  [3]
 LDCM0389  CL119 HCT 116 C391(0.86)  LDD0706  [3]
 LDCM0391  CL120 HCT 116 C391(0.78)  LDD0708  [3]
 LDCM0396  CL125 HCT 116 C391(0.89)  LDD0713  [3]
 LDCM0397  CL126 HCT 116 C391(1.06)  LDD0714  [3]
 LDCM0398  CL127 HCT 116 C391(0.93)  LDD0715  [3]
 LDCM0399  CL128 HCT 116 C391(0.59)  LDD0716  [3]
 LDCM0403  CL16 HCT 116 C391(0.90)  LDD0720  [3]
 LDCM0404  CL17 HCT 116 C391(0.97)  LDD0721  [3]
 LDCM0405  CL18 HCT 116 C391(0.66)  LDD0722  [3]
 LDCM0406  CL19 HCT 116 C391(0.68)  LDD0723  [3]
 LDCM0408  CL20 HCT 116 C391(0.71)  LDD0725  [3]
 LDCM0409  CL21 HCT 116 C391(0.56)  LDD0726  [3]
 LDCM0410  CL22 HCT 116 C391(0.40)  LDD0727  [3]
 LDCM0411  CL23 HCT 116 C391(0.86)  LDD0728  [3]
 LDCM0412  CL24 HCT 116 C391(0.56)  LDD0729  [3]
 LDCM0413  CL25 HCT 116 C391(0.61)  LDD0730  [3]
 LDCM0414  CL26 HCT 116 C391(0.85)  LDD0731  [3]
 LDCM0415  CL27 HCT 116 C391(0.85)  LDD0732  [3]
 LDCM0416  CL28 HCT 116 C391(0.68)  LDD0733  [3]
 LDCM0417  CL29 HCT 116 C391(0.71)  LDD0734  [3]
 LDCM0419  CL30 HCT 116 C391(0.95)  LDD0736  [3]
 LDCM0116  HHS-0101 DM93 Y131(1.10); Y194(1.11)  LDD0264  [2]
 LDCM0117  HHS-0201 DM93 Y194(0.79); Y131(1.12)  LDD0265  [2]
 LDCM0118  HHS-0301 DM93 Y131(1.27)  LDD0266  [2]
 LDCM0119  HHS-0401 DM93 Y194(1.03); Y131(1.12)  LDD0267  [2]
 LDCM0120  HHS-0701 DM93 Y131(0.80); Y194(5.27)  LDD0268  [2]
 LDCM0107  IAA HeLa N.A.  LDD0221  [6]
 LDCM0022  KB02 BRX211 C391(1.64)  LDD2268  [9]
 LDCM0023  KB03 BRX211 C391(1.61)  LDD2685  [9]
 LDCM0024  KB05 SNU-245 C391(1.50)  LDD3440  [9]
 LDCM0109  NEM HeLa N.A.  LDD0223  [6]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 10 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
5-aminolevulinate synthase, non-specific, mitochondrial (ALAS1) Class-II pyridoxal-phosphate-dependent aminotransferase family P13196
Serine/threonine-protein phosphatase PP1-alpha catalytic subunit (PPP1CA) PPP phosphatase family P62136
Serine/threonine-protein phosphatase PP1-beta catalytic subunit (PPP1CB) PPP phosphatase family P62140
Peripheral plasma membrane protein CASK (CASK) CAMK Ser/Thr protein kinase family O14936
E3 ubiquitin-protein ligase TRIM23 (TRIM23) Arf family P36406
TNF receptor-associated factor 2 (TRAF2) TNF receptor-associated factor family Q12933
Zinc finger protein RFP (TRIM27) TRIM/RBCC family P14373
Protein yippee-like 2 (YPEL2) Yippee family Q96QA6
Fibronectin type III and SPRY domain-containing protein 2 (FSD2) . A1L4K1
Probable E3 ubiquitin-protein ligase TRIML2 (TRIML2) . Q8N7C3
Transcription factor
Click To Hide/Show 6 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
G-protein coupled receptor-associated sorting protein 2 (GPRASP2) GPRASP family Q96D09
DNA-binding protein Ikaros (IKZF1) Ikaros C2H2-type zinc-finger protein family Q13422
Zinc finger and BTB domain-containing protein 16 (ZBTB16) Krueppel C2H2-type zinc-finger protein family Q05516
Zinc finger protein 655 (ZNF655) Krueppel C2H2-type zinc-finger protein family Q8N720
Zinc finger protein 774 (ZNF774) Krueppel C2H2-type zinc-finger protein family Q6NX45
Homeobox-containing protein 1 (HMBOX1) . Q6NT76
Other
Click To Hide/Show 49 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Drebrin-like protein (DBNL) ABP1 family Q9UJU6
Activity-regulated cytoskeleton-associated protein (ARC) ARC/ARG3.1 family Q7LC44
Biogenesis of lysosome-related organelles complex 1 subunit 2 (BLOC1S2) BLOC1S2 family Q6QNY1
cAMP-dependent protein kinase type I-beta regulatory subunit (PRKAR1B) CAMP-dependent kinase regulatory chain family P31321
Coiled-coil domain-containing protein 172 (CCDC172) CCDC172 family P0C7W6
Coiled-coil domain-containing protein 88B (CCDC88B) CCDC88 family A6NC98
