Details of the Target

General Information of Target

Target ID LDTP11344
Target Name Single-stranded DNA-binding protein 3 (SSBP3)
Gene Name SSBP3
Gene ID 23648
Synonyms
SSDP; SSDP1; Single-stranded DNA-binding protein 3; Sequence-specific single-stranded-DNA-binding protein
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MRGRDLCSSTQSQALGSLRTTTPAFTLNIPSEANHTEQPPAGLGARLQEAGVSIPPRRGR
PTPTLEKKKKPHLMAEDEPSGALLKPLVFRVDETTPAVVQSVLLERGWNKFDKQEQNAED
WNLYWRTSSFRMTEHNSVKPWQQLNHHPGTTKLTRKDCLAKHLKHMRRMYGTSLYQFIPL
TFVMPNDYTKFVAEYFQERQMLGTKHSYWICKPAELSRGRGILIFSDFKDFIFDDMYIVQ
KYISNPLLIGRYKCDLRIYVCVTGFKPLTIYVYQEGLVRFATEKFDLSNLQNNYAHLTNS
SINKSGASYEKIKEVIGHGCKWTLSRFFSYLRSWDVDDLLLWKKIHRMVILTILAIAPSV
PFAANCFELFGFDILIDDNLKPWLLEVNYSPALTLDCSTDVLVKRKLVHDIIDLIYLNGL
RNEGREASNATHGNSNIDAAKSDRGGLDAPDCLPYDSLSFTSRMYNEDDSVVEKAVSVRP
EAAPASQLEGEMSGQDFHLSTREMPQSKPKLRSRHTPHKTLMPYASLFQSHSCKTKTSPC
VLSDRGKAPDPQAGNFVLVFPFNEATLGASRNGLNVKRIIQELQKLMNKQHS
Target Bioclass
Other
Subcellular location
Nucleus
Function May be involved in transcription regulation of the alpha 2(I) collagen gene where it binds to the single-stranded polypyrimidine sequences in the promoter region.
Uniprot ID
Q9BWW4
Ensemble ID
ENST00000357475.9
HGNC ID
HGNC:15674

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
COLO792 SNV: p.G305S .
IM95 SNV: p.A285T .
MCC13 SNV: p.P319L DBIA    Probe Info 
MOLT4 SNV: p.G53R IA-alkyne    Probe Info 
NCIH146 SNV: p.P175L .
NCIH358 Substitution: p.G322C .
RKO SNV: p.I50T .
SH4 SNV: p.G324C .
SUPT1 Deletion: p.N49TfsTer95 DBIA    Probe Info 
TOV21G SNV: p.P171T .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 13 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C80(1.72)  LDD3314  [1]
AHL-Pu-1
 Probe Info  		C80(2.06)  LDD0168  [2]
m-APA
 Probe Info  		N.A.  LDD2231  [3]
4-Iodoacetamidophenylacetylene
 Probe Info  		N.A.  LDD0038  [4]
IA-alkyne
 Probe Info  		N.A.  LDD0036  [4]
Lodoacetamide azide
 Probe Info  		N.A.  LDD0037  [4]
Compound 10
 Probe Info  		N.A.  LDD2216  [5]
IPM
 Probe Info  		N.A.  LDD0147  [6]
TFBX
 Probe Info  		N.A.  LDD0148  [6]
Phosphinate-6
 Probe Info  		N.A.  LDD0018  [7]
Methacrolein
 Probe Info  		N.A.  LDD0218  [8]
AOyne
 Probe Info  		12.30  LDD0443  [9]
NAIA_5
 Probe Info  		N.A.  LDD2223  [10]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0025  4SU-RNA HEK-293T C80(2.06)  LDD0168  [2]
 LDCM0026  4SU-RNA+native RNA HEK-293T C80(2.42)  LDD0169  [2]
 LDCM0214  AC1 HEK-293T C80(1.11)  LDD1507  [11]
 LDCM0226  AC11 HEK-293T C80(1.15)  LDD1509  [11]
 LDCM0237  AC12 HEK-293T C80(1.26)  LDD1510  [11]
 LDCM0270  AC15 HEK-293T C80(1.29)  LDD1513  [11]
 LDCM0276  AC17 HEK-293T C80(1.22)  LDD1515  [11]
 LDCM0278  AC19 HEK-293T C80(1.24)  LDD1517  [11]
 LDCM0280  AC20 HEK-293T C80(1.37)  LDD1519  [11]
 LDCM0281  AC21 HEK-293T C80(1.08)  LDD1520  [11]
 LDCM0283  AC23 HEK-293T C80(1.54)  LDD1522  [11]
 LDCM0284  AC24 HEK-293T C80(1.27)  LDD1523  [11]
 LDCM0285  AC25 HEK-293T C80(1.21)  LDD1524  [11]
 LDCM0287  AC27 HEK-293T C80(1.27)  LDD1526  [11]
 LDCM0288  AC28 HEK-293T C80(1.24)  LDD1527  [11]
 LDCM0289  AC29 HEK-293T C80(1.06)  LDD1528  [11]
 LDCM0290  AC3 HEK-293T C80(1.01)  LDD1529  [11]
 LDCM0292  AC31 HEK-293T C80(1.19)  LDD1531  [11]
 LDCM0293  AC32 HEK-293T C80(1.19)  LDD1532  [11]
 LDCM0294  AC33 HEK-293T C80(1.95)  LDD1533  [11]
 LDCM0296  AC35 HEK-293T C80(1.39)  LDD1535  [11]
 LDCM0297  AC36 HEK-293T C80(1.42)  LDD1536  [11]
 LDCM0298  AC37 HEK-293T C80(1.12)  LDD1537  [11]
 LDCM0300  AC39 HEK-293T C80(1.46)  LDD1539  [11]
 LDCM0301  AC4 HEK-293T C80(1.03)  LDD1540  [11]
 LDCM0302  AC40 HEK-293T C80(1.24)  LDD1541  [11]
 LDCM0303  AC41 HEK-293T C80(1.46)  LDD1542  [11]
 LDCM0305  AC43 HEK-293T C80(1.30)  LDD1544  [11]
 LDCM0306  AC44 HEK-293T C80(1.27)  LDD1545  [11]
 LDCM0307  AC45 HEK-293T C80(1.08)  LDD1546  [11]
 LDCM0309  AC47 HEK-293T C80(1.43)  LDD1548  [11]
 LDCM0310  AC48 HEK-293T C80(1.32)  LDD1549  [11]
 LDCM0311  AC49 HEK-293T C80(1.33)  LDD1550  [11]
 LDCM0312  AC5 HEK-293T C80(0.97)  LDD1551  [11]
 LDCM0314  AC51 HEK-293T C80(1.27)  LDD1553  [11]
 LDCM0315  AC52 HEK-293T C80(1.24)  LDD1554  [11]
 LDCM0316  AC53 HEK-293T C80(1.16)  LDD1555  [11]
 LDCM0318  AC55 HEK-293T C80(1.35)  LDD1557  [11]
 LDCM0319  AC56 HEK-293T C80(1.23)  LDD1558  [11]
 LDCM0320  AC57 HEK-293T C80(1.18)  LDD1559  [11]
 LDCM0322  AC59 HEK-293T C80(1.36)  LDD1561  [11]
 LDCM0324  AC60 HEK-293T C80(1.26)  LDD1563  [11]
 LDCM0325  AC61 HEK-293T C80(1.01)  LDD1564  [11]
 LDCM0327  AC63 HEK-293T C80(1.35)  LDD1566  [11]
 LDCM0328  AC64 HEK-293T C80(1.23)  LDD1567  [11]
 LDCM0334  AC7 HEK-293T C80(1.28)  LDD1568  [11]
 LDCM0345  AC8 HEK-293T C80(0.92)  LDD1569  [11]
 LDCM0248  AKOS034007472 HEK-293T C80(1.06)  LDD1511  [11]
 LDCM0356  AKOS034007680 HEK-293T C80(1.44)  LDD1570  [11]
 LDCM0275  AKOS034007705 HEK-293T C80(1.12)  LDD1514  [11]
 LDCM0632  CL-Sc Hep-G2 C80(1.00)  LDD2227  [10]
 LDCM0367  CL1 HEK-293T C80(0.96)  LDD1571  [11]
 LDCM0370  CL101 HEK-293T C80(1.15)  LDD1574  [11]
 LDCM0371  CL102 HEK-293T C80(1.17)  LDD1575  [11]
 LDCM0372  CL103 HEK-293T C80(1.32)  LDD1576  [11]