Cerebellar degeneration-related protein 2 (CDR2) CDR2 family Q01850
Cerebellar degeneration-related protein 2-like (CDR2L) CDR2 family Q86X02
Centrosomal protein of 76 kDa (CEP76) CEP76 family Q8TAP6
Cilia- and flagella-associated protein 53 (CFAP53) CFAP53 family Q96M91
Endophilin-A2 (SH3GL1) Endophilin family Q99961
Golgin subfamily A member 2 (GOLGA2) GOLGA2 family Q08379
Golgin subfamily A member 6-like protein 9 (GOLGA6L9) GOLGA6 family A6NEM1
GRB2-related adapter protein 2 (GRAP2) GRB2/sem-5/DRK family O75791
Growth factor receptor-bound protein 2 (GRB2) GRB2/sem-5/DRK family P62993
Keratin, type I cytoskeletal 40 (KRT40) Intermediate filament family Q6A162
Protein LDOC1 (LDOC1) LDOC1 family O95751
Leucine zipper putative tumor suppressor 2 (LZTS2) LZTS2 family Q9BRK4
Methyl-CpG-binding domain protein 3-like 1 (MBD3L1) MBD3L family Q8WWY6
Harmonin-binding protein USHBP1 (USHBP1) MCC family Q8N6Y0
MORF4 family-associated protein 1 (MRFAP1) MORF4 family-associated protein family Q9Y605
Microtubule-associated tumor suppressor candidate 2 (MTUS2) MTUS1 family Q5JR59
NUT family member 1 (NUTM1) NUT family Q86Y26
Paraneoplastic antigen Ma1 (PNMA1) PNMA family Q8ND90
RAD50-interacting protein 1 (RINT1) RINT1 family Q6NUQ1
Src kinase-associated phosphoprotein 1 (SKAP1) SKAP family Q86WV1
Signal transducing adapter molecule 2 (STAM2) STAM family O75886
Syntaxin-11 (STX11) Syntaxin family O75558
Transforming acidic coiled-coil-containing protein 1 (TACC1) TACC family O75410
Tuftelin-interacting protein 11 (TFIP11) TFP11/STIP family Q9UBB9
Tetratricopeptide repeat protein 19, mitochondrial (TTC19) TTC19 family Q6DKK2
Vacuolar protein sorting-associated protein 52 homolog (VPS52) VPS52 family Q8N1B4
Centrosomal protein of 70 kDa (CEP70) . Q8NHQ1
GRAM domain-containing protein 4 (GRAMD4) . Q6IC98
Heat shock factor 2-binding protein (HSF2BP) . O75031
Hematopoietic lineage cell-specific protein (HCLS1) . P14317
Melanoma-associated antigen 11 (MAGEA11) . P43364
Melanoma-associated antigen 6 (MAGEA6) . P43360
MORN repeat-containing protein 3 (MORN3) . Q6PF18
Myomegalin (PDE4DIP) . Q5VU43
Ninein-like protein (NINL) . Q9Y2I6
PRKCA-binding protein (PICK1) . Q9NRD5
Proline-serine-threonine phosphatase-interacting protein 1 (PSTPIP1) . O43586
Puratrophin-1 (PLEKHG4) . Q58EX7
Sorbin and SH3 domain-containing protein 2 (SORBS2) . O94875
Sperm-associated antigen 5 (SPAG5) . Q96R06
UBX domain-containing protein 11 (UBXN11) . Q5T124
Vinexin (SORBS3) . O60504
Zinc finger MYND domain-containing protein 12 (ZMYND12) . Q9H0C1

References

1 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
2 Discovery of a Cell-Active SuTEx Ligand of Prostaglandin Reductase 2. Chembiochem. 2021 Jun 15;22(12):2134-2139. doi: 10.1002/cbic.202000879. Epub 2021 Apr 29.
3 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
4 Global profiling of functional histidines in live cells using small-molecule photosensitizer and chemical probe relay labelling. Nat Chem. 2024 Jun 4. doi: 10.1038/s41557-024-01545-6. Online ahead of print.
Mass spectrometry data entry: PXD042377
5 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
6 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
7 Global profiling identifies a stress-responsive tyrosine site on EDC3 regulating biomolecular condensate formation. Cell Chem Biol. 2022 Dec 15;29(12):1709-1720.e7. doi: 10.1016/j.chembiol.2022.11.008. Epub 2022 Dec 6.
Mass spectrometry data entry: PXD038010
8 Global targeting of functional tyrosines using sulfur-triazole exchange chemistry. Nat Chem Biol. 2020 Feb;16(2):150-159. doi: 10.1038/s41589-019-0404-5. Epub 2019 Nov 25.
9 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840