 LDCM0374  CL105 HEK-293T C80(1.20)  LDD1578  [11]
 LDCM0375  CL106 HEK-293T C80(1.15)  LDD1579  [11]
 LDCM0376  CL107 HEK-293T C80(1.14)  LDD1580  [11]
 LDCM0378  CL109 HEK-293T C80(1.16)  LDD1582  [11]
 LDCM0379  CL11 HEK-293T C80(1.18)  LDD1583  [11]
 LDCM0380  CL110 HEK-293T C80(1.09)  LDD1584  [11]
 LDCM0381  CL111 HEK-293T C80(1.13)  LDD1585  [11]
 LDCM0383  CL113 HEK-293T C80(1.21)  LDD1587  [11]
 LDCM0384  CL114 HEK-293T C80(1.32)  LDD1588  [11]
 LDCM0385  CL115 HEK-293T C80(1.32)  LDD1589  [11]
 LDCM0387  CL117 HEK-293T C80(1.23)  LDD1591  [11]
 LDCM0388  CL118 HEK-293T C80(1.29)  LDD1592  [11]
 LDCM0389  CL119 HEK-293T C80(1.20)  LDD1593  [11]
 LDCM0390  CL12 HEK-293T C80(1.18)  LDD1594  [11]
 LDCM0392  CL121 HEK-293T C80(1.19)  LDD1596  [11]
 LDCM0393  CL122 HEK-293T C80(1.16)  LDD1597  [11]
 LDCM0394  CL123 HEK-293T C80(1.12)  LDD1598  [11]
 LDCM0396  CL125 HEK-293T C80(0.98)  LDD1600  [11]
 LDCM0397  CL126 HEK-293T C80(1.22)  LDD1601  [11]
 LDCM0398  CL127 HEK-293T C80(1.13)  LDD1602  [11]
 LDCM0400  CL13 HEK-293T C80(0.96)  LDD1604  [11]
 LDCM0401  CL14 HEK-293T C80(1.25)  LDD1605  [11]
 LDCM0402  CL15 HEK-293T C80(1.19)  LDD1606  [11]
 LDCM0404  CL17 HEK-293T C80(1.28)  LDD1608  [11]
 LDCM0406  CL19 HEK-293T C80(1.20)  LDD1610  [11]
 LDCM0407  CL2 HEK-293T C80(1.07)  LDD1611  [11]
 LDCM0408  CL20 HEK-293T C80(1.22)  LDD1612  [11]
 LDCM0409  CL21 HEK-293T C80(1.23)  LDD1613  [11]
 LDCM0411  CL23 HEK-293T C80(1.50)  LDD1615  [11]
 LDCM0412  CL24 HEK-293T C80(1.19)  LDD1616  [11]
 LDCM0413  CL25 HEK-293T C80(0.98)  LDD1617  [11]
 LDCM0414  CL26 HEK-293T C80(1.27)  LDD1618  [11]
 LDCM0415  CL27 HEK-293T C80(1.19)  LDD1619  [11]
 LDCM0417  CL29 HEK-293T C80(1.22)  LDD1621  [11]
 LDCM0418  CL3 HEK-293T C80(1.23)  LDD1622  [11]
 LDCM0420  CL31 HEK-293T C80(1.26)  LDD1624  [11]
 LDCM0421  CL32 HEK-293T C80(1.14)  LDD1625  [11]
 LDCM0422  CL33 HEK-293T C80(0.99)  LDD1626  [11]
 LDCM0424  CL35 HEK-293T C80(1.52)  LDD1628  [11]
 LDCM0425  CL36 HEK-293T C80(1.19)  LDD1629  [11]
 LDCM0426  CL37 HEK-293T C80(1.05)  LDD1630  [11]
 LDCM0428  CL39 HEK-293T C80(1.25)  LDD1632  [11]
 LDCM0431  CL41 HEK-293T C80(1.94)  LDD1635  [11]
 LDCM0433  CL43 HEK-293T C80(1.26)  LDD1637  [11]
 LDCM0434  CL44 HEK-293T C80(1.25)  LDD1638  [11]
 LDCM0435  CL45 HEK-293T C80(1.13)  LDD1639  [11]
 LDCM0437  CL47 HEK-293T C80(1.42)  LDD1641  [11]
 LDCM0438  CL48 HEK-293T C80(1.18)  LDD1642  [11]
 LDCM0439  CL49 HEK-293T C80(1.00)  LDD1643  [11]
 LDCM0440  CL5 HEK-293T C80(1.27)  LDD1644  [11]
 LDCM0441  CL50 HEK-293T C80(1.32)  LDD1645  [11]
 LDCM0444  CL53 HEK-293T C80(1.39)  LDD1647  [11]
 LDCM0446  CL55 HEK-293T C80(1.31)  LDD1649  [11]
 LDCM0447  CL56 HEK-293T C80(1.35)  LDD1650  [11]
 LDCM0448  CL57 HEK-293T C80(1.15)  LDD1651  [11]
 LDCM0450  CL59 HEK-293T C80(1.48)  LDD1653  [11]
 LDCM0452  CL60 HEK-293T C80(1.33)  LDD1655  [11]
 LDCM0453  CL61 HEK-293T C80(1.12)  LDD1656  [11]
 LDCM0454  CL62 HEK-293T C80(1.32)  LDD1657  [11]
 LDCM0455  CL63 HEK-293T C80(1.31)  LDD1658  [11]
 LDCM0457  CL65 HEK-293T C80(1.78)  LDD1660  [11]
 LDCM0459  CL67 HEK-293T C80(1.37)  LDD1662  [11]
 LDCM0460  CL68 HEK-293T C80(1.29)  LDD1663  [11]
 LDCM0461  CL69 HEK-293T C80(1.11)  LDD1664  [11]
 LDCM0462  CL7 HEK-293T C80(1.13)  LDD1665  [11]
 LDCM0464  CL71 HEK-293T C80(1.35)  LDD1667  [11]
 LDCM0465  CL72 HEK-293T C80(1.28)  LDD1668  [11]
 LDCM0466  CL73 HEK-293T C80(0.95)  LDD1669  [11]
 LDCM0467  CL74 HEK-293T C80(1.17)  LDD1670  [11]
 LDCM0470  CL77 HEK-293T C80(1.50)  LDD1673  [11]
 LDCM0472  CL79 HEK-293T C80(1.13)  LDD1675  [11]
 LDCM0473  CL8 HEK-293T C80(0.98)  LDD1676  [11]
 LDCM0474  CL80 HEK-293T C80(1.06)  LDD1677  [11]
 LDCM0475  CL81 HEK-293T C80(1.02)  LDD1678  [11]
 LDCM0477  CL83 HEK-293T C80(1.10)  LDD1680  [11]
 LDCM0478  CL84 HEK-293T C80(1.00)  LDD1681  [11]
 LDCM0479  CL85 HEK-293T C80(0.84)  LDD1682  [11]
 LDCM0480  CL86 HEK-293T C80(0.91)  LDD1683  [11]
 LDCM0481  CL87 HEK-293T C80(0.92)  LDD1684  [11]
 LDCM0483  CL89 HEK-293T C80(0.94)  LDD1686  [11]
 LDCM0484  CL9 HEK-293T C80(0.78)  LDD1687  [11]
 LDCM0486  CL91 HEK-293T C80(1.18)  LDD1689  [11]
 LDCM0487  CL92 HEK-293T C80(1.19)  LDD1690  [11]
 LDCM0488  CL93 HEK-293T C80(0.98)  LDD1691  [11]
 LDCM0490  CL95 HEK-293T C80(1.25)  LDD1693  [11]
 LDCM0491  CL96 HEK-293T C80(0.83)  LDD1694  [11]
 LDCM0492  CL97 HEK-293T C80(1.08)  LDD1695  [11]
 LDCM0493  CL98 HEK-293T C80(1.22)  LDD1696  [11]
 LDCM0494  CL99 HEK-293T C80(1.14)  LDD1697  [11]
 LDCM0495  E2913 HEK-293T C80(1.32)  LDD1698  [11]
 LDCM0573  Fragment11 Ramos C80(20.00)  LDD2190  [12]
 LDCM0576  Fragment14 Ramos C80(0.58)  LDD2193  [12]
 LDCM0586  Fragment28 Ramos C80(0.40)  LDD2198  [12]
 LDCM0468  Fragment33 HEK-293T C80(1.04)  LDD1671  [11]
 LDCM0427  Fragment51 HEK-293T C80(1.23)  LDD1631  [11]
 LDCM0022  KB02 697 C80(1.93)  LDD2245  [1]
 LDCM0023  KB03 Ramos C80(0.43)  LDD2183  [12]
 LDCM0024  KB05 IGR37 C80(1.72)  LDD3314  [1]
 LDCM0627  NUDT7-COV-1 HEK-293T C80(0.64)  LDD2206  [13]
 LDCM0628  OTUB2-COV-1 HEK-293T C80(1.18)  LDD2207  [13]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 3 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Tyrosine-protein kinase Blk (BLK) Tyr protein kinase family P51451
Tyrosine-protein kinase Yes (YES1) Tyr protein kinase family P07947
Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1 (PIN1) . Q13526
Cytokine and receptor
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Interleukin-36 receptor antagonist protein (IL36RN) IL-1 family Q9UBH0
Other
Click To Hide/Show 5 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
LIM domain-binding protein 1 (LDB1) LDB family Q86U70
LIM domain-binding protein 2 (LDB2) LDB family O43679
RNA-binding protein EWS (EWSR1) RRM TET family Q01844
NTF2-related export protein 2 (NXT2) . Q9NPJ8
TBC1 domain family member 23 (TBC1D23) . Q9NUY8

References

1 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
2 Chemoproteomic capture of RNA binding activity in living cells. Nat Commun. 2023 Oct 7;14(1):6282. doi: 10.1038/s41467-023-41844-z.
Mass spectrometry data entry: PXD044625
3 Global profiling of functional histidines in live cells using small-molecule photosensitizer and chemical probe relay labelling. Nat Chem. 2024 Jun 4. doi: 10.1038/s41557-024-01545-6. Online ahead of print.
Mass spectrometry data entry: PXD042377
4 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
5 Multiplexed CuAAC Suzuki-Miyaura Labeling for Tandem Activity-Based Chemoproteomic Profiling. Anal Chem. 2021 Feb 2;93(4):2610-2618. doi: 10.1021/acs.analchem.0c04726. Epub 2021 Jan 20.
Mass spectrometry data entry: PXD022279
6 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
7 DFT-Guided Discovery of Ethynyl-Triazolyl-Phosphinates as Modular Electrophiles for Chemoselective Cysteine Bioconjugation and Profiling. Angew Chem Int Ed Engl. 2022 Oct 10;61(41):e202205348. doi: 10.1002/anie.202205348. Epub 2022 Aug 22.
Mass spectrometry data entry: PXD033004
8 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
9 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.
10 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
11 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
12 Site-specific quantitative cysteine profiling with data-independent acquisition-based mass spectrometry. Methods Enzymol. 2023;679:295-322. doi: 10.1016/bs.mie.2022.07.037. Epub 2022 Sep 7.
Mass spectrometry data entry: PXD027578
13 Rapid Covalent-Probe Discovery by Electrophile-Fragment Screening. J Am Chem Soc. 2019 Jun 5;141(22):8951-8968. doi: 10.1021/jacs.9b02822. Epub 2019 May 22.